AIM To evaluate the treatment effects of recombinant human interleukin-12(rh IL-12) on radiotherapy complications, such as severe myelosuppression or pancytopenia, the decline or imbalance of immune function, etc.METH...AIM To evaluate the treatment effects of recombinant human interleukin-12(rh IL-12) on radiotherapy complications, such as severe myelosuppression or pancytopenia, the decline or imbalance of immune function, etc.METHODS The patients received high-dose and short-course precise radiotherapy, such as Cyber knife and image-guided radiotherapy(IGRT), which can cause myelosuppression or pancytopenia and immune function decline within a short time. One-hundred subjects were enrolled in the study, and 50 were randomized to a treatment group which used rh IL-12 and 50 were randomized to a control group which used symptomatic and supportive therapy after radiotherapy. The 50 subjects in the treatment group were further divided into five subgroups and intervenedwith rh IL-12 at a dose of 50, 100, 150, 200 or 250 ng/kg respectively. The dose-effect relationship was observed. RESULTS Rh IL-12 significantly attenuated the decrease of peripheral blood cells in the treatment group, and immune function was improved after treatment. Due to the different radiation doses, there was a fluctuation within 12 h after treatment but mostly showing an increasing trend. As to the clinical manifestations, 2 patients in the 250 ng/kg subgroup showed low fever after administration, 1 patient in the 200 ng/kg subgroup and 2 patients in the 250 ng/kg subgroup showed mild impairment of liver function during the observation period.CONCLUSION Rh IL-12 has effective therapeutic and protective effects on complications following radiotherapy, such as the decline of blood cells, myelosuppression and the decline or imbalance of immune function, which indicated good prospects for development and application.展开更多
A 24-membered ring macrolide compound,macrolactin A has potential applications in pharmaceuticals for its anti-infectious and antiviral activity.In this study,macrolactin A was produced by a marine bacterium,which was...A 24-membered ring macrolide compound,macrolactin A has potential applications in pharmaceuticals for its anti-infectious and antiviral activity.In this study,macrolactin A was produced by a marine bacterium,which was identified as Bacillus subtilis by 16S ribosomal RNA(rRNA) sequence analysis.Electrospray ionization mass spectrometry(ESI/MS) and nuclear magnetic resonance(NMR) spectroscopy analyses were used to characterize this compound.To improve the production,response surface methodology(RSM) involving Box-Behnken design(BBD) was employed.Faeces bombycis,the main by-product in sericulture,was used as a nitrogen source in fermentation.The interactions between three significant factors,F.bombycis,soluble starch,and(NH4)2SO4 were investigated.A quadratic model was constructed to fit the production and the factors.Optimum medium composition was obtained by analysis of the model.When cultivated in the optimum medium,the production of macrolactin A was increased to 851 mg/L,2.7 times as compared to the original.This study is also useful to find another way in utilizing F.bombycis.展开更多
In order to establish a succession law of every phase in sintered Nd–Fe–B magnets marked as 38/38H/38SH/38 UH, four alloys of Nd33-xDyxAl0.7Nb0.6Cu0.1B1.05 Fe bal.(at%) were investigated after smelting processing, s...In order to establish a succession law of every phase in sintered Nd–Fe–B magnets marked as 38/38H/38SH/38 UH, four alloys of Nd33-xDyxAl0.7Nb0.6Cu0.1B1.05 Fe bal.(at%) were investigated after smelting processing, sintering processing, high-temperature tempering processing, and highand low-temperature tempering processing. It is found that the four phases: the Nd2Fe14B matrix phase, Nd-rich phase,B-rich phase, and defect phase can be inherited by means of the subsequent processing. These phases might have the special constitution and appearance in the different states.Magnetic properties also have succession law. In every processing except smelting one, the values of remanence and maximum energy product hardly alter, but the value of coercive force increases gradually.展开更多
文摘AIM To evaluate the treatment effects of recombinant human interleukin-12(rh IL-12) on radiotherapy complications, such as severe myelosuppression or pancytopenia, the decline or imbalance of immune function, etc.METHODS The patients received high-dose and short-course precise radiotherapy, such as Cyber knife and image-guided radiotherapy(IGRT), which can cause myelosuppression or pancytopenia and immune function decline within a short time. One-hundred subjects were enrolled in the study, and 50 were randomized to a treatment group which used rh IL-12 and 50 were randomized to a control group which used symptomatic and supportive therapy after radiotherapy. The 50 subjects in the treatment group were further divided into five subgroups and intervenedwith rh IL-12 at a dose of 50, 100, 150, 200 or 250 ng/kg respectively. The dose-effect relationship was observed. RESULTS Rh IL-12 significantly attenuated the decrease of peripheral blood cells in the treatment group, and immune function was improved after treatment. Due to the different radiation doses, there was a fluctuation within 12 h after treatment but mostly showing an increasing trend. As to the clinical manifestations, 2 patients in the 250 ng/kg subgroup showed low fever after administration, 1 patient in the 200 ng/kg subgroup and 2 patients in the 250 ng/kg subgroup showed mild impairment of liver function during the observation period.CONCLUSION Rh IL-12 has effective therapeutic and protective effects on complications following radiotherapy, such as the decline of blood cells, myelosuppression and the decline or imbalance of immune function, which indicated good prospects for development and application.
基金Project supported by the National Natural Science Foundation of China (No.20306026)the National Basic Research Program (973) of China (No.2007CB707805)
基金Project supported by the Science and Technology Department of Zhejiang Province,China(No.2009C33019)the IndustryUniversity-Research Institution Alliance for Microbial Medicine Technology Innovation and New Drug Development of China (No.2010ZX090401-403)the National Science and Technology Major Project of New Drug of China(Nos.2011ZX09201-101 and 2012ZX09103101-075)
文摘A 24-membered ring macrolide compound,macrolactin A has potential applications in pharmaceuticals for its anti-infectious and antiviral activity.In this study,macrolactin A was produced by a marine bacterium,which was identified as Bacillus subtilis by 16S ribosomal RNA(rRNA) sequence analysis.Electrospray ionization mass spectrometry(ESI/MS) and nuclear magnetic resonance(NMR) spectroscopy analyses were used to characterize this compound.To improve the production,response surface methodology(RSM) involving Box-Behnken design(BBD) was employed.Faeces bombycis,the main by-product in sericulture,was used as a nitrogen source in fermentation.The interactions between three significant factors,F.bombycis,soluble starch,and(NH4)2SO4 were investigated.A quadratic model was constructed to fit the production and the factors.Optimum medium composition was obtained by analysis of the model.When cultivated in the optimum medium,the production of macrolactin A was increased to 851 mg/L,2.7 times as compared to the original.This study is also useful to find another way in utilizing F.bombycis.
基金financially supported by the Key Project of Sichuan Provincial Science and Technology Department (Nos. 2011SZZ029, 2012SZZ025, and 2012JY0045)the Key Project of Sichuan Provincial Education Department (No.11ZA161)+1 种基金the Key Project of Mianyang Science and Technology Bureau (No. 12J005)the Youth Project of Sichuan Provincial Education Department (No. 11zb135)
文摘In order to establish a succession law of every phase in sintered Nd–Fe–B magnets marked as 38/38H/38SH/38 UH, four alloys of Nd33-xDyxAl0.7Nb0.6Cu0.1B1.05 Fe bal.(at%) were investigated after smelting processing, sintering processing, high-temperature tempering processing, and highand low-temperature tempering processing. It is found that the four phases: the Nd2Fe14B matrix phase, Nd-rich phase,B-rich phase, and defect phase can be inherited by means of the subsequent processing. These phases might have the special constitution and appearance in the different states.Magnetic properties also have succession law. In every processing except smelting one, the values of remanence and maximum energy product hardly alter, but the value of coercive force increases gradually.
