短时间吸入不同浓度七氟醚对幼鼠空间学习记忆能力及海马神经细胞凋亡的影响

目的观察短时间吸入不同浓度七氟醚对幼鼠空间学习记忆能力及海马神经细胞凋亡的影响。方法选择80只SD幼鼠,均为1月龄,采取随机数字表法将幼鼠分为对照组(单纯吸氧2 h)、1%七氟醚组(吸入1%七氟醚2 h)、2%七氟醚组(吸入2%七氟醚2 h)与3%七氟醚组(吸入3%七氟醚2 h),每组20只。吸入结束12 h后,各组取5只开展Morris水迷宫实验,5只取海马组织行苏木精-伊红染色,5只通过流式细胞分析仪进行细胞凋亡测定,5只通过激光共聚焦显微镜进行神经元细胞游离钙离子浓度测定。结果2%七氟醚组、3%七氟醚组平台象限停留时间较对照组缩短(P<0.05),穿台次数较对照组减少(P<0.05)。2%七氟醚组、3%七氟醚组凋亡神经元数目较对照组增加(P<0.05)。2%七氟醚组、3%七氟醚组早期凋亡率、晚期凋亡率较对照组增加(P<0.05)。2%七氟醚组、3%七氟醚组荧光强度较对照组增加(P<0.05),钙离子浓度较对照组升高(P<0.05)。结论短时间内吸入1%七氟醚并不会对幼鼠空间学习记忆能力造成影响,短时间吸入2%或者3%七氟醚可降低其空间学习记忆能力,促进海马神经细胞凋亡,提高细胞钙离子浓度可能为触发凋亡的机制。

Investigation and analysis of attitudes and knowledge of aging among students in different majors

Objective:To investigate the attitude and knowledge score of the elderly in different majors.Methods:A total of 265 students were surveyed by self-designed questionnaire that consists of three parts:general information,Kogan’s attitude toward older people(KAOP)scale,and knowledge questionnaire.Results:There were significant differences between the attitudes of the elderly and the aging knowledge scores for students who have taken care of the elderly and who have been cared for by their grandparents(P=0.04;P=0.02).Students who had experience in caring for the elderly scored higher.Students who were taken care of by their grandparents during the childhood were more active in the elderly.The scores of attitudes appreciation and attitude scores of elderly were statistically significant between nursing students and non-nursing students(P=0.01;P=0.03),and the non-nursing students’attitude appreciation and attitude scores were higher than those of nursing students.Conclusions:The nursing students have a more positive attitude to the elderly,but the level of aging knowledge is lower and lower than that of non-nursing students,which should be further improved.

Morphology and leaf nutrition of introduced Robinia pseudoacacia clones

In order to study and popularize clones from the introduced Robinia pseudoacacia, morphological charac- teristics and leaf nutrition of samples of a three-year-old stand were observed and analyzed during its growing season. Combined with data on rooting ability, height and basal diameter, comparisons were made on morphological characteris- tics, growth traits and leaf nutrition of 11 introduced R. pseudoacacia clones （two from Hungary and nine from South Ko- rea） and two domestic clones, The results show that there are significant differences in growth and morphological char- acteristics among the 13 clones, but no significant differences in the contents of crude protein and crude fibers in the leaves （p 〉 0.05）. Height and basal diameter growth of clones B and G were recorded as the fastest, while clone H1 was the slowest. Clone H2 had the largest leaflets, three times as large as other clones, while K4 had the heaviest dry weight per 100 leaflets because of its thick leaves. The 13 clones can be divided into four classes based on the number of leaf- lets per compound leaf, i.e., 1-3, 13-17, 15-23 and 21-25. There were significant differences in thorn size： H2, with the largest leaflets had the smallest thorns. While of course all clones produced roots, there were significant differences; clones 2N, K5 and B had many adventitious roots, while clones K3, K4 and H1 had few. Correlation between content of crude protein and （length x width of leaf） was positive （p 〈 0.05）, while correlations of the content of crude protein, with the number of leaflets per compound leaf, petiole length of compound leaf and thorn length were negative （p 〈 0.01）. Plant height and basal diameter were positively correlated with each other （p 〈 0.01） and negatively correlated with base width of thorns （p 〈 0.05）.

Study on the psychological reaction and coping strategies of nursing students under the spread of COVID-19

Background:To understand the psychological reaction and coping strategies of infectious diseases among nursing students.Methods:A convenience sampling method was used to select 455 nursing students in the school,and a questionnaire survey was conducted on the general data table and the psychological reaction and coping strategy scale of infectious diseases of college students and coronavirus disease 2019(COVID-19)was used as an example of the infectious diseases.Results:the psychological reaction of nursing students was related to gender(P=0.006),age(P=0.001),education level(P=0.019),professional category of senior high school(P=0.014),whether they were student cadres(P<0.001)and whether there were immediate relatives engaged in medical and health related work(P=0.022).Different education level(P=0.017),different age(P=0.041),different residence(P=0.003),high school professional category(P=0.008)and whether there are immediate relatives engaged in medical and health related work(P=0.021)are related to their coping strategies.Conclusion:Nursing students with different demographic data have different psychological reactions and coping strategies.In the future research on the infectious diseases related to nursing students,it is suggested to focus on the female,the younger,the less educated,the liberal arts students and the nursing students who have no immediate family members engaged in medical and health related work,so as to promote the physical and mental health development of the nursing students in school.

Advances in the application of Ba Duanjin in the treatment of mild cognitive impairment in the elderly

Mild cognitive impairment,as a preventable component in the development of irreversible Alzheimer's disease,plays an important role in the prevention and control of mild cognitive impairment.As a traditional aerobic exercise,Ba Duanjin has a certain influence on mild cognitive impairment from the perspective of traditional Chinese medicine,specific diseases and neurology.This article summarized the evidence of Ba Duanjin in the application of mild cognitive impairment,in order to provide reference for the clinical application of Ba Duanjin in the future.

The application of the mobile information technology in continuous care for hypertension patients

Objective： This article introduces the concepts of mobile information technology and continuous care, develops a systematic review of the application of mobile information technology for continuous care in patients with hypertension from three aspects： mobile communication technology, internet technology, health management platform, and puts forward the existing questions, influence and the research prospect of the application of mobile information tech- nology using in hypertension continuous care, in order to provide evidence for the application of mobile information technology in continuous care for hypertension patients in clinical practice.

A Class of Stable and Conservative Finite Difference Schemes for the Cahn-Hilliard Equation

In this paper, we propose a class of stable finite difference schemes for the initial-boundary value problem of the Cahn-Hilliard equation. These schemes are proved to inherit the total mass conservation and energy dissipation in the discrete level. The dissipation of the total energy implies boundness of the numerical solutions in the discrete H1 norm. This in turn implies boundedness of the numerical solutions in the maximum norm and hence the stability of the difference schemes. Unique existence of the numerical solutions is proved by the fixed-point theorem. Convergence rate of the class of finite difference schemes is proved to be O（h2 ＋ r2） with time step T and mesh size h. An efficient iterative algorithm for solving these nonlinear schemes is proposed and discussed in detail.