Circulating lncRNA MHRT predicts survival of patients with chronic heart failure

Background It has been reported that lncRNA myosin heavy-chain-associated RNA transcripts(MHRT)can inhibit the apoptosis of cardiomyocytes.It is one of the major pathological changes leading to chronic heart failure.Methods The expression level of lncRNA MHRT was assessed by qRT-PCR.Diagnostic values of lncRNA MHRT for chronic heart failure were analyzed by ROC curve analysis.Cell apoptosis was detected by cell apoptosis assay.Results The results demonstrated that expression of lncRNA MHRT in plasma was down-regulated in patients with chronic heart failure compared to that in healthy people.Down-regulation of lncRNA MHRT distinguished chronic heart failure from healthy people.Over-expression of lncRNA MHRT inhibited the apoptosis of human cardiomyocyte cell line AC16 after H2O2 treatment.Follow-up study showed that chronic heart failure patients with lower expression levels of lncRNA MHRT had worse survival conditions compared to patients with higher expression levels of lncRNA MHRT.Conclusion We concluded that circulating lncRNA MHRT might serve as a diagnostic and prognostic marker for chronic heart failure treatment.

分泌型白细胞蛋白酶抑制因子对无创呼吸机治疗重症肺炎患者的预测效能

目的:分析下呼吸道分泌物分泌型白细胞蛋白酶抑制因子(SLPI)对无创呼吸机(NIV)治疗重症肺炎(SP)并发呼吸衰竭(RF)患者28 d生存的预测效能。方法:选取2021年4月至2023年4月绍兴市第七人民医院和绍兴市人民医院收治经NIV治疗的SP并发RF患者82例,根据28 d生存情况分为死亡组(25例)及生存组(57例)。比较两组患者的一般临床资料及下呼吸道分泌物SLPI水平。经logistic回归模型分析NIV治疗SP并发RF患者28 d生存的影响因素,经受试者工作特征(ROC)曲线分析各影响因素对NIV治疗SP并发RF患者28 d生存的预测效能。结果:与生存组比较,死亡组患者年龄较大[62(43,75)岁vs.64(52,78)岁,Z=3.044,P=0.003],重度RF占比[26.32%(15/57)vs.52.00%(13/25),χ^(2)=5.098,P=0.024]、致病菌种类≥2占比[35.09%(20/57)vs.60.00%(15/25),χ^(2)=4.408,P=0.036]及急性病生理学和长期健康评价(APACHE)Ⅱ评分[17(10,21)分vs.20(13,23)分,Z=3.140,P=0.002]均显著升高,氧合指数[(325±60)mmHg vs.(284±32)mmHg,t=3.284,P=0.002]及SLPI水平[(843±102)μg/L vs.(735±93)μg/L,t=4.524,P<0.001]均显著降低。Logistic回归模型分析显示,年龄[比值比(OR)=2.240,95%置信区间(CI)(1.340,3.745),P=0.002]、致病菌种类≥2[OR=2.804,95%CI(1.447,5.434),P=0.002]、APACHEⅡ评分[OR=2.598,95%CI(1.268,5.324),P=0.009]是行NIV治疗的SP并发RF患者28 d生存的危险因素,氧合指数[OR=0.582,95%CI(0.383,0.883),P=0.011]、SLPI[OR=0.507,95%CI(0.281,0.917),P=0.019]是其保护因素。ROC曲线显示,SLPI预测NIV治疗的SP并发RF患者28 d生存的最佳截断值为806.50μg/L,曲线下面积(AUC)为0.788[95%CI(0.684,0.871),P<0.001],且其AUC均高于年龄、致病菌种类≥2、APACHEⅡ评分、氧合指数(Z=1.989,P=0.047;Z=2.629,P=0.009;Z=2.231,P=0.026;Z=2.438,P=0.015)。结论:下呼吸道分泌物SLPI是行NIV治疗SP并发RF患者28 d生存的保护性因素,且预测其生存情况的效能良好。

Controllable synthesis of two-dimensional tungsten nitride nanosheets as electrocatalysts for oxygen reduction reaction

A facile synthetic strategy was developed for insitu preparation of two-dimensional (2D)highly crystalline tungsten nitride (WN)nanosheets with controllable morphology as oxygen reduction reaction (ORR)catalysts.The dependence of the crystal structure and morphology of WN on K2SO4content,pH,and pyrolysis temperature was thoroughly examined.The electrocatalytic performance of WN toward ORR in an alkaline electrolyte indicated that K+plays an important role in the control of size and shape in the hydrothermal and nitridation process,thereby promoting the formation of plate-like WO3and 2D WN nanosheets.The WN nanosheets,with largely exposed edge sites,provide abundant catalytic active sites and allow fast charge transfer.Furthermore,they exhibit high stability for ORR and methanol tolerance.