Photoelectron diffraction is an effective tool to probe the structures of molecules.The higher the photoelectron kinetic energy is,the higher order the diffraction pattern is disclosed in.Up to date,either the multi-a...Photoelectron diffraction is an effective tool to probe the structures of molecules.The higher the photoelectron kinetic energy is,the higher order the diffraction pattern is disclosed in.Up to date,either the multi-atomic molecule with the photoelectron kinetic energy below 150 e V or the diatomic molecule with 735 eV photoelectron has been experimentally reported.In this study,we measured the diffraction pattern of C 1 s and O 1 s photoelectrons in CO_(2)with 319.7 and 433.5 eV kinetic energies,respectively.The extracted C–O bond lengths are longer than the C–O bond length at the ground state,which is attributed to the asymmetric fragmentation that preferentially occurs at the longer chemical bond side during the zero-energy asymmetric vibration.展开更多
Objective and Impact Statement.Distinguishing malignant lymphocytes from normal ones is vital in pathological examination.We proposed an inverse light scattering(ILS)method for label-free suspended lymphocytes with co...Objective and Impact Statement.Distinguishing malignant lymphocytes from normal ones is vital in pathological examination.We proposed an inverse light scattering(ILS)method for label-free suspended lymphocytes with complex fine structures to identify their volumes for pathological state.Introduction.Light scattering as cell’s“fingerprint”provides valuable morphology information closely related to its biophysical states.However,the detail relationships between the morphology with complex fine structures and its scattering characters are not fully understood.Methods.To quantitatively inverse the volumes of membrane and nucleus as the main scatterers,clinical lymphocyte morphologies were modeled combining the Gaussian random sphere geometry algorithm by 750 reconstructed results after confocal scanning,which allowed the accurate simulation to solve ILS problem.For complex fine structures,the specificity for ILS study was firstly discussed(to our knowledge)considering the differences of not only surface roughness,posture,but also the ratio of nucleus to the cytoplasm and refractive index.Results.The volumes of membrane and nucleus were proved theoretically to have good linear relationship with the effective area and entropy of forward scattering images.Their specificity deviations were less than 3.5%.Then,our experimental results for microsphere and clinical leukocytes showed the Pearson product-moment correlation coefficients(PPMCC)of this linear relationship were up to 0.9830~0.9926.Conclusion.Our scattering inversion method could be effectively applied to identify suspended label-free lymphocytes without destructive sample pretreatments and complex experimental systems.展开更多
基金Supported by the National Natural Science Foundation of China(Grant No.11574020)The experiment was performed at the PLEIADES beamline at the SOLEIL Synchrotron,France(Grant No.20130821)。
文摘Photoelectron diffraction is an effective tool to probe the structures of molecules.The higher the photoelectron kinetic energy is,the higher order the diffraction pattern is disclosed in.Up to date,either the multi-atomic molecule with the photoelectron kinetic energy below 150 e V or the diatomic molecule with 735 eV photoelectron has been experimentally reported.In this study,we measured the diffraction pattern of C 1 s and O 1 s photoelectrons in CO_(2)with 319.7 and 433.5 eV kinetic energies,respectively.The extracted C–O bond lengths are longer than the C–O bond length at the ground state,which is attributed to the asymmetric fragmentation that preferentially occurs at the longer chemical bond side during the zero-energy asymmetric vibration.
基金supported by the National Natural Science Foundation of China (Grant Number:61875160).
文摘Objective and Impact Statement.Distinguishing malignant lymphocytes from normal ones is vital in pathological examination.We proposed an inverse light scattering(ILS)method for label-free suspended lymphocytes with complex fine structures to identify their volumes for pathological state.Introduction.Light scattering as cell’s“fingerprint”provides valuable morphology information closely related to its biophysical states.However,the detail relationships between the morphology with complex fine structures and its scattering characters are not fully understood.Methods.To quantitatively inverse the volumes of membrane and nucleus as the main scatterers,clinical lymphocyte morphologies were modeled combining the Gaussian random sphere geometry algorithm by 750 reconstructed results after confocal scanning,which allowed the accurate simulation to solve ILS problem.For complex fine structures,the specificity for ILS study was firstly discussed(to our knowledge)considering the differences of not only surface roughness,posture,but also the ratio of nucleus to the cytoplasm and refractive index.Results.The volumes of membrane and nucleus were proved theoretically to have good linear relationship with the effective area and entropy of forward scattering images.Their specificity deviations were less than 3.5%.Then,our experimental results for microsphere and clinical leukocytes showed the Pearson product-moment correlation coefficients(PPMCC)of this linear relationship were up to 0.9830~0.9926.Conclusion.Our scattering inversion method could be effectively applied to identify suspended label-free lymphocytes without destructive sample pretreatments and complex experimental systems.
