Plants are known to be efficient hosts for the production of mammalian therapeutic proteins.However,plants produce complex N-glycans bearingβ1,2-xylose and coreα1,3-fucose residues,which are absent in mammals.The im...Plants are known to be efficient hosts for the production of mammalian therapeutic proteins.However,plants produce complex N-glycans bearingβ1,2-xylose and coreα1,3-fucose residues,which are absent in mammals.The immunogenicity and allergenicity of plant-specific Nglycans is a key concern in mammalian therapy.In this study,we amplified the sequences of 2 plant-specific glycosyltransferases from Nicotiana tabacum L.cv Bright Yellow 2(BY2),which is a well-established cell line widely used for the expression of therapeutic proteins.The expression of the endogenous xylosyltranferase(XylT)and fucosyltransferase(FucT)was downregulated by using RNA interference(RNAi)strategy.The xylosylated and core fucosylated N-glycans were significantly,but not completely,reduced in the glycoengineered lines.However,these RNAi-treated cell lines were stable and viable and did not exhibit any obvious phenotype.Therefore,this study may provide an effective and promising strategy to produce recombinant glycoproteins in BY2 cells with humanized N-glycoforms to avoid potential immunogenicity.展开更多
基金This work was supported by grants from National Natural Science Foundation of China(Grant No.31030047)the European Commission Sixth Framework Program(Grant No.SP22-CT-2004-511060).
文摘Plants are known to be efficient hosts for the production of mammalian therapeutic proteins.However,plants produce complex N-glycans bearingβ1,2-xylose and coreα1,3-fucose residues,which are absent in mammals.The immunogenicity and allergenicity of plant-specific Nglycans is a key concern in mammalian therapy.In this study,we amplified the sequences of 2 plant-specific glycosyltransferases from Nicotiana tabacum L.cv Bright Yellow 2(BY2),which is a well-established cell line widely used for the expression of therapeutic proteins.The expression of the endogenous xylosyltranferase(XylT)and fucosyltransferase(FucT)was downregulated by using RNA interference(RNAi)strategy.The xylosylated and core fucosylated N-glycans were significantly,but not completely,reduced in the glycoengineered lines.However,these RNAi-treated cell lines were stable and viable and did not exhibit any obvious phenotype.Therefore,this study may provide an effective and promising strategy to produce recombinant glycoproteins in BY2 cells with humanized N-glycoforms to avoid potential immunogenicity.
