Solubility measurement,correlation and thermodynamic properties of 2,3,4-trichloro-1,5-dinitrobenzene in fifteen mono-solvents at temperatures from 278.15 to 323.15 K

The solubility of 2,3,4-trichloro-1,5-dinitrobenzene(TCDNB) was measured by a laser dynamic method over the temperature range from 278.15 K to 323.15 K under 0.1 MPa in fifteen mono-solvents(methanol,ethanol,isopropanol,n-butanol,toluene,dichloromethane,chloroform,tetrachloromethane,1,2-dichloroethane,acetone,ethyl acetate,acetonitrile,N-methylpyrrolidone(NMP),N,Ndimethylformamide dimethyl sulfoxide(DMF),dimethyl sulfoxide(DMSO).The solubility of TCDNB could be increased with increasing temperature in fifteen mono-solvents.TCDNB solubility is in the following order at 298.15 K:NMP>DMF>DMSO>toluene>acetone>ethyl acetate>dichloromethane>1,2-dich loroethane>chloroform>acetonitrile>tetrachloromethane>methanol>ethanol>n-butanol>isopropanol.The KAT-LSER model was used to investigate the solvent effect,which revealed that the hydrogen bond acidity of solvents has a greater effect on TCDNB solubility.The van't Hoff model,the modified Apelblat model,theλh model,and the non-random two liquid(NRTL)model were used to correlate the solubility of TCDNB.The calculated solubility data agreed well with the experimental data,and the modified Apelblat model fit best.Furthermore,the van't Hoff and Gibbs equations were also used to calculate the dissolution thermodynamic properties of TCDNB in various solvents.TCDNB dissolution could be an enthalpy-driven,non-spontaneous,and endothermic process in fifteen mono-solvents.The determination and fitting solubility of TCDNB,as well as the calculation of its thermodynamic properties,would be critical in the purification and crystallization of its preparation process research.

PNPLA3 I148M variant in nonalcoholic fatty liver disease:Demographic and ethnic characteristics and the role of the variant in nonalcoholic fatty liver fibrosis

Patatin-like phospholipase domain-containing 3(PNPLA3 or adiponutrin) displays anabolic and catabolic activities in lipid metabolism,and has been reported to be significantly associated with liver fat content.Variousstudies have established a strong link between the 148 isoleucine to methionine protein variant(I148M) of PNPLA3 and liver diseases,including nonalcoholic fatty liver disease(NAFLD).However,detailed demographic and ethnic characteristics of the I148 M variant and its role in the development of nonalcoholic fatty liver fibrosis have not been fully elucidated.The present review summarizes the current knowledge on the association between the PNPLA3 I148 M variant and NAFLD,and especially its role in the development of nonalcoholic fatty liver fibrosis.First,we analyze the impact of demographic and ethnic characteristics of the PNPLA3 I148 M variant and the presence of metabolic syndrome on the association between PNPLA3 I148 M and NAFLD.Then,we explore the role of the PNPLA3 I148 M in the development of nonalcoholic fatty liver fibrosis,and hypothesize the underlying mechanisms by speculating a pro-fibrogenic network.Finally,we briefly highlight future research that may elucidate the specific mechanisms of the PNPLA3 I148 M variant in fibrogenesis,which,in turn,provides a theoretical foundation and valuable experimental data for the clinical management of nonalcoholic fatty liver fibrosis.

人脑同伦功能亲和度图谱

人脑左右半球中空间对等的区域亦称同伦脑区,往往表现出功能相似性,即功能同伦.为理解人类心理行为的功能同伦规律和机制,本文基于脑连接组的双因子生成理论,提出一种人脑功能同伦研究方法:同伦功能亲和度.该方法通过计算同伦脑区的全脑功能连接模式的余弦距离,量化同伦脑区的功能亲和度.使用美国和中国“人脑连接组计划”的全脑功能磁共振影像数据库,首先绘制了“700毫秒-2毫米”时空精度的同伦功能亲和度图谱,评估了其个体差异的重测信度.其次,对该图谱的系统分析定位出人类颞顶联合区的3个特定区域,并发现了其半球偏侧化的特征规律,揭示了与注意、语言和社会认知的功能关联.最后,通过多模态脑图谱关联分析进一步探索了人脑同伦功能亲和度与遗传、进化、结构与功能组织分布的相关规律.综上,本文所提出的同伦功能亲和度方法可为人口神经科学研究提供高信效度人脑功能测量图谱.

转化人类脑图表的6E压舱石

It is of great scientific and translational promise to formulate a normative reference for the lifespan development of human brain to precisely quantify individual differences.By aggregating more than 120,000 brain imaging scans across the world,the Lifespan Brain Chart Consortium(LBCC)recently published brain charts for the human lifespan in Nature[1].

TM6SF2 E167K Variant, a Novel Genetic Susceptibility Variant, Contributing to Nonalcoholic Fatty Liver Disease

Nonalcoholic fatty liver disease(NAFLD)is one of the most common causes of liver dysfunction worldwide,and its prevalence is highly associated with genetic susceptibility.The transmembrane 6 superfamily member 2(TM6SF2)E167K variant represents a general genetic determinant of hepatic triglyceride content and lobular inflammation,and its presence appears to be directly involved in the pathogenesis and development of NAFLD.Although this variant appears to be a novel powerful modifier in the development of NAFLD,whether it is associated with an increased risk of NAFLD-refated liver fibrosis and hepatocellular carcinoma(HCC)remains to be determined.The aim of this review is to describe the functions of the TM6SF2 E167K variant and its association with NAFLD,with particular emphasis on the underlying mechanisms of its role in the development and progression of NAFLD.Additionally,the links between the TM6SF2 E167K variant and NAFLD-related liver fibrosis and HCC will be discussed.

Effects of different brush border membrane vesicle isolation protocols on proteomic analysis of Cry1Ac binding proteins from the midgut of Helicoverpa armigera

Brush border membrane vesicles （BBMV） isolated from insect midguts have been widely used to study CrylA binding proteins. Sample preparation is important in two- dimensional electrophoresis （2-DE）, so to determine a suitable BBMV preparation method in Helicoverpa armigera for 2-DE, we compared three published BBMV preparation methods mostly used in sodium dodecyl sulfate-polyacrylamide gel electrophoresis （SDS- PAGE）. All methods yielded similar types and numbers of binding proteins, but in different quantities. The Abdul-Rauf and Ellar protocol was the best of the three, but had limitations. Sufficient protein quantity is important for research involving limited numbers of insects, such as studies of insect resistance to Bacillus thuringiensis in the field. Consequently, we integrated the three BBMV isolation methods into a single protocol that yielded high quantities of BBMV proteins from H. armigera larval midguts, which proved suitable for 2- DE analysis.

Alcohol and tea consumption in relation to the risk ofnasopharyngeal carcinoma in Guangdong, China

To investigate whether alcohol and tea con-sumption has an etiological association with nasophar-yngeal carcinoma(NPC)in a high-incident population,a large scale case-control study was conducted.The study included 2846 individuals in Guangdong Province,China,with 1387 newly diagnosed cases of NPC and 1459 frequency-matched controls.Exposure histories of alcohol and tea consumption were obtained via personal inter-views.Information regarding socio-demographic charac-teristics(age,sex,education,dialect and household type),family history of NPC,Epstein-Barr virus(EBV)infection,dietary habits and other potential confounding factors was also studied.An analysis was performed using uncondi-tional logistic regression to calculate odds ratios(OR)and 95%confidence intervals(CI).The risk of NPC was found to be associated with habitual alcohol consumption and tea consumption.Tea consumption has been associated with a decreased occurrence of NPC(OR=0.62),while con-sumption of alcohol was associated with a complex effect.Specifically,moderate consumption of alcohol was associated with decreased risk of NPC,while overuse,especially strong distillate spirits,appeared to be a risk factor.

Neuroimaging brain growth charts:A road to mental health

Mental disorders are common health concerns and contribute to a heavy global burden on our modern society.It is challenging to identify and treat them timely.Neuroimaging evidence suggests the incidence of various psychiatric and behavioral disorders is closely related to the atypical development of brain structure and function.The identification and understanding of atypical brain development provide chances for clinicians to detect mental disorders earlier,perhaps even prior to onset,and treat them more precisely.An invaluable and necessary method in identifying and monitoring atypical brain development are growth charts of typically developing individuals in the population.The brain growth charts can offer a series of standard references on typical neurodevelopment,representing an important resource for the scientific and medical communities.In the present paper,we review the relationship between mental disorders and atypical brain development from a perspective of normative brain development by surveying the recent progress in the development of brain growth charts,including four aspects on growth chart utility:1)cohorts,2)measures,3)mechanisms,and 4)clinical translations.In doing so,we seek to clarify the challenges and opportunities in charting brain growth,and to promote the application of brain growth charts in clinical practice.