Optical diagnosis of dermatofibrosarcoma protuberans differentiated from dermatofibroma using non-linear optical microscopy

To the Editor:Dermatofibrosarcoma protuberans(DFSP)is a locally aggressive mesenchymal neoplasm of the skin that can be misdiagnosed as dermatofibroma(DF)because of similar histological characteristics.[1,2,3]In this study,non-linear optical(NLO)microscopy was used for imaging unstained histological sections to make a differential diagnosis between DFSP and DF.This study aimed to demonstrate that label-free NLO imaging provides significant advantages over standard hematoxylin-eosin(H&E)staining and allows pathologists to increase the diagnostic accuracy by using a quantifiable methodology.

基于多光子显微成像的隆突性皮肤纤维肉瘤定量分析

多光子显微成像是一种新型的无创诊断技术。与传统的诊断方法相比,多光子显微镜具有穿透深度更深且光毒性和光漂白较低等优势,在皮肤病学领域具有广阔的应用前景。本研究的目的是通过利用多光子显微成像技术对隆突性皮肤纤维肉瘤的未染色组织切片进行成像,试图寻找一种有效的无损成像方法,替代传统的基于苏木精和伊红(H&E)染色的病理诊断,同时提高DFSP诊断的准确性。结果表明,利用多光子显微镜对无H&E染色的组织切片进行成像,可以识别DFSP表皮和真皮中的细胞和基质以及皮下组织的组织学形态,结果与传统组织学分析表征类似。