背景:随着医学成像技术不断提高,人体解剖结构的可视化研究得到进一步发展,三维重建技术在医疗方面的作用也逐渐凸显。Mimics是目前应用最为广泛的医学图像三维重建软件,Arigin 3D Pro则是近期国内自主研发的一款基于3D打印的医学重构...背景:随着医学成像技术不断提高,人体解剖结构的可视化研究得到进一步发展,三维重建技术在医疗方面的作用也逐渐凸显。Mimics是目前应用最为广泛的医学图像三维重建软件,Arigin 3D Pro则是近期国内自主研发的一款基于3D打印的医学重构软件系统。目的:探讨Arigin 3D Pro软件和Mimics软件在医学图像三维重建处理中的精确度。方法:选取肝脏、脊柱、膝关节、心脏部位的图像数据,以Mimics软件重构后的3D模型为依据,进行两款软件重构模型的偏差分析;选取10例头颅部位及1例股骨粉碎性骨折的图像数据,进行三维重建,对每个重建模型均进行10组特征尺寸的测量,评估两款软件重构模型特征尺寸的差异。结果与结论:Arigin 3D Pro和Mimics软件对肝脏、脊柱、膝关节、心脏的三维重建模型,偏差的均值±标准差分别为(0.93±1.05),(0.36±0.74),(0.45±0.74)及(0.18±0.41)mm。Arigin 3D Pro和Mimics软件重建肝脏模型的时间分别为3 min、35 min,两款软件重建其他模型的时间均小于1 min。对于头颅、股骨粉碎性骨折的3D重构模型,两款软件Arigin 3D Pro软件与Mimics差异无统计学意义(P均>0.05)。说明Arigin 3D Pro软件与Mimics软件3D重构模型的精度相当,对于肝脏模型而言,Arigin 3D Pro软件的重建时间明显优于Mimics软件。展开更多
应用计算机检索PubMed数据库及Web of Science数据库,检索关于细胞定向排列方法的文章,中文检索词为"肌细胞排列,组织工程,表面结构约束,表面化学修饰,力学刺激,电磁刺激"。总结近年来关于组织工程肌细胞定向排列方法,对其进...应用计算机检索PubMed数据库及Web of Science数据库,检索关于细胞定向排列方法的文章,中文检索词为"肌细胞排列,组织工程,表面结构约束,表面化学修饰,力学刺激,电磁刺激"。总结近年来关于组织工程肌细胞定向排列方法,对其进行简要的综述,分析关键技术,探讨存在的问题并展望发展前景。肌细胞排列在细胞骨架重组、膜蛋白迁移、核基因表达和ECM重塑的过程中起到至关重要的作用。同时,对组织再生和调节组织的力学性能也有很大的影响。因此,细胞排列成为生物力学,细胞生物学,组织工程和再生医学应用中的关键技术。随着纳米和微制造等技术的发展,目前常用表面结构约束,表面化学修饰,外界刺激等方法,能有效诱导肌细胞定向排列,为进一步的细胞分化和形成肌小管提供条件。但是,目前的研究仍然存在一些不足,例如,马血清对细胞排列分化的促进机制仍不明确,仿生结构研究较少,极低频磁场对细胞排列的影响仍属空白等,是肌肉组织工程亟待解决的技术难点。最后,文章总结了在未来技术发展中,通过细胞精准打印,开发智能材料等新工艺,新材料控制细胞定向排列,不仅奠定了大尺寸三维支架制造和再生医学领域的理论基础,也是功能性肌肉组织的前瞻性研究方向,并将最终实现多层组织的集成,为临床应用提供研究基础。展开更多
BACKGROUND The results of previous animal experiments and clinical studies have shown that there is a correlation between expression of betatrophin and blood lipid levels.However,there are still differences studies on...BACKGROUND The results of previous animal experiments and clinical studies have shown that there is a correlation between expression of betatrophin and blood lipid levels.However,there are still differences studies on the correlation and interaction mechanism between betatrophin,angiogenin-likeprotein3(ANGPTL3)and lipoprotein lipase(LPL).In our previous studies,we found an increase in serum ANGPTL3 Levels in Chinese patients with coronary heart disease(CHD).Therefore,we retrospectively studied Kazakh CHD patients.AIM To explore the correlation between the betatrophin/ANGPTL3/LPL pathway and severity of coronary artery disease(CAD)in patients with CHD.METHODS Nondiabetic patients diagnosed with CHD were selected as the case group;79 were of Kazakh descent and 72 were of Han descent.The control groups comprised of 61 Kazakh and 65 Han individuals.The serum levels of betatrophin and LPL were detected by enzyme-linked immunosorbent assay(ELISA),and the double antibody sandwich ELISA was used to detect serum level of ANGPTL3.The levels of triglycerides,total cholesterol,and fasting blood glucose in each group were determined by an automatic biochemical analyzer.At the same time,the clinical baseline data of patients in each group were included.RESULTS Betatrophin,ANGPTL3 and LPL levels of Kazakh patients were significantly higher than those of Han patients(P=0.031,0.038,0.021 respectively).There was a positive correlation between the Gensini score and total cholesterol(TC),triglycerides(TG),low-density lipoprotein cholesterol(LDL-C),betatrophin,and LPL in Kazakh patients(r=0.204,0.453,0.352,0.471,and 0.382 respectively),(P=0.043,0.009,0.048,0.001,and P<0.001 respectively).A positive correlation was found between the Gensini score and body mass index(BMI),TC,TG,LDL-C,LPL,betatrophin in Han patients(r=0.438,0.195,0.296,0.357,0.328,and 0.446 respectively),(P=0.044,0.026,0.003,0.20,0.004,and P<0.001).TG and betatrophin were the risk factors of coronary artery disease in Kazakh patients,while BMI and betatrophin were the risk factors in Han patients.CONCLUSION There was a correlation between the betatrophin/ANGPTL3/LPL pathway and severity of CAD in patients with CHD.展开更多
文摘背景:随着医学成像技术不断提高,人体解剖结构的可视化研究得到进一步发展,三维重建技术在医疗方面的作用也逐渐凸显。Mimics是目前应用最为广泛的医学图像三维重建软件,Arigin 3D Pro则是近期国内自主研发的一款基于3D打印的医学重构软件系统。目的:探讨Arigin 3D Pro软件和Mimics软件在医学图像三维重建处理中的精确度。方法:选取肝脏、脊柱、膝关节、心脏部位的图像数据,以Mimics软件重构后的3D模型为依据,进行两款软件重构模型的偏差分析;选取10例头颅部位及1例股骨粉碎性骨折的图像数据,进行三维重建,对每个重建模型均进行10组特征尺寸的测量,评估两款软件重构模型特征尺寸的差异。结果与结论:Arigin 3D Pro和Mimics软件对肝脏、脊柱、膝关节、心脏的三维重建模型,偏差的均值±标准差分别为(0.93±1.05),(0.36±0.74),(0.45±0.74)及(0.18±0.41)mm。Arigin 3D Pro和Mimics软件重建肝脏模型的时间分别为3 min、35 min,两款软件重建其他模型的时间均小于1 min。对于头颅、股骨粉碎性骨折的3D重构模型,两款软件Arigin 3D Pro软件与Mimics差异无统计学意义(P均>0.05)。说明Arigin 3D Pro软件与Mimics软件3D重构模型的精度相当,对于肝脏模型而言,Arigin 3D Pro软件的重建时间明显优于Mimics软件。
文摘应用计算机检索PubMed数据库及Web of Science数据库,检索关于细胞定向排列方法的文章,中文检索词为"肌细胞排列,组织工程,表面结构约束,表面化学修饰,力学刺激,电磁刺激"。总结近年来关于组织工程肌细胞定向排列方法,对其进行简要的综述,分析关键技术,探讨存在的问题并展望发展前景。肌细胞排列在细胞骨架重组、膜蛋白迁移、核基因表达和ECM重塑的过程中起到至关重要的作用。同时,对组织再生和调节组织的力学性能也有很大的影响。因此,细胞排列成为生物力学,细胞生物学,组织工程和再生医学应用中的关键技术。随着纳米和微制造等技术的发展,目前常用表面结构约束,表面化学修饰,外界刺激等方法,能有效诱导肌细胞定向排列,为进一步的细胞分化和形成肌小管提供条件。但是,目前的研究仍然存在一些不足,例如,马血清对细胞排列分化的促进机制仍不明确,仿生结构研究较少,极低频磁场对细胞排列的影响仍属空白等,是肌肉组织工程亟待解决的技术难点。最后,文章总结了在未来技术发展中,通过细胞精准打印,开发智能材料等新工艺,新材料控制细胞定向排列,不仅奠定了大尺寸三维支架制造和再生医学领域的理论基础,也是功能性肌肉组织的前瞻性研究方向,并将最终实现多层组织的集成,为临床应用提供研究基础。
基金Supported by National Natural Science Foundation of China,No.8660085Natural Science Foundation of Shihezi University,No.ZZZC201712A
文摘BACKGROUND The results of previous animal experiments and clinical studies have shown that there is a correlation between expression of betatrophin and blood lipid levels.However,there are still differences studies on the correlation and interaction mechanism between betatrophin,angiogenin-likeprotein3(ANGPTL3)and lipoprotein lipase(LPL).In our previous studies,we found an increase in serum ANGPTL3 Levels in Chinese patients with coronary heart disease(CHD).Therefore,we retrospectively studied Kazakh CHD patients.AIM To explore the correlation between the betatrophin/ANGPTL3/LPL pathway and severity of coronary artery disease(CAD)in patients with CHD.METHODS Nondiabetic patients diagnosed with CHD were selected as the case group;79 were of Kazakh descent and 72 were of Han descent.The control groups comprised of 61 Kazakh and 65 Han individuals.The serum levels of betatrophin and LPL were detected by enzyme-linked immunosorbent assay(ELISA),and the double antibody sandwich ELISA was used to detect serum level of ANGPTL3.The levels of triglycerides,total cholesterol,and fasting blood glucose in each group were determined by an automatic biochemical analyzer.At the same time,the clinical baseline data of patients in each group were included.RESULTS Betatrophin,ANGPTL3 and LPL levels of Kazakh patients were significantly higher than those of Han patients(P=0.031,0.038,0.021 respectively).There was a positive correlation between the Gensini score and total cholesterol(TC),triglycerides(TG),low-density lipoprotein cholesterol(LDL-C),betatrophin,and LPL in Kazakh patients(r=0.204,0.453,0.352,0.471,and 0.382 respectively),(P=0.043,0.009,0.048,0.001,and P<0.001 respectively).A positive correlation was found between the Gensini score and body mass index(BMI),TC,TG,LDL-C,LPL,betatrophin in Han patients(r=0.438,0.195,0.296,0.357,0.328,and 0.446 respectively),(P=0.044,0.026,0.003,0.20,0.004,and P<0.001).TG and betatrophin were the risk factors of coronary artery disease in Kazakh patients,while BMI and betatrophin were the risk factors in Han patients.CONCLUSION There was a correlation between the betatrophin/ANGPTL3/LPL pathway and severity of CAD in patients with CHD.
