Preparation technology of Naoxueling capsules

Objective To optimize the preparation of the Naoxueling capsules.Methods To optimize the extraction of the Naoxueling prescription successively with alcohol and water,several principal ingredients in the final extracts were evaluated and data were analyzed by the orthogonal test.The capsule-molding process was investigated by measuring the angle of repose and the moisture absorption percentage.Results The optimum process of the alcohol extraction was the refluxing of herbs first in twelve-fold volume of 75% ethanol for 2 h,then in eight-fold volume of 75% ethanol for 1.5 h,and finally in seven-fold volume of 75% ethanol for 0.5 h.Meanwhile,the best water extraction was performed by boiling samples in twelve-fold amount of water for 2 h followed by another 1 h in eight-fold amount of water.To mold the capsule,the appropriate ratio of micro-powder to dextrin was 20:1.Conclusion The preparation technology of the Naoxueling capsules is reasonable and feasible,which provides evidences for the industrial manufacture.

10-23DRzs mediated by fluorescent silica nanoparticles inhibit expression of HBV

Objective To prepare fluorescent silica nanoparticles(FSNPs) carrying 10-23 deoxyribozymes(10-23DRzs) and to evaluate their inhibitory effect on human hepatitis B virus(HBV).Methods The FSNPs were prepared by microemulsion method and further modified by NaCl.Their diameter and the Zeta potential were tested.The HBV-specific 10-23DRzs were designed according to the sequences of S and C genes of HBV.The 10-23DRzs were connected to FSNPs,which constituted the FSNPs-DNA.The connecting efficiency and the protective effect of nanoparticles on 10-23DRzs were tested by sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE).The HepG2.2.15 cells were transfected by FSNPs-DNA,of which the inhibitory effects on HBsAg and HBeAg were analyzed by enzyme-linked immunosorbent assay(ELISA).Results The nanoparticles were spherical and uniform in size,with a diameter of 220 nm and the surface Zeta potential of +15.2 mV.The combination of DNA and FSNPs effectively protected DNA from nuclease degradation.Transfection of FSNPs-DNA significantly inhibited the expression of HBV S and C genes compared to the liposome control group.Conclusion The FSNPs have been successfully prepared and efficiently connected to HBV-specific 10-23DRzs,which significantly inhibit the expression of HBV S and C genes in cell culture.