miRNA-148b-3p targeting SOCS3 inhibits macrophage M2 polarization by JAK2/STAT3 pathway in immune thrombocytopenia

Aberrant expression of miRNAs is significantly correlated with the occurrence of immune thrombocytopenic purpura(ITP).The immune imbalance of M1/M2 macrophage contributes to the development of ITP.However,the role of miR-148b-3p in macrophage phenotype imbalance remains unknown in ITP.In this study,we aimed to explore whether miR-148b-3p inhibits M2 macrophage polarization in ITP and to investigate the underlying mechanism.Peripheral blood from 22 ITP patients were collected,and real-time PCR confirmed that miR-148b-3p was upregulated and Western blot analyses detected the expression of SOCS3 was down-regulated.Subsequent dualluciferase reporter gene assay indicated that miR-148b-3p could bind to SOCS3.Furthermore,we found significant correlation between miR-148b-3p expression and platelet count.Applying gain and lose the function experiments of miR-148b-3p and SOCS3,we demonstrated that suppression of miR-148b-3p or up-regulation of SOCS3 promoted macrophage M2 polarization by inhibiting JAK2/STAT3 pathway.Together,our findings demonstrate that that miR-148b-3p targeting SOCS3 inhibits M2 macrophage polarization via JAK2/STAT3 signaling in ITP.

Synthesis of carbon quantum dots decorating Bi_(2)MoO_(6) microspherical heterostructure and its efficient photocatalytic degradation of antibiotic norfloxacin

In this study,three-dimensional microspherical CQDs/Bi_(2)MoO_(6)heterostructures were synthesized using a simple alcohol-thermal method.It was found that the CQDs/Bi_(2)MoO_(6)had a large specific surface area of 56.0 m^(2)/g,and the introduction of CQDs extended the light absorption spectrum from 480 nm to 496 nm.When utilizing the synthesized CQDs/Bi_(2)MoO_(6)composite for photocatalytic degradation of antibiotic nor-floxacin in a water environment,complete decay of norfloxacin and effective removal of total organic car-bon(TOC)were achieved within 30 min.Through the optimization of material synthesis and experimen-tal conditions,the optimal CQDs loading amount was determined as 200μL,the optimal CQDs/Bi_(2)MoO_(6)dosage was 0.8 g/L.Moreover,the CQDs/Bi_(2)MoO_(6)worked well under a wide pH range of 4.4-10.8.The co-existence of HCO_(3)−enhanced the norfloxacin decay,while the presence of Cl^(−),NO_(3)^(−),and SO_(4)^(2−)slightly retarded it.The synthesized CQDs/Bi_(2)MoO_(6)had the great potential in removing and mineralizing nor-floxacin in real aquatic environments.

Waste-to-wealth:Functional biomass carbon dots based on bee pollen waste and application

Waste utilization is not only the protection of the environment and the practice of green chemistry,but also one of the ways to develop new materials.Herein,we report two biomass carbon dots which prepared from bee pollen waste by one-step hydrothermal method.The new two carbon dots were used in sensing,cell imaging and plant growth regulation.The differences in the structure and properties of the two carbon dots were evaluated by TEM,XPS,TG and various spectroscopic methods.Both two carbon dots contain abundant functional groups,polyatomic doping,excellent water solubility and stable photoluminescence.Due to these good properties,we have demonstrated its versatile applications in Fe3+sensing,cell imaging and plant growth regulation.It shows sensitive and specific Fe^(3+) responsiveness and good biocompatibility.This research provides a green and simple method for the recycling and reuse of bee pollen waste,and also provides a reference for the application of biomass carbon dots.

Comprehensive Humoral and Cellular Immune Responses to SARS-CoV-2 Variants in Diverse Chinese Population

The SARS-CoV-2 variants have been emerging and have made great challenges to current vaccine and pandemic control strategies.It is urgent to understand the current immune status of various Chinese populations given that the preexisting immunity has been established by national vaccination or ex posure to past variants.Using sera from 85 individuals(including 21 convalescents of natural infection,15 cases which suffered a breakthrough infection after being fully vaccinated,and 49 healthy vaccinees),we showed signifcantly enhanced neutralizing activities against SRAS-CoV-2 variants in convalescent sera,especially those who had been fully vaccinated.The neut ralizing antibodies against Omicron were detectable in 75%of convalescents and 4.9%of healthy vaccinees(p=0.006),with a GMT of 289.5,180.9-463.3,and 42.6,31.3-59,respetively.However,the neutralizing activities were weaker in young convalescents(aged<18y),with a detectable rate of 50%and a GMT of 46.4 against Omicron.We also examined and found no pan.sarbecovirus neutralizing activities in vaccinated SARS-CoV-I survivors.A booster dose could further increase the breadth and magnitude of neutralization against WT and variants of concern(VOCs)to different degrees.In addition,we showed that COVID-19-inactivated vaccines can elicit Omicron-specific T-cell responses.The positive rates of ELISpot reactions were 26.7%(4/15)and 43.8%(7/16)in the full vaccination group and the booster vaccination group,respectively,although without statistically significant difference.The neutralizing antibody titers declined while T-cell responses remain consistent over 6 months.These findings will inform the optimization of public health vaccination and intervention strategies to protect diverse populations against SARS-CoV-2 variants.Advances.Breakthrough infection significantly boosted neutralizing activities against SARS-CoV-2 variants a compared to booster immunization with inaclivated vaccine.Vaccine-induced vir us-specific T-cell immunity,on the other hand,may compensate for the shortall.Furthermore,the public health system should target the most vulnerable group due to a poorer protective serological response in both infected and vaccinated adolescents.