Hepatocellular carcinoma(HCC)is one of the most common human malignancies and main cause of cancer mortality worldwide[1].Conventional treatment for HCC consists of hepatic resection,liver transplantation and radiofre...Hepatocellular carcinoma(HCC)is one of the most common human malignancies and main cause of cancer mortality worldwide[1].Conventional treatment for HCC consists of hepatic resection,liver transplantation and radiofrequency ablation[2,3].Despite improvements in clinical treatment,the 5-year survival rate of advanced HCC patients remains low.The exploration of novel therapeutic targets and the identification of prognostic biomarkers for HCC are vital and essential to improve clinical outcomes.CKLF-like MARVEL transmembrane domaincontaining member 4(CMTM4),mapped to chromosome 16q22.1,is the most conserved member of the CMTM family.The CMTM family comprises 9 genes:CMTM 1-8 and CKLF.Proteins from CMTM family are involved in the immune system[4],the male reproductive system[5],angiogenesis regulation,and tumorigenesis.展开更多
Objective To investigate the relationship between trans, trans-muconic acid （ttMA） as benzene metabolite of occupational workers and benzene concentration in air. Methods A rapid and sensitive high-performance liqui...Objective To investigate the relationship between trans, trans-muconic acid （ttMA） as benzene metabolite of occupational workers and benzene concentration in air. Methods A rapid and sensitive high-performance liquid chromatography was developed to determine the level of urinary ttMA. ttMA was extrated from urinary samples in liquid-liquid phase a ODS （2） （5u） column （Ф4.6 min× 150 mm） and detected at wavelength 264 nm in a UV detector using vanillic acid as an internal standard. The mobile phase was acetaticacid/tetrahydrofuran/methanol/water （v/v, 1：2：10：87）. The method was validated with 56 urine samples collected from occupationally benzene-exposed individuals. Results A correlation coefficient （r = 0.9963 ） was found for ttMA ranging 0.10-10.00 μg/mL. The limit of detection was 0.10μ g/mL. The recovery and reproducibility were generally over 90%. There was a positive correlation between ttMA and benzene level in air. The equation was Y=0.859＋0.108C （before work, r=0.6200） or Y=1.980＋0.179C （after work, r=0.7930）. Conclusion This method can be used to determine and control the level of urinary ttMA in those who are occupationally exposed to benzene.展开更多
To the Editor:A20-year-old unmarried young male was hospitalized for 1 week due to an unexpected finding of heart enlargement at his regular physical examination.His parents and two sisters presented no abnormal findi...To the Editor:A20-year-old unmarried young male was hospitalized for 1 week due to an unexpected finding of heart enlargement at his regular physical examination.His parents and two sisters presented no abnormal findings in electrocardiogram (ECG) and echocardiography (Echo)examinations.He had an uncle with unexplained sudden death at the age of 47 years.展开更多
基金the National S&T Major Project of China(2018ZX10301201-008)Henan Province Science and Technology Project(2020-387).
文摘Hepatocellular carcinoma(HCC)is one of the most common human malignancies and main cause of cancer mortality worldwide[1].Conventional treatment for HCC consists of hepatic resection,liver transplantation and radiofrequency ablation[2,3].Despite improvements in clinical treatment,the 5-year survival rate of advanced HCC patients remains low.The exploration of novel therapeutic targets and the identification of prognostic biomarkers for HCC are vital and essential to improve clinical outcomes.CKLF-like MARVEL transmembrane domaincontaining member 4(CMTM4),mapped to chromosome 16q22.1,is the most conserved member of the CMTM family.The CMTM family comprises 9 genes:CMTM 1-8 and CKLF.Proteins from CMTM family are involved in the immune system[4],the male reproductive system[5],angiogenesis regulation,and tumorigenesis.
文摘Objective To investigate the relationship between trans, trans-muconic acid （ttMA） as benzene metabolite of occupational workers and benzene concentration in air. Methods A rapid and sensitive high-performance liquid chromatography was developed to determine the level of urinary ttMA. ttMA was extrated from urinary samples in liquid-liquid phase a ODS （2） （5u） column （Ф4.6 min× 150 mm） and detected at wavelength 264 nm in a UV detector using vanillic acid as an internal standard. The mobile phase was acetaticacid/tetrahydrofuran/methanol/water （v/v, 1：2：10：87）. The method was validated with 56 urine samples collected from occupationally benzene-exposed individuals. Results A correlation coefficient （r = 0.9963 ） was found for ttMA ranging 0.10-10.00 μg/mL. The limit of detection was 0.10μ g/mL. The recovery and reproducibility were generally over 90%. There was a positive correlation between ttMA and benzene level in air. The equation was Y=0.859＋0.108C （before work, r=0.6200） or Y=1.980＋0.179C （after work, r=0.7930）. Conclusion This method can be used to determine and control the level of urinary ttMA in those who are occupationally exposed to benzene.
文摘To the Editor:A20-year-old unmarried young male was hospitalized for 1 week due to an unexpected finding of heart enlargement at his regular physical examination.His parents and two sisters presented no abnormal findings in electrocardiogram (ECG) and echocardiography (Echo)examinations.He had an uncle with unexplained sudden death at the age of 47 years.
