Preliminary study on the protective effect of electroacupuncture Neiguan acupoint pretreatment on rats with myocardial ischemia-reperfusion injury:role of the miR-214-3p/NCX1 axis

Background:Ischemia-reperfusion can worsen myocardial damage and increase the risk of death.Studies have revealed that ischemic preconditioning provides the best endogenous protection against myocardial ischemia-reperfusion injury(MIRI),and the principle of electroacupuncture(EA)preconditioning is comparable to that of myocardial ischemic preconditioning adaption.Our earlier research demonstrated that EA pretreatment inhibits the expression of calmodulin-dependent protein kinase IIδ(CaMKIIδ),sodium/calcium exchanger 1(NCX1),and cyclophilin D,hence providing protection against MIRI.However,the exact mechanism is still unknown.The expression of NCX1 mRNA is directly regulated by microRNA-214(miR-214).Moreover,it suppresses the levels of CaMKIIδand cyclophilin D.Whether these variables contribute to EA preconditioning to improve MIRI needs to be investigated,though.This study aimed to preliminarily determine whether EA pretreatment ameliorates MIRI by modulating the miR-214-3p/NCX1 axis.Methods:We used a rat MIRI model to investigate the effect of EA pretreatment on MIRI and the expression of miR-214-3p.In addition,adenovirus injection inhibited miR-214-3p expression in the rat MIRI model,and the influence of EA pretreatment towards MIRI was observed in the context of blocked miR-214-3p expression.Both the myocardial histological abnormalities and the alterations in the ST segment of the rat electrocardiogram were analyzed.NCX1 mRNA,cyclophilin D,and CaMKIIδexpression levels were also analyzed.Results:EA pretreatment improved MIRI.In rats with MIRI,EA administration increased miR-214-3p expression while decreasing NCX1 mRNA,cyclophilin D,and CaMKIIδproteins in cardiac tissues.The beneficial effect of EA pretreatment against MIRI was reversed,coupled with elevated levels of NCX1 mRNA,cyclophilin D,and CaMKIIδprotein expression,when an adenovirus injection disrupted the expression of miR-214-3p.Conclusions:Our findings preliminarily show that EA pretreatment inhibits the expression of NCX1 mRNA,cyclophilin D,and CaMKIIδproteins via miR-214-3p,hence exerting MIRI protection.

Isolation and Purification of a New Bacillus Subtilis Strain from Deer Dung with Anti-microbial and Anti-cancer Activities

Objective Bacillus strains are well known for their natural bioactive products that have antimicrobial and/or anti-cancer activities.Many of Bacillus’structurally unique metabolites can combat human diseases,including cancers.However,because Bacillus’metabolites are so abundant,few have been studied extensively enough to fully characterize their chemical constitutions and biological functions.Methods In this study,we focused on the isolation and purification of a new Bacillus strain,and determined the effects of its metabolites on bacteria and cancer cells.Our study focused on a new strain of Bacillus isolated from deer dung.Based on BLAST results,this isolate belongs to Bacillus subtilis,and therefore we named the strain Bacillus subtilis NC16.Congo red assay was used to test the cellulase activity.The inhibition zone was measured to test the antimicrobial activity.CCK-8,wound healing and flow cytometry were used to test the anti-cancer activity.Results Metabolites from Bacillus subtilis NC16 have both antimicrobial and anti-cancer activities.They can both suppress the growth of Trichoderma vride and Staphylococcus aureus,and inhibit the proliferation and promote the apoptosis of non-small cell lung cancer cell lines.Conclusion Our results suggest that Bacillus subtilis NC16 can not only degrade cellulose,but its metabolites may be sources of antibiotics and anti-cancer drugs.

Control effect of structured skin care plan of integrated Chinese and Western medicine in elderly patients with incontinence-associated dermatitis

Objective:The objective of this study is to explore the clinical effects of structured skin care plan of integrated Chinese and Western medicine in intervening elderly patients with incontinence-associated dermatitis(IAD).Materials and Methods:Totally,66 elderly patients with IAD were randomly divided into the experiment group(32 cases)and control group(34 cases).The control group was given routine nursing care,while the experiment group was given a structured skin care plan.The observational course was 2 weeks.The treatment efficiency and healing time were compared between the two groups.Results:After 2-week intervention,the total effective rate of the experiment group was higher than that of the control group(97.1%vs.78.1%,X^(2)=3.913,P=0.048).The skin assessment tool score of the experiment group was lower than that of the control group(0.56±1.58 vs.1.75±2.46,Z=−−2.401,P=0.016).The healing time of the experiment group was shorter than that of the control group(7.29±4.76 days vs.10.69±6.36 days,Z=−2.280,P=0.026).Conclusion:The structured skin care plan of integrated Chinese and Western medicine showed a good effect in elderly IAD patients,and provided a reference for clinical treatment and care of elderly patients with IAD.

Deformation mechanism analysis of roll forming for Q&P980 steel based on finite element simulation

Roll forming is a sheet metal forming process,which can form the profiles gradually to improve the formability of Q&P980 steel.The plastic deformation mechanism of roll forming was expounded by analysing the stress and strain distribution at the comer of a hat-type profile when the Q&P980 steel sheet passed through a series of continuous stands.And the plastic deformation mainly accumulated when the sheet metal was not in contact with the rolls.A simple mathematical model was derived by considering the longitudinal bending strain and the geometrical relationships of forming parameters,to analyse the longitudinal strain development in the deformation process.In addition,the roll forming experiments on hat-type profile parts of Q&P980 steel were carried out,and the theoretical analysis and simulation results are consistent with the experimental results.