AIM:To test the pathogenicity of pseudorabies virus(PRV)variant HN1201 and compare its pathogenicity with a classical PRV Fa strain.METHODS:The pathogenicity of the newly-emerging PRV variant HN1201 was evaluated by d...AIM:To test the pathogenicity of pseudorabies virus(PRV)variant HN1201 and compare its pathogenicity with a classical PRV Fa strain.METHODS:The pathogenicity of the newly-emerging PRV variant HN1201 was evaluated by different inoculating routes,virus loads,and ages of pigs.The classical PRV Fa strain was then used to compare with HN1201 to determine pathogenicity.Clinical symptoms after virus infection were recorded daily and average daily body weight was used to measure the growth performance of pigs.At necropsy,gross pathology and histopathology were used to evaluate the severity of tissue damage caused by virus infection.RESULTS:The results showed that the efficient infection method of RPV HN1201 was via intranasal inoculation at 107 TCID50,and that the virus has high pathogenicity to 35-to 127-d old pigs.Compared with Fa strain,pigs infected with HN1201 showed more severe clinical symptoms and pathological lesions.Immunochemistry results revealed HN1201 had more abundant antigen distribution in extensive organs.CONCLUSION:All of the above results suggest that PRV variant HN1201 was more pathogenic to pigs than the classical Fa strain.展开更多
A superamphiphobic(SAP)surface was fabricated by electrodepositing Cu-Ni micro-nano particles on aluminum substrate and modifying via 1 H,1 H,2 H,2 Hperfluorodecyltrimethoxysilane.Scanning electron microscopy,X-ray di...A superamphiphobic(SAP)surface was fabricated by electrodepositing Cu-Ni micro-nano particles on aluminum substrate and modifying via 1 H,1 H,2 H,2 Hperfluorodecyltrimethoxysilane.Scanning electron microscopy,X-ray diffraction,energydispersive X-ray spectroscopy,and Fourier-transform infrared spectroscopy were employed to investigate the morphology and chemical composition.The results showed that the SAP surface had three-dimensional micro-nano structures and exhibited a maximum water contact angle of 160.0°,oil contact angle of 151.6°,a minimum water slide angle of 0°and oil slide angle of 9°.The mechanical strength and chemical stability of the SAP surface were tested further.The experimental results showed that the SAP surface presented excellent resistance to wear,prominent acid-resistance and alkali-resistance,self-cleaning and anti-fouling properties.展开更多
基金Supported by Major Science and Technology Program in Henan Province,No.131100110200
文摘AIM:To test the pathogenicity of pseudorabies virus(PRV)variant HN1201 and compare its pathogenicity with a classical PRV Fa strain.METHODS:The pathogenicity of the newly-emerging PRV variant HN1201 was evaluated by different inoculating routes,virus loads,and ages of pigs.The classical PRV Fa strain was then used to compare with HN1201 to determine pathogenicity.Clinical symptoms after virus infection were recorded daily and average daily body weight was used to measure the growth performance of pigs.At necropsy,gross pathology and histopathology were used to evaluate the severity of tissue damage caused by virus infection.RESULTS:The results showed that the efficient infection method of RPV HN1201 was via intranasal inoculation at 107 TCID50,and that the virus has high pathogenicity to 35-to 127-d old pigs.Compared with Fa strain,pigs infected with HN1201 showed more severe clinical symptoms and pathological lesions.Immunochemistry results revealed HN1201 had more abundant antigen distribution in extensive organs.CONCLUSION:All of the above results suggest that PRV variant HN1201 was more pathogenic to pigs than the classical Fa strain.
基金supported by Science and Technology Department of Sichuan Province(2017JZ0021,2017SZ0039)Education Department of Sichuan Province(17ZA0298)Innovative Training Program for College Students of Sichuan Province(No.201810626118)。
文摘A superamphiphobic(SAP)surface was fabricated by electrodepositing Cu-Ni micro-nano particles on aluminum substrate and modifying via 1 H,1 H,2 H,2 Hperfluorodecyltrimethoxysilane.Scanning electron microscopy,X-ray diffraction,energydispersive X-ray spectroscopy,and Fourier-transform infrared spectroscopy were employed to investigate the morphology and chemical composition.The results showed that the SAP surface had three-dimensional micro-nano structures and exhibited a maximum water contact angle of 160.0°,oil contact angle of 151.6°,a minimum water slide angle of 0°and oil slide angle of 9°.The mechanical strength and chemical stability of the SAP surface were tested further.The experimental results showed that the SAP surface presented excellent resistance to wear,prominent acid-resistance and alkali-resistance,self-cleaning and anti-fouling properties.
