The predictive value of serum S1P and STIM1 levels after PCI for in-stent restenosis and their correlation with angiogenesis and inflammatory response

Objective:To study the predictive value of serum S1P and STIM1 levels after percutaneous coronary intervention (PCI) for in-stent restenosis and their correlation with angiogenesis and inflammatory response.Methods:130 patients who received PCI in our hospital between June 2013 and December 2016 were selected and divided into restenosis group and non-restenosis group according to the coronary angiography results 6-24 months after PCI. The serum levels of S1P, STIM1, angiogenesis molecules and inflammation molecules were detected 24 hours after PCI.Results:Serum S1P, NO, VEGF, Angptl2 and Angptl4 levels of restenosis group were significantly lower than those of non-restenosis group while STIM1, IFN-γ, IL-18, VCAM-1, P-selectin and L-selectin levels were significantly higher than those of non-restenosis group;serum NO, VEGF, Angptl2 and Angptl4 levels of restenosis group with lower STIM1 were significantly higher those of restenosis group with normal STIM1;serum IFN-γ, IL-18, VCAM-1, P-selectin and L-selectin levels of restenosis group with lower S1P were significantly higher than those of restenosis group with normal S1P.Conclusion:The decreased serum S1P and increased STIM1 after PCI have prediction value for in-stent restenosis and are closely related to the angiogenesis disorder and inflammation activation.

Correlation of serum miR-148b expression with myocardial injury and myocardial fibrosis in patients with myocardial infarction

Objective:To study the correlation of serum miR-148b expression with myocardial injury and myocardial fibrosis in patients with myocardial infarction.Methods:A total of 130 patients who were diagnosed with acute myocardial infarction and 100 healthy subjects who received physical examination in Hanzhong Central Hospital between March 2013 and October 2016 were selected and enrolled in AMI group and control group respectively. Serum was collected, fluorescent quantitative PCR kit was used to detect miR-148b expression, and enzyme-linked immunosorbent assay kit was used to detect the contents of myocardial injury markers and myocardial fibrosis markers.Results:Serum miR-148b expression as well as CK-MB, cTnT, H-FABP, PICP, PIIINP, CTX-I, TGF-β1 and GDF-15 levels in AMI group was significantly higher than those in control group;serum CK-MB, cTnT, H-FABP, PICP, PIIINP, CTX-I, TGF-β1 and GDF-15 levels in AMI patients with high miR-148b expression were significantly higher than those in AMI patients with low miR-148b expression.Conclusion: Highly expressed miR-148b in serum of patients with myocardial infarction can promote myocardial injury and myocardial fibrosis.