UPLC-MS/MS法测定淋巴结结核患者血浆和组织异烟肼浓度的临床价值研究

目的探讨淋巴结结核患者血浆与淋巴结组织中异烟肼浓度的相关性,发掘其临床价值。方法收集武汉市肺科医院淋巴结结核患者基本信息以及不同时间点静脉血液和手术切除的颈淋巴结组织样本,建立超高效液相色谱-串联质谱(UPLC-MS/MS)法并定量检测患者血浆和颈淋巴结组织样本中的异烟肼浓度,分析血浆与淋巴结组织中异烟肼浓度的相关性。结果异烟肼血药浓度及淋巴结组织浓度的线性范围分别为0.25~16μg·m L^(-1)(r=0.999 8)、2~128μg·g^(-1)(r=0.998 8)。各质控样品的精密度、准确度、基质效应等均符合要求。血浆与淋巴结组织中的异烟肼浓度在患者静脉滴注给药后10 min(r_(s)=0.501 1,P=0.001)、30 min(r_(s)=0.402 8,P=0.005)和60 min(r_(s)=0.614 6,P=0.001)均具有正相关性。对淋巴结组织与血浆中的异烟肼浓度比值进行分析,结果发现在10,30,60 min时该比值分别为1.46(0.62,3.55),5.25(4.61,11.61),6.62(4.42,10.78)mL·g^(-1)。结论建立的UPLC-MS/MS法专属性好,灵敏度、准确度及精密度高。监测血浆异烟肼浓度可为临床淋巴结核患者合理使用异烟肼提供参考。

剥脱性青光眼与原发性开角型青光眼行青白联合手术疗效观察

目的:比较剥脱性青光眼(PXG)与原发性开角型青光眼(POAG)行青光眼白内障联合手术的临床疗效。方法:回顾性选取2015-01/2018-01于我院住院治疗的PXG患者38例40眼作为PXG组,另选取基线资料相匹配的POAG患者36例46眼作为POAG组。观察两组患者行小梁切除+白内障超声乳化摘除+人工晶状体植入术后视力、眼压、抗青光眼药物使用数量及并发症发生情况,并根据标准A(IOP≤21mmHg)、B(IOP≤18mmHg)评定手术疗效,手术完全成功定义为满足上述标准且不进行药物治疗,手术条件成功定义为满足上述标准但需进行药物治疗(≤3种)。结果:术后两组患者视力均较术前改善,眼压均明显降低,抗青光眼药物使用数量均减少。术后1、3mo两组手术完全成功率及条件成功率均无差异(P>0.05)。术后6mo,1、2a,根据标准A,PXG组手术条件成功率(90%、80%、74%)均略低于POAG组(91%、86%、82%,均P>0.05),手术完全成功率(50%、40%、29%)均明显低于POAG组(85%、64%、61%,均P<0.05);根据标准B,PXG组手术条件成功率(70%、70%、59%)略均低于POAG组(80%、80%、75%,均P>0.05),手术完全成功率(45%、30%、18%)均明显低于POAG组(78%、61%、54%,均P<0.05)。结论:PXG及POAG患者实施青光眼白内障联合手术后能够显著提高视力、降低眼压,两者短期手术成功率相似,但PXG患者远期手术成功率明显偏低,且无法达到低标准眼压控制。

The principle of a navigation constellation composed of SIGSO communication satellites

The Chinese Area Positioning System （CAPS）, a navigation system based on geostafionary orbit （GEO） communication satellites, was developed in 2002 by astronomers at Chinese Academy of Sciences. Extensive positioning experiments of CAPS have been performed since 2005. On the basis of CAPS, this paper studies the principle of a navigation constellation composed of slightly inclined geostationary orbit （SIGSO） communication satellites. SIGSO satellites are derived from GEO satellites which are near the end of their operational life by inclined orbit operation. Considering the abundant frequency resources of SIGSO satellites, multi-frequency observations could be conducted to enhance the precision of pseudorange measurements and ameliorate the positioning performance. A constellation composed of two GEO satellites and four SIGSO satellites with an inclination of 5° can provide service to most of the territory of China with a maximum position dilution of precision （PDOP） over 24 h of less than 42. With synthetic utilization of the truncated precise code and a physical augmentation factor in four frequencies, the navigation system with this constellation is expected to obtain comparable positioning performance to that of the coarse acquisition code of the Global Positioning System （GPS）. When the new method of code-carrier phase combinations is adopted, the system has the potential to possess commensurate accuracy with the precise code in GPS. Additionally, the copious frequency resources can also be used to develop new anti-interference techniques and integrate navigation and communication.

Atmospheric and Oceanic Excitations to LOD Change on Quasi-biennial Tune Scales

We use wavelet transform to study the time series of the Earth＇s rotation rate （length-of-day, LOD）, the axial components of atmospheric angular momentum （AAM） and oceanic angular momentum （OAM） in the period 1962-2005, and discuss the quasi-biennial oscillations （QBO） of LOD change. The results show that the QBO of LOD change varies remarkably in amplitude and phase. It was weak before 1978, then became much stronger and reached maximum values during the strong El Nino events in around 1983 and 1997. Results from analyzing the axial AAM indicate that the QBO signals in axial AAM are extremely consistent with the QBOs of LOD change. During 1963-2003, the QBO variance in the axial AAM can explain about 99.0% of that of the LOD, in other words, all QBO signals of LOD change are almost excited by the axial AAM, while the weak QBO signals of the axial OAM are quite different from those of the LOD and the axial AAM in both time-dependent characteristics and magnitudes. The combined effects of the axial AAM and OAM can explain about 99.1% of the variance of QBO in LOD change during this period.

Incurable and refractory spinal cystic echinococcosis: A case report

BACKGROUND Although the incidence and cure rate of spinal hydatidosis are low,the recurrence rate of spinal hydatidosis is high,and the prognosis of spinal hydatidosis is poor.Therefore,we report a typical case of refractory spinal hydatidosis to increase spine surgeons’awareness of the disease and reduce misdiagnosis and recurrence.CASE SUMMARY A 48-year-old man presented with back pain,significant weight loss,and paralysis of both lower limbs.The patient was misdiagnosed with spinal tuberculosis in an outside hospital.However,spinal magnetic resonance imaging(MRI)showed hyperintense cystic components on T2-weighted images and hypointensity on T1-weighted images.A lobulated,multiocular,honeycombappearance,septated cystic mass protruding intraspinally and compressing the spinal cord at segments T8–T9 was present.Paravertebral polycystic lobular lesions presented as a“bunch of grapes”.The ELISA test result for Echinococcus granulosus was positive.Then,a diagnosis of spinal hydatidosis and lung hydatid disease was made,and the patient underwent left transthoracic approach lobectomy,paravertebral lesion debridement,and subtotal vertebrectomy with vertebral body replacement of segments T8 and T9 by a mesh cage.The patient also underwent albendazole chemotherapy before and after surgery.One year after stopping the drug therapy,the patient developed recurrent T5 vertebral lesions and underwent a second subtotal vertebrectomy surgery.The patient is currently in good condition and is receiving long-term medication and follow-up.CONCLUSION The MRI feature of a“bunch of grapes”is a typical imaging indication of spinal hydatidosis.Subtotal vertebrectomy is a risk factor for postoperative recurrence.Total spondylectomy makes it possible to cure spinal hydatidosis,but antiparasitic drug therapy is also an important supplementary therapy to multimodal therapy.It is preferable for patients with spinal hydatidosis to receive life-long antiparasitic medication therapy and follow-up.

Short-Term Period Variation of Relative Sunspot Numbers

We use wavelet transform to analyze the daily relative sunspot number series over solar cycles 10-23. The characteristics of some of the periods shorter than - 600-day are discussed. The results exhibit not only the variation of some short periods in the 14 solar cycles but also the characteristics and differences around solar peaks and valley years. The short periodic components with larger amplitude such as ～27, ～ 150 and ～360-day are obvious in some solar cycles, all of them are time-variable, also their lengths and amplitudes are variable and intermittent in time. The variable characteristics of the periods are rather different in different solar cycles.

Ohba's Conjecture is True for Graphs K_(t+2,3,2*(k-t-2),1*t)

A graph G is called chromatic-choosable if its choice number is equal to its chromatic number, namely ch（G） = X（G）. Ohba＇s conjecture states that every graph G with 2X（G）＋ 1 or fewer vertices is chromatic- choosable. It is clear that Ohba＇s conjecture is true if and only if it is true for complete multipartite graphs. Recently, Kostochka, Stiebitz and Woodall showed that Ohba＇s conjecture holds for complete multipartite graphs with partite size at most five. But the complete multipartite graphs with no restriction on their partite size, for which Ohba＇s conjecture has been verified are nothing more than the graphs Kt＋3,2.（k-t-l）,l.t by Enotomo et al., and gt＋2,3,2.（k-t-2）,l.t for t ≤ 4 by Shen et al.. In this paper, using the concept of f-choosable （or Lo-size-choosable） of graphs, we show that Ohba＇s conjecture is also true for the graphs gt＋2,3,2.（k-t-2）,l.t when t ≥ 5. Thus, Ohba＇s conjecture is true for graphs Kt＋2,3,2,（k-t-2）,l＊t for all integers t 〉 1.

A Lindqvist-type [W_(6)O_(19)]^(2-) organic–inorganic compound: synthesis, characterization, antibacterial activity and preliminary studies on the mechanism of action

In this paper,a new organic–inorganic hybrid compound[Co(L)_(2)]2[W_(6)O_(19)](1)(HL=2-acetylpyridine thiosemicarbazone)was prepared and characterized.The compound 1 exhibits remarkable antibacterial activity by determination of minimum inhibitory concentration(MIC)against Staphylococcus aureus(S.aureus,0.06μg·mL^(−1))and Escherichia coli(E.coli,0.24μg·mL^(−1)),respectively.Furthermore,the potential mechanism of compound 1 was studied in detail.The potential causes of bacteria death were cell wall/membrane disruption,inhibition of intracellular respiratory chain dehydrogenases(RCD)activity,destruction of reactive oxygen species(ROS)and depletion of glutathione(GSH).