Ophthalmology is a subject that highly depends on imaging examination.Artificial intelligence(AI)technology has great potential in medical imaging analysis,including image diagnosis,classification,grading,guiding trea...Ophthalmology is a subject that highly depends on imaging examination.Artificial intelligence(AI)technology has great potential in medical imaging analysis,including image diagnosis,classification,grading,guiding treatment and evaluating prognosis.The combination of the two can realize mass screening of grass-roots eye health,making it possible to seek medical treatment in the mode of“first treatment at the grass-roots level,two-way referral,emergency and slow treatment,and linkage between the upper and lower levels”.On the basis of summarizing the AI technology carried out by scholars and their teams all over the world in the field of ophthalmology,quite a lot of studies have confirmed that machine learning can assist in diagnosis,grading,providing optimal treatment plans and evaluating prognosis in corneal and conjunctival diseases,ametropia,lens diseases,glaucoma,iris diseases,etc.This paper systematically shows the application and progress of AI technology in common anterior segment ocular diseases,the current limitations,and prospects for the future.展开更多
AIM:To quantitatively evaluate the risk of anxiety and depression in patients with uveitis via performing a Metaanalysis.METHODS:Three electronic database(Pub Med,Embase,and Cochrane Library databases)were searched fo...AIM:To quantitatively evaluate the risk of anxiety and depression in patients with uveitis via performing a Metaanalysis.METHODS:Three electronic database(Pub Med,Embase,and Cochrane Library databases)were searched for studies recording data about uveitis and anxiety as well as depression simultaneously up to January 2021.The incidence rate and standard mean difference(SMD)with a 95%confidence interval(95%CI)were calculated to analyse the association using random-effects models based on heterogeneity tests.RESULTS:In total,12 observational studies containing 874 patients with uveitis were included.The results showed that there was a significant association between uveitis and anxiety(SMD=0.97,95%CI:0.39 to 1.54,P=0.0009)and depression(SMD=0.79,95%CI:0.51 to 1.07,P<0.00001).The overall morbidities of anxiety and depression in patients with uveitis were 39%and 17%,respectively.With subgroup analysis,the heterogeneity actually came from different kinds of uveitis.Specifically,the incidence rates of both anxiety and depression were relatively low in patients with anterior uveitis(33%and 15%),moderate in patients with infectious uveitis(46%and 22%),and high in patients with unspecified uveitis(59%and 35%).CONCLUSION:It is preliminarily indicated that patients with uveitis may have a high risk of anxiety and depression.Ophthalmologists and psychologists should pay more attention to the psychological state when dealing with patients with uveitis.Further high-quality studies with detailed direct data are needed to draw more precise conclusions.展开更多
AIM:To observe the effect of low oxygen concentration on the neural retina in human induced pluripotent stem cell(hiPSC)-derived retinal organoids(ROs).METHODS:The hiPSC and a three-dimensional culture method were use...AIM:To observe the effect of low oxygen concentration on the neural retina in human induced pluripotent stem cell(hiPSC)-derived retinal organoids(ROs).METHODS:The hiPSC and a three-dimensional culture method were used for the experiments.Generated embryoid bodies(EBs)were randomly and equally divided into hypoxic and normoxic groups.Photographs of the EBs were taken on days 38,45,and 52,and the corresponding volume of EBs was calculated.Simultaneously,samples were collected at these three timepoints,followed by fixation,sectioning,and immunofluorescence.RESULTS:The proportion of Ki67-positive proliferating cells increased steadily on day 38;this proliferationpromoting effect tended to increase tissue density rather than tissue volume.On days 45 and 52,the two groups had relatively similar ratios of Ki67-positive cells.Further immunofluorescence analysis showed that the ratio of SOX2-positive cells significantly increased within the neural retina on day 52(P<0.05).In contrast,the percentage of PAX6-and CHX10-positive cells significantly decreased following hypoxia treatment at all three timepoints(P<0.01),except for CHX10 at day 45(P>0.05).Moreover,the proportion of PAX6-/TUJ1+cells within the neural retinas increased considerably(P<0.01,<0.05,<0.05 respectively).CONCLUSION:Low oxygen promotes stemness and proliferation of neural retinas,suggesting that hypoxic conditions can enlarge the retinal progenitor cell pool in hiPSC-derived ROs.展开更多
基金Supported by National Natural Science Foundation of China(No.82101097,No.82070937).
文摘Ophthalmology is a subject that highly depends on imaging examination.Artificial intelligence(AI)technology has great potential in medical imaging analysis,including image diagnosis,classification,grading,guiding treatment and evaluating prognosis.The combination of the two can realize mass screening of grass-roots eye health,making it possible to seek medical treatment in the mode of“first treatment at the grass-roots level,two-way referral,emergency and slow treatment,and linkage between the upper and lower levels”.On the basis of summarizing the AI technology carried out by scholars and their teams all over the world in the field of ophthalmology,quite a lot of studies have confirmed that machine learning can assist in diagnosis,grading,providing optimal treatment plans and evaluating prognosis in corneal and conjunctival diseases,ametropia,lens diseases,glaucoma,iris diseases,etc.This paper systematically shows the application and progress of AI technology in common anterior segment ocular diseases,the current limitations,and prospects for the future.
文摘AIM:To quantitatively evaluate the risk of anxiety and depression in patients with uveitis via performing a Metaanalysis.METHODS:Three electronic database(Pub Med,Embase,and Cochrane Library databases)were searched for studies recording data about uveitis and anxiety as well as depression simultaneously up to January 2021.The incidence rate and standard mean difference(SMD)with a 95%confidence interval(95%CI)were calculated to analyse the association using random-effects models based on heterogeneity tests.RESULTS:In total,12 observational studies containing 874 patients with uveitis were included.The results showed that there was a significant association between uveitis and anxiety(SMD=0.97,95%CI:0.39 to 1.54,P=0.0009)and depression(SMD=0.79,95%CI:0.51 to 1.07,P<0.00001).The overall morbidities of anxiety and depression in patients with uveitis were 39%and 17%,respectively.With subgroup analysis,the heterogeneity actually came from different kinds of uveitis.Specifically,the incidence rates of both anxiety and depression were relatively low in patients with anterior uveitis(33%and 15%),moderate in patients with infectious uveitis(46%and 22%),and high in patients with unspecified uveitis(59%and 35%).CONCLUSION:It is preliminarily indicated that patients with uveitis may have a high risk of anxiety and depression.Ophthalmologists and psychologists should pay more attention to the psychological state when dealing with patients with uveitis.Further high-quality studies with detailed direct data are needed to draw more precise conclusions.
基金Supported by the National Nature Science Foundation of China(No.82070937,No.81870640,No.82000923).
文摘AIM:To observe the effect of low oxygen concentration on the neural retina in human induced pluripotent stem cell(hiPSC)-derived retinal organoids(ROs).METHODS:The hiPSC and a three-dimensional culture method were used for the experiments.Generated embryoid bodies(EBs)were randomly and equally divided into hypoxic and normoxic groups.Photographs of the EBs were taken on days 38,45,and 52,and the corresponding volume of EBs was calculated.Simultaneously,samples were collected at these three timepoints,followed by fixation,sectioning,and immunofluorescence.RESULTS:The proportion of Ki67-positive proliferating cells increased steadily on day 38;this proliferationpromoting effect tended to increase tissue density rather than tissue volume.On days 45 and 52,the two groups had relatively similar ratios of Ki67-positive cells.Further immunofluorescence analysis showed that the ratio of SOX2-positive cells significantly increased within the neural retina on day 52(P<0.05).In contrast,the percentage of PAX6-and CHX10-positive cells significantly decreased following hypoxia treatment at all three timepoints(P<0.01),except for CHX10 at day 45(P>0.05).Moreover,the proportion of PAX6-/TUJ1+cells within the neural retinas increased considerably(P<0.01,<0.05,<0.05 respectively).CONCLUSION:Low oxygen promotes stemness and proliferation of neural retinas,suggesting that hypoxic conditions can enlarge the retinal progenitor cell pool in hiPSC-derived ROs.
