AIM:To improve the outcome of orthotopic transplantation in a mouse model,we used an absorbable gelatin sponge(AGS) in nude mice to establish an orthotopic implantation tumor model.METHODS:MHCC-97L hepatocellular carc...AIM:To improve the outcome of orthotopic transplantation in a mouse model,we used an absorbable gelatin sponge(AGS) in nude mice to establish an orthotopic implantation tumor model.METHODS:MHCC-97L hepatocellular carcinoma(HCC)cells stably expressing the luciferase gene were injected into the subcutaneous region of nude mice.One week later,the ectopic tumors were harvested and transplanted into the left liver lobe of nude mice.The AGS was used to establish the nude mouse orthotopic implantation tumor model.The tumor suppressor gene,paired box gene 5(PAX5),which is a tumor suppressor in HCC,was transfected into HCC cells to validate the model.Tumor growth was measured by bioluminescence imaging technology.Semi-quantitative reverse transcription polymerase chain reaction(RT-PCR) and histopathology were used to confirm the tumorigenicity of the implanted tumor from the MHCC-97L cell line.RESULTS:We successfully developed an orthotopic transplantation tumor model in nude mice with the use of an AGS.The success rate of tumor transplantation was improved from 60% in the control group to 100% in the experimental group using AGS.The detection of fluorescent signals showed that tumors grew in all live nude mice.The mice were divided into 3 groups:AGS-,AGS+/PAX5-and AGS+/PAX5 +.Tumor size was significantly smaller in PAX5 transfected nude mice compared to control mice(P < 0.0001).These fluorescent signal results were consistent with observations made during surgery.Pathologic examination further confirmed that the tissues from the ectopic tumor were HCC.Results from RT-PCR proved that the HCC originated from MHCC-97L cells.CONCLUSION:Using an AGS is a convenient and efficient way of establishing an indirect orthotopic liver transplantation tumor model with a high success rate.展开更多
To model the strain-inducedγ→α′phase transformation for the Cr-Mn metastable austenitic stainless steel,the 201Cu steel was chosen as the analytical material and the cylindrical samples of this steel with size ofϕ...To model the strain-inducedγ→α′phase transformation for the Cr-Mn metastable austenitic stainless steel,the 201Cu steel was chosen as the analytical material and the cylindrical samples of this steel with size ofϕ5 mm×10 mm were compressed at strains of 0.2–0.6 in the temperature range of 25–150°C and in the strain rate range of 0.1–5.0 s^(−1).The flaky samples were prepared by wire cutting from the cylindrical samples and the volume fraction of the strain-inducedα′phase was detected in the test point of the flaky samples.The volume fraction changing with the process parameters was modeled,and the critical temperatures and the critical strains to preventγ→α′phase transformation were calculated as other different process parameters changed.The linear fitting goodness of the model between the calculated volume fraction values and the tested ones is 0.986 and the validity of the model was verified by application in cold and warm rolling experiments.展开更多
基金Supported by National Natural Science Foundation of China, No.81201963Inner Mongolia Natural Science Foundation of China,No.2010MS1123
文摘AIM:To improve the outcome of orthotopic transplantation in a mouse model,we used an absorbable gelatin sponge(AGS) in nude mice to establish an orthotopic implantation tumor model.METHODS:MHCC-97L hepatocellular carcinoma(HCC)cells stably expressing the luciferase gene were injected into the subcutaneous region of nude mice.One week later,the ectopic tumors were harvested and transplanted into the left liver lobe of nude mice.The AGS was used to establish the nude mouse orthotopic implantation tumor model.The tumor suppressor gene,paired box gene 5(PAX5),which is a tumor suppressor in HCC,was transfected into HCC cells to validate the model.Tumor growth was measured by bioluminescence imaging technology.Semi-quantitative reverse transcription polymerase chain reaction(RT-PCR) and histopathology were used to confirm the tumorigenicity of the implanted tumor from the MHCC-97L cell line.RESULTS:We successfully developed an orthotopic transplantation tumor model in nude mice with the use of an AGS.The success rate of tumor transplantation was improved from 60% in the control group to 100% in the experimental group using AGS.The detection of fluorescent signals showed that tumors grew in all live nude mice.The mice were divided into 3 groups:AGS-,AGS+/PAX5-and AGS+/PAX5 +.Tumor size was significantly smaller in PAX5 transfected nude mice compared to control mice(P < 0.0001).These fluorescent signal results were consistent with observations made during surgery.Pathologic examination further confirmed that the tissues from the ectopic tumor were HCC.Results from RT-PCR proved that the HCC originated from MHCC-97L cells.CONCLUSION:Using an AGS is a convenient and efficient way of establishing an indirect orthotopic liver transplantation tumor model with a high success rate.
基金the financial support from the Open Project provided by the State Key Laboratory of Development and Application Technology of Automotive Steels(Baowu Steel Group)(Grant No.Y21ECEQ17Y).
文摘To model the strain-inducedγ→α′phase transformation for the Cr-Mn metastable austenitic stainless steel,the 201Cu steel was chosen as the analytical material and the cylindrical samples of this steel with size ofϕ5 mm×10 mm were compressed at strains of 0.2–0.6 in the temperature range of 25–150°C and in the strain rate range of 0.1–5.0 s^(−1).The flaky samples were prepared by wire cutting from the cylindrical samples and the volume fraction of the strain-inducedα′phase was detected in the test point of the flaky samples.The volume fraction changing with the process parameters was modeled,and the critical temperatures and the critical strains to preventγ→α′phase transformation were calculated as other different process parameters changed.The linear fitting goodness of the model between the calculated volume fraction values and the tested ones is 0.986 and the validity of the model was verified by application in cold and warm rolling experiments.
