Decipher hydrocarbon generation and accumulation based on fluid inclusion and chronology:A case study from the Upper Paleozoic buried-hills in Huanghua Depression,Bohai Bay Basin

Deciphering hydrocarbon generation and accumulation stage is of significance to understand oil and gas evolution and seek exploration targets.Taking the Upper Paleozoic buried-hills in the Huanghua Depression,Bohai Bay Basin,as a case study,hydrocarbon generation environment and detailed accumulation process are revealed by fluid inclusions observations,Laser Raman spectroscopy,Fourier Infrared spectroscopy,and K-Ar isotope measurements.The results show that both oil and gas inclusion were captured in the quartz overgrowth,dissolved feldspar and calcite microfractures,showing blue to dark brown fluoresce.The grains containing oil inclusions index(GOI)of oil,oil&gas and gas being 25%,65%,and 10%and the inclusions with abundant methyl groups and short chains,both indicate high thermal maturity.One series of fluids inclusion is generally observed,evidenced by the concentrated homogenization temperature of 135-145℃ and salinity of 3%-15 w.t.%NaCl equiv,indicating one primary charging stage.The gas and gas&liquid inclusions mainly contain CH_(4),with also peaks indicating CO_(2) and N_(2.)The Carboniferous and Permian biomarkers show reducing environment with brackish water,with organic matter sources both from marine and continental.The relative content ofααα20RC_(27),ααα20RC_(28),andααα20RC_(29) exhibit source contributions both from algae and higher plants,and mainly of II2 to III kerogen.Both coal derived gas and oil associated hydrocarbons are identified from most of the buried-hills.Combining the fluid homogenization temperature and salinity,as well as the thermal evolution history,the hydrocarbon generated from the Upper Paleozoic was concentrated at the end of the Eocene(40 Ma±),while the beginning of charging is 60 Ma±.The Wumaying Buried-hill is of only coal derived gas and has potential for inner coal measure natural gas exploration.The results provide a detailed understanding of hydrocarbon accumulations in the study area,which can also be reference for improving petroleum exploration efficiency in similar basins.

CRISPR system contributes to deciphering the pharmaceutical compounds from TCM

Traditional Chinese medicines(TCMs),usually comprising hundreds of different compounds with distinctive physiochemical properties,have been practiced for thousands of years worldwide for the treatment of various human diseases.Significant pharmacological properties have been clarified for many TCM in recent decades,while great efforts are needed to identify the active biomedical materials from TCM due to challenges such as technical barriers to screening,isolation,characterization,and optimization[1].Recent improvements in analytical tools,genome mining,and bioengineering technologies such as CRISPR-Cas systems are overcoming such challenges and offering new opportunities,promoting the integrative studies of TCM to systematically dig out the potential pharmaceutical compounds of TCMs[2-4].

Key details of the duodenal-jejunal bypass in type 2 diabetes mellitus rats

AIM: To investigate which surgical techniques and perioperative regimens yielded the best survival rates for diabetic rats undergoing gastric bypass. METHODS: We performed Roux-en-Y gastric bypass with reserved gastric volume, a procedure in which gastrointestinal continuity was reestablished while excluding the entire duodenum and proximal jejunal loop. We observed the procedural success rate, long-term survival, and histopathological sequelae associated with a number of technical modifications. These included: use of anatomical markers to precisely identify Treitz's ligament; careful dissection along surgical planes; careful attention to the choice of regional transection sites; reconstruction using full-thickness anastomoses; use of a minimally invasive procedure with prohemostatic pretreatment and hemorrhage control; prevention of hypo-thermic damage; reduction in the length of the procedure; and accelerated surgical recovery using fast-track surgical modalities such as perioperative permissive underfeeding and goal-directed volume therapy. RESULTS: The series of modif ications we adopted reduced operation time from 110.02 ± 12.34 min to 78.39 ± 7.26 min (P < 0.01), and the procedural success rate increased from 43.3% (13/30) to 90% (18/20) (P < 0.01), with a long-term survival of 83.3% (15/18) (P < 0.01). CONCLUSION: Using a number of fast-track and damage control surgical techniques, we have successfully established a stable model of gastric bypass in diabetic rats.

Topset‑to‑forest rollover trajectories as reliable predictors of sediment‑volume partitioning into deep‑lake areas

Topset-to-forest rollover trajectories and their relation to sediment-and sand-budget partitioning into deep-lake areas are far from being well understood,as compared with their marine counterparts of shelf edges.Two quantitatively distinctive topset-to-forest rollover trajectories and clinothem-stacking patterns were recognized in the Oligocene Qikou Sag of the Bohai Bay Basin and are quantifed in terms of trajectory angles(T_(se)),topset thickness(T_(t)),forest thickness(T_(f)),bottomset thickness(T_(b)),and clinothem-set relief(R_(c)).Rising topset-to-forest trajectories have positive T_(se) of 0.15°-0.51°(averaging 0.35°).Ranges in T_(t),T_(f),T_(b),and R_(c) of their associated progradational and aggradational clinothem sets are,respectively,32.4-58.7 m(averaging 42.7 m),76.9-176.2 m(averaging 148.3 m),0 m,and 167.8-320.8 m(averaging 272.9 m).Falling topset-to-forest rollover trajectories,in contrast,have negative T_(se) of−0.12°to−0.02°(averaging−0.06°).Ranges in T_(t),T_(f),T_(b),and R_(c) of their associated progradational and downstepping clinothem sets are,respectively,0 m,266.0-395.7 m(averaging 333.4 m),441.1-542.5 m(averaging 464.1),and 874.9-922.6 m(averaging 892.5 m).These two topset-to-forest rollover trajectories and clinothem-stacking patterns are closely linked to two distinctive patterns of sediment-and sandvolume partitioning into deep-lake areas,which are quantifed in terms of T_(t),T_(b),and diferential sediment aggradation of topset segments and forest-to-bottomset compartments(As/Ad).Rising topset-to-forest rollover trajectories and associated progradational and aggradational clinothem sets are characterized by aggradational topsets(reported as T_(t) of 32.4-58.7 m),a lack of time-equivalent bottomsets,and As/Ad of 0.22-0.87(averaging 0.33),and are fronted by mud-dominated depositional deposits,with sporadic occurrence of thinner and regionally localized forest sands.They are,therefore,inefcient at delivering terrestrial sediments or sands into deep-lake settings.Falling topset-to-forest rollover trajectories and associated progradational and downstepping clinothem sets,in contrast,are characterized by toplap,erosional terminations but aggradational bottomsets(reported as T_(b) of 266.0-473.4 m),and As/Ad of 0,and are fronted by sand-rich depositional deposits,with widespread occurrence of thicker and regionally extensive time-equivalent deep-lake bottomset sands.They are,thus,efcient at delivering terrestrial sediments or sands into deep-lake settings.Topset-to-forest rollover trajectories and associated clinothem-stacking patterns are thus reliable predictors of sediment-and sand-volume partitioning into deep-lake areas,assisting greatly in developing a more dynamic stratigraphy.

Research hotspot and frontier progress of cancer under the background of precision medicine

The timely introduction and rapid development of precision medicine have provided strong theoretical support and technical support for tumor research.The treatment methods have been developed from single to multiple;the research technology has been transformed from macro to micro;the treatment drugs have been updated from systemic chemotherapy to targeted therapy and immunotherapy,and cancer has changed from a highly lethal disease to a"chronic disease".Based on the current international cancer research hotspots and treatment frontiers,this paper takes stock from five aspects,namely,treatment methods,detection technology,new drug research and development,information data and traditional Chinese medicine,with a view to"from the point to the surface","from the outside to the inside",and"the combination of Chinese and western",so as to explore the overall picture of cancer treatment and research.

Effective ingredients, potential targets and mechanism in cancers treatment of Bushen Jiedu Sanjie recipe

Objective: To analyze the active compounds, potential drug targets and corresponding therapy cancer of Bushen Jiedu Sanjie Recipe (BSJDSJR) based on network pharmacology and bioinformatics technology. Methods: The network databases including Cancer HSP, TCMSP, TCMID, TCM-PTD, TCM Database@Taiwan and DrugBank were applied to screen the active compounds, potential drug targets and corresponding cancers of BSJDSJR. Cytoscape3.3 software was used to construct the network between active compounds of Chinese medicine and the corresponding targets. Then, the enrichment of biological processes and KEGG pathways of BSJDSJR were analyzed using DAVID database. Results: According to Oral bioavailability (OB)≥30% and drug like index (DL)≥0.18, 129 active compounds in BSJDSJR were screened out and they targeted to 301 proteins. These targets were closely associated with the occurrence of various cancers, such as bladder cancer, pancreatic cancer, non-small cell lung cancer and colorectal cancer. Further investigation showed that, there were lots of active compounds in BSJDSJR are closely connected with the COX-2/β-catenin signaling pathway, STAT3 signaling pathway and MAPK/ERK signaling pathway. Conclusions: Based on the network pharmacology, the study disclosed the active chemical compounds, potential targets and possible action cancers of BSJDSJR.

Effects of mild moxibustion on intestinal microbiome and NLRP3 inflammasome in rats with 5-fluorouracil-induced intestinal mucositis

Objective:The present study investigated how mild moxibustion treatment affects the intestinal Microbiome and expression of NLRP3-related immune factors in a rat model of intestinal mucositis(IM)induced with 5-fluorouracil(5-Fu).Methods:Forty male Sprague-Dawley rats were randomly divided into control,chemotherapy,moxibustion and probiotics groups.The IM rat model was established by intraperitoneal injection of 5-Fu.Mild moxibustion treatment and intragastric probiotic administration were provided once daily for 15 days.Tissue morphology,serum levels of inflammatory factors and the expression levels of tight junction proteins,caspase-1,gasdermin D and NLRP3 were evaluated in colon tissue,through hematoxylin and eosin staining,electron microscopy,enzyme-linked immunosorbent assay,Western blotting,quantitative realtime reverse transcription polymerase chain reaction and immunofluorescence.Gut microbiome profiling was conducted through 16 S rRNA amplicon sequencing.Results:Moxibustion and probiotic treatments significantly increased the expression levels of tight junction proteins,reduced cell apoptosis and the expression levels of caspase-1,gasdermin D and NLRP3;they also decreased the serum levels of tumor necrosis factor-α,interleukin(IL)-6,IL-1βand IL-18,while increasing serum levels of IL-10.Moxibustion and probiotic treatments also corrected the reduction inα-diversity andβ-diversity in IM rats,greatly increased the proportion of the dominant bacterial genus Lactobacillus and reduced the abundance of the genera Roseburia and Escherichia in chemotherapytreated rats to levels observed in healthy animals.We also found that these dominant genera were firmly correlated with the regulation of pyroptosis-associated proteins and inflammatory factors.Finally,moxibustion and probiotic treatments elicited similar effects in regulating intestinal host-microbial homeostasis and the expression of NLRP3 inflammasome-related factors.Conclusion:Moxibustion exerts its therapeutic effect on IM by ameliorating mucosal damage and reducing inflammation.Moreover,moxibustion modulates the gut microbiota,likely via decreasing the expression levels of the NLRP3 inflammasome.

Seasonal occurrence of Aphis glycines and physiological responses of soybean plants to its feeding

The soybean aphid Aphis glycines Matsumura （Hemiptera： Aphididae） is an important pest of soybean in China. To monitor and manage this pest effectively it is neces-sary to understand its population dynamics and demographics, as well as the physiological responses of soybean plants to its feeding. In this study, using field surveying and suction-trap monitoring, we investigated the population dynamics of the soybean aphid in Xiuyan County, Liaoning Province in northeastern China during 2009-2012. The results indicatedthat the population dynamics of the soybean aphid followed a unimodal curve distribution, with the insect generally colonizing soybean fields from the middle of June to early Julyand the population reaching a peak between early July and early August. On the whole, soybean aphids occurred in suction-traps at least 2 weeks earlier than they were foundin field surveys. A total of 72 alates were collected by suction-trapping over the 4 years, with the earliest alate captures occurring on 28 May in 2009, 2011, 2012 and 4 June in 2010. The life table parameters clearly showed that this aphid had a short doubling time （4.73 ± 0.21 days）, and 7.36± 0.98 nymphs were produced by a soybean aphid adult during its lifetime （13.57 ± 0.30 days）. Finally, biochemical assays indicated that the amount of malondialdehyde and the activities of four defense-related enzymes in soybean leavessignificantly changed between 0 day and 7 days of aphid infestation. Polyphenol oxidase （PPO） and catalase （CAT） activities increased more dramatically after 1 day of aphid feed-ing. In addition, significantly higher levels of superoxide dismutase and CAT were found after aphid feeding for 7 days, whereas there was no significant change in the activitiesof peroxidase and PPO. Consequently, this study will be beneficial in determining the seasonal occurrence of the soybean aphid and selecting insect-resistant soybean varieties,and thus in developing a theoretical framework for appropriate management strategies.

中国工业技术创新的动力机制:环境管制还是政府补贴?

技术创新是企业生存和发展的原动力,研究中国工业技术创新的动力机制具有重要的理论意义和现实意义。本文运用2006—2015年中国35个工业行业的面板数据,采用固定效应和门槛回归方法,分析环境管制和政府补贴对工业企业技术创新的影响机制。研究结果表明:平均而言,环境管制对技术创新的影响不是简单的线性关系,而是'U'形关系,而政府补贴显著促进了工业企业的技术创新。同时,对污染型工业而言,环境管制对技术创新的影响依然是'U'形的,但对清洁型工业而言,加强环境管制将会抑制其技术创新。此外,政府补贴对污染型工业和清洁型工业的技术创新均具有促进作用,相比较而言,政府补贴对污染型工业技术创新的激励效应更强。通过门槛回归得出存在激励工业企业技术创新的最优环境管制区间,同时,政府补贴对工业技术创新的影响存在门槛效应。最后,本文认为,为了提升中国工业技术创新的动力,需要在环境管制与政府补贴政策之间寻求有效平衡。