Twenty-nine facultative anaerobic cellulose-degrading bacteria were isolated from soil, rumen fluid, rumen residues and diet of dairy cow. Based on 16 Sr DNA analysis by BLAST algorithm method, the results showed that...Twenty-nine facultative anaerobic cellulose-degrading bacteria were isolated from soil, rumen fluid, rumen residues and diet of dairy cow. Based on 16 Sr DNA analysis by BLAST algorithm method, the results showed that most of the strains were Bacillus genera, and six of the 29 strains were bigger than 10 mm of diameter of clear zones. For them, two strains were isolated from rumen fluid(L5 and L7) and other two were isolated from rumen residue(N5 and N9), while others were isolated from soil(T1) and diet(S6), respectively. Strains from rumen fluid and residue had higher activities of FPCase and CMCase, but lower β-glucosidases.展开更多
The object of this study was to investigate the effects of chromium propionate replacing 25%rumen-protected choline(RPC)on production performance and blood indicators of perinatal dairy cows.According to the principle...The object of this study was to investigate the effects of chromium propionate replacing 25%rumen-protected choline(RPC)on production performance and blood indicators of perinatal dairy cows.According to the principle of ensuring that chromium propionate and RPC were fed 14 days prepartum,27 healthy Holstein cows(age,parity,lactation volume,body condition and expectancy were similar)were randomly divided into three groups(GroupsⅠ,ⅡandⅢ),with nine cows in each group.Cows in GroupⅠwere fed basal diets;cows in GroupⅡwere fed the same basal diets with 10 g•d-1 RPC per cow;and cows in GroupⅢwere fed the same basal diets,but 7.5 g RPC and 2.5 g chromium propionate(4 mg chromium)per cow.The results showed that dry matter intake(DMI)of prepartum in GroupsⅡandⅢwas significantly increased(P<0.05),compared with GroupⅠ;however,there was no significant difference between GroupsⅡandⅢ(P>0.05).The milk fat content in GroupⅢwas significantly higher than that in GroupⅠon the 7th day of postpartum(P<0.05).The urea nitrogen content in GroupsⅡandⅢsignificantly lower than that in GroupⅠon the 21st day of postpartum(P<0.05),but there were no significant difference between GroupsⅡandⅢ(P>0.05).The somatic cell counts in GroupⅢwere significantly lower than those in GroupsⅠandⅡon the 14th and 21st days of postpartum(P<0.05).The contents of the total cholesterol in GroupⅢdecreased significantly compared to GroupⅡon the 7th day of prepartum,on the day of calving and the 7th day of postpartum(P<0.05).The concentration of high-density lipoprotein(HDL)in GroupⅢwas significantly higher than that in GroupⅡon the 7th day of prepartum,on the day of calving and the 7th day of postpartum(P<0.05).On the 7th day of postpartum the concentration of insulin in GroupⅢwas obviously higher than that in GroupⅡ(P<0.05).In summary,replacing 25%of rumen-protected choline with chromium propionate reduced production costs and improved dairy farming benefits.展开更多
基金Supported by Northeast Agricultural University Innovation Funding for Postgraduate(yjscx14007)China Agriculture Research System(CARS-37)
文摘Twenty-nine facultative anaerobic cellulose-degrading bacteria were isolated from soil, rumen fluid, rumen residues and diet of dairy cow. Based on 16 Sr DNA analysis by BLAST algorithm method, the results showed that most of the strains were Bacillus genera, and six of the 29 strains were bigger than 10 mm of diameter of clear zones. For them, two strains were isolated from rumen fluid(L5 and L7) and other two were isolated from rumen residue(N5 and N9), while others were isolated from soil(T1) and diet(S6), respectively. Strains from rumen fluid and residue had higher activities of FPCase and CMCase, but lower β-glucosidases.
基金Supported by China Agriculture Research System(CARS-36)。
文摘The object of this study was to investigate the effects of chromium propionate replacing 25%rumen-protected choline(RPC)on production performance and blood indicators of perinatal dairy cows.According to the principle of ensuring that chromium propionate and RPC were fed 14 days prepartum,27 healthy Holstein cows(age,parity,lactation volume,body condition and expectancy were similar)were randomly divided into three groups(GroupsⅠ,ⅡandⅢ),with nine cows in each group.Cows in GroupⅠwere fed basal diets;cows in GroupⅡwere fed the same basal diets with 10 g•d-1 RPC per cow;and cows in GroupⅢwere fed the same basal diets,but 7.5 g RPC and 2.5 g chromium propionate(4 mg chromium)per cow.The results showed that dry matter intake(DMI)of prepartum in GroupsⅡandⅢwas significantly increased(P<0.05),compared with GroupⅠ;however,there was no significant difference between GroupsⅡandⅢ(P>0.05).The milk fat content in GroupⅢwas significantly higher than that in GroupⅠon the 7th day of postpartum(P<0.05).The urea nitrogen content in GroupsⅡandⅢsignificantly lower than that in GroupⅠon the 21st day of postpartum(P<0.05),but there were no significant difference between GroupsⅡandⅢ(P>0.05).The somatic cell counts in GroupⅢwere significantly lower than those in GroupsⅠandⅡon the 14th and 21st days of postpartum(P<0.05).The contents of the total cholesterol in GroupⅢdecreased significantly compared to GroupⅡon the 7th day of prepartum,on the day of calving and the 7th day of postpartum(P<0.05).The concentration of high-density lipoprotein(HDL)in GroupⅢwas significantly higher than that in GroupⅡon the 7th day of prepartum,on the day of calving and the 7th day of postpartum(P<0.05).On the 7th day of postpartum the concentration of insulin in GroupⅢwas obviously higher than that in GroupⅡ(P<0.05).In summary,replacing 25%of rumen-protected choline with chromium propionate reduced production costs and improved dairy farming benefits.
