Fluorescence imaging in the second near-infrared window(NIR-II,900–1880 nm)with less scattering background in biological tissues has been combined with the confocal microscopic system for achieving deep in vivo imagi...Fluorescence imaging in the second near-infrared window(NIR-II,900–1880 nm)with less scattering background in biological tissues has been combined with the confocal microscopic system for achieving deep in vivo imaging with high spatial resolution.However,the traditional NIR-IIfluorescence confocal microscope with separate excitation focus and detection pinhole makes it possess low confocal e±ciency,as well as di±cultly to adjust.Two types of upgraded NIR-IIfluorescence confocal microscopes,sharing the same pinhole by excitation and emission focus,leading to higher confocal e±ciency,are built in this work.One type is-ber-pinhole-based confocal microscope applicable to CW laser excitation.It is constructed forfluorescence intensity imaging with large depth,high stabilization and low cost,which could replace multiphotonfluorescence microscopy in some applications(e.g.,cerebrovascular and hepatocellular imaging).The other type is air-pinhole-based confocal microscope applicable to femtosecond(fs)laser excitation.It can be employed not only for NIR-IIfluorescence intensity imaging,but also for multi-channelfluorescence lifetime imaging to recognize different structures with similarfluorescence spectrum.Moreover,it can be facilely combined with multiphotonfluorescence microscopy.A single fs pulsed laser is utilized to achieve up-conversion(visible multiphotonfluorescence)and down-conversion(NIR-II one-photonfluorescence)excitation simultaneously,extending imaging spectral channels,and thus facilitates multi-structure and multi-functional observation.展开更多
The source area of the Yellow River(SAYR),located above the Huangheyan hydrological station,is important for ecological preservation and water source conservation in the Yellow River Basin.In this area,the impact of w...The source area of the Yellow River(SAYR),located above the Huangheyan hydrological station,is important for ecological preservation and water source conservation in the Yellow River Basin.In this area,the impact of water conservation projects on the hydrology and the ecological environment is pivotal in protecting water resources and alpine vegetation ecosystems.This study investigates the impact of the Yellow River Source Hydropower Station on the runoff and ecological evolution of the SAYR,along with the underlying mechanism,using extensive datasets encompassing long-term meteorological,hydrological and remote sensing data from various time periods.Results show that,over the long term,precipitation is the primary factor driving runoff variations in the SAYR.Nevertheless,from 1990 to 2020,there is a notably inconsistent relationship between precipitation and runoff.After the completion of the Yellow River Source Hydropower Station in 2001,the water level of Eling Lake experienced and elevation of 2–3 m,leading to a gradual recovery of runoff.In addition,the basin's water balance shifted from a negative to a positive equilibrium,oscillating with changes in lake water levels.Consequently,the overflow zone of the Tangchama alluvial–proluvial fan in the upper reaches of the lakeshore shifted by 500 m,and marsh wetlands expanded by 20.78 km^(2).The increased storage of lakes and groundwater in the SAYR is the key controlling factor for the runoff recovery,changes in the basin's water balance,and enhancements in lakeshore vegetation ecology.Under the geological background of the Qinghai–Tibet Plateau's upliftment and intensified upstream river erosion,the basin experienced a substantial water imbalance due to declining discharge base levels,which is the most critical factor behind runoff attenuation in the SAYR towards the end of the 20th century.The construction of the hydropower station objectively raised the drainage base level of the basin,thereby positively contributing to the preservation of water balance,runoff stability,and the enhancement of swamps and wetlands along the lakeshore.展开更多
BACKGROUND Although the location of proximal cancer of the remnant stomach is the same as that of primary proximal cancer of the stomach,its clinical characteristics and prognosis are still controversial.AIM To evalua...BACKGROUND Although the location of proximal cancer of the remnant stomach is the same as that of primary proximal cancer of the stomach,its clinical characteristics and prognosis are still controversial.AIM To evaluate the clinicopathological features and prognosis factors of gastric stump cancer(GSC)and primary proximal gastric cancer(PGC).METHODS From January,2005 to December,2016,178 patients with GSC and 957 cases with PGC who received surgical treatment were enrolled.Patients in both groups underwent 1:1 propensity score matching analysis,and both clinical and pathological data were systematically collected for statistical purposes.Quality of RESULTS One hundred and fifty-two pairs were successfully matched after propensity score matching analysis.Of the 15 demographic and pathological variables collected,the analysis further revealed that the number of lymph nodes and positive lymph nodes were different prognostic and clinicopathological factors between PGC and GSC.Univariate and multivariate analyses showed that gender,differentiation degree and tumor-node-metastasis stage were independent risk factors for patients with GSC.Gender,vascular invasion,differentiation degree,depth of infiltration,positive lymph nodes,and tumor-node-metastasis stage were independent risk factors for patients with PGC.The 5-year overall survival and cancer-specific survival of patients with GSC were significantly lower than those in the PGC group,the scores for overall quality of life in the GSC-malignant group were lower than the GSC-benign,and the differences were statistically significant.CONCLUSION The differences in clinicopathological characteristics between GSC and PGC were clarified,and PGC had a better prognosis than GSC.展开更多
BACKGROUND Whether patients over 85 years old with gastrointestinal cancer should undergo surgery remains controversial.We aimed to describe the changing trends of characteristics to provide more information to decisi...BACKGROUND Whether patients over 85 years old with gastrointestinal cancer should undergo surgery remains controversial.We aimed to describe the changing trends of characteristics to provide more information to decision makers,and strive to find appropriate surgical plan.AIM To describe the changing trends of characteristics to provide more information to decision makers,and strive to find appropriate surgical plan.METHODS A total of 218 gastric cancer(GC)patients and 563 colorectal cancer(CRC)patients who underwent surgery between 2001 and 2021 were enrolled in this retrospective analysis.Changes in clinicopathological features,surgical treatments,and survival status were analyzed longitudinally at 5-year intervals.RESULTS Only 14 GC patients underwent laparoscopic surgery where 219 CRC patients had this procedure.Cardia and esophagogastric junction cancer increased in GC patients,and the proportion of sigmoid colon cancer decreased in CRC patients.Pulmonary infection gradually became the most common postoperative complication,its incidence in period 4 reached 48.79%.However,the incidence of anastomotic leakage decreased from 26.79%to 9.38%(P<0.01).Additionally,30-d mortality significantly decreased from 32.14%to 9.01%.Increases were observed in 5-year overall survival(OS)in GC patients from period 1 to period 4(18.18%vs 33.32%,respectively)and CRC patients(0 vs 36.32%,respectively).Disease-free survival(DFS)also increased in GC and CRC patients(7.14%vs 27.74%and 0 to 36.03%,respectively).The average survival time of GC patients following radial lymphadenectomy was higher than in patients that underwent limited lymphadenectomy(26 vs 22 mo,respectively),the same was seen in CRC patients(44 vs 33 mo,respectively).This advantage was particularly evident in patients with TNM I,but not in patients with TNM II/III period cancer.CONCLUSION The safety as well as effectiveness of surgery in ultra-elderly patients is increasing.Radical lymphadenectomy has advantages in patients with TNM I gastrointestinal cancer,but not TNM II/III.展开更多
基金supported by National Natural Science Foundation of China(61975172,82001874 and 61735016).
文摘Fluorescence imaging in the second near-infrared window(NIR-II,900–1880 nm)with less scattering background in biological tissues has been combined with the confocal microscopic system for achieving deep in vivo imaging with high spatial resolution.However,the traditional NIR-IIfluorescence confocal microscope with separate excitation focus and detection pinhole makes it possess low confocal e±ciency,as well as di±cultly to adjust.Two types of upgraded NIR-IIfluorescence confocal microscopes,sharing the same pinhole by excitation and emission focus,leading to higher confocal e±ciency,are built in this work.One type is-ber-pinhole-based confocal microscope applicable to CW laser excitation.It is constructed forfluorescence intensity imaging with large depth,high stabilization and low cost,which could replace multiphotonfluorescence microscopy in some applications(e.g.,cerebrovascular and hepatocellular imaging).The other type is air-pinhole-based confocal microscope applicable to femtosecond(fs)laser excitation.It can be employed not only for NIR-IIfluorescence intensity imaging,but also for multi-channelfluorescence lifetime imaging to recognize different structures with similarfluorescence spectrum.Moreover,it can be facilely combined with multiphotonfluorescence microscopy.A single fs pulsed laser is utilized to achieve up-conversion(visible multiphotonfluorescence)and down-conversion(NIR-II one-photonfluorescence)excitation simultaneously,extending imaging spectral channels,and thus facilitates multi-structure and multi-functional observation.
基金funded by China Geological Survey Program(DD20230422)and Chinese Academy of Geological Sciences Basic Research Fund Program(SK202214).
文摘The source area of the Yellow River(SAYR),located above the Huangheyan hydrological station,is important for ecological preservation and water source conservation in the Yellow River Basin.In this area,the impact of water conservation projects on the hydrology and the ecological environment is pivotal in protecting water resources and alpine vegetation ecosystems.This study investigates the impact of the Yellow River Source Hydropower Station on the runoff and ecological evolution of the SAYR,along with the underlying mechanism,using extensive datasets encompassing long-term meteorological,hydrological and remote sensing data from various time periods.Results show that,over the long term,precipitation is the primary factor driving runoff variations in the SAYR.Nevertheless,from 1990 to 2020,there is a notably inconsistent relationship between precipitation and runoff.After the completion of the Yellow River Source Hydropower Station in 2001,the water level of Eling Lake experienced and elevation of 2–3 m,leading to a gradual recovery of runoff.In addition,the basin's water balance shifted from a negative to a positive equilibrium,oscillating with changes in lake water levels.Consequently,the overflow zone of the Tangchama alluvial–proluvial fan in the upper reaches of the lakeshore shifted by 500 m,and marsh wetlands expanded by 20.78 km^(2).The increased storage of lakes and groundwater in the SAYR is the key controlling factor for the runoff recovery,changes in the basin's water balance,and enhancements in lakeshore vegetation ecology.Under the geological background of the Qinghai–Tibet Plateau's upliftment and intensified upstream river erosion,the basin experienced a substantial water imbalance due to declining discharge base levels,which is the most critical factor behind runoff attenuation in the SAYR towards the end of the 20th century.The construction of the hydropower station objectively raised the drainage base level of the basin,thereby positively contributing to the preservation of water balance,runoff stability,and the enhancement of swamps and wetlands along the lakeshore.
文摘BACKGROUND Although the location of proximal cancer of the remnant stomach is the same as that of primary proximal cancer of the stomach,its clinical characteristics and prognosis are still controversial.AIM To evaluate the clinicopathological features and prognosis factors of gastric stump cancer(GSC)and primary proximal gastric cancer(PGC).METHODS From January,2005 to December,2016,178 patients with GSC and 957 cases with PGC who received surgical treatment were enrolled.Patients in both groups underwent 1:1 propensity score matching analysis,and both clinical and pathological data were systematically collected for statistical purposes.Quality of RESULTS One hundred and fifty-two pairs were successfully matched after propensity score matching analysis.Of the 15 demographic and pathological variables collected,the analysis further revealed that the number of lymph nodes and positive lymph nodes were different prognostic and clinicopathological factors between PGC and GSC.Univariate and multivariate analyses showed that gender,differentiation degree and tumor-node-metastasis stage were independent risk factors for patients with GSC.Gender,vascular invasion,differentiation degree,depth of infiltration,positive lymph nodes,and tumor-node-metastasis stage were independent risk factors for patients with PGC.The 5-year overall survival and cancer-specific survival of patients with GSC were significantly lower than those in the PGC group,the scores for overall quality of life in the GSC-malignant group were lower than the GSC-benign,and the differences were statistically significant.CONCLUSION The differences in clinicopathological characteristics between GSC and PGC were clarified,and PGC had a better prognosis than GSC.
基金The study was reviewed and approved by the Clinical Medical Research Ethics Committee of the First Affiliated Hospital of Anhui Medical University(PJ2023-07-56).
文摘BACKGROUND Whether patients over 85 years old with gastrointestinal cancer should undergo surgery remains controversial.We aimed to describe the changing trends of characteristics to provide more information to decision makers,and strive to find appropriate surgical plan.AIM To describe the changing trends of characteristics to provide more information to decision makers,and strive to find appropriate surgical plan.METHODS A total of 218 gastric cancer(GC)patients and 563 colorectal cancer(CRC)patients who underwent surgery between 2001 and 2021 were enrolled in this retrospective analysis.Changes in clinicopathological features,surgical treatments,and survival status were analyzed longitudinally at 5-year intervals.RESULTS Only 14 GC patients underwent laparoscopic surgery where 219 CRC patients had this procedure.Cardia and esophagogastric junction cancer increased in GC patients,and the proportion of sigmoid colon cancer decreased in CRC patients.Pulmonary infection gradually became the most common postoperative complication,its incidence in period 4 reached 48.79%.However,the incidence of anastomotic leakage decreased from 26.79%to 9.38%(P<0.01).Additionally,30-d mortality significantly decreased from 32.14%to 9.01%.Increases were observed in 5-year overall survival(OS)in GC patients from period 1 to period 4(18.18%vs 33.32%,respectively)and CRC patients(0 vs 36.32%,respectively).Disease-free survival(DFS)also increased in GC and CRC patients(7.14%vs 27.74%and 0 to 36.03%,respectively).The average survival time of GC patients following radial lymphadenectomy was higher than in patients that underwent limited lymphadenectomy(26 vs 22 mo,respectively),the same was seen in CRC patients(44 vs 33 mo,respectively).This advantage was particularly evident in patients with TNM I,but not in patients with TNM II/III period cancer.CONCLUSION The safety as well as effectiveness of surgery in ultra-elderly patients is increasing.Radical lymphadenectomy has advantages in patients with TNM I gastrointestinal cancer,but not TNM II/III.
