1.Introduction b-Galactosidase(commonly known as b-lactase;EC 3.2.1.23)is a multifunctional enzyme that can catalyze the hydrolysis of terminal non-reducing b-D-galactose residues in b-D-galactosides or transfer the g...1.Introduction b-Galactosidase(commonly known as b-lactase;EC 3.2.1.23)is a multifunctional enzyme that can catalyze the hydrolysis of terminal non-reducing b-D-galactose residues in b-D-galactosides or transfer the galactosyl residue to saccharide acceptors to yield galactooligosaccharides(GOS).b-Galactosidase has a variety of applications in food and medical industries such as hydrolysis of lactose in milk,manufacture of galactooligosaccharides(GOS)and treatment of lactose malabsorption[1].Although b-galactosidase is an ubiquitous enzyme existing in plants,animals and microorganisms,only a few b-galactosidases from Kluveromyces lactis,Aspergillus niger and Aspergillus oryzae are regarded as safe for food related industry applications.展开更多
基金This work was supported by Key International Cooperation Project from Chinese Academy of Sciences(155112KYSB20160010)Beijing Municipal Natural Science Foundation(5132024)National Natural Science Foundation of China(31000026).
文摘1.Introduction b-Galactosidase(commonly known as b-lactase;EC 3.2.1.23)is a multifunctional enzyme that can catalyze the hydrolysis of terminal non-reducing b-D-galactose residues in b-D-galactosides or transfer the galactosyl residue to saccharide acceptors to yield galactooligosaccharides(GOS).b-Galactosidase has a variety of applications in food and medical industries such as hydrolysis of lactose in milk,manufacture of galactooligosaccharides(GOS)and treatment of lactose malabsorption[1].Although b-galactosidase is an ubiquitous enzyme existing in plants,animals and microorganisms,only a few b-galactosidases from Kluveromyces lactis,Aspergillus niger and Aspergillus oryzae are regarded as safe for food related industry applications.