Severe soil erosion and drought are the two main factors affecting the ecological security of the Loess Plateau,China.Investigating the influence of drought on soil conservation service is of great importance to regio...Severe soil erosion and drought are the two main factors affecting the ecological security of the Loess Plateau,China.Investigating the influence of drought on soil conservation service is of great importance to regional environmental protection and sustainable development.However,there is little research on the coupling relationship between them.In this study,focusing on the Jinghe River Basin,China as a case study,we conducted a quantitative evaluation on meteorological,hydrological,and agricultural droughts(represented by the Standardized Precipitation Index(SPI),Standardized Runoff Index(SRI),and Standardized Soil Moisture Index(SSMI),respectively)using the Variable Infiltration Capacity(VIC)model,and quantified the soil conservation service using the Revised Universal Soil Loss Equation(RUSLE)in the historical period(2000-2019)and future period(2026-2060)under two Representative Concentration Pathways(RCPs)(RCP4.5 and RCP8.5).We further examined the influence of the three types of drought on soil conservation service at annual and seasonal scales.The NASA Earth Exchange Global Daily Downscaled Projections(NEX-GDDP)dataset was used to predict and model the hydrometeorological elements in the future period under the RCP4.5 and RCP8.5 scenarios.The results showed that in the historical period,annual-scale meteorological drought exhibited the highest intensity,while seasonal-scale drought was generally weakest in autumn and most severe in summer.Drought intensity of all three types of drought will increase over the next 40 years,with a greater increase under the RCP4.5 scenario than under the RCP8.5 scenario.Furthermore,the intra-annual variation in the drought intensity of the three types of drought becomes smaller under the two future scenarios relative to the historical period(2000-2019).Soil conservation service exhibits a distribution pattern characterized by high levels in the southwest and southeast and lower levels in the north,and this pattern has remained consistent both in the historical and future periods.Over the past 20 years,the intra-annual variation indicated peak soil conservation service in summer and lowest level in winter;the total soil conservation of the Jinghe River Basin displayed an upward trend,with the total soil conservation in 2019 being 1.14 times higher than that in 2000.The most substantial impact on soil conservation service arises from annual-scale meteorological drought,which remains consistent both in the historical and future periods.Additionally,at the seasonal scale,meteorological drought exerts the highest influence on soil conservation service in winter and autumn,particularly under the RCP4.5 and RCP8.5 scenarios.Compared to the historical period,the soil conservation service in the Jinghe River Basin will be significantly more affected by drought in the future period in terms of both the affected area and the magnitude of impact.This study conducted beneficial attempts to evaluate and predict the dynamic characteristics of watershed drought and soil conservation service,as well as the response of soil conservation service to different types of drought.Clarifying the interrelationship between the two is the foundation for achieving sustainable development in a relatively arid and severely eroded area such as the Jinghe River Basin.展开更多
The SMILE(Solar wind Magnetosphere Ionosphere Link Explorer)mission is a joint space science mission between the Chinese Academy of Sciences(CAS)and the European Space Agency(ESA),aiming to understand the interaction ...The SMILE(Solar wind Magnetosphere Ionosphere Link Explorer)mission is a joint space science mission between the Chinese Academy of Sciences(CAS)and the European Space Agency(ESA),aiming to understand the interaction of the solar wind with the Earth’s magnetosphere in a global manner.As of May 2024,the SMILE mission is in phase-D with an expected launch date of September 2025.This report summarizes developments in the mission during the past two years.展开更多
Objective: To explore the protective effect of camellia oil against H2O2-induced oxidative stress injury in rat H9C2 cardiomyocytes. Methods: CCK8 method was used to detect the cell survival rate of H9C2 cardiomyocyte...Objective: To explore the protective effect of camellia oil against H2O2-induced oxidative stress injury in rat H9C2 cardiomyocytes. Methods: CCK8 method was used to detect the cell survival rate of H9C2 cardiomyocytes treated with different concentrations of H2O2. Normal cultured cells were used as the blank control group, and the cells were treated with 200 μmol/L H2O2 for 24 h. An oxidative stress injury model was constructed as the model group. The cells were pretreated with 1%, 0.1% and 0.01% camellia oil for 24 h, and then H2O2 was added for 24 h as the experimental group. The β-galactosidase senescence staining assay, mitochondrial membrane potential assay, EdU cell proliferation staining assay and scratch assay were used to observe the changes of cell senescence, mitochondrial membrane potential, proliferation, apoptosis and migration in each group. The superoxide dismutase (SOD) activity, lactate dehydrogenase (LDH) activity, and malondialdehyde (MDA) content of the cells in each group were detected by using the kit. Results: The cell viability of H9C2 cardiomyocytes treated with different concentrations of H2O2 was inhibited and positively correlated with the concentration of H2O2 (P<0.01). Compared with the blank control group, the positive rate of cell senescence, MDA content and LDH activity increased in the H2O2 model group (P<0.01);mitochondrial membrane potential, cellular value-added rate, migration rate and SOD activity decreased (P<0.01). Compared with the H2O2 model group, the positive rate of cellular senescence (P<0.01 or P<0.05), MDA content and LDH activity decreased (P< 0.01 or P<0.05);mitochondrial membrane potential increased, cell proliferation rate and migration rate increased (P<0.01 or P<0.05) in the experimental group. Conclusion: Camellia oil can significantly inhibit oxidative stress injury in H9C2 cells and exert cardiomyocyte protective effects.展开更多
The forest-based wellness industry,as a rapidly growing sector that integrates various business forms with extensive coverage and an extended industrial chain,is undergoing rapid development due to the increasing agin...The forest-based wellness industry,as a rapidly growing sector that integrates various business forms with extensive coverage and an extended industrial chain,is undergoing rapid development due to the increasing aging population and people's suboptimal health conditions.As a forerunner in developing the forest-based wellness industry,Sichuan province is known for its early development,proactive efforts,diverse models,and significant impact in this industry.It has achieved certain milestones in terms of top-level design,pilot demonstration,standardized guidance,and public awareness campaigns to promote the development of this industry.Therefore,this paper utilizes Sichuan as a case study to systematically summarize and analyze the key practices made by the province in promoting the rapid development of the industry by investigating the development trajectory of the forest-based wellness industry.Additionally,it examines the development trends of this industry from the perspectives of supply,demand,and consumption.Finally,this paper proposes several measures to facilitate the high-quality development of the forest-based wellness industry.These measures encompass nurturing specialized talent in forest-based wellness,enhancing market players'capabilities in this domain,conducting extensive research on technologies that promote this industry,actively seeking support from relevant policies,and promoting integrated development across diverse sectors.展开更多
目的预测疥螨猪变种转录组中过敏原蛋白,对Sar s 14.3过敏原蛋白进行生物特性分析、原核表达并建立间接ELISA诊断方法。方法利用转录组测序(RNA-Seq)及BLAST、ProtParam、I-TASSER和COMPARE等软件和数据库对疥螨猪变种转录组中的过敏原...目的预测疥螨猪变种转录组中过敏原蛋白,对Sar s 14.3过敏原蛋白进行生物特性分析、原核表达并建立间接ELISA诊断方法。方法利用转录组测序(RNA-Seq)及BLAST、ProtParam、I-TASSER和COMPARE等软件和数据库对疥螨猪变种转录组中的过敏原组分进行预测并对疥螨Sar s 14.3过敏原蛋白序列进行理化性质分析、结构分析、同源性分析和间接ELISA诊断方法的建立。结果在疥螨猪变种的18980个转录本中共预测出390条过敏原序列,且随机匹配可能性值(E-value)为0的序列有28条。Sar s 14.3过敏原蛋白的理论相对分子质量为38 kDa,蛋白较稳定,为亲水性蛋白;二级结构以无规卷曲和β-折叠为主,其氨基酸序列与疥螨其他变种主要过敏原组分14蛋白序列相似性不低于99.76%,而与尘螨属螨虫的相似性最高为73.08%,与现行的形态学分类系统一致。所建立的间接ELISA诊断方法的最佳包被抗原浓度为2μg/mL,血清最佳稀释倍数是1∶400。结论本试验成功预测了疥螨猪变种转录组中的过敏原序列并克隆、表达了疥螨猪变种Sar s 14.3蛋白,初步预测了其分子特征,并以该过敏原蛋白为基础成功建立了一种快速的间接ELISA检测方法,可用于疥螨病的初步诊断和流行病学调查。展开更多
针对顾客产品偏好快速变化对企业分析和预测顾客偏好能力的要求,提出一种面向产品改进的顾客偏好分析与预测方法,首先构建长短期记忆网络模型,预测产品设计迭代期间的情感值和重要度,并计算预测准确度;然后通过基于产品特征情感变化模...针对顾客产品偏好快速变化对企业分析和预测顾客偏好能力的要求,提出一种面向产品改进的顾客偏好分析与预测方法,首先构建长短期记忆网络模型,预测产品设计迭代期间的情感值和重要度,并计算预测准确度;然后通过基于产品特征情感变化模式的产品设计改进模型判断各个特征的变化模式,明确待改进的产品特征及改进优先级;最后以DJI Mini 2无人机的在线评论为例验证了方法的有效性。展开更多
基金supported by the National Natural Science Foundation of China(42071285,42371297)the Key R&D Program Projects in Shaanxi Province of China(2022SF-382)the Fundamental Research Funds for the Central Universities(GK202302002).
文摘Severe soil erosion and drought are the two main factors affecting the ecological security of the Loess Plateau,China.Investigating the influence of drought on soil conservation service is of great importance to regional environmental protection and sustainable development.However,there is little research on the coupling relationship between them.In this study,focusing on the Jinghe River Basin,China as a case study,we conducted a quantitative evaluation on meteorological,hydrological,and agricultural droughts(represented by the Standardized Precipitation Index(SPI),Standardized Runoff Index(SRI),and Standardized Soil Moisture Index(SSMI),respectively)using the Variable Infiltration Capacity(VIC)model,and quantified the soil conservation service using the Revised Universal Soil Loss Equation(RUSLE)in the historical period(2000-2019)and future period(2026-2060)under two Representative Concentration Pathways(RCPs)(RCP4.5 and RCP8.5).We further examined the influence of the three types of drought on soil conservation service at annual and seasonal scales.The NASA Earth Exchange Global Daily Downscaled Projections(NEX-GDDP)dataset was used to predict and model the hydrometeorological elements in the future period under the RCP4.5 and RCP8.5 scenarios.The results showed that in the historical period,annual-scale meteorological drought exhibited the highest intensity,while seasonal-scale drought was generally weakest in autumn and most severe in summer.Drought intensity of all three types of drought will increase over the next 40 years,with a greater increase under the RCP4.5 scenario than under the RCP8.5 scenario.Furthermore,the intra-annual variation in the drought intensity of the three types of drought becomes smaller under the two future scenarios relative to the historical period(2000-2019).Soil conservation service exhibits a distribution pattern characterized by high levels in the southwest and southeast and lower levels in the north,and this pattern has remained consistent both in the historical and future periods.Over the past 20 years,the intra-annual variation indicated peak soil conservation service in summer and lowest level in winter;the total soil conservation of the Jinghe River Basin displayed an upward trend,with the total soil conservation in 2019 being 1.14 times higher than that in 2000.The most substantial impact on soil conservation service arises from annual-scale meteorological drought,which remains consistent both in the historical and future periods.Additionally,at the seasonal scale,meteorological drought exerts the highest influence on soil conservation service in winter and autumn,particularly under the RCP4.5 and RCP8.5 scenarios.Compared to the historical period,the soil conservation service in the Jinghe River Basin will be significantly more affected by drought in the future period in terms of both the affected area and the magnitude of impact.This study conducted beneficial attempts to evaluate and predict the dynamic characteristics of watershed drought and soil conservation service,as well as the response of soil conservation service to different types of drought.Clarifying the interrelationship between the two is the foundation for achieving sustainable development in a relatively arid and severely eroded area such as the Jinghe River Basin.
基金Founded by the Strategic Priority Research Program on Space Science,the Chinese Academy of Sciences(XDA15350000)。
文摘The SMILE(Solar wind Magnetosphere Ionosphere Link Explorer)mission is a joint space science mission between the Chinese Academy of Sciences(CAS)and the European Space Agency(ESA),aiming to understand the interaction of the solar wind with the Earth’s magnetosphere in a global manner.As of May 2024,the SMILE mission is in phase-D with an expected launch date of September 2025.This report summarizes developments in the mission during the past two years.
基金National Natural Science Foundation of China(No.82160597)Guangxi Natural Science Foundation Project(No.2020GXNSFAA159148)。
文摘Objective: To explore the protective effect of camellia oil against H2O2-induced oxidative stress injury in rat H9C2 cardiomyocytes. Methods: CCK8 method was used to detect the cell survival rate of H9C2 cardiomyocytes treated with different concentrations of H2O2. Normal cultured cells were used as the blank control group, and the cells were treated with 200 μmol/L H2O2 for 24 h. An oxidative stress injury model was constructed as the model group. The cells were pretreated with 1%, 0.1% and 0.01% camellia oil for 24 h, and then H2O2 was added for 24 h as the experimental group. The β-galactosidase senescence staining assay, mitochondrial membrane potential assay, EdU cell proliferation staining assay and scratch assay were used to observe the changes of cell senescence, mitochondrial membrane potential, proliferation, apoptosis and migration in each group. The superoxide dismutase (SOD) activity, lactate dehydrogenase (LDH) activity, and malondialdehyde (MDA) content of the cells in each group were detected by using the kit. Results: The cell viability of H9C2 cardiomyocytes treated with different concentrations of H2O2 was inhibited and positively correlated with the concentration of H2O2 (P<0.01). Compared with the blank control group, the positive rate of cell senescence, MDA content and LDH activity increased in the H2O2 model group (P<0.01);mitochondrial membrane potential, cellular value-added rate, migration rate and SOD activity decreased (P<0.01). Compared with the H2O2 model group, the positive rate of cellular senescence (P<0.01 or P<0.05), MDA content and LDH activity decreased (P< 0.01 or P<0.05);mitochondrial membrane potential increased, cell proliferation rate and migration rate increased (P<0.01 or P<0.05) in the experimental group. Conclusion: Camellia oil can significantly inhibit oxidative stress injury in H9C2 cells and exert cardiomyocyte protective effects.
基金supported by the major project of Sichuan Social Science Planning Project“Study on the Realization Path of Promoting Common Prosperity in Sichuan”。
文摘The forest-based wellness industry,as a rapidly growing sector that integrates various business forms with extensive coverage and an extended industrial chain,is undergoing rapid development due to the increasing aging population and people's suboptimal health conditions.As a forerunner in developing the forest-based wellness industry,Sichuan province is known for its early development,proactive efforts,diverse models,and significant impact in this industry.It has achieved certain milestones in terms of top-level design,pilot demonstration,standardized guidance,and public awareness campaigns to promote the development of this industry.Therefore,this paper utilizes Sichuan as a case study to systematically summarize and analyze the key practices made by the province in promoting the rapid development of the industry by investigating the development trajectory of the forest-based wellness industry.Additionally,it examines the development trends of this industry from the perspectives of supply,demand,and consumption.Finally,this paper proposes several measures to facilitate the high-quality development of the forest-based wellness industry.These measures encompass nurturing specialized talent in forest-based wellness,enhancing market players'capabilities in this domain,conducting extensive research on technologies that promote this industry,actively seeking support from relevant policies,and promoting integrated development across diverse sectors.
文摘目的预测疥螨猪变种转录组中过敏原蛋白,对Sar s 14.3过敏原蛋白进行生物特性分析、原核表达并建立间接ELISA诊断方法。方法利用转录组测序(RNA-Seq)及BLAST、ProtParam、I-TASSER和COMPARE等软件和数据库对疥螨猪变种转录组中的过敏原组分进行预测并对疥螨Sar s 14.3过敏原蛋白序列进行理化性质分析、结构分析、同源性分析和间接ELISA诊断方法的建立。结果在疥螨猪变种的18980个转录本中共预测出390条过敏原序列,且随机匹配可能性值(E-value)为0的序列有28条。Sar s 14.3过敏原蛋白的理论相对分子质量为38 kDa,蛋白较稳定,为亲水性蛋白;二级结构以无规卷曲和β-折叠为主,其氨基酸序列与疥螨其他变种主要过敏原组分14蛋白序列相似性不低于99.76%,而与尘螨属螨虫的相似性最高为73.08%,与现行的形态学分类系统一致。所建立的间接ELISA诊断方法的最佳包被抗原浓度为2μg/mL,血清最佳稀释倍数是1∶400。结论本试验成功预测了疥螨猪变种转录组中的过敏原序列并克隆、表达了疥螨猪变种Sar s 14.3蛋白,初步预测了其分子特征,并以该过敏原蛋白为基础成功建立了一种快速的间接ELISA检测方法,可用于疥螨病的初步诊断和流行病学调查。
基金supported by grants from the Youth Innovative Talents Program and Characteristic Innovative Talents Program of Regular Institutions of Higher Education of Guangdong Province(2018KQNCX212,2020KTSCX101)the Scientific Research Project of Traditional Chinese Medicine Bureau of Guangdong Province(20222122)+3 种基金the Excellent Youth Science Research Program of the Education Department of Hunan Province(14B183)Bureau of Education of Guangzhou Municipality(202032801)the Plan on Enhancing Scientific Research in Guangzhou Medical Universitythe Open Research Funds(2021)and the Funds of Selected Project(2022)from GMU-GIBH Joint School of Life Sciences,Guangzhou Medical University。
文摘目的oxLDL可上调Plin2的表达,进而促进泡沫细胞的形成,LOX1是oxLDL的受体。本文探讨Plin2与LOX1在动脉粥样硬化发生发展过程中的关系。方法从GEO数据库中下载GSE43292,分析Plin2、LOX1的表达及Plin2、LOX1与NF-κB信号通路的相关性。采用oxLDL处理的RAW264.7细胞作为动脉粥样硬化的细胞模型进行研究,蛋白质免疫印迹法检测细胞中Plin2、LOX1和p-p65的表达,荧光中性脂质染料BODIPY 493/503染色法检测细胞内脂滴。结果通过分析GSE43292数据发现,Plin2、LOX1在颈动脉粥样硬化斑块中的表达显著高于颈动脉邻近组织。oxLDL处理RAW264.7细胞24 h后,Plin2与LOX1的表达、细胞内脂滴明显增加。过表达Plin2的细胞中LOX1表达升高;当用oxLDL孵育过表达Plin2的细胞后,LOX1的水平升高更为显著;但在没有oxLDL处理的情况下,敲减Plin2对细胞内LOX1的表达没有影响。基因集富集分析(gene set enrichment analysis,GSEA)结果显示,在动脉粥样硬化中,Plin2和LOX1的表达与NF-κB的活化呈正相关。此外,尽管采用oxLDL处理细胞,NF-κB抑制剂JSH-23预处理仍可显著降低Plin2与LOX1的表达、细胞内的脂质积聚,过表达Plin2后,JSH-23亦能显著抑制oxLDL孵育的细胞中Plin2和LOX1的表达。结论Plin2可通过上调LOX1的表达促进细胞内脂质积聚,参与动脉粥样硬化,这一过程至少部分是通过激活NF-κB通路实现的。
文摘针对顾客产品偏好快速变化对企业分析和预测顾客偏好能力的要求,提出一种面向产品改进的顾客偏好分析与预测方法,首先构建长短期记忆网络模型,预测产品设计迭代期间的情感值和重要度,并计算预测准确度;然后通过基于产品特征情感变化模式的产品设计改进模型判断各个特征的变化模式,明确待改进的产品特征及改进优先级;最后以DJI Mini 2无人机的在线评论为例验证了方法的有效性。