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Dynamic tracking and mobility analysis of single GLUT4 storage vesicle in live 3T3-L1 cells 被引量:8
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作者 ChenHongLI libai +2 位作者 DongDongLI ShengXIA TaoXU 《Cell Research》 SCIE CAS CSCD 2004年第6期480-486,共7页
Glucose transporter 4 (GLUT4) is responsible for insulin-stimulated glucose transporting into the insulin-sensitive fat and muscle cells. The dynamics of GLUT4 storage vesicles (GSVs) remains to be explored and it is ... Glucose transporter 4 (GLUT4) is responsible for insulin-stimulated glucose transporting into the insulin-sensitive fat and muscle cells. The dynamics of GLUT4 storage vesicles (GSVs) remains to be explored and it is unclear how GSVs are arranged based on their mobility. We examined this issue in 3T3-L1 cells via investigating the three-dimensional mobility of single GSV labeled with EGFP-fused GLUT4. A thin layer of cytosol right adjacent to the plasma membrane was illuminated and successively imaged at 5 Hz under a total internal reflection fluorescence microscope with a penetration depth of 136 nm. Employing single particle tracking, the three-dimensional subpixel displacement of single GSV was tracked at a spatial precision of 22 nm. Both the mean square displacement and the diffusion coefficient were calculated for each vesicle. Tracking results revealed that vesicles moved as if restricted within a cage that has a mean radius of 160 nm, suggesting the presence of some intracellular tethering matrix. By constructing the histogram of the diffusion coefficients of GSVs, we observed a smooth distribution instead of the existence of distinct groups. The result indicates that GSVs are dynamically retained in a continuous and wide range of mobility rather than into separate classes. 展开更多
关键词 胰岛素 葡萄糖载体4 葡萄糖载体4贮藏囊泡 3T3-L1细胞 全内反射 荧光显微法 单质点跟踪 动力学
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Risk factors of early mortality and major morbidity in adult patients after surgical repair of partial atrioventricular septal defects
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作者 Shengwen Guo Yanbo Zhang +6 位作者 Xiaodong Lv Keming Yang Min Song Haibo Huang Yanhai Meng libai Tong Li 《中国循环杂志》 CSCD 北大核心 2018年第S01期148-148,共1页
Objective To investigate the risk factors for prolonged postoperative mechanical ventilation patients with atrioventricular septal defect(AVSD).Methods We retrospectively analyzed the clinical data of 76 patients with... Objective To investigate the risk factors for prolonged postoperative mechanical ventilation patients with atrioventricular septal defect(AVSD).Methods We retrospectively analyzed the clinical data of 76 patients with atrioventricular septal defect aged more than 18 years in our hospital from January 1^st 2011 to December 31^st 2017. 展开更多
关键词 atrioventricular SEPTAL DEFECT early MORTALITY MAJOR MORBIDITY
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Contemporary velocity field of crustal movement of Chinese mainland from Global Posi-tioning System measurements 被引量:29
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作者 NIUZhijun WANGMin +9 位作者 SUNHanrong SUNJianzhong YOUXinzhao GANWeijun XUEGuijiang HAOJinxin XINShaohua WANGYongqing WANGYongxiang libai 《Chinese Science Bulletin》 SCIE EI CAS 2005年第9期939-941,共3页
关键词 中国 大陆 地壳变形 测量方法 球形配置系统 地球科学
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