A monolithic integrated two-section distributed feedback(TS-DFB)semiconductor laser for high-speed direct modulation is proposed and analyzed theoretically.The grating structure of the TS-DFB laser is designed by the ...A monolithic integrated two-section distributed feedback(TS-DFB)semiconductor laser for high-speed direct modulation is proposed and analyzed theoretically.The grating structure of the TS-DFB laser is designed by the reconstructionequivalent-chirp(REC)technique,which can reduce the manufacturing cost and difficulty,and achieve high wavelength controlling accuracy.The detuned loading effect and the photon-photon resonance(PPR)effect are utilized to enhance the modulation bandwidth of the TS-DFB laser,exceeding 37 GHz,while that of the conventional one-section DFB laser is only 16 GHz.When the bit rate of the non-return-to-zero(NRZ)signal reaches 55 Gb/s,a clear eye diagram with large opening can still be obtained.These results show that the proposed method can enhance the modulation bandwidth of DFB laser significantly.展开更多
Pancreatic endocrine islets are vital for glucose homeostasis. However, the islet developmental trajectory and its regulatory network are not well understood. To define the features of these specification and differen...Pancreatic endocrine islets are vital for glucose homeostasis. However, the islet developmental trajectory and its regulatory network are not well understood. To define the features of these specification and differentiation processes, we isolated individual islet cells from TgBAC(neurod1:EGFP) transgenic zebrafish and analyzed islet developmental dynamics across four different embryonic stages using a single-cell RNA-seq strategy. We identified proliferative endocrine progenitors, which could be further categorized by different cell cycle phases with the G1/S subpopulation displaying a distinct differentiation potential. We identified endocrine precursors, a heterogeneous intermediate-state population consisting of lineage-primed alpha, beta and delta cells that were characterized by the expression of lineage-specific transcription factors and relatively low expression of terminally differentiation markers. The terminally differentiated alpha, beta, and delta cells displayed stage-dependent differentiation states, which were related to their functional maturation. Our data unveiled distinct states, events and molecular features during the islet developmental transition, and provided resources to comprehensively understand the lineage hierarchy of islet development at the single-cell level.展开更多
基金the National Key Research and Development Program of China(Grant No.2020YFB2205804)the National Natural Science Foundation of China(Grant Nos.61974165 and Grant 61975075)the National Natural Science Foundation of China for the Youth,China(Grant No.62004105)。
文摘A monolithic integrated two-section distributed feedback(TS-DFB)semiconductor laser for high-speed direct modulation is proposed and analyzed theoretically.The grating structure of the TS-DFB laser is designed by the reconstructionequivalent-chirp(REC)technique,which can reduce the manufacturing cost and difficulty,and achieve high wavelength controlling accuracy.The detuned loading effect and the photon-photon resonance(PPR)effect are utilized to enhance the modulation bandwidth of the TS-DFB laser,exceeding 37 GHz,while that of the conventional one-section DFB laser is only 16 GHz.When the bit rate of the non-return-to-zero(NRZ)signal reaches 55 Gb/s,a clear eye diagram with large opening can still be obtained.These results show that the proposed method can enhance the modulation bandwidth of DFB laser significantly.
基金the National Key Basic Research Program of China (2015CB942800)the National Key Research and Development Program of China, Stem Cell and Translational Research (2016YFA0100500)the National Natural Science Foundation of China (NSFC)(3187145 31671500, 31730060, and 81371264).
文摘Pancreatic endocrine islets are vital for glucose homeostasis. However, the islet developmental trajectory and its regulatory network are not well understood. To define the features of these specification and differentiation processes, we isolated individual islet cells from TgBAC(neurod1:EGFP) transgenic zebrafish and analyzed islet developmental dynamics across four different embryonic stages using a single-cell RNA-seq strategy. We identified proliferative endocrine progenitors, which could be further categorized by different cell cycle phases with the G1/S subpopulation displaying a distinct differentiation potential. We identified endocrine precursors, a heterogeneous intermediate-state population consisting of lineage-primed alpha, beta and delta cells that were characterized by the expression of lineage-specific transcription factors and relatively low expression of terminally differentiation markers. The terminally differentiated alpha, beta, and delta cells displayed stage-dependent differentiation states, which were related to their functional maturation. Our data unveiled distinct states, events and molecular features during the islet developmental transition, and provided resources to comprehensively understand the lineage hierarchy of islet development at the single-cell level.
