Objective:To determine the antioxidant activity of two edible marine seaweeds Eucheumacottonii and Padina sp..Methods:The two extraction methods such as conventional and soxhlet extraction were used to isolate the sec...Objective:To determine the antioxidant activity of two edible marine seaweeds Eucheumacottonii and Padina sp..Methods:The two extraction methods such as conventional and soxhlet extraction were used to isolate the secondary metabolites using methanol as a solvent medium.Total phenolic content of crude seaweeds extract were analysed by standard FC method.The different antioxidant assays DPPH,ferric reducing antioxidant power and β-carotene bleaching assays confirmed the antioxidant activities.Results:with expressed higher total phenolic content in the seaweeds extract.Also β-carotene bleaching assay lower activity compare with BHT as reference control.Additionally IR spectra showed the phenolic related functional groups are present in the solvent extract.The phenolic related compounds are mainly responsible for higher rate of antioxidant activity.Conclusions:DPPH and ferric reducing antioxidant power assays showed the positive correlation higher phenolic contents than the Eucheuma cottonii.And also the soxhlet extraction showed higher yield and better radical scavenging activity compared to conventional method.Moreover the studies confirmed both seaweeds are an effective candidate for the control the free radical scavenging activity.展开更多
基金Supported by Universiti Malaysia Pahang in the form of Internal Research Grant RDU No.120302.
文摘Objective:To determine the antioxidant activity of two edible marine seaweeds Eucheumacottonii and Padina sp..Methods:The two extraction methods such as conventional and soxhlet extraction were used to isolate the secondary metabolites using methanol as a solvent medium.Total phenolic content of crude seaweeds extract were analysed by standard FC method.The different antioxidant assays DPPH,ferric reducing antioxidant power and β-carotene bleaching assays confirmed the antioxidant activities.Results:with expressed higher total phenolic content in the seaweeds extract.Also β-carotene bleaching assay lower activity compare with BHT as reference control.Additionally IR spectra showed the phenolic related functional groups are present in the solvent extract.The phenolic related compounds are mainly responsible for higher rate of antioxidant activity.Conclusions:DPPH and ferric reducing antioxidant power assays showed the positive correlation higher phenolic contents than the Eucheuma cottonii.And also the soxhlet extraction showed higher yield and better radical scavenging activity compared to conventional method.Moreover the studies confirmed both seaweeds are an effective candidate for the control the free radical scavenging activity.
