In addition to the temperature and pressure conditions,the pore fluid composition and migration behavior are also crucial to control hydrate decomposition in the exploitation process.In this work,to investigate the ef...In addition to the temperature and pressure conditions,the pore fluid composition and migration behavior are also crucial to control hydrate decomposition in the exploitation process.In this work,to investigate the effects of these factors,a series of depressurization experiments were carried out in a visible one-dimensional reactor,using hydrate reservoir samples with water saturations ranging from 20%to 65%.The results showed a linear relationship between gas production rates and gas saturations of the reservoir,suggesting that a larger gas-phase space was conducive to hydrate decomposition and gas outflow.Therefore,the rapid water production in the early stage of hydrate exploitation could release more gas-phase space in the water-rich reservoir,which in turn improved the gas production efficiency.Meanwhile,the spatiotemporal evolution of pore fluids could lead to partial accelerated decomposition or secondary formation of hydrates.In the unsealed reservoir,the peripheral water infiltration kept reservoir at a high water saturation,which hindered the overall production process and caused higher water production.Importantly,depressurization assisted with the N2 sweep could displace the pore water rapidly.According to the results,it is recommended that using the short-term N2 sweep as an auxiliary means in the early stage of depressurization to expand the gas-phase space in order to achieve the highest production efficiency.展开更多
Transcription factors(TFs)control an array of expressed genes.However,the specifics of how a gene is expressed in time and space as controlled by a TF remain largely unknown.Here,in TRPC6-regulated proline oxidase 1(P...Transcription factors(TFs)control an array of expressed genes.However,the specifics of how a gene is expressed in time and space as controlled by a TF remain largely unknown.Here,in TRPC6-regulated proline oxidase 1(POX)transcription in human glioma,we report that OIP5-AS1,a long noncoding RNA,determines the specificity of p53-driven POX expression.The OIP5-AS1/p53 complex via its 24 nucleotides binds to the POX promoter and is necessary for POX expression but not for p21 transcription.An O-site in the POX promoter to which OIP5-AS1 binds was identified that is required for OIP5-AS1/p53 binding and POX transcription.Blocking OIP5-AS1 binding to the O-site inhibits POX transcription and promotes glioma development.Thus,the OIP5-AS1/O-site module decides p53-controlled POX expression as regulated by TRPC6 and affects glioma development.展开更多
基金financially supported by the National Natural Science Foundation of China,China(Nos.52004136,22127812,U20B6005)China Postdoctoral Science Foundation,China(Nos.2020M670347,2021T140382)Guangdong MEPP Fund(No.GDNRC[2021]055).
文摘In addition to the temperature and pressure conditions,the pore fluid composition and migration behavior are also crucial to control hydrate decomposition in the exploitation process.In this work,to investigate the effects of these factors,a series of depressurization experiments were carried out in a visible one-dimensional reactor,using hydrate reservoir samples with water saturations ranging from 20%to 65%.The results showed a linear relationship between gas production rates and gas saturations of the reservoir,suggesting that a larger gas-phase space was conducive to hydrate decomposition and gas outflow.Therefore,the rapid water production in the early stage of hydrate exploitation could release more gas-phase space in the water-rich reservoir,which in turn improved the gas production efficiency.Meanwhile,the spatiotemporal evolution of pore fluids could lead to partial accelerated decomposition or secondary formation of hydrates.In the unsealed reservoir,the peripheral water infiltration kept reservoir at a high water saturation,which hindered the overall production process and caused higher water production.Importantly,depressurization assisted with the N2 sweep could displace the pore water rapidly.According to the results,it is recommended that using the short-term N2 sweep as an auxiliary means in the early stage of depressurization to expand the gas-phase space in order to achieve the highest production efficiency.
基金This work was partially supported by the grant(81830043)from the National Natural Science Foundation of China(NSFC).
文摘Transcription factors(TFs)control an array of expressed genes.However,the specifics of how a gene is expressed in time and space as controlled by a TF remain largely unknown.Here,in TRPC6-regulated proline oxidase 1(POX)transcription in human glioma,we report that OIP5-AS1,a long noncoding RNA,determines the specificity of p53-driven POX expression.The OIP5-AS1/p53 complex via its 24 nucleotides binds to the POX promoter and is necessary for POX expression but not for p21 transcription.An O-site in the POX promoter to which OIP5-AS1 binds was identified that is required for OIP5-AS1/p53 binding and POX transcription.Blocking OIP5-AS1 binding to the O-site inhibits POX transcription and promotes glioma development.Thus,the OIP5-AS1/O-site module decides p53-controlled POX expression as regulated by TRPC6 and affects glioma development.
