Salinity severely affects plant growth and development.Thus,it is crucial to identify the genes functioning in salt stress response and unravel the mechanism by which plants against salt stress.This study used the pho...Salinity severely affects plant growth and development.Thus,it is crucial to identify the genes functioning in salt stress response and unravel the mechanism by which plants against salt stress.This study used the phosphoproteomic assay and found that 123 of the 4000 quantitative analyzed phosphopeptides were induced by salt stress.The functional annotation of the non-redundant protein database(NR)showed 23 differentially expressed transcription factors,including a phosphopeptide covering the Serine 31 in the RAV(related to ABI3/VP1)transcription factor(named SiRAV1).SiRAV1 was located in the nucleus.Phenotypic and physiological analysis showed that overexpressing SiRAV1 in foxtail millet enhanced salt tolerance and alleviated the salt-induced increases of H_(2)O_(2) accumulation,malondialdehyde(MDA)content,and percent of electrolyte leakage.Further analysis showed that SiRAV1 positively regulated SiCAT expression to modulate the catalase(CAT)activity by directly binding to the SiCAT promoter in vivo and in vitro.Moreover,we found that phosphorylation of SiRAV1 at the Ser31 site positively regulated salt tolerance in foxtail millet via enhancing its binding ability to SiCAT promoter but did not affect its subcellular localization.Overall,our results define a mechanism for SiRAV1 function in salt response where salt-triggered phosphorylation of SiRAV1 at Ser31 enhances its binding ability to SiCAT promoter,and the increased SiCAT expression contributes to salt tolerance in foxtail millet.展开更多
A diode pumped alkali vapor laser has advantages in high quantum efficiency,excellent beam quality,and low thermal effect.We obtain a laser diode array(LDA)with linewidth of 0.20 nm and an external cavity of volume Br...A diode pumped alkali vapor laser has advantages in high quantum efficiency,excellent beam quality,and low thermal effect.We obtain a laser diode array(LDA)with linewidth of 0.20 nm and an external cavity of volume Bragg grating(VBG).Its central wavelength can be tuned from 779.30 nm to 780.10 nm by changing the VBG's temperature.Pumped by this LDA,a 2.1 W rubidium vapor laser is achieved with optical efficiency of 10.5%and slope efficiency of 22.7%.展开更多
We report a cesium vapor laser with fundamental mode output and a wavelength of 894 nm. The laser is pumped by a laser diode array with an external cavity of a holographic grating by using Littrow＇s structure. A slop...We report a cesium vapor laser with fundamental mode output and a wavelength of 894 nm. The laser is pumped by a laser diode array with an external cavity of a holographic grating by using Littrow＇s structure. A slope efbciency of 22.4% is obtained by using a pumping source with a linewidth of 0.26 nm and 80 kPa methane as the buffer gas. The threshold pumping power is 1.56 W.展开更多
基金This work was funded by the National Natural Science Foundation of China(31902062)the South China Botanical Garden,Chinese Academy of Sciences(QNXM-02).
文摘Salinity severely affects plant growth and development.Thus,it is crucial to identify the genes functioning in salt stress response and unravel the mechanism by which plants against salt stress.This study used the phosphoproteomic assay and found that 123 of the 4000 quantitative analyzed phosphopeptides were induced by salt stress.The functional annotation of the non-redundant protein database(NR)showed 23 differentially expressed transcription factors,including a phosphopeptide covering the Serine 31 in the RAV(related to ABI3/VP1)transcription factor(named SiRAV1).SiRAV1 was located in the nucleus.Phenotypic and physiological analysis showed that overexpressing SiRAV1 in foxtail millet enhanced salt tolerance and alleviated the salt-induced increases of H_(2)O_(2) accumulation,malondialdehyde(MDA)content,and percent of electrolyte leakage.Further analysis showed that SiRAV1 positively regulated SiCAT expression to modulate the catalase(CAT)activity by directly binding to the SiCAT promoter in vivo and in vitro.Moreover,we found that phosphorylation of SiRAV1 at the Ser31 site positively regulated salt tolerance in foxtail millet via enhancing its binding ability to SiCAT promoter but did not affect its subcellular localization.Overall,our results define a mechanism for SiRAV1 function in salt response where salt-triggered phosphorylation of SiRAV1 at Ser31 enhances its binding ability to SiCAT promoter,and the increased SiCAT expression contributes to salt tolerance in foxtail millet.
文摘A diode pumped alkali vapor laser has advantages in high quantum efficiency,excellent beam quality,and low thermal effect.We obtain a laser diode array(LDA)with linewidth of 0.20 nm and an external cavity of volume Bragg grating(VBG).Its central wavelength can be tuned from 779.30 nm to 780.10 nm by changing the VBG's temperature.Pumped by this LDA,a 2.1 W rubidium vapor laser is achieved with optical efficiency of 10.5%and slope efficiency of 22.7%.
文摘We report a cesium vapor laser with fundamental mode output and a wavelength of 894 nm. The laser is pumped by a laser diode array with an external cavity of a holographic grating by using Littrow＇s structure. A slope efbciency of 22.4% is obtained by using a pumping source with a linewidth of 0.26 nm and 80 kPa methane as the buffer gas. The threshold pumping power is 1.56 W.
