A method was developed for preparing high purity Al30O8(OH)56(H2O)_(24)^(18)+(Al30) through elimination of impurities by complexation.Polyaluminum chloride II(PAC_(30))with Al_(c )content of 75%was adopted as the sour...A method was developed for preparing high purity Al30O8(OH)56(H2O)_(24)^(18)+(Al30) through elimination of impurities by complexation.Polyaluminum chloride II(PAC_(30))with Al_(c )content of 75%was adopted as the source of Al_(30).The PAC_(30)was prepared under conditions of total aluminum concentration 0.1 mol/L and OH^-/Al ratio 2.2 to obtain the highest content of Al_(30).A precipitation/metathesis method,organic solvent precipitation method and organic complexation method were examined to separate and purify Al_(30).It was found that only by the organic complexation method could high purity Al_(30)products be obtained in large yield economically.In the experiments,benzoic acid was used as the coordinating reagent to decompose the main impurity AlO_4Al_(12)(OH)_(24)(H_2O)_(12)^(7+)(Al_(13)),and the Al_(30)product could be obtained by precipitation and metathesis operations.It was noteworthy that the decomposition of impurities by benzoic acid could be completed in 2 hr.The Al_(30)product was characterized by Ferron assay,^(27)Al-NMR,SEM,XRD and TGA.The results showed that the purity of the Al_(30)product could exceed 92%.展开更多
Microbial synthesis utilizes sustainable resources to produce valuable chemicals,as a potential alternative to petroleum-based chemical industry.Although metabolic engineering is an efficient method to enhance the bio...Microbial synthesis utilizes sustainable resources to produce valuable chemicals,as a potential alternative to petroleum-based chemical industry.Although metabolic engineering is an efficient method to enhance the biosynthesis efficacy of microorganisms,it requires complicated biological procedures.Herein,we report a facile intracellular catalysis system for augmenting the production of bio-based material in microorganism.Covalent linking of oligo(p-phenylenevinylene)(OPV)and cyclopentadienyl rhodium(Ⅲ)bipyridine offers intracellular metal catalyst(OPV-Rh).The OPV-Rh complex displayed certain resistance to toxic biomolecules,which guaranteed its catalytic activity in complicated biological systems.With uptake by Gramnegative bacterium Ralstonia eutropha H16(R.eutropha H16),the OPV-Rh complex promotes the transformation of intracellular NADP+to NADPH,which further enhances the biosynthesis of polyhydroxybutyrate(PHB)by this microorganism.This work demonstrates that synthetic metal catalyst can be employed for regulating microbial biosynthesis intracellularly.展开更多
It is of great significance to develop effective antibacterial agents and methods to combat drug resistant bacterial infections due to its increasing threaten to human health and the ineffectiveness of antibiotics.Her...It is of great significance to develop effective antibacterial agents and methods to combat drug resistant bacterial infections due to its increasing threaten to human health and the ineffectiveness of antibiotics.Herein,a multifunctional hybrid nano-assembly(M1-Fe NPs)based on conjugated oligomer and ferrous ion was engineered with favorable bactericidal activity for synergetic antibacterial therapy.The chelation of ferrous ion not only enhances the photothermal conversion efficiency of M1 but also endows the nano-assembly with catalytic capability of transferring H_(2)O_(2) into stronger oxidant hydroxyl radicals(·OH).Meanwhile,the generated heat can further promote the Fenton reaction activity.By generating cytotoxic heat and oxidative·OH,M1-Fe NPs can effectively kill Staphylococcus aureus in vitro and in vivo with the aid of low dosage of H_(2)O_(2).The work provides a new multifunctional platform for combinational drug resistant antibacterial therapy and even antitumor therapy.展开更多
Nucleic acids are natural biopolymers of nucleotides that store, encode, transmit and express genetic information, which play central roles in diverse cellular events and diseases in living things. The analysis of nuc...Nucleic acids are natural biopolymers of nucleotides that store, encode, transmit and express genetic information, which play central roles in diverse cellular events and diseases in living things. The analysis of nucleic acids and nucleic acids-based analysis have been widely applied in biological studies, clinical diagnosis, environmental analysis, food safety and forensic analysis.During the past decades, the field of nucleic acids analysis has been rapidly advancing with many technological breakthroughs.In this review, we focus on the methods developed for analyzing nucleic acids, nucleic acids-based analysis, device for nucleic acids analysis, and applications of nucleic acids analysis. The representative strategies for the development of new nucleic acids analysis in this field are summarized, and key advantages and possible limitations are discussed. Finally, a brief perspective on existing challenges and further research development is provided.展开更多
Increasing multidrug-resistant(MDR) superbugs emerge worldwide causing a public health crisis. Consequently, it is urgent to find new antibiotics with efficient broad-spectrum antimicrobial activity. By virtue of vers...Increasing multidrug-resistant(MDR) superbugs emerge worldwide causing a public health crisis. Consequently, it is urgent to find new antibiotics with efficient broad-spectrum antimicrobial activity. By virtue of versatility in molecular design, a new peptide-like cell membrane-broken molecule, oligo-(7,7′-bifluoren-benzo[c][1,2,5]thiadiazole)(OFBT) possessing a conjugated backbone and eight pendant guanidyl moieties was designed and synthesized. OFBT exhibits favorable broad-spectrum of antimicrobial activity to pathogens including Gram-negative and Gram-positive bacteria, and fungi with the minimum inhibitory concentration(MIC)below 3.0 μM. Moreover, OFBT exhibits high selectivity for pathogens over human cells to make it a promising broad spectrum antimicrobial agent.展开更多
Conjugated polymers(CPs) are referred to a kind of fluorescent polymer materials with delocalized π-conjugated backbones.For the last decades, cationic CPs(CCPs) have been widely used in biosensor, imaging and biomed...Conjugated polymers(CPs) are referred to a kind of fluorescent polymer materials with delocalized π-conjugated backbones.For the last decades, cationic CPs(CCPs) have been widely used in biosensor, imaging and biomedical fields due to their good photophysical properties and solubility in water medium resulting from side chain modification with ionized moieties. In this mini-review, we mainly introduced the applications of CCPs in detection and inactivation of pathogen with typical examples, and also briefly discussed the relevant mechanisms. We hold the expectation that this mini-review can offer researchers a general reference and inspire them to construct new systems with high performances of pathogen detection and antimicrobial activity.展开更多
Conventional polymerizations mediated by living cells typically require synthetic transition-metal complexes or photoredox catalysts.Herein,we report an alternative photoinduced polymerization strategy for preparing f...Conventional polymerizations mediated by living cells typically require synthetic transition-metal complexes or photoredox catalysts.Herein,we report an alternative photoinduced polymerization strategy for preparing functional polymer hydrogels through bacteria-initiated radical polymerization of acrylamides in ordinary culture media.Upon light irradiation under ambient conditions,polyacrylamides were obtained with molecular weights of over 150 kDa using various bacteria.展开更多
Increasing intracellular drug concentration is an effec-tive way for cancer chemotherapeutics to enhance efficacy and combat drug resistance.In this work,a series of anticancer drug conjugates were prepared by linking...Increasing intracellular drug concentration is an effec-tive way for cancer chemotherapeutics to enhance efficacy and combat drug resistance.In this work,a series of anticancer drug conjugates were prepared by linking thiol-modified oligo(p-phenylene viny-lene)with paclitaxel,vincristine,teniposide,tamoxi-fen,doxorubicin,or podophyllotoxin(OPV-S-Drugs)through a Michael addition reaction.展开更多
Fluorescence switch plays a vital role in bioelectronics and bioimaging.Herein,we presented a new kind of facile electrostatic complex nanoparticles(ECNs)for fluorescence switching in cells and marking of individual c...Fluorescence switch plays a vital role in bioelectronics and bioimaging.Herein,we presented a new kind of facile electrostatic complex nanoparticles(ECNs)for fluorescence switching in cells and marking of individual cell.The ECNs were prepared by mixing positively charged poly(6-(2-(thiophen-3-yl)ethoxy)hexyl trimethylammonium bromide)(PT)and negatively charged diarylethene sodium salt(DAECOONa).DAE-COONa is a photoswitchable molecule which can be transformed between the ring-closed fo rm and ring-open form under the irradiation of UV or visible light.The closed-form of DAE-COONa can efficie ntly quench the fluorescence of PT through intermolecular energy transfer,while the open form of DAE-COONa does not influence the emission of PT.Thus,the fluorescence of ECNs can be modulated by light irradiation,and the ECNs with good fluorescence switching performance have been employed for fluorescence imaging and individual cell lighting up process successfully.We demonstrate that the electrostatic complex strategy provides a facile method to construct fluorescence switch fo r selective cell marking and imaging applications.展开更多
Three amphiphilic poly(fluorene-co-phenylene) derivatives with different side chains(PFP-1, PFP-2, PFP-3) were designed and synthesized for exploring their detection and imaging of pathogens. Upon incubation with six ...Three amphiphilic poly(fluorene-co-phenylene) derivatives with different side chains(PFP-1, PFP-2, PFP-3) were designed and synthesized for exploring their detection and imaging of pathogens. Upon incubation with six kinds of different pathogens, it was found the three polymers could selectively interact with Staphylococcus aureus(S. aureus). Their selective imaging towards S. aureus were thus realized. The selective imaging towards S. aureus was also confirmed even under the blend of microbes. PFP-3 shows stronger fluorescence imaging signal than PFP-1 and PFP-2. Zeta potential and isothermal titration microcalorimetry(ITC) tests demonstrated that both electrostatic interactions and hydrophobic interactions played important roles in the binding between PFPs and pathogens. Thus, amphiphilic PFP-3 exhibits great potential for specific imaging of S. aureus in a simple and rapid manner.展开更多
In this work,a high-performance fiber strain sensor is fabricated by constructing a double percolated structure,consisting of carbon nanotube(CNT)/thermoplastic polyurethane(TPU)continuous phase and styrene butadiene ...In this work,a high-performance fiber strain sensor is fabricated by constructing a double percolated structure,consisting of carbon nanotube(CNT)/thermoplastic polyurethane(TPU)continuous phase and styrene butadiene styrene(SBS)phase,incompatible with TPU(CNT/TPU@SBS).Compared with other similar fiber strain sensor systems without double percolated structure,the CNT/TPU@SBS sensor achieves a lower percolation threshold(0.38 wt.%)and higher electrical conductivity.The conductivity of 1%-CNT/TPU@SBS(4.12×10^(-3) S·m^(-1))is two orders of magnitude higher than that of 1%-CNT/TPU(3.17×10^(-5) S·m^(-1))at the same CNT loading of 1 wt.%.Due to double percolated structure,the 1%-CNT/TPU@SBS sensor exhibits a wide strain detection range(0.2%-100%)and an ultra-high sensitivity(maximum gauge factor(GF)is 32411 at 100%strain).Besides,the 1%-CNT/TPU@SBS sensor shows a high linearity(R^(2)=0.97)at 0%-20%strain,relatively fast response time(214 ms),and stability(500 loading/unloading cycles).The designed sensor can efficiently monitor physiological signals and movements and identify load distribution after being woven into a sensor array,showing broad application prospects in wearable electronics.展开更多
Optical imaging, as an important molecular imaging modality, has emerged many attractions in studying the biological or molecular events both in cell level and living subject because of its high resolution and sensiti...Optical imaging, as an important molecular imaging modality, has emerged many attractions in studying the biological or molecular events both in cell level and living subject because of its high resolution and sensitivity, noninvasive manner and low cost. Herein, we bring novel insights into a water-soluble conjugated polyelectlyte by deeply studying its properties in cells. Poly(9, 9-bis(6'-N,N,N-trimethylammonium hexyl)fluorene phenylene)(PFP), a good biosensing material, was studied in this paper. The biocompatibility of PFP was investigated in different cells, and cell cycle analysis was carried out to explore the reasons of different biocompatibility of PFP to cells. After irradiation, fluorescence enhancement of blue emission and turn-on of long-wavelength emission of PFP in HepG2 cells was observed, which was first reported as far as we know. The differentiated biocompatibility of PFP and its particular imaging properties in cancer cells can help to guide the application of conjugated polymers in cells and provide a new dimension in designing sensitive and responsive imaging materials.展开更多
To study the endocytic activity of dendritic cells(DCs)by obtaining fusion protein HSP70-EGFP as exogenous antigen and loading it with DCs derived from human peripheral blood.Fusion protein HSP70-EGFP was prokaryotica...To study the endocytic activity of dendritic cells(DCs)by obtaining fusion protein HSP70-EGFP as exogenous antigen and loading it with DCs derived from human peripheral blood.Fusion protein HSP70-EGFP was prokaryotically expressed,isolated and purified.DCs were isolated and cultured from human peripheral blood.The DCs were divided into 3 groups in the endocytic experiment.There were 106 DCs in each group.Group 1 and 2 were respectively incubated for 30 min.with HSP70-EGFP and EGFP.Group 3 was incubated with HSP70 for 30 min,and then incubated for 30 min.with HSP70-EGFP.Subsequently,3 groups were placed in an incubator at 37℃for 0.5,1,2 and 24 h.Flow cytometry(FCM)was adopted to detect the amount of DCs with EGFP inside.IL-12 Eli-spot was adopted to detect the amount of DCs which secreted IL-12.There were 5 types in the experiment:LPS,inactive LPS,HSP70-EGFP,EGFP and no antigen.Fusion protein HSP70-EGFP was successfully obtained and its molecular weight was 97000.It accounted for 35.32%of the total protein.Under irradiation of an ultraviolet lamp,the protein solution sent out viridescent fluorescence.The result detected by FCM indicated that after incubation for 0.5 h at 37℃,the positive rate in group 1 was 63%,while the other 2 groups were negative.After incubation for 1,2 and 24 h at 37℃,the positive rates in the 3 groups were above 80%.The IL-12 Eli-spot examination shows that with HSP70-EGFP being loaded,the amount of DCs secreting IL-12 was 134.09±31.78/10^(5)cells,a little lower than that of DCs with LPS loaded(with the average point of 156.36±15.73).There was no significant difference between the 2 groups(P<0.01).By contrast,both of them were significantly higher than inactive LPS-(33.78±1.40)/10^(5)cells and EGFP-loaded(23.13±4.57)/105 cells DC groups in the amount of DCs secreting IL-12(P<0.01).The results suggest that receptor-mediated phagocytosis plays a main role in the preliminary stage of DCs internalizing HSP70-EGFP.With increasing incubation time,pinocytosis begins to dominate.HSP70-EGFP may promote DCs to secret cell factor IL-12.展开更多
Infectious disease outbreaks have seriously endangered global health owing to the scarcity of testing materials and techniques.Diversified materials and methods should be urgently developed for rapid detection and dis...Infectious disease outbreaks have seriously endangered global health owing to the scarcity of testing materials and techniques.Diversified materials and methods should be urgently developed for rapid detection and discrimination of pathogenic microorganisms.Conjugated polymer(CP)materials are macromolecular compounds comprising numerous covalently bonded luminescent units.They have excellent light-harvesting and optical signal amplification capabilities owing to the transmission of excitation energy along their backbone.In recent years,CP materials have aroused research enthusiasm in the biosensors field because of their outstanding optoelectronic properties.This brief manuscript provides an overall review of recent progress achieved in CP-based systems for pathogen sensing.展开更多
The authors regret<the information of authors and affiliations“Libing Liu Qinxue Yang 2,3,Pin Wang 1,2,Feng Xiao 2,Ruyuan Jiao 2,Guangyu An 2,∗,Dongsheng Wang 2,∗1,2,1.University of Chinese Academy of Sciences,Bei...The authors regret<the information of authors and affiliations“Libing Liu Qinxue Yang 2,3,Pin Wang 1,2,Feng Xiao 2,Ruyuan Jiao 2,Guangyu An 2,∗,Dongsheng Wang 2,∗1,2,1.University of Chinese Academy of Sciences,Beijing 100085,China 2.State Key Laboratory of Environmental Aquatic Chemistry,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China3.Faculty Materials Science and Chemistry,China University of Geosciences,Wuhan 430074,China”should be changed to“Libing Liu 1,2,Qinxue Yang 1,3,Pin Wang 1,2,Feng Xiao 1,Ruyuan Jiao 1,Guangyu An 1,∗,Dongsheng Wang 1,∗1.State Key Laboratory of Environmental Aquatic Chemistry,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China 2.University of Chinese Academy of Sciences,Beijing 100049,China 3.Faculty Materials Science and Chemistry,China University of Geosciences,Wuhan 430074,China”>.展开更多
The widespread use of antibiotics causes the accumulation of a large amount of antibiotics in the environment.Excessively active antibiotics in the environment results in the emergence of bacterial resistance.Building...The widespread use of antibiotics causes the accumulation of a large amount of antibiotics in the environment.Excessively active antibiotics in the environment results in the emergence of bacterial resistance.Building smart antibiotics capable of reversible regulation between active and inactive states on demand is a promising approach to address this issue.Herein,a ferrocene-containing quaternary ammonium compound has been developed for electrochemical redox-controlled bacterial inhibition.The reversible switch of the reduced and oxidized ferrocene groups between hydrophobic and hydrophilic states triggers the assembly and disassembly of the micelles while modulating the interactions of antibiotic molecules with the bacteria membrane,providing a new way to regulate antibacterial activity.In addition,the alternate use of reduced and oxidized antibiotics exhibits a favorable effect in preventing bacterial resistance.Thus,an unconventional strategy is offered to prevent the build-up of active bactericide in the environment and decrease bacterial resistance.展开更多
基金supported by the National Natural Science Foundation of China (Nos. 21507149, 51338010)the National Water Pollution Control and Treatment Science and Technology Major Project (No. 2015ZX07406005-002)
文摘A method was developed for preparing high purity Al30O8(OH)56(H2O)_(24)^(18)+(Al30) through elimination of impurities by complexation.Polyaluminum chloride II(PAC_(30))with Al_(c )content of 75%was adopted as the source of Al_(30).The PAC_(30)was prepared under conditions of total aluminum concentration 0.1 mol/L and OH^-/Al ratio 2.2 to obtain the highest content of Al_(30).A precipitation/metathesis method,organic solvent precipitation method and organic complexation method were examined to separate and purify Al_(30).It was found that only by the organic complexation method could high purity Al_(30)products be obtained in large yield economically.In the experiments,benzoic acid was used as the coordinating reagent to decompose the main impurity AlO_4Al_(12)(OH)_(24)(H_2O)_(12)^(7+)(Al_(13)),and the Al_(30)product could be obtained by precipitation and metathesis operations.It was noteworthy that the decomposition of impurities by benzoic acid could be completed in 2 hr.The Al_(30)product was characterized by Ferron assay,^(27)Al-NMR,SEM,XRD and TGA.The results showed that the purity of the Al_(30)product could exceed 92%.
基金the National Natural Science Foundation of China(21533012,21661132006)。
文摘Microbial synthesis utilizes sustainable resources to produce valuable chemicals,as a potential alternative to petroleum-based chemical industry.Although metabolic engineering is an efficient method to enhance the biosynthesis efficacy of microorganisms,it requires complicated biological procedures.Herein,we report a facile intracellular catalysis system for augmenting the production of bio-based material in microorganism.Covalent linking of oligo(p-phenylenevinylene)(OPV)and cyclopentadienyl rhodium(Ⅲ)bipyridine offers intracellular metal catalyst(OPV-Rh).The OPV-Rh complex displayed certain resistance to toxic biomolecules,which guaranteed its catalytic activity in complicated biological systems.With uptake by Gramnegative bacterium Ralstonia eutropha H16(R.eutropha H16),the OPV-Rh complex promotes the transformation of intracellular NADP+to NADPH,which further enhances the biosynthesis of polyhydroxybutyrate(PHB)by this microorganism.This work demonstrates that synthetic metal catalyst can be employed for regulating microbial biosynthesis intracellularly.
基金the National Natural Science Foundation of China(Nos.21977065,22177065 and 21807067)Sanjin Scholars Support Planunder Special Funding(No.2017-06)+2 种基金Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi(Nos.201802106,2019L0022)Supported by the Fund for Shanxi“1331”Project(1331)the Program for Introducing Overseas High-level Talents of Shanxi(Hundred Talents Plan)。
文摘It is of great significance to develop effective antibacterial agents and methods to combat drug resistant bacterial infections due to its increasing threaten to human health and the ineffectiveness of antibiotics.Herein,a multifunctional hybrid nano-assembly(M1-Fe NPs)based on conjugated oligomer and ferrous ion was engineered with favorable bactericidal activity for synergetic antibacterial therapy.The chelation of ferrous ion not only enhances the photothermal conversion efficiency of M1 but also endows the nano-assembly with catalytic capability of transferring H_(2)O_(2) into stronger oxidant hydroxyl radicals(·OH).Meanwhile,the generated heat can further promote the Fenton reaction activity.By generating cytotoxic heat and oxidative·OH,M1-Fe NPs can effectively kill Staphylococcus aureus in vitro and in vivo with the aid of low dosage of H_(2)O_(2).The work provides a new multifunctional platform for combinational drug resistant antibacterial therapy and even antitumor therapy.
文摘Nucleic acids are natural biopolymers of nucleotides that store, encode, transmit and express genetic information, which play central roles in diverse cellular events and diseases in living things. The analysis of nucleic acids and nucleic acids-based analysis have been widely applied in biological studies, clinical diagnosis, environmental analysis, food safety and forensic analysis.During the past decades, the field of nucleic acids analysis has been rapidly advancing with many technological breakthroughs.In this review, we focus on the methods developed for analyzing nucleic acids, nucleic acids-based analysis, device for nucleic acids analysis, and applications of nucleic acids analysis. The representative strategies for the development of new nucleic acids analysis in this field are summarized, and key advantages and possible limitations are discussed. Finally, a brief perspective on existing challenges and further research development is provided.
基金supported by the National Natural Science Foundation of China (21533012, 21473220)Strategic Priority Research Program of the Chinese Academy of Sciences (XDA09030306)
文摘Increasing multidrug-resistant(MDR) superbugs emerge worldwide causing a public health crisis. Consequently, it is urgent to find new antibiotics with efficient broad-spectrum antimicrobial activity. By virtue of versatility in molecular design, a new peptide-like cell membrane-broken molecule, oligo-(7,7′-bifluoren-benzo[c][1,2,5]thiadiazole)(OFBT) possessing a conjugated backbone and eight pendant guanidyl moieties was designed and synthesized. OFBT exhibits favorable broad-spectrum of antimicrobial activity to pathogens including Gram-negative and Gram-positive bacteria, and fungi with the minimum inhibitory concentration(MIC)below 3.0 μM. Moreover, OFBT exhibits high selectivity for pathogens over human cells to make it a promising broad spectrum antimicrobial agent.
基金supported by the National Natural Science Foundation of China (21473221, 21473220)
文摘Conjugated polymers(CPs) are referred to a kind of fluorescent polymer materials with delocalized π-conjugated backbones.For the last decades, cationic CPs(CCPs) have been widely used in biosensor, imaging and biomedical fields due to their good photophysical properties and solubility in water medium resulting from side chain modification with ionized moieties. In this mini-review, we mainly introduced the applications of CCPs in detection and inactivation of pathogen with typical examples, and also briefly discussed the relevant mechanisms. We hold the expectation that this mini-review can offer researchers a general reference and inspire them to construct new systems with high performances of pathogen detection and antimicrobial activity.
基金supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(grant no.XDA16020804)the National Natural Science Foundation of China(grant nos.21871016,22021002,and 220201020050).
文摘Conventional polymerizations mediated by living cells typically require synthetic transition-metal complexes or photoredox catalysts.Herein,we report an alternative photoinduced polymerization strategy for preparing functional polymer hydrogels through bacteria-initiated radical polymerization of acrylamides in ordinary culture media.Upon light irradiation under ambient conditions,polyacrylamides were obtained with molecular weights of over 150 kDa using various bacteria.
基金The authors are grateful to the National Natural Sci-ence Foundation of China(Nos.21473221,91527306,and 21661132006)the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDA16020804)the Youth Innovation Promotion Association Chinese Academy of Sciences(No.2016029).
文摘Increasing intracellular drug concentration is an effec-tive way for cancer chemotherapeutics to enhance efficacy and combat drug resistance.In this work,a series of anticancer drug conjugates were prepared by linking thiol-modified oligo(p-phenylene viny-lene)with paclitaxel,vincristine,teniposide,tamoxi-fen,doxorubicin,or podophyllotoxin(OPV-S-Drugs)through a Michael addition reaction.
基金supported by the National Natural Science Foundation of China(Nos.91527306,21661132006)the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDA16020804)the Youth Innovation Promotion Association CAS(No.2016029)。
文摘Fluorescence switch plays a vital role in bioelectronics and bioimaging.Herein,we presented a new kind of facile electrostatic complex nanoparticles(ECNs)for fluorescence switching in cells and marking of individual cell.The ECNs were prepared by mixing positively charged poly(6-(2-(thiophen-3-yl)ethoxy)hexyl trimethylammonium bromide)(PT)and negatively charged diarylethene sodium salt(DAECOONa).DAE-COONa is a photoswitchable molecule which can be transformed between the ring-closed fo rm and ring-open form under the irradiation of UV or visible light.The closed-form of DAE-COONa can efficie ntly quench the fluorescence of PT through intermolecular energy transfer,while the open form of DAE-COONa does not influence the emission of PT.Thus,the fluorescence of ECNs can be modulated by light irradiation,and the ECNs with good fluorescence switching performance have been employed for fluorescence imaging and individual cell lighting up process successfully.We demonstrate that the electrostatic complex strategy provides a facile method to construct fluorescence switch fo r selective cell marking and imaging applications.
基金supported by the National Natural Science Foundation of China(21773268,21473220)the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA16020804)
文摘Three amphiphilic poly(fluorene-co-phenylene) derivatives with different side chains(PFP-1, PFP-2, PFP-3) were designed and synthesized for exploring their detection and imaging of pathogens. Upon incubation with six kinds of different pathogens, it was found the three polymers could selectively interact with Staphylococcus aureus(S. aureus). Their selective imaging towards S. aureus were thus realized. The selective imaging towards S. aureus was also confirmed even under the blend of microbes. PFP-3 shows stronger fluorescence imaging signal than PFP-1 and PFP-2. Zeta potential and isothermal titration microcalorimetry(ITC) tests demonstrated that both electrostatic interactions and hydrophobic interactions played important roles in the binding between PFPs and pathogens. Thus, amphiphilic PFP-3 exhibits great potential for specific imaging of S. aureus in a simple and rapid manner.
基金This work was supported by the National Natural Science Foundation of China(Grant No.12102374)the National Key Research and Development Program(Grant No.2019YFE0120300)+2 种基金the Sichuan Science and Technology Program(Grant No.2021YFH0031)the International Cooperation Project of Chengdu(Grant No.2019-GH02-00054-HZ)the Innovative Research Team of SWPU(Grant No.2017CXTD01).
文摘In this work,a high-performance fiber strain sensor is fabricated by constructing a double percolated structure,consisting of carbon nanotube(CNT)/thermoplastic polyurethane(TPU)continuous phase and styrene butadiene styrene(SBS)phase,incompatible with TPU(CNT/TPU@SBS).Compared with other similar fiber strain sensor systems without double percolated structure,the CNT/TPU@SBS sensor achieves a lower percolation threshold(0.38 wt.%)and higher electrical conductivity.The conductivity of 1%-CNT/TPU@SBS(4.12×10^(-3) S·m^(-1))is two orders of magnitude higher than that of 1%-CNT/TPU(3.17×10^(-5) S·m^(-1))at the same CNT loading of 1 wt.%.Due to double percolated structure,the 1%-CNT/TPU@SBS sensor exhibits a wide strain detection range(0.2%-100%)and an ultra-high sensitivity(maximum gauge factor(GF)is 32411 at 100%strain).Besides,the 1%-CNT/TPU@SBS sensor shows a high linearity(R^(2)=0.97)at 0%-20%strain,relatively fast response time(214 ms),and stability(500 loading/unloading cycles).The designed sensor can efficiently monitor physiological signals and movements and identify load distribution after being woven into a sensor array,showing broad application prospects in wearable electronics.
基金the National Natural Science Foundation of China (Nos. 21473220 and 51573002)the Major Research Plan of China (No. 2013CB932800) the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB12030300)
文摘Optical imaging, as an important molecular imaging modality, has emerged many attractions in studying the biological or molecular events both in cell level and living subject because of its high resolution and sensitivity, noninvasive manner and low cost. Herein, we bring novel insights into a water-soluble conjugated polyelectlyte by deeply studying its properties in cells. Poly(9, 9-bis(6'-N,N,N-trimethylammonium hexyl)fluorene phenylene)(PFP), a good biosensing material, was studied in this paper. The biocompatibility of PFP was investigated in different cells, and cell cycle analysis was carried out to explore the reasons of different biocompatibility of PFP to cells. After irradiation, fluorescence enhancement of blue emission and turn-on of long-wavelength emission of PFP in HepG2 cells was observed, which was first reported as far as we know. The differentiated biocompatibility of PFP and its particular imaging properties in cancer cells can help to guide the application of conjugated polymers in cells and provide a new dimension in designing sensitive and responsive imaging materials.
基金The study was supported by the General Program of National Natural Science Foundation of China(Grant No.30400167).
文摘To study the endocytic activity of dendritic cells(DCs)by obtaining fusion protein HSP70-EGFP as exogenous antigen and loading it with DCs derived from human peripheral blood.Fusion protein HSP70-EGFP was prokaryotically expressed,isolated and purified.DCs were isolated and cultured from human peripheral blood.The DCs were divided into 3 groups in the endocytic experiment.There were 106 DCs in each group.Group 1 and 2 were respectively incubated for 30 min.with HSP70-EGFP and EGFP.Group 3 was incubated with HSP70 for 30 min,and then incubated for 30 min.with HSP70-EGFP.Subsequently,3 groups were placed in an incubator at 37℃for 0.5,1,2 and 24 h.Flow cytometry(FCM)was adopted to detect the amount of DCs with EGFP inside.IL-12 Eli-spot was adopted to detect the amount of DCs which secreted IL-12.There were 5 types in the experiment:LPS,inactive LPS,HSP70-EGFP,EGFP and no antigen.Fusion protein HSP70-EGFP was successfully obtained and its molecular weight was 97000.It accounted for 35.32%of the total protein.Under irradiation of an ultraviolet lamp,the protein solution sent out viridescent fluorescence.The result detected by FCM indicated that after incubation for 0.5 h at 37℃,the positive rate in group 1 was 63%,while the other 2 groups were negative.After incubation for 1,2 and 24 h at 37℃,the positive rates in the 3 groups were above 80%.The IL-12 Eli-spot examination shows that with HSP70-EGFP being loaded,the amount of DCs secreting IL-12 was 134.09±31.78/10^(5)cells,a little lower than that of DCs with LPS loaded(with the average point of 156.36±15.73).There was no significant difference between the 2 groups(P<0.01).By contrast,both of them were significantly higher than inactive LPS-(33.78±1.40)/10^(5)cells and EGFP-loaded(23.13±4.57)/105 cells DC groups in the amount of DCs secreting IL-12(P<0.01).The results suggest that receptor-mediated phagocytosis plays a main role in the preliminary stage of DCs internalizing HSP70-EGFP.With increasing incubation time,pinocytosis begins to dominate.HSP70-EGFP may promote DCs to secret cell factor IL-12.
基金supported by the Natural Science Foundation of China(No.21704005)Fundamental Research Funds for the Cen-tral Universities(No.300102310106).
文摘Infectious disease outbreaks have seriously endangered global health owing to the scarcity of testing materials and techniques.Diversified materials and methods should be urgently developed for rapid detection and discrimination of pathogenic microorganisms.Conjugated polymer(CP)materials are macromolecular compounds comprising numerous covalently bonded luminescent units.They have excellent light-harvesting and optical signal amplification capabilities owing to the transmission of excitation energy along their backbone.In recent years,CP materials have aroused research enthusiasm in the biosensors field because of their outstanding optoelectronic properties.This brief manuscript provides an overall review of recent progress achieved in CP-based systems for pathogen sensing.
文摘The authors regret<the information of authors and affiliations“Libing Liu Qinxue Yang 2,3,Pin Wang 1,2,Feng Xiao 2,Ruyuan Jiao 2,Guangyu An 2,∗,Dongsheng Wang 2,∗1,2,1.University of Chinese Academy of Sciences,Beijing 100085,China 2.State Key Laboratory of Environmental Aquatic Chemistry,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China3.Faculty Materials Science and Chemistry,China University of Geosciences,Wuhan 430074,China”should be changed to“Libing Liu 1,2,Qinxue Yang 1,3,Pin Wang 1,2,Feng Xiao 1,Ruyuan Jiao 1,Guangyu An 1,∗,Dongsheng Wang 1,∗1.State Key Laboratory of Environmental Aquatic Chemistry,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China 2.University of Chinese Academy of Sciences,Beijing 100049,China 3.Faculty Materials Science and Chemistry,China University of Geosciences,Wuhan 430074,China”>.
基金supported by the National Natural Science Foundation of China(22021002,22020102005,and 22022705)the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA16020804)。
基金supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(no.XDA16020804)the National Natural Science Foundation of China(nos.91527306,21533012,21661132006,and 81503017).
文摘The widespread use of antibiotics causes the accumulation of a large amount of antibiotics in the environment.Excessively active antibiotics in the environment results in the emergence of bacterial resistance.Building smart antibiotics capable of reversible regulation between active and inactive states on demand is a promising approach to address this issue.Herein,a ferrocene-containing quaternary ammonium compound has been developed for electrochemical redox-controlled bacterial inhibition.The reversible switch of the reduced and oxidized ferrocene groups between hydrophobic and hydrophilic states triggers the assembly and disassembly of the micelles while modulating the interactions of antibiotic molecules with the bacteria membrane,providing a new way to regulate antibacterial activity.In addition,the alternate use of reduced and oxidized antibiotics exhibits a favorable effect in preventing bacterial resistance.Thus,an unconventional strategy is offered to prevent the build-up of active bactericide in the environment and decrease bacterial resistance.