Recording the highly diverse and dynamic activities in large populations of neurons in behaving animals is crucial for a better understanding of how the brain works.To meet this challenge,extensive efforts have been d...Recording the highly diverse and dynamic activities in large populations of neurons in behaving animals is crucial for a better understanding of how the brain works.To meet this challenge,extensive efforts have been devoted to developing functional fluorescent indicators and optical imaging techniques to optically monitor neural activity.Indeed,optical imaging potentially has extremely high throughput due to its non-invasive access to large brain regions and capability to sample neurons at high density,but the readout speed,such as the scanning speed in two-photon scanning microscopy,is often limited by various practical considerations.Among different imaging methods,light field microscopy features a highly parallelized 3D fluorescence imaging scheme and therefore promises a novel and faster strategy for functional imaging of neural activity.Here,we briefly review the working principles of various types of light field microscopes and their recent developments and applications in neuroscience studies.We also discuss strategies and considerations of optimizing light field microscopy for different experimental purposes,with illustrative examples in imaging zebrafish and mouse brains.展开更多
基金This work was supported by grants from the National Science and Technology Innovation 2030 Major Program(2021ZD0204503)National Key R&D Program of China(2017YFA0700504)+3 种基金the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB32030200)the International Partnership Program of the Chinese Academy of Sciences(153D31KYSB20170059)Shanghai Municipal Science and Technology Major Project(2018SHZDZX05)the National Natural Science Foundation of China(31871086 and 32125020).
文摘Recording the highly diverse and dynamic activities in large populations of neurons in behaving animals is crucial for a better understanding of how the brain works.To meet this challenge,extensive efforts have been devoted to developing functional fluorescent indicators and optical imaging techniques to optically monitor neural activity.Indeed,optical imaging potentially has extremely high throughput due to its non-invasive access to large brain regions and capability to sample neurons at high density,but the readout speed,such as the scanning speed in two-photon scanning microscopy,is often limited by various practical considerations.Among different imaging methods,light field microscopy features a highly parallelized 3D fluorescence imaging scheme and therefore promises a novel and faster strategy for functional imaging of neural activity.Here,we briefly review the working principles of various types of light field microscopes and their recent developments and applications in neuroscience studies.We also discuss strategies and considerations of optimizing light field microscopy for different experimental purposes,with illustrative examples in imaging zebrafish and mouse brains.
