The pathogen Pseudomonas syringae,responsible for a variety of diseases,poses a considerable threat to global crop yields.Emerging biocontrol strategies employ antagonistic microorganisms,utilizing phyllosphere microe...The pathogen Pseudomonas syringae,responsible for a variety of diseases,poses a considerable threat to global crop yields.Emerging biocontrol strategies employ antagonistic microorganisms,utilizing phyllosphere microecology and systemic resistance to combat this disease.However,the interactions between phyllosphere microbial dynamics and the activation of the plant defense system remain poorly understood.Here we show significant alterations in phyllosphere microbiota structure and plant gene expression following the application of biocontrol agents.We reveal enhanced collaboration and integration of Sphingomonas and Methylobacterium within the microbial co-occurrence network.Notably,Sphingomonas inhibits P.syringae by disrupting pathogen chemotaxis and virulence.Additionally,both Sphingomonas and Methylobacterium activate plant defenses by upregulating pathogenesis-related gene expression through abscisic acid,ethylene,jasmonate acid,and salicylic acid signaling pathways.Our results highlighted that biocontrol agents promote plant health,from reconstructing beneficial microbial consortia to enhancing plant immunity.The findings enrich our comprehension of the synergistic interplays between phyllosphere microbiota and plant immunity,offering potential enhancements in biocontrol efficacy for crop protection.展开更多
The effective disposal of redundant tea waste is crucial to environmental protection and comprehensive utilization of trash resources. In this work, the removal of methyl orange （MO） from aqueous solution using spen...The effective disposal of redundant tea waste is crucial to environmental protection and comprehensive utilization of trash resources. In this work, the removal of methyl orange （MO） from aqueous solution using spent tea leaves as the sorbent was investigated in a batch experiment. First, the effects of various parameters such as temperature, adsorption time, dose of spent tea leaves, and initial concentration of MO were investigated. Then, the response surface methodology （RSM）, based on Box- Behnken design, was employed to obtain the optimum adsorption conditions. The optimal conditions could be obtained at an initial concentration of MO of 9.75 mg·L-1, temperature of 35.3℃, contact time of 63.8 min, and an adsorbent dosage 3.90 g· L-1. Under the optimized condi- tions, the maximal removal of MO was 58.2%. The results indicate that spent tea leaves could be used as an effective and economical adsorbent in the removal of MO from aqueous solution.展开更多
基金supported by the Key Project of Science and Technology Project of China National Tobacco Corporation(110202101027)[LS-11]the Key Research Project of Hunan Province(2023NK2019)+1 种基金the Natural Science Foundation of Changsha(kq2202089)the International Scientific and Technological Cooperation Base of Hunan(2018WK4019).
文摘The pathogen Pseudomonas syringae,responsible for a variety of diseases,poses a considerable threat to global crop yields.Emerging biocontrol strategies employ antagonistic microorganisms,utilizing phyllosphere microecology and systemic resistance to combat this disease.However,the interactions between phyllosphere microbial dynamics and the activation of the plant defense system remain poorly understood.Here we show significant alterations in phyllosphere microbiota structure and plant gene expression following the application of biocontrol agents.We reveal enhanced collaboration and integration of Sphingomonas and Methylobacterium within the microbial co-occurrence network.Notably,Sphingomonas inhibits P.syringae by disrupting pathogen chemotaxis and virulence.Additionally,both Sphingomonas and Methylobacterium activate plant defenses by upregulating pathogenesis-related gene expression through abscisic acid,ethylene,jasmonate acid,and salicylic acid signaling pathways.Our results highlighted that biocontrol agents promote plant health,from reconstructing beneficial microbial consortia to enhancing plant immunity.The findings enrich our comprehension of the synergistic interplays between phyllosphere microbiota and plant immunity,offering potential enhancements in biocontrol efficacy for crop protection.
文摘The effective disposal of redundant tea waste is crucial to environmental protection and comprehensive utilization of trash resources. In this work, the removal of methyl orange （MO） from aqueous solution using spent tea leaves as the sorbent was investigated in a batch experiment. First, the effects of various parameters such as temperature, adsorption time, dose of spent tea leaves, and initial concentration of MO were investigated. Then, the response surface methodology （RSM）, based on Box- Behnken design, was employed to obtain the optimum adsorption conditions. The optimal conditions could be obtained at an initial concentration of MO of 9.75 mg·L-1, temperature of 35.3℃, contact time of 63.8 min, and an adsorbent dosage 3.90 g· L-1. Under the optimized condi- tions, the maximal removal of MO was 58.2%. The results indicate that spent tea leaves could be used as an effective and economical adsorbent in the removal of MO from aqueous solution.
