<strong>Purpose:</strong> The aim of the present study was to determine the degree of using floss and the demographic characteristics of medical faculties of Inner Mongolia Medical University. <strong&g...<strong>Purpose:</strong> The aim of the present study was to determine the degree of using floss and the demographic characteristics of medical faculties of Inner Mongolia Medical University. <strong>Materials and Methods:</strong> Participants completed the questionnaire in the classroom. These participants came from the medical faculties of Chinese Medicine and Western Medicine. These participants were interviewed to determine the demographic characteristics. Non-flossors responded according to their reasons for not flossing. <strong>Results:</strong> A total of 3481 effective data were available for analysis. The floss prevalence was 17.21%, which was lower by 5.72% for students living in rural areas, when compared to students living in urban areas. The largest proportion of non-flossing participants in rural areas answered, “I do not know what a floss is”, while participants in urban answered, “I do not want to use it”. The largest proportion of answered flossing frequency was less than once a month. <strong>Conclusion:</strong> The present study indicates that the prevalence of using floss in medical faculties in Inner Mongolia Medical University was higher, when compared to that in Sichuan province, China, but lower than that in other countries. Although the students use floss, more than one-third of students use floss less than once a month, which is close to “never”. The prevalence in rural areas is lower than those in urban areas. Male not only comprises the total number of prevalence, but also has a frequency greater than that in females. The emphasis on using floss should be improved in medical students.展开更多
<i><span style="font-family:Verdana;">Aconitum kusnezoffii</span></i><span style="font-family:Verdana;"></span><span style="font-family:;" "=&q...<i><span style="font-family:Verdana;">Aconitum kusnezoffii</span></i><span style="font-family:Verdana;"></span><span style="font-family:;" "=""><span style="font-family:Verdana;"> used as an analgesic and anti-inflammatory. It is often used not only in Mongolian medicine but also in traditional Chinese medicine. However, there are few studies on its impact of inflammatory cytokines. This study explored the effects of </span><span style="font-family:Verdana;"><i></i></span><i><i><span style="font-family:Verdana;">Aconitum kusnezoffii</span></i> <i><span style="font-family:Verdana;">in vitro</span></i><span style="font-family:Verdana;"></span></i></span><span style="font-family:Verdana;"> used human colon cancer (Caco-2) cells. Cytotoxicity was assessed by measuring effects on expression of IL-1</span><i><span style="font-family:Verdana;"><span style="white-space:nowrap;">β</span></span></i><span style="font-family:Verdana;">, IL-6, IL-8, and TNF-</span><i><span style="font-family:Verdana;"><span style="white-space:nowrap;">α</span></span></i><span style="font-family:Verdana;"> used enzyme-linked immun</span><span style="font-family:Verdana;">o</span><span style="font-family:;" "=""><span style="font-family:Verdana;">sorbent assay (ELISA). </span><span><span style="font-family:Verdana;"><i></i></span><i><i><span style="font-family:Verdana;">In vitro</span></i><span style="font-family:Verdana;"></span></i></span></span><span style="font-family:Verdana;"> study demonstrated that incubation of Caco-2 cells with some kinds of</span><i> </i><span style="font-family:Verdana;"><i></i></span><i><i><span style="font-family:Verdana;">Aconitum kusnezoffii</span></i><span style="font-family:Verdana;"></span></i><span style="font-family:Verdana;"> at some concentrations inhibit secretion of TNF-</span><i><span style="font-family:Verdana;"><span style="white-space:nowrap;">α</span></span></i><span style="font-family:Verdana;"> and FAXIU </span><span style="font-family:Verdana;"><i></i></span><i><i><span style="font-family:Verdana;">ACONITUM</span></i> <i><span style="font-family:Verdana;">KUSNEZOFFII</span></i><span style="font-family:Verdana;"></span></i><span style="font-family:Verdana;"> could inhibit the secretion of IL-1</span><i><span style="font-family:Verdana;"><span style="white-space:nowrap;">β</span></span></i><span style="font-family:Verdana;">. While IL-6 and IL-8 levels were unaffected after 24 h of treatment with </span><span style="font-family:Verdana;"><i></i></span><i><i><span style="font-family:Verdana;">Aconitum kusnezoffii</span></i><span style="font-family:Verdana;"><i></i></span><i><span style="font-family:Verdana;">, </span></i></i><span style="font-family:Verdana;"></span><span style="font-family:Verdana;"></span><span style="font-family:Verdana;">qujian </span><i><i><span style="font-family:Verdana;"><i></i></span><i><i><span style="font-family:Verdana;">Aconitum kusnezoffii</span></i><span style="font-family:Verdana;"><i></i></span><i><span style="font-family:Verdana;">, </span></i></i></i></i><span style="font-family:Verdana;"></span><span style="font-family:Verdana;"></span><span style="font-family:Verdana;"></span><span style="font-family:Verdana;">faxiu </span><i><i><i><i><span style="font-family:Verdana;"><i></i></span><i><i><span style="font-family:Verdana;">Aconitum kusnezoffii</span></i><span style="font-family:Verdana;"></span></i><span style="font-family:Verdana;">.</span></i></i></i></i>展开更多
Western blot analysis revealed that one IgG<sub>1</sub> monoclonal antibody (mAb) to sp18 family membrane proteins (Mr. 14, 16 and 18 ku) of bovine sperm reacted faintly with protein bands of 14,18, 22...Western blot analysis revealed that one IgG<sub>1</sub> monoclonal antibody (mAb) to sp18 family membrane proteins (Mr. 14, 16 and 18 ku) of bovine sperm reacted faintly with protein bands of 14,18, 22, 30 and 60 ku (reducing) in samples of mouse sperm. The mAb also reacted to protein of egg lysozyme. Using a laser confocal microscope, indirect immunofluorescence (IIF) showed that the sp18 antigens were present in the posterior head of murine sperm. In murine in vitro fertilization (IVF) and embryo development trails, a total of 426 oocytes from C<sub>57</sub>BL/6 and F<sub>1</sub> hybrid strain (CD<sub>1</sub>×C<sub>57</sub>BL/6 cross) of 12, female mice were used in 3 independent trails. After preincubating capacitated sperm with 182 μg/mL of sp18 mAb in the modified TYH IVF medium for 15—20 min, cumulus-oocyte-complexes were introduced. The fertilization rate in sp18 mAb groups was 77.1%, which was not significantly (P】0.05) different from the nonspecific mouse IgG(79.2 %) and non-IgG(80.3%) control groups. Fertilized oocytes had been展开更多
文摘<strong>Purpose:</strong> The aim of the present study was to determine the degree of using floss and the demographic characteristics of medical faculties of Inner Mongolia Medical University. <strong>Materials and Methods:</strong> Participants completed the questionnaire in the classroom. These participants came from the medical faculties of Chinese Medicine and Western Medicine. These participants were interviewed to determine the demographic characteristics. Non-flossors responded according to their reasons for not flossing. <strong>Results:</strong> A total of 3481 effective data were available for analysis. The floss prevalence was 17.21%, which was lower by 5.72% for students living in rural areas, when compared to students living in urban areas. The largest proportion of non-flossing participants in rural areas answered, “I do not know what a floss is”, while participants in urban answered, “I do not want to use it”. The largest proportion of answered flossing frequency was less than once a month. <strong>Conclusion:</strong> The present study indicates that the prevalence of using floss in medical faculties in Inner Mongolia Medical University was higher, when compared to that in Sichuan province, China, but lower than that in other countries. Although the students use floss, more than one-third of students use floss less than once a month, which is close to “never”. The prevalence in rural areas is lower than those in urban areas. Male not only comprises the total number of prevalence, but also has a frequency greater than that in females. The emphasis on using floss should be improved in medical students.
文摘<i><span style="font-family:Verdana;">Aconitum kusnezoffii</span></i><span style="font-family:Verdana;"></span><span style="font-family:;" "=""><span style="font-family:Verdana;"> used as an analgesic and anti-inflammatory. It is often used not only in Mongolian medicine but also in traditional Chinese medicine. However, there are few studies on its impact of inflammatory cytokines. This study explored the effects of </span><span style="font-family:Verdana;"><i></i></span><i><i><span style="font-family:Verdana;">Aconitum kusnezoffii</span></i> <i><span style="font-family:Verdana;">in vitro</span></i><span style="font-family:Verdana;"></span></i></span><span style="font-family:Verdana;"> used human colon cancer (Caco-2) cells. Cytotoxicity was assessed by measuring effects on expression of IL-1</span><i><span style="font-family:Verdana;"><span style="white-space:nowrap;">β</span></span></i><span style="font-family:Verdana;">, IL-6, IL-8, and TNF-</span><i><span style="font-family:Verdana;"><span style="white-space:nowrap;">α</span></span></i><span style="font-family:Verdana;"> used enzyme-linked immun</span><span style="font-family:Verdana;">o</span><span style="font-family:;" "=""><span style="font-family:Verdana;">sorbent assay (ELISA). </span><span><span style="font-family:Verdana;"><i></i></span><i><i><span style="font-family:Verdana;">In vitro</span></i><span style="font-family:Verdana;"></span></i></span></span><span style="font-family:Verdana;"> study demonstrated that incubation of Caco-2 cells with some kinds of</span><i> </i><span style="font-family:Verdana;"><i></i></span><i><i><span style="font-family:Verdana;">Aconitum kusnezoffii</span></i><span style="font-family:Verdana;"></span></i><span style="font-family:Verdana;"> at some concentrations inhibit secretion of TNF-</span><i><span style="font-family:Verdana;"><span style="white-space:nowrap;">α</span></span></i><span style="font-family:Verdana;"> and FAXIU </span><span style="font-family:Verdana;"><i></i></span><i><i><span style="font-family:Verdana;">ACONITUM</span></i> <i><span style="font-family:Verdana;">KUSNEZOFFII</span></i><span style="font-family:Verdana;"></span></i><span style="font-family:Verdana;"> could inhibit the secretion of IL-1</span><i><span style="font-family:Verdana;"><span style="white-space:nowrap;">β</span></span></i><span style="font-family:Verdana;">. While IL-6 and IL-8 levels were unaffected after 24 h of treatment with </span><span style="font-family:Verdana;"><i></i></span><i><i><span style="font-family:Verdana;">Aconitum kusnezoffii</span></i><span style="font-family:Verdana;"><i></i></span><i><span style="font-family:Verdana;">, </span></i></i><span style="font-family:Verdana;"></span><span style="font-family:Verdana;"></span><span style="font-family:Verdana;">qujian </span><i><i><span style="font-family:Verdana;"><i></i></span><i><i><span style="font-family:Verdana;">Aconitum kusnezoffii</span></i><span style="font-family:Verdana;"><i></i></span><i><span style="font-family:Verdana;">, </span></i></i></i></i><span style="font-family:Verdana;"></span><span style="font-family:Verdana;"></span><span style="font-family:Verdana;"></span><span style="font-family:Verdana;">faxiu </span><i><i><i><i><span style="font-family:Verdana;"><i></i></span><i><i><span style="font-family:Verdana;">Aconitum kusnezoffii</span></i><span style="font-family:Verdana;"></span></i><span style="font-family:Verdana;">.</span></i></i></i></i>
文摘Western blot analysis revealed that one IgG<sub>1</sub> monoclonal antibody (mAb) to sp18 family membrane proteins (Mr. 14, 16 and 18 ku) of bovine sperm reacted faintly with protein bands of 14,18, 22, 30 and 60 ku (reducing) in samples of mouse sperm. The mAb also reacted to protein of egg lysozyme. Using a laser confocal microscope, indirect immunofluorescence (IIF) showed that the sp18 antigens were present in the posterior head of murine sperm. In murine in vitro fertilization (IVF) and embryo development trails, a total of 426 oocytes from C<sub>57</sub>BL/6 and F<sub>1</sub> hybrid strain (CD<sub>1</sub>×C<sub>57</sub>BL/6 cross) of 12, female mice were used in 3 independent trails. After preincubating capacitated sperm with 182 μg/mL of sp18 mAb in the modified TYH IVF medium for 15—20 min, cumulus-oocyte-complexes were introduced. The fertilization rate in sp18 mAb groups was 77.1%, which was not significantly (P】0.05) different from the nonspecific mouse IgG(79.2 %) and non-IgG(80.3%) control groups. Fertilized oocytes had been