Relationship between the high-risk HPV infection and the expression of oncogenes, anti-oncogenes in cervical dysplasia

Objective:To study the relationship between the infection of high-risk HPV in cervical precancerous lesion and the expression of oncogene, anti-oncogene.Methods:218 cases ofcervical intraepithelial neoplasia patients in our hospital during May 2014–May 2016 were chosed and divided into high-risk HPV group (n=107), low-risk HPV group (n=111) according to cervical tissue HPV test;another 100 cases of patients received cervical biopsy and confirmed as benign lesions were enrolled in the control group. RT-PCR method was used to detect the mRNA expression of proto-oncogene and anti-oncogene in three groups, Western-blot method was used to detect the protein expression of Sox-2 and Wnt/β-catenin signal pathway.Results: mRNA expression of oncogene DEK, Bmi-1, c-fos, K-ras, Prdx4 in high-risk HPV group were higher than low-risk HPV group and control group (P<0.05);mRNA expression of anti-oncogene P27, P16, DAPK, PTEN, eIF4E3 in high-risk HPV group were lower than low-risk HPV group and control group (P<0.05);expression of Sox-2 and Wnt/β-catenin signaling pathway protein Sox-2,β-catenin, wnt-1, wnt-3a in high-risk HPV group were higher than low-risk HPV group and control group (P<0.05).Conclusions:High-risk HPV infection can increase the expression of oncogenes and reduce the expression of anti-oncogenes in cervical dysplasia tissues on Sox-2- and Wnt/β-catenin signaling pathway manners.

Successful treatment of lichen amyloidosis coexisting with atopic dermatitis by dupilumab:Four case reports

BACKGROUND Lichen amyloidosis(LA)is a chronic,severely pruritic skin disease,which is the most common form of primary cutaneous amyloidosis.The treatment of LA has been considered to be difficult.LA may be associated with atopic dermatitis(AD),and in this setting,the treatment options may be more limited.Herein,we report four cases of LA associated with AD successfully treated by dupilumab.CASE SUMMARY In this article,we describe four cases of patients who presented with recurrent skin rash accompanied by severe generalized intractable pruritus,diagnosed with refractory LA coexisting with chronic AD.Previous treatments had not produced any apparent improvement.Thus,we administered dupilumab injection subcutaneously at a dose of 600 mg for the first time and 300 mg every 2 wk thereafter.Their lesions all markedly improved.CONCLUSION Dupilumab may be a new useful treatment for LA coexisting with AD.

四瓣技术在改良结膜囊泪囊鼻腔吻合术中的应用

目的:探讨四瓣技术在改良结膜囊泪囊鼻腔吻合术中应用的安全性及有效性。方法:回顾性临床研究。选取2017-09/2020-06在我院就诊的泪道疾病患者28例28眼作为研究对象,均行结膜囊泪囊鼻腔吻合术。术后随访6~12mo,观察患者手术效果、满意度及术后并发症情况。结果:本组患者术后荧光素钠染料排泄试验阳性率为96%(27/28),手术成功率为96%(27/28),其中治愈率为68%(19/28),好转率为28%(8/28),无效率为4%(1/28),患者总体满意度为93%(26/28)。术后并发症包括眼部异物感(28眼)、球结膜充血(11眼)、鼻腔结膜囊渗血(8眼)及泪道引流管脱出(1眼)。结论:四瓣技术用于改良结膜囊泪囊鼻腔吻合术手术成功率高,并发症少,安全有效。

Modeling of the whole process of shock wave overpressure of freefield air explosion

The waveform of the explosion shock wave under free-field air explosion is an extremely complex problem.It is generally considered that the waveform consists of overpressure peak,positive pressure zone and negative pressure zone.Most of current practice usually considers only the positive pressure.Many empirical relations are available to predict overpressure peak,the positive pressure action time and pressure decay law.However,there are few models that can predict the whole waveform.The whole process of explosion shock wave overpressure,which was expressed as the product of the three factor functions of peak,attenuation and oscillation,was proposed in the present work.According to the principle of explosion similarity,the scaled parameters were introduced and the empirical formula was absorbed to form a mathematical model of shock wave overpressure.Parametric numerical simulations of free-field air explosions were conducted.By experimental verification of the AUTODYN numerical method and comparing the analytical and simulated curves,the model is proved to be accurate to calculate the shock wave overpressure under free-field air explosion.In addition,through the model the shock wave overpressure at different time and distance can be displayed in three dimensions.The model makes the time needed for theoretical calculation much less than that for numerical simulation.

Anti-Hepatitis B Virus Drugs in Clinical and Preclinical Development

Up to date, there are two types of drugs approved to treat hepatitis B： interferons and nucleos （t） ide analogues. However, the therapies are limited in the clinical context because of the negative side effects of interferon-or and the development of substantial viral resistance to nucleos （t） idic inhibitors. Therefore, new drugs with novel structures and mechanisms are needed. In this article, the drugs approved by FDA or the European Commission for treating chronic hepatitis B virus infection, as well as those under clinical trials, and several compounds in preclinical studies are reviewed. Additionally, some potential targets and strategies to combat chronic hepatitis B virus infection are discussed.

Research on Micro-Flow Self-Sensing Actuators Based on Piezoelectric Ceramic Stack `

The paper is concerned with the micro-flow self-sensing actuators,the work of which is based on the secondary piezoelectric effect. The piezoelectric ceramic stack can yield micro-displacement due to its first inverse piezoelectric effect. Therefore,we apply this micro-displacement to cell micro-flow injection. Moreover, due to the charge of the secondary direct piezoelectric effect,the piezoelectric ceramic stack is able to detect the force and displacement in the injection by itself. The experiments of first inverse piezoelectric effect and secondary direct piezoelectric effect are conducted. The experiment results show that,subjected to 0- 60 V input,the piezoelectric ceramic stack can generate 13. 45 μm displacement,and control accuracy can achieve 2 nm. It can completely meet the needs of cell micro-flow injection. Also,the experiments demonstrate that the micro-displacement due to the first inverse piezoelectric effect can be well self-sensed by the electric charge due to the secondary direct piezoelectric effect.

Mortality and Morbidity of Extremely Low Birth Weight Infants in the Mainland of China： A Multi-center Study

Background： With the progress ofperinatal medicine and neonatal technology, more and more extremely low birth weight （ELBW） survived all over the world. This study was designed to investigate the short-term ontcomes of ELBW infants during their Neonatal Intensive Care Unit （NICU） stay in the mainland of China. Methods： All infants admitted to 26 NICUs with a birth weight （BW） 〈1000 g were included between January 1,2011 and December 31,2011. All the data were collected retrospectively from clinical records by a prospectively designed questionnaire. The data collected from each NICU transmitted to the main institution where the results were aggregated and analyzed. Categorical variables were performed with Pearson Chi-square test. Binary Logistic regression analysis was used to detect risk factors. Results： A total of 258 ELBW infants were admitted to 26 NICUs, of whom the mean gestational age （GA） was 28.1 ± 2.2 weeks, and the mean BW was 868 ± 97 g. The overall survival rate at discharge was 50.0%. Despite aggressive treatment 60 infants （23.3%） died and another 69 infants （26.7%） died after medical care withdrawal. Furthermore, the survival rate was significantly higher in coastal areas than inland areas （53.6% vs. 35.3%, P = 0.019）. BW 〈750 g and GA 〈28 weeks were the largest risk factors, and being small for gestational age was a protective factor related to mortality. Respiratory distress syndrome was the most common complication. The incidence of patent ductus arteriosus, intraventricular hemorrhage, periventricular leukomalacia, bronchopulmonary dysplasia, retinopathy of prematurity was 26.2%, 33.7%, 6.7%, 48.1%, and 41.4%, respectively. Ventilator associated pneumonia was the most common hospital acquired infection during hospitalization. Conclusions： Our study was the first survey that revealed the present status of ELBW infants in the mainland of China. The mortality and morbidity of ELBW infants remained high as compared to other developed countries.

Analysis of In-hospital Neonatal Death in the Tertiary Neonatal Intensive Care Unit in China： A Multicenter Retrospective Study

Background：Globally,the proportion of child deaths that occur in the neonatal period remains a high level of 37-41%.Differences of cause in neonate death exist in different regions as well as in different economic development countries.The specific aim of this study was to investigate the causes,characteristics,and differences of death in neonates during hospitalization in the tertiary Neonatal Intensive Care Unit （NICU） of China.Methods：All the dead neonates admitted to 26 NICUs were included between January 1,2011,and December 31,2011.All the data were collected retrospectively from clinical records by a designed questionnaire.Data collected from each NICU were delivered to the leading institution where the results were analyzed.Results：A total of 744 newborns died during the l-year survey,accounting for 1.2% of all the neonates admitted to 26 NICUs and 37.6% of all the deaths in children under 5 years of age in these hospitals.Preterm neonate death accounted for 59.3% of all the death.The leading causes of death in preterm and term infants were pulmonary disease and infection,respectively.In early neonate period,pulmonary diseases （56.5%） occupied the largest proportion ofpreterm deaths while infection （27%） and neurologic diseases （22%） were the two main causes of term deaths.In late neonate period,infection was the leading cause of both preterm and term neonate deaths.About two-thirds of neonate death occurred after medical care withdrawal.Of the cases who might survive if receiving continuing treatment,parents＇ concern about the long-term outcomes was the main reason of medical care withdrawal.Conclusions：Neonate death still accounts for a high proportion of all the deaths in children under 5 years of age.Our study showed the majority of neonate death occurred in preterm infants.Cause of death varied with the age of death and gestational age.Accurate and prompt evaluation of the long-term outcomes should be carried out to guide the critical decision.

Management strategies of neonatal jaundice during the coronavirus disease 2019 outbreak

The outbreak of coronavirus disease 2019 (COVID-19;formally known as 2019-nCoV) has become a most challenging health emergency.Owing to rigorous quarantine and control measures taken in China,routine neonatal health surveillance and follow-up have become challenging.Without follow-up surveillance,some rapid and progressive newborn diseases,such as bilirubin encephalopathy,may be ignored.The characteristics of onset age of kernicterus suggest that monitoring of bilirubin level at home provides a useful way to alert hospital visits and to prevent the development of extremely hyperbilirubinemia.Therefore,we developed an online follow-up program for convenient monitoring of bilirubin level of newborns that is based on our practical experiences.The aim is to make our management strategies of neonatal jaundice tailored to the infection prevention and control during the COVID-19 epidemic.

Continuing interventions in a quality improvement bundle to reduce bronchopulmonary dysplasia

Bronchopulmonary dysplasia(BPD)is a chronic lung disease of premature infants that results from an imbalance between lung injury and repair in the developing lung[1],BPD is the most common respiratory morbidity with multiple factors implicated in its etiopathogenesis for preterm infants.Despite the scientific advances in the field of neonatology,the incidence of BPD has largely been unchanged due to increased survival of extremely premature infants.Definition of BPD continues to be challenging and is used differently in the literature.BPD was first defined by Northway et al.