穿心莲内酯磺化物注射液解热镇痛抗炎作用研究

为探究穿心莲内酯磺化物注射液的解热、镇痛、抗炎作用,将SD大鼠和KM小鼠分别随机分为空白组,阳性药穿心莲注射液组,穿心莲内酯磺化物注射液高、中、低剂量组。通过皮下注射干酵母诱导大鼠发热模型探究其解热作用;通过冰醋酸致小鼠扭体法探究其镇痛作用;通过二甲苯诱导小鼠耳肿胀法及λ-角叉菜胶诱导大鼠足肿胀法探究其抗炎作用。结果表明,穿心莲内酯磺化物注射液可显著抑制干酵母所致发热大鼠的肛温上升;显著抑制冰醋酸所致小鼠疼痛,减少扭体次数;显著降低二甲苯致小鼠耳肿胀,减少肿胀度;显著抑制λ-角叉菜胶致大鼠足肿胀,减少肿胀度。由此可知,穿心莲内酯磺化物注射液具有明显的解热、镇痛、抗炎的作用。

岭南地区高校体育馆绿色技术研究综述

在体育全面发展的公共政策背景下,体育社会化成为现阶段体育事业发展的核心,学校体育作为连接竞技体育与群众体育的桥梁,其建筑、人与运动环境之间的关系已经成为了该领域研究的重心。岭南地区高校体育馆的发展源于竞技体育的推动,社会办体育与赛训兼顾的社会化背景给高校体育馆的建设提供了机遇与方向。现阶段的岭南地区高校体育馆绿色技术研究,以华南理工大学亚热带建筑科学重点实验室为依托,自然通风作为主要研究方向,热环境与光环境等各项绿色技术均有一定的成果体现。随着近年来实验条件的提高,单一技术研究逐步由普适化向精细化过渡,并呈现出多技术协同研究的耦合化发展趋势。

Lentivirus-Mediated Short Hairpin RNA for Follistatin Downregulation Suppresses Tumor Progression in Hypopharyngeal Carcinoma

Objective:Follistatin(FST)inhibits the action of activin by interfering with the binding of activin to its receptor.Although the prognostic value of FST in various cancers has been investigated previously,studies rarely focused on hypopharyngeal carcinoma(HPC).In our study,the effect of FST expression on HPC tissues and cell lines was investigated.Methods:A total of 60 patients with HPC were recruited for this study.Levels of FST mRNA and protein were measured by quantitative polymerase chain reaction(PCR)and immunohistochemistry in HPC tissue samples and by qPCR in the HPC FaDu cells,as well as immortal nasopharyngeal epithelial cell line NP-69 cells.After silencing the FST expression in FaDu cells using lentivirus-mediated siRNA that was specific for FST mRNA,cell proliferation was determined by a Celigo assay.Tumor growth was monitored in nude mice and viability was determined by a methylthiazoletetrazolium assay.The ratio of cell cycle arrest and apoptosis was evaluated by flow cytometry.The colony formation ability was performed using Giemsa staining.In addition,wound healing and Transwell migration and invasion assays were performed for the analysis of cell motility.Results:FST expression was significantly higher in human HPC tissue and FaDu cells than in normal tissue and NP-69 cells.A higher expression of FST in HPC samples was positively correlated with advanced tumors.Moreover,FST knockdown by shRNA significantly decreased cell growth,colony formation,migration and invasion.Furthermore,FST silencing increased the cell apoptosis percentage,and arrested cell cycle in the S phase in FaDu cells.In addition,FST silencing suppressed tumor growth in vivo.Conclusions:Our results indicated that the FST gene was associated with HPC progression and may serve as a potential therapeutic target for the treatment of HPC.

Presentation of Boerhaave's syndrome as an upper-esophageal perforation associated with a right-sided pleural effusion:A case report

BACKGROUND Spontaneous esophageal rupture or Boerhaave’s syndrome is a rare and acute disease with a high incidence of misdiagnosis and mortality.Here,we aimed to explore the clinical characteristics,diagnosis,treatment,and prognosis of spontaneous esophageal rupture,and to analyze the causes of misdiagnosis during the treatment of spontaneous esophageal rupture.CASE SUMMARY The clinical features of the patient with spontaneous esophageal rupture misdiagnosed earlier as pleural effusion were retrospectively analyzed and the reasons for misdiagnosis are discussed based on a current review of the literature.The patient was admitted to a local hospital due to shortness of breath accompanied by vomiting and abdominal distension for five hours.Based on the computed tomography(CT)scan analysis,clinically,right pleural effusion was diagnosed.However,the patient was unwilling to undergo right closed thoracic drainage.The patient also had intermittent fevers against infection,and during the course of treatment,he complained of chest pain,following which,he was transferred to our hospital.Grapefruit-like residue drainage fluid was observed.Re-examination of the chest CT scans suggested the presence of spontaneous perforation in the upper left esophagus.Therefore,the patient underwent an urgent esophageal hiatus repair.Unfortunately,the patient died of infection and respiratory failure due to progressive dyspnea after surgery.CONCLUSION Spontaneous esophageal rupture is a rare disease associated with high fatality.The patients do not present typical clinical symptoms and the disease progresses rapidly.This case report highlights the importance of a dynamic review of chest CT scan,not only for the initial identification of segmental injury but also for prioritizing subsequent treatment strategies.Moreover,we have presented some clues for clinicians to recognize and diagnose spontaneous esophageal rupture at rare sites(upper-esophageal segment)through this case report of spontaneous esophageal rupture that caused the patient’s death.We have also summarized the reasons for the misdiagnosis and lessons learned.

Onyx embolization of a spinal epidural hemorrhage caused by thoracic spinal epidural arteriovenous fistula:A case report and literature review

Spinal epidural hemorrhages(SEDH)caused by spinal epidural arteriovenous fistulas(SEAVFs)are rare;thus,their specific pathogenesis has not been explained.Furthermore,the standard treatment for SEAVFs has not yet been defined.Here we report the case of a 36-year-old Chinese man who experienced acute onset chest pain and tightness.His symptoms rapidly aggravated until the lower limbs were unable to support him.Spinal magnetic resonance angiography(MRA)revealed a localized SEAVF and a secondary spinal cord lesion at the T4 level.Digital subtraction angiography(DSA)confirmed the presence of the SEDH/SEAVF at the T3–4 level with the left radicular artery feeding the fistula.Based on DSA and MRA findings,SEDH,local spinal cord infarction,and spinal venous reflux disorder were conditionally diagnosed.Using the arterial route,Onyx-34 was injected into the fistula to embolize the feeding arteries and the venous system.Angiography was performed after the microcatheter was withdrawn,and no residual fistula or anterior spinal artery was observed.The six-week follow-up MRI showed acceptable healing of the SEAVF,and the patient improved neurologically.This case suggests that endovascular treatment with Onyx-34 embolization should be considered a promising treatment strategy for this type of complicated SEAVF.

A general observatory control software framework design for existing small and mid-size telescopes

A general framework for observatory control software would help to improve the efficiency of observation and operation of telescopes, and would also be advantageous for remote and joint observations. We describe a general framework for observatory control software, which considers principles of flexibility and inheritance to meet the expectations from observers and technical personnel. This framework includes observation scheduling, device control and data storage. The design is based on a finite state machine that controls the whole process.

液氢中空气溶解度预测及氮—氧存在模式研究

为探析空气在液氢中的溶解极限及氮氧分子的存在模式,采用正规溶液模型(SH模型)和改进的正规溶液模型(MSH模型)对空气在液氢中的溶解规律开展了理论预示。为解决溶解度参数在靠近临界温度附近取值不合理的问题,提出一种新型溶解度参数求解方法,实现了SH模型在18.0~32.5 K液氢温区空气溶解规律的准确预测。基于实验数据的对比修正,提出了适用于预测液氢中气体溶解规律的MSH模型。结果发现:氮气、氧气、二氧化碳、水在液氢中的溶解度分别为10^(-8)、10^(-12)、10^(-51)、10^(-89)摩尔分数量级,差异极大。空气在液氢中的溶解规律与存在模式随着空气量的增大而存在3种不同形态:完全溶解的氮、氧分子;氮气完全溶解而部分氧分子结晶析出;氮、氧均部分结晶析出,且固空颗粒中的氮氧比例逐渐接近空气中比例。研究应可为液氢系统的安全设计提供理论指导。

基于液质联用技术和网络药理学的附子理中汤干预溃疡性结肠炎的活性成分和作用机制研究

目的基于高分辨质谱仪、靶向定量代谢组学技术和网络药理学探讨附子理中汤(FZLZD)治疗溃疡性结肠炎(UC)的活性成分及作用机制。方法首先,通过高分辨液质联用仪对附子理中汤进行定性检测,将其测定的成分进行验证并结合有关文献报道筛选出可能活性化合物;然后利用靶向定量代谢组学技术、结合多元统计分析方法,对筛选出的化合物进行靶向定量;最后运用网络药理学预测附子理中汤治疗溃疡性结肠炎可能的机制。结果本研究共获得苯甲酰新乌头原碱、苯甲酰乌头原碱、白术内酯Ⅰ、白术内酯Ⅱ、白术内酯Ⅲ、党参炔苷、8-姜酚、10-姜酚、甘草苷、甘草素、甘草酸、6-姜烯酚、苯甲酰次乌头原碱、乌头原碱、6-姜酚、次乌头碱、腺苷、异甘草苷、异甘草素和甘草次酸20个活性成分,各不同来源的附子和甘草的活性成分含量差异较大,附子理中汤治疗溃疡性结肠炎与癌症通路、癌症中的蛋白聚糖、EGFR酪氨酸激酶抑制剂耐药性、AGE-RAGE信号通路,以及IL-6、IL-8等炎症通路有关。结论虽然各来源都选用合格中药,但质量标志物的含量范围会影响其临床效果;将附子、甘草含量差异较大的活性成分:甘草酸、异甘草素、甘草素、苯甲酰次乌头原碱、甘草苷、甘草次酸作为主要筛选中的Q-marker;此研究有助于对中药不同来源及其主要活性成分含量进行质量控制,对中药-疾病可能的通路靶点进行预测,为后续进一步的药理作用及机制研究提供依据。

产琥珀酸放线杆菌利用甘薯粉产丁二酸发酵条件优化

为了优化产琥珀酸放线杆菌(Actinobacillussuccinogenes)发酵甘薯粉生产丁二酸的发酵培养基,提高丁二酸产量,降低生产成本,本研究首先通过单因素实验对甘薯粉发酵产丁二酸的甘薯粉、MgCO_(3)、液化酶、糖化酶、氮源浓度和发酵时间进行了优化,再利用正交试验确定重要参数的最佳水平,最后利用2 L发酵罐对获得最佳发酵工艺进行放大实验。结果表明,混合氮源(酵母粉:玉米浆=1:2)可满足甘薯粉丁二酸发酵营养需求,影响丁二酸产量的重要参数是甘薯粉、MgCO_(3)、液化酶、糖化酶、混合氮源(酵母粉:玉米浆=1:2)的浓度。各因素最佳水平为:甘薯粉115 g/L、MgCO_(3)60 g/L,液化酶0.008 KUN-S/g底物,糖化酶3.09 AGU/g底物,混合氮源33 g/L。优化后丁二酸产量可达69.89 g/L,与优化前相比(42.46 g/L),丁二酸浓度提高了64.6%。2 L发酵罐发酵72 h,丁二酸可达71.42 g/L,丁二酸产率为79.87%,生产强度为0.99 g/(L·h)。因此,利用A.succinogenes发酵甘薯粉产丁二酸具有较好的工业化应用前景。

Multimodal MRI diagnosis and transvenous embolization of a basicranial emissary vein dural arteriovenous fistula:A case report

A dural arteriovenous fistula(DAVF) is an abnormal linkage connecting the arterial and venous systems within the intracranial dura mater. A basicranial emissary vein DAVF drains into the cavernous sinus and the ophthalmic vein, similar to a cavernous sinus DAVF. Precise preoperative identification of the DAVF location is a prerequisite for appropriate treatment. Treatment options include microsurgical disconnection, endovascular transarterial embolization(TAE), transvenous embolization(TVE), or a combination thereof. TVE is an increasingly popular approach for the treatment of DAVFs and the preferred approach for skull base locations, due to the risk of cranial neuropathy caused by dangerous anastomosis from arterial approaches. Multimodal magnetic resonance imaging(MRI) can provide anatomical and hemodynamic information for TVE. The therapeutic target must be precisely embolized in the emissary vein, which requires guidance via multimodal MRI. Here, we report a rare case of successful TVE for a basicranial emissary vein DAVF, utilizing multimodal MRI assistance. The fistula had vanished, pterygoid plexus drainage had improved, and the inferior petrosal sinus had recanalized, as observed on 8-month follow-up angiography. Symptoms and signs of double vision, caused by abduction deficiency, disappeared. Detailed anatomic and hemodynamic assessment by multimodal MRI is the key to guiding successful diagnosis and treatment.

孤独症谱系障碍患儿神经免疫的改变及其与智力水平的关系

目的探讨孤独症谱系障碍(ASD)患儿神经免疫的改变及其与智力水平的关系。方法选取2018年11月至2021年10月徐州市儿童医院收治的112名ASD患儿为观察组,另选取同期在院体检的112名正常儿童为对照组。采用酶联免疫吸附试验方法检测两组的血清神经免疫指标[白介素6(IL-6)、白介素17A(IL-17A)、抗髓鞘碱性蛋白抗体(anti-MBP)]的表达水平;应用Griffiths发育评估量表中文版(GDS-C)评估两组儿童的智力水平,并采用Pearson相关性分析法分析ASD患儿神经免疫指标水平与智力水平的关系。结果观察组血清IL-6、IL-17A、anti-MBP的水平均显著高于对照组(t值分别为10.826、10.878、24.060,P<0.05);观察组GDS-C量表粗大运动、个人社会、听力语言、手眼协调、视觉表现和实际推理6个维度发育商及总发育商均显著低于对照组(t值分别为14.735、36.969、30.631、29.943、23.932、9.438、20.644,P<0.05)。经Pearson相关性分析显示,ASD患儿血清IL-6、IL-17A、anti-MBP的水平与GDS-C量表的6个维度发育商及总发育商均呈显著负相关(r=-0.731~-0.496,P<0.05)。结论ASD患儿存在明显的神经免疫紊乱和智力水平下降,且神经免疫指标与智力水平呈负相关。

响应面试验优化产琥珀酸放线杆菌GXAS137发酵粗甘油产丁二酸工艺

通过优化产琥珀酸放线杆菌GXAS137发酵粗甘油产丁二酸的培养基,提高丁二酸产量,降低生产成本。先通过单因素试验对粗甘油发酵产丁二酸的电子受体、初始粗甘油质量浓度及氮源进行了优化,再利用响应面试验确定重要参数的最佳水平。结果表明:二甲基亚砜(dimethyl sulfoxide,DMSO)为最适电子受体,玉米浆可替换酵母粉作为氮源,各因素的最佳条件为:初始粗甘油质量浓度55.43 g/L、DMSO质量浓度10.35 g/L、玉米浆质量浓度17.69 g/L。优化后丁二酸产量达到37.02 g/L,丁二酸得率为66.79%,生产强度为0.51 g/(L·h)。与初始条件下丁二酸产量(16.88 g/L)相比,优化后提高了1.19倍。本研究为微生物发酵粗甘油原料生产丁二酸提供了理论支持。

一项人流量模型参数估计方法的比较研究

智慧城市和大数据技术的兴起,为工作生活带来了诸多便利。人流量分析作为智慧城市的重要经济分析手段,受到了越来越多学者的关注。利用基于注意力(attention)机制的视觉变换(vision transformer,Vi T)图像分类算法对监控进行人像采集,从得到的视频中获取人流量数据,在切比雪夫(Chebyshev)基函数张成的函数空间中,通过将原来的人流量数据由分段的Chebyshev基函数的线性组合,构造了分段的Chebyshev多项式表示,研究并讨论了它们的一些参数估计方法。所提出的方法有效地降低了人流量表示的最大误差,提高了人流量大数据分析方法的精确度。

硅-芳键的断裂反应

利用等摩尔竞争法我们研究了五种芳基三甲基硅烷与氯化氢的非均相断裂反应。用气体色谱法对各竞争反应产物进行定量分析,求出各断裂产物的相对性产率,结果表明其活性顺序是与相应的均相断裂反应相一致的。

川续断提取物对氟苯尼考在鸡体内的药物动力学影响

为研究川续断提取物对氟苯尼考在鸡体内药物动力学影响,将30只肉鸡(2.0 kg±0.1 kg)随机分成2组,每组15只。试验组连续7 d分别灌服川续断提取物(0.3 g/kg,1次/d),对照组给予相同体积的生理盐水,第8天2个组各10只鸡灌服氟苯尼考(30 mg/kg)后按时间点连续采血,采用高效液相色谱法检测氟苯尼考血药浓度,数据采用DAS2.0进行分析。各组剩下的5只鸡,处死鸡剖检取肝脏和空肠,荧光定量PCR检测组织中CYP3A37、MDR1和CXR mRNA表达。试验组和对照组的氟苯尼考主要药物动力学参数为:C_(max)分别为20.829 mg/L±5.267 mg/L、18.429 mg/L±8.365 mg/L,T_(max)分别为0.688 h±0.139 h、1.250 h±0.141 h,AUC_(0-∞)分别为57.907 mg/L·h±8.12 mg/L·h、47.729 mg/L·h±11.601 mg/L·h,T_(1/2)分别为3.649 h±0.193 h、2.520 h±0.255 h,MRT分别为3.885 h±0.487 h、3.151 h±1.604 h,Vd分别为1.338 L/kg±0.492 L/kg、2.139 L/kg±1.182 L/kg,CL分别为0.426 L/h·kg±0.071 L/h·kg、0.657L/h·kg±0.148 L/h·kg。川续断提取物的连续使用,引起了氟苯尼考药物动力学特征明显改变,AUC_(0-∞)和T_(1/2)增加(P<0.05),T_(max)和CL降低(P<0.05)。荧光定量PCR检测结果显示,川续断提取物给药组抑制了CXR和CYP3A37在肉鸡肝脏和空肠中mRNA的表达量(P<0.05)。结果表明,川续断提取物连续给药7 d后,增加了氟苯尼考在鸡体内的吸收速度,延缓了代谢,提示两者在临床上合用有潜在的增效作用。

酿酒酵母pdc基因缺陷菌株的构建及其丙酮酸发酵特性

丙酮酸是一种重要的有机酸,在聚合物、化妆品、食品添加剂、医药等领域具有广泛应用。酿酒酵母被认为是丙酮酸生产的潜在最适微生物,但其糖酵解过程产生的丙酮酸会在胞质内的丙酮酸脱羧酶催化作用下降解为CO2和乙醛,造成碳代谢流的损失。为将更多的碳代谢流引向丙酮酸合成,该研究通过敲除丙酮酸脱羧酶的3个结构基因(pdc1、pdc5和pdc6),显著地促进了丙酮酸积累,但也造成突变菌株XY-156生长缓慢。与进化之前相比,采用适应性进化策略驯化得到的菌株XY-156A,在细胞生长、葡萄糖消耗以及丙酮酸积累方面均获得显著提高;进一步利用2 L发酵罐进行批次补料发酵试验,发酵76 h丙酮酸产量可达105 g/L,生产强度为1.38g/(L·h),得率为0.5 g/g葡萄糖。结果表明,采用失活丙酮酸脱羧酶与适应性定向进化相结合的策略改造酿酒酵母,能够实现其高效积累丙酮酸,可为生物基丙酮酸工业化生产奠定坚实的基础。

糖尿病视网膜病变筛查中的眼底图像质量控制

目的糖尿病性视网膜病变(diabetic retinopathy,DR,以下简称糖网病)筛查中,有相当比例的图像因聚焦不清或曝光不佳不可用于临床诊断,浪费了医疗资源,因而,有必要对眼底图像进行质量监控。本文提出一种基于小波变换和最小二乘支持向量机(least square support vector machine,LS-SVM)的眼底图像质量控制算法。方法首先,对眼底图像进行2层静态小波变换,计算8个子图像的能量值作为特征向量,再利用LS-SVM对眼底图像进行质量评判。本文将中国人民解放军火箭军特色医学中心眼科提供的146幅图像,分为训练集和测试集,LS-SVM使用训练集进行学习后,对测试集的97幅图像进行分类测试。结果训练后的LS-SVM能够对测试集很好地分类,鉴别出模糊的眼底图像。以线性函数为核的LS-SVM分类正确率为100%,以高斯径向基函数为核的LS-SVM的分类正确率为97. 9%。结论以2层静态小波分解子图像的能量值为输入特征向量的LS-SVM能够很好地鉴别出本文使用的眼底图像是否模糊。

川续断皂苷乙对氟苯尼考在大鼠体内的药动学影响

研究川续断皂苷乙对氟苯尼考在大鼠体内药动学影响。将24只大鼠(200±10)g随机分成两组,每组12只。试验组连续7 d灌胃川续断皂苷乙(60 mg/kg,1次/d),对照组给予相同体积的生理盐水,第8天两组各6只大鼠灌服氟苯尼考(30 mg/kg)后按时间点连续采血,采用高效液相色谱法检测氟苯尼考血药浓度,数据采用DAS2.0进行分析。处死各组剩下的6只,解剖取肝脏和空肠,荧光定量PCR法和免疫印迹法分别检测组织中CYP1A2、CYP2C11、CYP3A1、MDR1的mRNA表达水平和蛋白表达水平。结果表明,试验组AUC_(0-∞)、MRT_(0-∞)、T_(1/2)和C_(max)与对照组相比显著增加(P<0.05),CL与对照组相比显著降低(P<0.05);试验组CYP1A2、CYP2C11在肝脏的mRNA表达水平和MDR1在空肠的mRNA表达水平与对照组相比显著降低(P<0.05);试验组大鼠肝脏中CYP1A2和CYP2C11酶表达量以及空肠中P-糖蛋白表达量与对照组相比显著降低(P<0.05)。川续断皂苷乙连续给药7 d,增加了氟苯尼考在大鼠体内的吸收程度,同时延缓了代谢,可能与川续断皂苷抑制了CYP1A2酶和CYP2C11酶在肝脏的表达和P-糖蛋白在空肠的表达有关。

基于转座子策略提高链霉菌中landomycin E产量的研究

【目的】对影响放线菌链霉菌Streptomyces globisporus产landomycin E(laE)的代谢网络进行研究,以提高次生代谢物的产量。【方法】通过构建含强启动子和抗性标记的转座子Tn7为基础的转座子,整合至S.globisporus的染色体产生突变库,筛选高产量的突变株并对其代谢网络进行研究分析。【结果】利用构建好的Tn7-转座子连续转化链霉菌S.globisporus,经过数轮的突变和筛选,得到6株产量有较大改变的突变株,对整合位点的亚克隆和测序结果表明,该位点整合导致编码类似细菌的某些调节因子如TetR和GntR家族的蛋白的基因失活。【结论】所构建的经过修饰的微型Tn7-转座子不仅带有抗性标记且有启动子,可插入链霉菌染色体产生突变,进而提高次生代谢物laE的产量,同时也证明,转座子基载体可应用于非模式菌链霉菌。

母亲相关因素与新生儿先天性甲状腺功能减低症易感性的病例对照研究

目的研究母亲相关因素与新生儿先天性甲状腺功能减低症(CH)易感性的关联。方法采用病例-对照研究设计,以2016年1月1日至2017年12月31日在河南省新生儿疾病筛查中心确诊的CH新生儿作为病例,按照1:1比例匹配同性别和出生日期相近的健康新生儿作为对照。采用条件logistic回归及相加相乘交互作用分析评估新生儿CH易感的风险因素。结果该期间共筛查新生儿2771661例,确诊CH 1494例,粗发病率53.9/10万。完成电话问卷调查且数据合格的CH患儿、对照共843对。条件logistic回归分析显示,母亲生育年龄大、学历低、居住在农村、甲状腺疾病家族史、孕期甲醛接触史、孕期射线接触史、孕期用药史是新生儿CH发生的风险因素(均P<0.05),而母亲年龄低、孕期使用黄体酮类保胎药是新生儿CH的保护因素(均P<0.05)。结论母亲生育年龄大、学历低、居住在农村、甲状腺疾病家族史、孕期甲醛接触史、孕期射线接触史及孕期用药史可增加新生儿CH的易感性。