Three-dimensional-printed collagen/chitosan/secretome derived from HUCMSCs scaffolds for efficient neural network reconstruction in canines with traumatic brain injury

The secretome secreted by stem cells and bioactive material has emerged as a promising therapeutic choice for traumatic brain injury(TBI).We aimed to determine the effect of 3D-printed collagen/chitosan/secretome derived from human umbilical cord blood mesenchymal stem cells scaffolds(3D-CC-ST)on the injured tissue regeneration process.3D-CC-ST was performed using 3D printing technology at a low temperature(20C),and the physical properties and degeneration rate were measured.The utilization of low temperature contributed to a higher cytocompatibility of fabricating porous 3D architectures that provide a homogeneous distribution of cells.Immediately after the establishment of the canine TBI model,3D-CC-ST and 3D-CC(3D-printed collagen/chitosan scaffolds)were implanted into the cavity of TBI.Following implantation of scaffolds,neurological examination and motor evoked potential detection were performed to analyze locomotor function recovery.Histological and immunofluorescence staining were performed to evaluate neuro-regeneration.The group treated with 3D-CC-ST had good performance of behavior functions.Implanting 3D-CC-ST significantly reduced the cavity area,facilitated the regeneration of nerve fibers and vessel reconstruction,and promoted endogenous neuronal differentiation and synapse formation after TBI.The implantation of 3D-CC-ST also markedly reduced cell apoptosis and regulated the level of systemic inflammatory factors after TBI.

Administration of B7-H3 targeted chimeric antigen receptor-T cells induce regression of glioblastoma

Dear Editor,Nowadays,glioblastoma(GBM)was the most common and lethal form of primary intracranial tumor.Despite standard-of-care therapy,GBM still exhibited a poor prognosis with 5-years survival rate less than 5%.Recent years,adoptive CAR-T therapy came to be a novel immunotherapy in treating malignant tumors.Great progress has been made by CD19 targeted CAR-T cells against refractory B cell cancers.Recent studies also reported about the clinical potential of CAR-T therapy targeting IL13Ra2 and EGFRvlll in treating GBM.1,2 However,limited numbers of therapeutic targets in GBM may preclude it from progress and being popularized.B7-H3(CD276)has been found to be overexpressed by many tumors and tumor-infiltrating dendritic cell.3 Our previous studies suggested the potent anti-tumor effect of B7-H3 targeted CAR-T cells against GBM in preclinical models.

Radiological biomarkers of idiopathic normal pressure hydrocephalus:new approaches for detecting concomitant Alzheimer’s disease and predicting prognosis

Idiopathic normal pressure hydrocephalus(iNPH)is a clinical syndrome characterized by cognitive decline,gait disturbance,and urinary incontinence.As iNPH often occurs in elderly individuals prone tomany types of comorbidity,a differential diagnosis with other neurodegenerative diseases is crucial,especially Alzheimer’s disease(AD).A growing body of published work provides evidence of radiological methods,including multimodal magnetic resonance imaging and positron emission tomography,which may help noninvasively differentiate iNPH from AD or reveal concurrent AD pathology in vivo.Imaging methods detecting morphological changes,white matter microstructural changes,cerebrospinal fluid circulation,and molecular imaging have been widely applied in iNPH patients.Here,we review radiological biomarkers using different methods in evaluating iNPH pathophysiology and differentiating or detecting concomitant AD,to noninvasively predict the possible outcome postshunt and select candidates for shunt surgery.