Inhibition effect of miR-577 on hepatocellular carcinoma cell growth via targeting β-catenin

Objective:To investigate the expression and the regulation effect of cell growth of microRNA-577 in hepatocellular carcinoma(HCC).Methods:qRT-PCR was applied to detect the relative expression of miR-577 in 70 paired HCC and matched tumor adjacent tissues collecting from resection between March 2011 and March 2014.Pearson chi-square test was used to analyze the relationship between the miR-577 expression and clinical features.The miR-577 mimics were transfected into HepG2 cells:cell cycles were detected by flow cytometry,cell proliferation was measured by MTT assay and BrdU incorporation assay,and cell apoptosis was determined by flow cytometry and Caspase 3/7 activity analysis.The expressions ofβ-catenin were measured by immunohistochemistry.Spearman correlation analysis was used to analyze the relationship between miR-577 and p-catenin.qRT-PCR and westernblot were used to detect the expression of p-catenin in transfected HepG2 cells.Results:The relative expressions of miR-577 was significantly lower in HCC tissues compared to the matched normal tumor-adjacent tissues(P<0.05).Low expression of miR-577 was significantly associated with large tumor size(≥5 cm,P<0.05) and advanced tumor node metastasis stage(Ⅲ+Ⅳ,P<0.05).Transfection of miR-577 mimics could inhibit repress cell proliferation,enhance cell apoptosis and block the cell cycles in G_1/G_1 phase(P<0.05).miR-577 in HCC group had a significant negative correlation relationship with the expression of downstream target of β-catenin(P<0.05).Both the mRNA and protein expression in HepG2 cells were down-regulated after transfection(P<0.05).Conclusions:Low expression of miR-577 is related to the malignant clinicopathological features in HCC tissues,and miR-577 may suppress HCC growth through down-regulating p-catenin.

Mechanisms of resveratrol in the prevention and treatment of gastrointestinal cancer

Gastrointestinal(GI)cancer is one of the leading causes of cancer-related deaths worldwide.According to the Global Cancer Statistics,colorectal cancer is the second leading cause of cancer-related mortality,closely followed by gastric cancer(GC).Environmental,dietary,and lifestyle factors including cigarette smoking,alcohol intake,and genetics are the most important risk factors for GI cancer.Furthermore,infections caused by Helicobacter pylori are a major cause of GC initiation.Despite improvements in conventional therapies,including surgery,chemotherapy,and radiotherapy,the length or quality of life of patients with advanced GI cancer is still poor because of delayed diagnosis,recurrence and side effect.Resveratrol(3,4,5-trihydroxy-trans-stilbene;Res),a natural polyphenolic compound,reportedly has various pharmacologic functions including anti-oxidant,anti-inflammatory,anti-cancer,and cardioprotective functions.Many studies have demonstrated that Res also exerts a chemopreventive effect on GI cancer.Research investigating the anti-cancer mechanism of Res for the prevention and treatment of GI cancer has implicated multiple pathways including oxidative stress,cell proliferation,and apoptosis.Therefore,this paper provides a review of the function and molecular mechanisms of Res in the prevention and treatment of GI cancer.

Numerical simulation and experimental verification of a novel double-layered split die for high-pressure apparatus used for synthesizing superhard materials

Based on the principles of massive support and lateral support, a novel double-layered split die(DLSD) for high-pressure apparatus was designed to achieve a higher pressure-bearing capacity and larger sample cavity. The stress distributions of the DLSDs with different numbers of divided blocks were investigated by the finite element method and compared with the stress distributions of the conventional belt-type die(BTD). The results show that the cylinders and first-layer supporting rings of the DLSDs have dramatically smaller stresses than those of the BTD. In addition, increasing the number of divided blocks from 4 to 10 gradually increases the stress of the cylinder but has minimal influence on the stress of the supporting rings. The pressure-bearing capacities of the DLSDs with different numbers of divided blocks, especially with fewer blocks, are all remarkably higher than the pressure-bearing capacity of the BTD. The contrast experiments were also carried out to verify the simulated results. It is concluded that the pressure-bearing capacities of the DLSDs with 4 and 8 divided blocks are 1.58 and 1.45 times greater than that of the BTD. This work is rewarding for the commercial synthesis of high-quality, large-sized superhard materials using a double-layered split high-pressure die.

Effect of atorvastatin combined with trimetazidine on oxidative stress, hemorheology, and NT-proBNP, hs-CRP in patients with coronary heart disease

Objective:To explore the effect of Atorvastatin Combined with trimetazidine on oxidative stress, hemorheology and NT-proBNP and hs-CRP in patients with coronary heart disease. Method:A total of 84 patients with coronary heart disease were admitted in our hospital from February 2015 to February 2017 were randomly divided into the observation group and the control group, each group with 42 cases. The two groups received routine treatment of coronary heart disease, while the control group was treated with atorvastatin and the observation group was treated with Atorvastatin Combined with trimetazidine. Both groups were treated continuously for one month. The levels of oxidative stress indexes (SOD), malondialdehyde, (MDA), blood rheology indexes (ESR, whole blood hyposhear viscosity, whole blood hypershear viscosity, plasma viscosity, Fibrinogen (Fib) and serum N-terminal pro-brain natriuretic peptide (NT-proBNP), hypersensitive C reaction protein (hs-CRP) index in two groups were compared analytically.Results: Before treatment, there was no significant difference between the observation group and the control group in terms of oxidative stress, blood rheology and NT-proBNP and hs-CRP index. Compared with before treatment, the level of SOD in observation group and the control group was significantly increased and MDA significantly decreased. While the level of SOD in observation group was significantly higher than the control group, and MDA level was significantly lower than the control group after treatment. Compared with before treatment, the levels of hemorheology indexes included ESR, whole blood viscosity, plasma viscosity and Fib in observation group and control group were significantly decreased. After treatment, the levels of ESR, whole blood viscosity, plasma viscosity and Fib in the observation group were significantly lower than the control group. Compared with before treatment, the levels of NT-proBNP and hs-CRP in the observation group and control group were significantly decreased. After treatment, the levels of NT-proBNP and hs-CRP in the observation group were significantly lower than the control group,there was significantly statistical difference.Conclusion:Atorvastatin combined with trimetazidine can significantly reduce oxidative stress, restore normal blood rheology, and improve levels of NT-proBNP and hs-CRP in patients with coronary heart disease. This treatment is worthy of clinical promotion.

聚合物超分子体系：设计、组装与功能

高分子科学和超分子化学的相互作用产生了多种多样的聚合物超分子体系,其可能具有丰富的超分子结构,并由此带来特殊的性质与功能.我国许多高校和科研院所自20世纪80年代开始从事聚合物超分子体系的研究,并在高分子自组装、超分子聚合以及功能超分子体系等研究方面取得了一系列重要创新成果.由于篇幅有限,此综述只选择介绍了几个代表性的研究实例,展示了如何从分子设计出发,发展和建立自组装的方法,将结构构筑与功能组装结合,构建多种多样的聚合物超分子体系的思想和成果.

Home-based Early Intervention on Auditory and Speech Development in Mandarin-speaking Deaf Infants and Toddlers with Chronological Aged 7-24 Months

Background：Early auditory and speech development in home-based early intervention of infants and toddlers with hearing loss younger than 2 years are still spare in China.This study aimed to observe the development of auditory and speech in deaf infants and toddlers who were fitted with hearing aids and/or received cochlear implantation between the chronological ages of 7-24 months,and analyze the effect of chronological age and recovery time on auditory and speech development in the course of home-based early intervention.Methods：This longitudinal study included 55 hearing impaired children with severe and profound binaural deafness,who were divided into Group A （7-12 months）,Group B （13-18 months） and Group C （19-24 months） based on the chronological age.Categories auditory performance （CAP） and speech intelligibility rating scale （SIR） were used to evaluate auditory and speech development at baseline and 3,6,9,12,18,and 24 months of habilitation.Descriptive statistics were used to describe demographic features and were analyzed by repeated measures analysis of variance.Results：With 24 months of hearing intervention,78% of the patients were able to understand common phrases and conversation without lip-reading,96% of the patients were intelligible to a listener.In three groups,children showed the rapid growth of trend features in each period of habilitation.CAP and SIR scores have developed rapidly within 24 months after fitted auxiliary device in Group A,which performed much better auditory and speech abilities than Group B （P 〈 0.05） and Group C （P 〈 0.05）.Group B achieved better results than Group C,whereas no significant differences were observed between Group B and Group C （P 〉 0.05）.Conclusions：The data suggested the early hearing intervention and home-based habilitation benefit auditory and speech development.Chronological age and recovery time may be major factors for aural verbal outcomes in hearing impaired children.The development of auditory and speech in hearing impaired children may be relatively crucial in the first year＆#39;s habilitation after fitted with the auxiliary device.

SCHEDULING JOBS WITH GENERAL LEARNING FUNCTIONS

This paper deals with single-machine scheduling problems with a more general learning effect based on sum-of-processing-time. In this study, sum-of-processing-time-based learning effect means that the processing time of a job is defined by a decreasing function of the total normal processing time of jobs that come before it in the sequence. Results show that even with the introduction of the sum-of-processing-time-based learning effect to job processing times, single-machine makespan minimization problems remain polynomially solvable. The curves of the optimal schedule of a total completion time minimization problem are V-shaped with respect to iob normal orocessinz times.

Synthesis,fluorescence properties and selective Cr(Ⅲ) recognition of tetraaryl imidazole derivatives bearing thiazole group

Three tetraaryl imidazole derivatives 5a-5c bearing thiazole groups were synthesized in the presence of [Bmin]Br by one-pot reaction and their structures were fully characterized by the H NMR, IR, MS and elemental analysis. The results of UV-vis spectra and fluorescent spectra upon metal ions complexation show that compound 5a displays high selectivity and sensitivity for Cr3＋ ions. The complexation ratio of compound 5a and Cr3＋ is 1：1.

Antisense-mediated Depletion of Tomato Endoplasmic Reticulum Omega-3 Fatty Acid Desaturase Enhances Thermal Tolerance

An endoplasmic reticulum-localized tomato ω-3 fatty acid desaturase gene （LeFAD3） was isolated. The antisense tomato plants were obtained under the control of the cauliflower mosaic virus 35S promoter （35S-CaMV）. Northern blot analysis confirmed that the expression of LeFAD3 was inhibited in the tomato genome. Levels of 18：3 decreased and correspondingly levels of 18：2 increased in total lipids of leaves and roots. After heat stress, the fresh weight of the aerial parts of antisense transgenic plants was higher than that of the wild type （WT） plants. The membrane system ultrastructure of chloroplasts in leaf cells and all of the subcellular organelles in the root tips of transgenic plants remained more intact than those of WT. Relative electric conductivity increased less in transgenic plants than in WT. Under heat stress, the maximal photochemical efficiency of photosystem Ⅱ （Fv/Fm） and the O2 evolution rate decreased more in WT than in transgenic plants. These results suggested that the depletion of LeFAD3 increased the saturation of fatty acids and alleviated high temperature stress.

On Fluorophore Imaging by Diffusion Equation Model:Decompositions and Optimizations

Fluorescence imaging is a target-specific molecular imaging technology using absorption coefficient of fluorophore.For this imaging model governed by an inverse problem for the coupled diffusion system,which describes the interaction of the excitation field from several boundary sources and the corresponding emission field,we reformulate it as an optimization problem.For solving this non-quadratic optimizing problem,we propose a decomposition scheme,which extracts the horizontal information of the target from the boundary measurement data directly.The realizability of this hybrid imaging scheme is rigorously proved mathematically for cubic and ellipsoid targets,by constructing an indicator function for the horizontal location of the target explicitly.Then based on this horizonal location as a good initial guess for the iteration process,the cost functional is optimized efficiently using the trust domain scheme.Numerical implementations are provided to show the validity of the proposed scheme.