BACKGROUND Studies suggested that remote ischemic preconditioning(RIPC)may effectively lessen the harmful effects of ischemia reperfusion injury during organ transplantation surgery.AIM To investigate the protective e...BACKGROUND Studies suggested that remote ischemic preconditioning(RIPC)may effectively lessen the harmful effects of ischemia reperfusion injury during organ transplantation surgery.AIM To investigate the protective effects of RIPC on living liver donors and recipients following pediatric liver transplantation.METHODS From January 2016 to January 2019 at Renji Hospital Affiliated with Shanghai Jiao Tong University School of Medicine,208 donors were recruited and randomly assigned to four groups:S-RIPC group(no intervention;n=55),D-RIPC group(donors received RIPC;n=51),R-RIPC group(recipients received RIPC,n=51)and DR-RIPC group(both donors and recipients received RIPC;n=51).We primarily evaluated postoperative liver function among donors and recipients and incidences of early allograft dysfunction,primary nonfunction and postoperative complications among recipients.RESULTS RIPC did not significantly improve alanine transaminase and aspartate aminotransferase levels among donors and recipients or decrease the incidences of early allograft dysfunction,primary nonfunction,and postoperative complications among recipients.Limited protective effects were observed,including a lower creatinine level in the D-RIPC group than in the S-RIPC group on postoperative day 0(P<0.05).However,no significant improvements were found in donors who received RIPC.Furthermore,RIPC had no effects on the overall survival of recipients.CONCLUSION The protective effects of RIPC were limited for recipients who received living liver transplantation,and no significant improvement of the prognosis was observed in recipients.展开更多
OBJECTIVE To investigate the in vitro lethal effect of photo- dynamic therapy (PDT) using the photosensitizer hematoporphyrin on the human pancreatic cancer cell line Panc-1, the major influencing factors and the me...OBJECTIVE To investigate the in vitro lethal effect of photo- dynamic therapy (PDT) using the photosensitizer hematoporphyrin on the human pancreatic cancer cell line Panc-1, the major influencing factors and the mechanisms of treatment. METHODS Three factors--the time needed for photosensitizer and cell incubation, the photosensitizer concentration (PhoC) and the exposure dose (ExpD)--were examined with different levels of these factors. Optical density (OD) was used as a measure of CCK-8 in the experiment, and was converted to the rate of cell survival. The separate effect of each factor on the photodynamic action was studied, and the interactions were investigated. The effects of different incubation times and PhoC levels on the fluorescence intensity (FI) of the intracellular photosensitizer were determined, and the mechanisms of these factors leading to the therapeutic effects of PDT discussed. RESULTS An increase in the photosensitizer and cell incubation time, an increase of PhoC, and enhancement of the ExpD, produced a corresponding decrease in the rate of Panc-1 cell survival after PDT (P 〈 0.05). PDT achieved its maximum lethal effects 16 h after starting the incubation, with a PhoC of 10 mg/L and an ExpD of 20 J/cm2; at these levels a synergistic interaction between PhoC and the ExpD occurred, decreasing the cell survival rate (P 〈 0.05). Neither simple administration of photosensitizer without ExpD (0 J/cm2) or illumination in the absence of PhoC (0 mg/L) affected the rate of cell survival (P 〉 0.05). With an increase of PhoC and lengthening of the incubation time, the FI of the intracellular photosensitizer accordingly increased (P 〈 0.05), and attained its maximum value at a PhoC of 10 mg/L and 36 h after the incubation. With an increase of PhoC, the FI of the photosensitizer, hematoporphyrin, in the solution increased progressively at first and then decreased (fluorescence quenching). CONCLUSION PDT with the photosensitizer hematoporphyrin has clear lethal effects on the human pancreatic cancer cell line Panc-1, but the presence of a photosensitizer and laser irradiation by themselves do not have independent lethal effects. The three influencing factors--the time for photosensitizer and cell incuba- tion, PhoC and ExpD--correlate positively with the PDT response, within certain limits. Beyond these limits, the PDT response does not significantly increase. The main mechanism of the PDT response lies in the effect of these factors on the level of the intracellular photosensitizer and the fluorescence quenching of the photosensitizer. A synergistic effect exists between PhoC and ExpD.展开更多
This review focuses on the mechanisms involved in hepatic ischemia-reperfusion(I/R) injury and effective therapeutic treatments associated with clinical anesthesia. Although hepatocytes are the main target cells in th...This review focuses on the mechanisms involved in hepatic ischemia-reperfusion(I/R) injury and effective therapeutic treatments associated with clinical anesthesia. Although hepatocytes are the main target cells in the whole process of I/R injury, Kupffer cells, as an initiator of harmful cascades, may play a vital role by releasing some proinflammatory mediators and reactive oxygen species in the early phase of I/R injury. The subsequent activation and recruitment of neutrophils are also involved in inflammatory response and immune activation. According to the above mechanisms, a number of strategies have been put forward in some experimental and clinical studies. Most of these therapeutic treatments originated from the generation of oxygen radicals and cytokines, the infiltration of neutrophils, the impairment of microcirculation and so on. Furthermore, increasing evidence has suggested that short periods of ischemic preconditioning have protective effects against liver I/R injury. Depending on these investigations, pharmacological preconditioning and clinical anesthesia-related effective methods have been proposed. A better understanding of the present progress on experimental statistics will bring about novel therapeutic treatments for the improvement of liver surgeries and transplantation.展开更多
This paper proposes a novel method to compute the diffeomorphic registration of 3D surfaces with point and curve feature landmarks.First the surfaces are mapped to the canonical domain by a curve constrained harmonicm...This paper proposes a novel method to compute the diffeomorphic registration of 3D surfaces with point and curve feature landmarks.First the surfaces are mapped to the canonical domain by a curve constrained harmonicmap,where the landmark curves are straightened to line segments and their positions and inclining angles are determined intrinsically by the surface geometry and its curve landmarks.Then,the canonical domains are registered by aligning the corresponding point and straight line segments using the dynamic quasiconformal map(DQCM),which introduces the combinatorial diagonal switches to the quasiconformal optimization such that the resultant map is diffeomorphic.The end points of the source curve landmarks are mapped to their corresponding points on the target surface,while the interior points of the source curves can slide on the corresponding target curves,which provides more freedom for the surface registration than the point-based registration methods.Experiments on the real surfaces with point and curve landmarks demonstrate the efficiency,efficacy and robustness of the proposed method.展开更多
Background and Aims:The results of basic research implicate the vascular endothelial growth factor(VEGF)family as a potential target of hepatopulmonary syndrome(HPS).However,the negative results of anti-angiogenetic t...Background and Aims:The results of basic research implicate the vascular endothelial growth factor(VEGF)family as a potential target of hepatopulmonary syndrome(HPS).However,the negative results of anti-angiogenetic therapy in clinical studies have highlighted the need for markers for HPS.Therefore,we aimed to determine whether VEGF family members and their receptors can be potential biomarkers for HPS through clinical and experimental studies.Methods:Clinically,patients with chronic liver disease from two medical centers were enrolled and examined for HPS.Patients were divided into HPS,intrapulmonary vascular dilation[positive contrast-enhanced echocardiography(CEE)and normal oxygenation]and CEE-negative groups.Baseline information and perioperative clinical data were compared between HPS and non-HPS patients.Serum levels of VEGF family members and their receptors were measured.In parallel,HPS rats were established by common bile duct ligation.Liver,lung and serum samples were collected for the evaluation of pathophysiologic changes,as well as the expression levels of the above factors.Results:In HPS rats,all VEGF family members and their receptors underwent significant changes;however,only soluble VEGFR1(sFlt-1)and the sFlt-1/placental growth factor(PLGF)ratio were changed in almost the same manner as those in HPS patients.Furthermore,through feature selection and internal and external validation,sFlt-1 and the sFlt-1/PLGF ratio were identified as the most important variables to distinguish HPS from non-HPS patients.Conclusions:Our results from animal and human studies indicate that sFlt-1 and the sFlt-1/PLGF ratio in serum are potential markers for HPS.展开更多
The Hedgehog(Hh)signaling pathway is one o f the major regulators of embryonic development and tissue homeostasis in multicellular organisms.However,the role of this pathway in the silkworm,especially in the silkworm ...The Hedgehog(Hh)signaling pathway is one o f the major regulators of embryonic development and tissue homeostasis in multicellular organisms.However,the role of this pathway in the silkworm,especially in the silkworm midgut,remains poorly understood.Here,we report that Bombyx mori Hedgehog(BmHh)is expressed in most tissues of silkworm larvae and that its functions are well-conserved throughout evolution.We further demonstrate that the messenger RNA o f four Hh signaling components,BmHh ligand,BmPtch receptor,signal transducer BmSmo and transcription factor BmCi,are all upregulated following Escherichia coli or Bacillus thuringiensis infection,indicating the activation o f the Hh pathway.Simultaneously,midgut cell proliferation is strongly promoted.Conversely,the repression of Hh signal transduction with double-stranded RNA or cyclopamine inhibits the expression o f BmHh and BmCi and reduces cell proliferation.Overall,these findings provide new insights into the Hh signaling pathway in the silkworm,B.mori.展开更多
Combined with Fourier transform infrared spectroscopy(FTIR) analysis of sodium vanadate solution,the relationship between conductivity and structure was investigated by measuring the electric conductivity of the sol...Combined with Fourier transform infrared spectroscopy(FTIR) analysis of sodium vanadate solution,the relationship between conductivity and structure was investigated by measuring the electric conductivity of the solution under different alkali concentrations and molar ratios of NaOH to V2O5. Results suggest that the polymerization vanadium acid radical ions gradually transform into monomer with the solution diluting. When the solution is diluted to a certain extent,only the vanadium acid radical ion with V-OH chemical bond exists in the solution. At NaOH concentration of below 105.21 g·L^(-1),the vanadate anions mainly exist in the form of vanadium acid radical ion with V-OH chemical bond and the ion transference number is approximately from 0.58 to 0.82. In the medium NaOH concentration range of 105.21-117.03 g·L^(-1), the vanadate anions mostly exist in the form of vanadium acid radical ion with V-OH and V-O-V chemical bonds and the ion transference number is approximately 3.29. At NaOH concentration of above 117.03 g·L^(-1), vanadate anions exist in the form of vanadium acid radical ion with V-OH and V-O-V chemical bonds.展开更多
基金Supported by Renji Hospital Clinical Innovation Foundation,No.PYIII-17-002Outstanding Academic Leaders’Program of Health and Family Planning Commission of Shanghai,No.2017BR042+1 种基金Investigative Doctor Program(2017)of Shanghai Jiao Tong University School of MedicineJoint Project of Health and Family Planning Commission of Pudong District,No.PW2015D-3.
文摘BACKGROUND Studies suggested that remote ischemic preconditioning(RIPC)may effectively lessen the harmful effects of ischemia reperfusion injury during organ transplantation surgery.AIM To investigate the protective effects of RIPC on living liver donors and recipients following pediatric liver transplantation.METHODS From January 2016 to January 2019 at Renji Hospital Affiliated with Shanghai Jiao Tong University School of Medicine,208 donors were recruited and randomly assigned to four groups:S-RIPC group(no intervention;n=55),D-RIPC group(donors received RIPC;n=51),R-RIPC group(recipients received RIPC,n=51)and DR-RIPC group(both donors and recipients received RIPC;n=51).We primarily evaluated postoperative liver function among donors and recipients and incidences of early allograft dysfunction,primary nonfunction and postoperative complications among recipients.RESULTS RIPC did not significantly improve alanine transaminase and aspartate aminotransferase levels among donors and recipients or decrease the incidences of early allograft dysfunction,primary nonfunction,and postoperative complications among recipients.Limited protective effects were observed,including a lower creatinine level in the D-RIPC group than in the S-RIPC group on postoperative day 0(P<0.05).However,no significant improvements were found in donors who received RIPC.Furthermore,RIPC had no effects on the overall survival of recipients.CONCLUSION The protective effects of RIPC were limited for recipients who received living liver transplantation,and no significant improvement of the prognosis was observed in recipients.
基金This work was supported by grants from Guangdong Provincial Natural Science Foundation (06021369) and Guangdong Medical Research Funds (B2006043).
文摘OBJECTIVE To investigate the in vitro lethal effect of photo- dynamic therapy (PDT) using the photosensitizer hematoporphyrin on the human pancreatic cancer cell line Panc-1, the major influencing factors and the mechanisms of treatment. METHODS Three factors--the time needed for photosensitizer and cell incubation, the photosensitizer concentration (PhoC) and the exposure dose (ExpD)--were examined with different levels of these factors. Optical density (OD) was used as a measure of CCK-8 in the experiment, and was converted to the rate of cell survival. The separate effect of each factor on the photodynamic action was studied, and the interactions were investigated. The effects of different incubation times and PhoC levels on the fluorescence intensity (FI) of the intracellular photosensitizer were determined, and the mechanisms of these factors leading to the therapeutic effects of PDT discussed. RESULTS An increase in the photosensitizer and cell incubation time, an increase of PhoC, and enhancement of the ExpD, produced a corresponding decrease in the rate of Panc-1 cell survival after PDT (P 〈 0.05). PDT achieved its maximum lethal effects 16 h after starting the incubation, with a PhoC of 10 mg/L and an ExpD of 20 J/cm2; at these levels a synergistic interaction between PhoC and the ExpD occurred, decreasing the cell survival rate (P 〈 0.05). Neither simple administration of photosensitizer without ExpD (0 J/cm2) or illumination in the absence of PhoC (0 mg/L) affected the rate of cell survival (P 〉 0.05). With an increase of PhoC and lengthening of the incubation time, the FI of the intracellular photosensitizer accordingly increased (P 〈 0.05), and attained its maximum value at a PhoC of 10 mg/L and 36 h after the incubation. With an increase of PhoC, the FI of the photosensitizer, hematoporphyrin, in the solution increased progressively at first and then decreased (fluorescence quenching). CONCLUSION PDT with the photosensitizer hematoporphyrin has clear lethal effects on the human pancreatic cancer cell line Panc-1, but the presence of a photosensitizer and laser irradiation by themselves do not have independent lethal effects. The three influencing factors--the time for photosensitizer and cell incuba- tion, PhoC and ExpD--correlate positively with the PDT response, within certain limits. Beyond these limits, the PDT response does not significantly increase. The main mechanism of the PDT response lies in the effect of these factors on the level of the intracellular photosensitizer and the fluorescence quenching of the photosensitizer. A synergistic effect exists between PhoC and ExpD.
文摘This review focuses on the mechanisms involved in hepatic ischemia-reperfusion(I/R) injury and effective therapeutic treatments associated with clinical anesthesia. Although hepatocytes are the main target cells in the whole process of I/R injury, Kupffer cells, as an initiator of harmful cascades, may play a vital role by releasing some proinflammatory mediators and reactive oxygen species in the early phase of I/R injury. The subsequent activation and recruitment of neutrophils are also involved in inflammatory response and immune activation. According to the above mechanisms, a number of strategies have been put forward in some experimental and clinical studies. Most of these therapeutic treatments originated from the generation of oxygen radicals and cytokines, the infiltration of neutrophils, the impairment of microcirculation and so on. Furthermore, increasing evidence has suggested that short periods of ischemic preconditioning have protective effects against liver I/R injury. Depending on these investigations, pharmacological preconditioning and clinical anesthesia-related effective methods have been proposed. A better understanding of the present progress on experimental statistics will bring about novel therapeutic treatments for the improvement of liver surgeries and transplantation.
基金supported by the China National Natural Science Foundation(61872224,12090021).
文摘This paper proposes a novel method to compute the diffeomorphic registration of 3D surfaces with point and curve feature landmarks.First the surfaces are mapped to the canonical domain by a curve constrained harmonicmap,where the landmark curves are straightened to line segments and their positions and inclining angles are determined intrinsically by the surface geometry and its curve landmarks.Then,the canonical domains are registered by aligning the corresponding point and straight line segments using the dynamic quasiconformal map(DQCM),which introduces the combinatorial diagonal switches to the quasiconformal optimization such that the resultant map is diffeomorphic.The end points of the source curve landmarks are mapped to their corresponding points on the target surface,while the interior points of the source curves can slide on the corresponding target curves,which provides more freedom for the surface registration than the point-based registration methods.Experiments on the real surfaces with point and curve landmarks demonstrate the efficiency,efficacy and robustness of the proposed method.
基金supported by National Science Foundation of China(No.82070630 from Bin Yi,82100658 from Yu-jie Li and 82170634 from Peng Li)National Key R&D Program of China(No.2018YFC0116702 from Bin Yi)+2 种基金Special support for Chongqing postdoctoral research project in 2020 from Yujie Li,Sichuan science and technology department research projects(2019YFS0221 from Peng Li)Chongqing Science and health joint medical research project(2020FYYX076,from Bin Yi)special support project for improving scientific and technological innovation ability of undergraduate(2021XBK19 from Xian-feng Wu).
文摘Background and Aims:The results of basic research implicate the vascular endothelial growth factor(VEGF)family as a potential target of hepatopulmonary syndrome(HPS).However,the negative results of anti-angiogenetic therapy in clinical studies have highlighted the need for markers for HPS.Therefore,we aimed to determine whether VEGF family members and their receptors can be potential biomarkers for HPS through clinical and experimental studies.Methods:Clinically,patients with chronic liver disease from two medical centers were enrolled and examined for HPS.Patients were divided into HPS,intrapulmonary vascular dilation[positive contrast-enhanced echocardiography(CEE)and normal oxygenation]and CEE-negative groups.Baseline information and perioperative clinical data were compared between HPS and non-HPS patients.Serum levels of VEGF family members and their receptors were measured.In parallel,HPS rats were established by common bile duct ligation.Liver,lung and serum samples were collected for the evaluation of pathophysiologic changes,as well as the expression levels of the above factors.Results:In HPS rats,all VEGF family members and their receptors underwent significant changes;however,only soluble VEGFR1(sFlt-1)and the sFlt-1/placental growth factor(PLGF)ratio were changed in almost the same manner as those in HPS patients.Furthermore,through feature selection and internal and external validation,sFlt-1 and the sFlt-1/PLGF ratio were identified as the most important variables to distinguish HPS from non-HPS patients.Conclusions:Our results from animal and human studies indicate that sFlt-1 and the sFlt-1/PLGF ratio in serum are potential markers for HPS.
基金This work was supported by the National Natural Sci-ence Foundation of China(No.31672496)the Natural Science Foundation of Chongqing(cstc2016jcyjA0425)+2 种基金the Chongqing University Innovation Team Building Program funded projects(CXTDX201601010)the Fundamental Research Funds for the Central Universi-ties(No.XDJK2015C129,No.2362015XK09,No.XDJK2013B020 and No.20120524)and the Scientific Research Foundation of the Chongqing University of Arts and Sciences(2017ZBX10).
文摘The Hedgehog(Hh)signaling pathway is one o f the major regulators of embryonic development and tissue homeostasis in multicellular organisms.However,the role of this pathway in the silkworm,especially in the silkworm midgut,remains poorly understood.Here,we report that Bombyx mori Hedgehog(BmHh)is expressed in most tissues of silkworm larvae and that its functions are well-conserved throughout evolution.We further demonstrate that the messenger RNA o f four Hh signaling components,BmHh ligand,BmPtch receptor,signal transducer BmSmo and transcription factor BmCi,are all upregulated following Escherichia coli or Bacillus thuringiensis infection,indicating the activation o f the Hh pathway.Simultaneously,midgut cell proliferation is strongly promoted.Conversely,the repression of Hh signal transduction with double-stranded RNA or cyclopamine inhibits the expression o f BmHh and BmCi and reduces cell proliferation.Overall,these findings provide new insights into the Hh signaling pathway in the silkworm,B.mori.
基金financially supported by the National Natural Science Foundation of China (No.51304129)the Natural Science Foundation of Shandong Province (No.ZR2013EEM005)
文摘Combined with Fourier transform infrared spectroscopy(FTIR) analysis of sodium vanadate solution,the relationship between conductivity and structure was investigated by measuring the electric conductivity of the solution under different alkali concentrations and molar ratios of NaOH to V2O5. Results suggest that the polymerization vanadium acid radical ions gradually transform into monomer with the solution diluting. When the solution is diluted to a certain extent,only the vanadium acid radical ion with V-OH chemical bond exists in the solution. At NaOH concentration of below 105.21 g·L^(-1),the vanadate anions mainly exist in the form of vanadium acid radical ion with V-OH chemical bond and the ion transference number is approximately from 0.58 to 0.82. In the medium NaOH concentration range of 105.21-117.03 g·L^(-1), the vanadate anions mostly exist in the form of vanadium acid radical ion with V-OH and V-O-V chemical bonds and the ion transference number is approximately 3.29. At NaOH concentration of above 117.03 g·L^(-1), vanadate anions exist in the form of vanadium acid radical ion with V-OH and V-O-V chemical bonds.