为了研究注射外源性神经介素B(NMB)对小鼠睾丸中NMB及其受体和睾酮合成关键酶(St AR、CYP11A1和3β-HSD)表达及血清中睾酮浓度的影响,试验将40只雄性ICR小鼠随机分成12 h对照组(12 h Control组)、12 h试验组(12 h NMB组)、24 h对照组(24...为了研究注射外源性神经介素B(NMB)对小鼠睾丸中NMB及其受体和睾酮合成关键酶(St AR、CYP11A1和3β-HSD)表达及血清中睾酮浓度的影响,试验将40只雄性ICR小鼠随机分成12 h对照组(12 h Control组)、12 h试验组(12 h NMB组)、24 h对照组(24 h Control组)和24 h试验组(24 h NMB组),每组10只,2个对照组和2个试验组分别按体重10μL/g腹腔注射生理盐水和10 nmol/L NMB溶液;称量注射前后小鼠体重,计算饲料用量,并观察小鼠行为特征;各组小鼠分别于注射后12小时、24小时摘眼球采血并处死取睾丸,通过ELISA法检测小鼠血清中睾酮的浓度;通过实时荧光定量PCR(q PCR)和蛋白印迹法(Western-blot)检测小鼠睾丸中NMB、NMBR、St AR、CYP11A1和3β-HSD m RNA和蛋白表达量。结果表明:与12 h Control组相比,12 h NMB组小鼠行为无明显异常,体重增量、总摄食量略有减少,料重比略有增高,但差异不显著(P>0.05);NMB、NMBR、St AR、CYP11A1和3β-HSD m RNA及蛋白表达量均差异不显著(P>0.05);血清中睾酮浓度差异不显著(P>0.05)。与24 h Control组相比,24 h NMB组小鼠被毛粗乱、脾气暴躁,体重增量显著减少(P<0.05);总摄食量减少,料重比略有增高,但均差异不显著(P>0.05);NMB m RNA和蛋白表达量极显著降低(P<0.01),NMBR、St AR、CYP11A1和3β-HSD m RNA及蛋白表达量均差异不显著(P>0.05);血清中睾酮浓度差异不显著(P>0.05)。与12 h NMB组相比,24 h NMB组NMBR m RNA表达量极显著降低(P<0.01),24 h NMB组NMBR蛋白表达量显著增加(P<0.05)。说明小鼠腹腔注射NMB能够抑制小鼠摄食,但对小鼠睾丸中睾酮的合成和分泌影响不大。展开更多
In this study,the ZIF-8 membrane(ZIF-8/PP-g-MAH)is prepared by in situ synthesis of ZIF-8 on irradiation-pretreated polymer substrates to improve the uranium adsorption performance and address the recycling problems o...In this study,the ZIF-8 membrane(ZIF-8/PP-g-MAH)is prepared by in situ synthesis of ZIF-8 on irradiation-pretreated polymer substrates to improve the uranium adsorption performance and address the recycling problems of ZIF-8 powder.The effects of pH,contact time,and uranium concentration on the adsorption of ZIF-8/PP-g-MAH were investigated.Adsorption isotherm and kinetics analysis show that ZIF-8/PP-g-MAH has a high adsorption capacity of 478.5 mg/g,which is 1.26 times higher than that of ZIF-8,and a rapid adsorption equilibrium of 120 min,which is shortened to one-third of that required for ZIF-8(360 min).The adsorption process of ZIF-8/PP-g-MAH is consistent with that of the Langmuir isotherm and pseudo-second-order dynamic model.ZIF-8/PP-g-MAH also exhibits good selectivity for uranium in simulated seawater.The high adsorption performance of ZIF-8/PP-g-MAH is attributed to its membrane structure,which improves the utilization of coordination sites,including Zn-OH,C-N,and C=N.This study provides an efficient adsorption material for rapid uranium extraction,thus promoting the development of uranium extraction technologies.展开更多
The beginning of October is the high occurrence period of straw burning in North China and Jianghuai region. Heavy pollution weather caused by straw burning often takes place in recent two years,and thus human factor ...The beginning of October is the high occurrence period of straw burning in North China and Jianghuai region. Heavy pollution weather caused by straw burning often takes place in recent two years,and thus human factor increases the unpredictability of the air quality forecast. In the early autumn of 2015,a continuous fog-haze weather and heavy air pollution event occurred in North China and Jianghuai region due to the straw burning. In this paper,the effect of straw burning on air quality under the same adverse diffusive meteorological conditions has been analyzed by using the ground and upper air meteorological observation data and air quality monitoring data of Environmental Protection Agency in recent two years. The comparison results show that the basic conditions of heavy pollution are weak wind and the unfavorable diffusion situation. In contrast,relative humidity is the key factor,only 40% of relative humidity during the straw burning can reach heavy pollution level during no straw burning.展开更多
文摘为了研究注射外源性神经介素B(NMB)对小鼠睾丸中NMB及其受体和睾酮合成关键酶(St AR、CYP11A1和3β-HSD)表达及血清中睾酮浓度的影响,试验将40只雄性ICR小鼠随机分成12 h对照组(12 h Control组)、12 h试验组(12 h NMB组)、24 h对照组(24 h Control组)和24 h试验组(24 h NMB组),每组10只,2个对照组和2个试验组分别按体重10μL/g腹腔注射生理盐水和10 nmol/L NMB溶液;称量注射前后小鼠体重,计算饲料用量,并观察小鼠行为特征;各组小鼠分别于注射后12小时、24小时摘眼球采血并处死取睾丸,通过ELISA法检测小鼠血清中睾酮的浓度;通过实时荧光定量PCR(q PCR)和蛋白印迹法(Western-blot)检测小鼠睾丸中NMB、NMBR、St AR、CYP11A1和3β-HSD m RNA和蛋白表达量。结果表明:与12 h Control组相比,12 h NMB组小鼠行为无明显异常,体重增量、总摄食量略有减少,料重比略有增高,但差异不显著(P>0.05);NMB、NMBR、St AR、CYP11A1和3β-HSD m RNA及蛋白表达量均差异不显著(P>0.05);血清中睾酮浓度差异不显著(P>0.05)。与24 h Control组相比,24 h NMB组小鼠被毛粗乱、脾气暴躁,体重增量显著减少(P<0.05);总摄食量减少,料重比略有增高,但均差异不显著(P>0.05);NMB m RNA和蛋白表达量极显著降低(P<0.01),NMBR、St AR、CYP11A1和3β-HSD m RNA及蛋白表达量均差异不显著(P>0.05);血清中睾酮浓度差异不显著(P>0.05)。与12 h NMB组相比,24 h NMB组NMBR m RNA表达量极显著降低(P<0.01),24 h NMB组NMBR蛋白表达量显著增加(P<0.05)。说明小鼠腹腔注射NMB能够抑制小鼠摄食,但对小鼠睾丸中睾酮的合成和分泌影响不大。
文摘In this study,the ZIF-8 membrane(ZIF-8/PP-g-MAH)is prepared by in situ synthesis of ZIF-8 on irradiation-pretreated polymer substrates to improve the uranium adsorption performance and address the recycling problems of ZIF-8 powder.The effects of pH,contact time,and uranium concentration on the adsorption of ZIF-8/PP-g-MAH were investigated.Adsorption isotherm and kinetics analysis show that ZIF-8/PP-g-MAH has a high adsorption capacity of 478.5 mg/g,which is 1.26 times higher than that of ZIF-8,and a rapid adsorption equilibrium of 120 min,which is shortened to one-third of that required for ZIF-8(360 min).The adsorption process of ZIF-8/PP-g-MAH is consistent with that of the Langmuir isotherm and pseudo-second-order dynamic model.ZIF-8/PP-g-MAH also exhibits good selectivity for uranium in simulated seawater.The high adsorption performance of ZIF-8/PP-g-MAH is attributed to its membrane structure,which improves the utilization of coordination sites,including Zn-OH,C-N,and C=N.This study provides an efficient adsorption material for rapid uranium extraction,thus promoting the development of uranium extraction technologies.
基金Supported by Scientific Technology Item of State Grid Corporation of China(52010115002P)
文摘The beginning of October is the high occurrence period of straw burning in North China and Jianghuai region. Heavy pollution weather caused by straw burning often takes place in recent two years,and thus human factor increases the unpredictability of the air quality forecast. In the early autumn of 2015,a continuous fog-haze weather and heavy air pollution event occurred in North China and Jianghuai region due to the straw burning. In this paper,the effect of straw burning on air quality under the same adverse diffusive meteorological conditions has been analyzed by using the ground and upper air meteorological observation data and air quality monitoring data of Environmental Protection Agency in recent two years. The comparison results show that the basic conditions of heavy pollution are weak wind and the unfavorable diffusion situation. In contrast,relative humidity is the key factor,only 40% of relative humidity during the straw burning can reach heavy pollution level during no straw burning.
