Preprocessing and Efficacy Analysis of Comprehensive Anti-Inflammatory Treatment for Lymphedema in Patients with Irritating Contact Dermatitis

Objective: To explore the pre-treatment and efficacy analysis of comprehensive anti-inflammatory treatment for lymphedema in patients with irritating contact dermatitis. Method: Convenience sampling method was used to observe the skin of 160 patients with upper limb lymphedema admitted to the lymphedema outpatient department of our hospital. They were divided into an observation group (80 cases) and a control group (80 cases), and both groups received a course of comprehensive anti-inflammatory treatment (20 treatments). The control group received routine skin care;On the basis of the control group, the observation group received pre-treatment of the affected limb skin: Laofuzi herbal ointment was applied externally to the prone areas of irritating contact dermatitis (such as the upper arm, inner forearm, and cubital fossa). Result: The incidence of irritating contact dermatitis in the observation group was significantly lower than that in the control group (P 0.05). Patients in the observation group felt significantly better in terms of comfort, skin moisture, and itching relief after being wrapped with low elasticity bandages than those in the control group (P Conclusion: Preventive treatment can effectively reduce the incidence of irritating contact dermatitis, prolong the time of stress treatment, thereby increasing efficacy and improving patient compliance.

Brønsted-acid sites induced photocatalytic cracking of low-polarity polyethylene plastics

Polyolefins such as polyethylene(PE)are one of the largest-scale synthetic plastics and play a key role in modern society.However,polyethylene is extremely inert to chemical recycling owing to its lack of chemical functionality and low polarity,making it one of the most challenging environmental hazards globally.Herein,we developed a phosphorylated CeO_(2)catalyst by an organophosphate precursor and featured efficient photocatalysis of low-density polyethylene(LDPE)without the acid or alkaline pre-treatment.Compared to pristine CeO_(2),the surface phosphorylation allows to introduce Brønsted acid sites,which facilitate to form carbonium ions on LDPE via protonation.In addition,the suitable band structure of the phosphorylated CeO_(2)catalyst enables efficient photoabsorption and generates reactive oxygen species,leading to the C–C bond cleavage of LDPE.As a result,the phosphorylated CeO_(2)catalyst exhibited an outstanding carbon conversion rate of>94%after 48 h of photocatalysis under 50 mW/cm^(2)of simulated sunlight,with a high CO_(2)product selectivity of>99%.Furthermore,the PE microparticles with sizes larger than 10μm released from LDPE plastic wrap were directly and completely degraded by photocatalysis within 12 h,suggesting an attractive and environmentally benign strategy of utilizing solar energy-based photocatalysis for reducing potential hazards of LDPE plastic trashes.

Residual stress modeling of mitigated fused silica damage sites with CO_(2)laser annealing

A numerical model based on measured fictive temperature distributions is explored to evaluate the residual stress fields of CO_(2)laser-annealed mitigated fused silica damage sites.The proposed model extracts the residual strain from the differences in thermoelastic contraction of fused silica with different fictive temperatures from the initial frozen-in temperatures to ambient temperature.The residual stress fields of mitigated damage sites for the CO_(2)laser-annealed case are obtained by a finite element analysis of equilibrium equations and constitutive equations.The simulated results indicate that the proposed model can accurately evaluate the residual stress fields of laser-annealed mitigated damage sites with a complex thermal history.The calculated maximum hoop stress is in good agreement with the reported experimental result.The estimated optical retardance profiles from the calculated radial and hoop stress fields are consistent with the photoelastic measurements.These results provide sufficient evidence to demonstrate the suitability of the proposed model for describing the residual stresses of mitigated fused silica damage sites after CO_(2)laser annealing.

胃肠间质瘤术前靶向治疗后的病理学评估与影像学评估的一致性分析

目的:探讨Choi标准及病理学效应对胃肠间质瘤(gastrointestinal stromal tumor, GIST)术前靶向治疗后疗效及预后的评估价值,并观察两者的一致性。方法:回顾性分析于昆明医科大学第三附属医院收治37例术前伊马替尼(imatinib,IM)靶向治疗GIST患者的临床病理资料,Kaplan-Meier法和Log-rank检验对Choi标准及病理学效应进行生存分析,Spearman等级相关及Kappa检验分析Choi标准与病理学效应的一致性。结果:37例患者术前中位治疗时间为10(2~36)个月。Choi标准评估无完全缓解(complete response,CR)病例,部分缓解(partial response,PR)26例,疾病稳定(stable disease,SD)5例,疾病进展(progressive disease,PD)6例;Choi标准疗效有效组(CR+PR)与无效组(SD+PD)之间总生存期的差异具有统计学意义(P<0.01)。病理学效应评估完全效应1例,高度效应11例,部分效应18例,零级效应7例;病理效应有效组(完全效应+高度效应+部分效应)与无效组(零级效应)之间总生存期的差异具有统计学意义(P<0.01)。Choi标准与病理学效应有相关性(r=0.592,P<0.01)及中度一致性(κappa=0.566)。结论:Choi标准与病理学效应有中等程度的相关性及一致性,两者均可作为胃肠间质瘤术前靶向治疗疗效评估及预后判断的参考指标,联合应用两者评估具有更高的临床应用价值。

miR-30a-5p/PHTF2 axis regulates the tumorigenesis and metastasis of lung adenocarcinoma

Background:Lung adenocarcinoma is a very pervasive histological form of lung cancers,and inhibiting metastasis is crucial for effective treatment.In this investigation,we explored the functional interaction of miR-30a-5p and the putative transcription factor 2 of the homeodomain(PHTF2)in dictating the aggressiveness and metastasis of lung adenocarcinoma.Method:We collected clinical samples to evaluate the expression patterns of miR-30a-5p and PHTF2 in lung adenocarcinoma along with normal tissues.Cellular experiments including cell count kit(CCK)-8 growth assay,apoptosis analysis,migration and invasion examinations were performed to assess the aggressiveness of lung adenocarcinoma cells.Furthermore,we examined tumorigenesis and metastasis in a nude mouse model.Results:MiR-30a-5p exhibited downregulation pattern in lung adenocarcinoma samples.Transfection of miR-30a-5p mimic in lung adenocarcinoma cells resulted in the suppression of malignant characteristics.Notably,the administration of miR-30a-5p mimic also curbed tumorigenesis and metastasis of lung adenocarcinoma cells in animal model.Moreover,PHTF2 was found to be a molecular target of miR-30a-5p.Upregulating PHTF2 counteracted the tumor-suppressive effect of the miR-30a-5p mimic.Conclusion:miR-30a-5p functions as a tumor-suppressive molecule while PHTF2 acts as an oncogenic factor in the development and metastasis of lung adenocarcinoma.Therefore,targeting miR-30a-5p and PHTF2 could be developed into a promising therapeutic approach for inhibiting metastasis in lung adenocarcinoma.

Effects of Alexithymia to Stigma of Patients with Lymphedema

BACKGROUND: Secondary lymphedema is one of the common complications after malignant tumor surgery. It is a chronic and complex disease. Once lymphedema occurs, there will be discomfort such as limb swelling, pain, numbness and tension, which will eventually lead to changes in the appearance of the affected limb and will seriously affect the quality of life and require lifelong treatment and psychosocial support. This study investigated the current situation of stigma and alexithymia in patients with lymphedema, and discussed the impact of alexithymia on stigma in patients with lymphedema. AIMS: To understand the current situation of stigma and alexithymia in patients with lymphedema, and to analyze the influence of alexithymia on stigma. METHODS: 195 patients with lymphedema in a hospital were selected by convenient sampling. General information questionnaire, Toronto Alexithymia Scale and social impact scale were used to investigate respectively, to study the general situation, stigma and alexithymia of the respondents. RESULTS: The results showed that the total score of stigma in 195 patients with lymphedema was (60.36 ± 11.08), and the total score of alexithymia was (56.53 ± 8.43). Multiple linear regression analysis showed that alexithymia and family relationship were the influencing factors of stigma in patients with lymphedema. CONCLUSIONS: The patients with lymphedema have obvious stigma, and alexithymia and family relationship are the influencing factors.

用于循环肿瘤细胞定量分析的CK19数字PCR检测方法的建立及性能验证

本研究拟建立检测CK19基因表达的数字PCR(digital PCR,dPCR)方法并测试其性能,以期利用该方法的超高敏感性和绝对定量优势进行循环肿瘤细胞(circulating tumor cells,CTC)的定量分析。首先,根据CK19基因的mRNA序列进行引物探针设计,以看家基因ABL1作为内参,采用人乳腺癌MCF7细胞和健康人白细胞从13套引物探针之中筛选出了最佳的引物探针,测序结果证实扩增序列无误。其次,针对选定的引物探针进行反应条件的优化,以不同浓度的健康人白细胞cDNA为模板进行空白检测限(limit of blank,LOB)的分析,证实当ABL1基因拷贝数为20,000、15,000、10,000、5000、2500时,CK19的LOB分别为9.24、8.93、3.12、3.17和2.53拷贝。再次,采用MCF7细胞和健康人白细胞的cDNA模板进行不同浓度预混的检测限(limit of detection,LOD)分析,证实50%、10%、5%、1%、0.5%和0.1%的浓度配比下均可以有效检出CK19基因,线性R2值为0.9998。最终,临床样本的数字PCR检测结果发现晚期乳腺癌患者的CK19拷贝数高于健康人对照。上述结果说明本研究所建立的检测CK19基因的数字PCR方法具有敏感、特异和定量准确等优势,未来经过进一步验证之后有望用于乳腺癌的CTC定量分析,具有良好的应用前景。

Anchoring nitrogen-doped Co_(2)P nanoflakes on NiCo_(2)O_(4)nanorod arrays over nickel foam as high-performance 3D electrode for alkaline hydrogen evolution

Effective and robust electrocatalysts are mainly based on innovative materials and unique structures.Herein,we designed a flakelike cobalt phosphide-based catalyst supporting on NiCo_(2)O_(4)nanorods array,which in-situ grew on the nickel foam(NF)current collector,referring as NCo_(2)P/NiCo_(2)O_(4)/NF electrode.By optimizing the microstructure and electronic structure through 3D hierarchy fabrication and nitrogen doping,the catalyst features with abundant electrochemical surface area,favorable surface wettability,excellent electron transport,as well as tailored d band center.Consequently,the as-prepared N-Co_(2)P/NiCo_(2)O_(4)/NF electrode exhibits an impressive HER activity with a low overpotentials of58 mV at 10 mA cm^(-2),a Tafel slop of 75 mV dec^(-1),as well as superior durability in alkaline medium.This work may provide a new pathway to effectively improve the hydrogen evolution performance of transition metal phosphides and to develop promising electrodes for practical electrocatalysis.

Effect of dual targeting procyanidins nanoparticles on metabolomics of lipopolysaccharide-stimulated inflammatory macrophages

Inflammation plays an important role in the occurrence and development of many inflammatory diseases.The purpose of this study was to evaluate the anti-inflammatory effect and metabolic behavior of the dual targeting procyanidins(PC)nanoparticles on lipopolysaccharide(LPS)-stimulated inflammatory macrophages by metabolomics method.The double-targeting PC nanoparticles could specifi cally target both the CD44 receptor and mitochondria,while the single targeting PC-loaded nanoparticles that could target the CD44 receptor on the surface of macrophages.The double-targeting PC nanoparticles had better inhibitory effect than single-targeting PC nanoparticles on the leakage of lactate dehydrogenase and reactive oxygen species overexpression induced by LPS.Amino acid metabolism,energy metabolism and purine metabolism were disordered in LPS-treated group,and metabolic pathway analysis indicated that the double-targeting PC nanoparticles reversed some of LPS impacts.The changes of these potential biomarkers and their corresponding pathways are helpful to further understand the mechanism of PC nanoparticles in alleviating inflammation,and promote their application in nutrition intervention.

凝血因子Ⅲ在非小细胞肺癌高凝状态患者外周血中的表达及其临床意义

目的:研究凝血因子Ⅲ在非小细胞肺癌(non-small cell lung cancer,NSCLC)高凝状态患者外周血中的表达水平,并探讨其表达水平与临床病理特征和生存期的关系。方法:选取2013年1月至2014年10月云南省肿瘤医院病理和生化检测证实为NSCLC高凝状态患者74例,NSCLC无高凝状态患者42例作为研究对象,酶联免疫法检测血清凝血因子Ⅲ的表达水平,分析其与NSCLC临床病理特征和预后的关系。结果:化疗前NSCLC高凝状态患者血清凝血因子Ⅲ为(560.32~200.34)ng/L,显著高于无高凝状态患者(463.29~159.22)ng/L的表达水平(P=0.008),且显著高于化疗后凝血因子Ⅲ(471.39±160.31)ng/L的血清表达水平(P<0.001);凝血因子Ⅲ在NSCLC高凝状态患者血清中的表达水平与淋巴结转移(P=0.026)、远端转移(P=0.025)和TNM分期(P=0.007)有关,且与凝血酶原时间(PT)(r=-0.638,P=0.032)、活化部分凝血酶时间(APTT)(r=-0.702,P=0.028)呈负相关,与纤维蛋白原(FIB)(r=0.715,P=0.008)、血小板(PLT)(r=0.597,P=0.007)呈正相关;凝血因子Ⅲ高表达水平的NSCLC高凝状态患者1~3年总生存期显著低于低表达患者(P<0.05)。结论:NSCLC高凝状态患者血清凝血因子Ⅲ表达水平与淋巴结转移和TNM分期有关,对患者生存期具有预测的指导意义。

Inverted T-Shaped Flap Nursing after the Reverse Double V-Shaped Mastoconstriction Suspension for Breast Cancer

Objective: To explore the inverted T-shaped flap nursing after breast-conserving surgery combined with the reverse double V-shaped mastoconstriction suspension for breast cancer, and summarizes the nursing methods and experience. Method: 5 patients who underwent reverse double V-shaped mastoconstriction suspension and formed inverted T-shaped flap incision were selected from the same medical group in the Department of Breast Cancer of Sun Yat-sen University’s Cancer Center from February 1, 2019 to April 30, 2019, and they were evaluated for the recovery of blood supply of skin flap, sensory function of nipple and areola, breast satisfaction, etc. Results: Among the 5 patients in this group, the flaps of them all survived smoothly. The one-year follow-up after the operation showed that they were satisfied with the appearance of the breast after the operation, with normal sensory function, and no secondary sagging. Conclusion: The most important thing about the inverted T-shaped flap after the reverse double V-shaped mastoconstriction suspension for breast cancer is the blood supply. Close observation of the condition of the skin flap after the operation, strengthening the care of the skin flap, and preventing infection are the keys to the success of the operation.

Factors Influencing Readiness for Hospital Discharge among Patients Undergoing Breast Cancer Surgery

Background: Breast cancer has become an important disease burden harmful to women’s health. The treatment of breast cancer is mainly surgical treatment. In the context of enhanced recovery after surgery, the average length of hospital stay has been reduced, so there is increasing concern about postoperative patient discharge readiness. Objective: To identify factors influencing readiness for hospital discharge among patients having undergone breast cancer surgery and to explore the relationship between discharge readiness and the quality of discharge teaching. Methods: A total of 99 patients after breast cancer surgery at a regional tertiary medical tumor center in Guangzhou between June 2021 and December 2021 were collected using the convenience sampling method. A general data questionnaire, readiness for hospital discharge scale, and quality of discharge teaching scale were used to conduct a questionnaire survey on the subjects. Results: 83.8% of the patients feel ready for discharge, and the score of discharge readiness was 155.98 ± 28.87. The patients’ content received and the skills in teaching delivery were the influencing factors of discharge readiness. Discharged with a drainage tube was an independent factor of discharge readiness. Conclusions: Readiness for hospital discharge among patients undergoing breast cancer surgery was above medium level, and perfect intervention measures should be formulated for the influencing factors of discharge readiness. Especially, specific executable safeguard measures should be formulated for patients who are discharged early after surgery to promote the safety of home care.

Comprehensive Online Management Based on Cloud Platform for Breast Cancer Patients Using Dual-Targeted Therapy with Macromolecular Monoclonal Antibodies

Objective: The purpose of the study was to explore the application effects of the cloud platform-based comprehensive online management for breast cancer patients using dual-targeted therapy with macromolecular monoclonal antibodies. Methods: 120 breast cancer patients treated by dual-targeted therapy with macromolecular monoclonal antibodies were managed by a cloud platform from March to November 2019. Comprehensive online management included consultation about drugs and side effects and frequently asked questions in the dual-targeted therapy with macromolecular monoclonal antibodies. Results: In the consultation about drugs and side effects, there were five patients with fever, neutrophil, cough, and fatigue;24 with diarrhea;25 with nausea;11 with oral mucosal inflammation;10 with rashes and dry skin;8 with insomnia;and 1 with palpitation. Moreover, 110 patients with anxiety about the missed or delayed treatment were properly handled. Conclusion: The comprehensive online management of dual-targeted therapy with macromolecular monoclonal antibodies based on the cloud platform is helpful to satisfy the at-home breast cancer patients’ needs, ensure the continuity of dual-targeted therapy with macromolecular monoclonal antibodies for breast cancer patients, prevent misinformation, alleviate patients’ negative psychological emotions, and reduce patients’ economic losses. The online cloud platform integrated management model is crucial for managing patients with breast cancer treated by dual-targeted therapy.

后非洲猪瘟时期洋三元(DLY)商品母猪作种用的可行性分析及建议

自从2018年非洲猪瘟在中国暴发以来,为了尽快恢复产能,我国很多猪场在生产上开始采用洋三元商品母猪留作种用。通过收集某大型猪场的二元(LY)母猪和洋三元(DLY)商品母猪的生产记录,统计两者在生产成绩上的差异,并综合其他文献来分析洋三元商品母猪留作种用的优缺点,给出改进的措施,为后非洲猪瘟时期养猪生产提供参考。

Evaluation of the Preventive Effect of Regional Cooling Nursing on Hand Foot Syndrome Caused by Doxorubicin Hydrochloride Liposome

Purpose: To explore the preventive effect of Regional cooling comprehensive nursing on hand foot syndrome caused by pegylated liposomal doxorubicin (PLD). Method: Adopt overall sampling method. Patients who used the same adjuvant drugs from January to December 2020 were randomly divided into an intervention group and a control group. The patients in the two groups received routine nursing guidance and drug prevention for the use of amygdalin. The patients in the intervention group were required to take protective measures of Regional cooling during chemotherapy. The occurrence of hand foot syndrome during adriamycin liposome administration was compared between the two groups. Results: By comparing the adverse reactions of cases during Adriamycin Administration, the incidence rates of grade I, II and III hand foot syndrome in the control group were 28.8%, 7.6% and 27.5% respectively, and the incidence rates of grade I, II and III hand foot syndrome in the intervention group were 42.1%, 12.3% and 7.0% respectively, with statistical significance (P Conclusion: Regional cooling nursing and preventive behavior guidance can effectively reduce the severity of hand foot syndrome caused by adriamycin.

Efficacy of Manual Lymphatic Drainage for Breast Cancer-Related Lymphedema

Purpose: The effect of manual lymphatic drainage in patients with breast cancer is controversial. The purpose of this study is to evaluate the role of manual lymphatic drainage (MLD) in breast cancer-related lymphedema treatment. Methods: The electronic databases of EMBASE, PubMed, Web of Science, and The Cochrane Library were searched to find English articles on MLD which were published before January 2020. After two evaluators selected the studies and independently evaluated literature quality, meta-analysis was carried out with RevMan 5.3 software. The outcome index of lymphedema treatment changed in edema volume. Results: The study included six RCTs of 364 patients and the meta-analysis showed no significant difference in the effect of MLD for BCRL compared with other treatments (mean difference, 3.76;95% confidence interval, -35.09 to 42.62;Z, 0.19;p = 0.850). Conclusion: MLD can relieve the body tissue, rapidly improve local condition, and enhance complete decongestive therapy (CDT) efficacy. MLD can prevent BCRL and improve the symptoms of stage I lymphedema. It should be widely applied to prevent BCRL from entering an irreversible state.

Cognitive Impairment Related to Chemotherapy in 60 Patients with Advanced Gastric Cancer

Background: Gastric cancer is one of the most common malignant tumors in the world. In recent years, studies on chemotherapy-induced cognitive impairment have been reported mainly on young women with breast cancer. Only few studies on chemotherapy-related cognitive impairment in patients with gastric cancer. Objective: To investigate the effect of chemotherapy on cognitive function in patients with advanced gastric cancer. Methods: Sixty patients with stage IV gastric cancer who received fluorouracil based chemotherapy were selected as gastric cancer group, including 25 patients with S-1 (S-1 group) and 35 patients with capecitabine (capecitabine group). 60 healthy controls matched with age and education level were recruited as control group. Mini Mental State Examination (MMSE), Montreal Cognitive Assessment (MoCA) and Auditory Verbal Learning Test (AVLT) were used to test. Results: There was no significant difference in MMSE, MoCA and AVLT between healthy control group and gastric cancer group before chemotherapy. MMSE, MoCA, immediate memory and delayed memory decreased in gastric cancer patients after chemotherapy (t = 2.206, t = 2.399, t = 2.206, t = 2.654, P Conclusion: Patients with gastric cancer will have chemotherapy-related cognitive dysfunction after chemotherapy. It is necessary to evaluate and guide or treat patients with cognitive function, so as to improve the quality of life of patients.

覆盖面、水和缺陷对邻苯二酚/二氧化钛界面性质的影响

邻苯二酚/二氧化钛界面是一种研究染料敏化太阳能电池相关性能的简单模型,然而,水和缺陷的存在对邻苯二酚/二氧化钛界面电子能级和激子性质的影响却很少被探究.本文通过第一性的多体格林函数理论研究四种邻苯二酚/二氧化钛界面,确定覆盖面、水和缺陷对电子能级和激子性质的影响.邻苯二酚在金红石(110)表面的吸附使二氧化钛的最高价带和最低导带的能量增加了约0.7 eV,覆盖面的增加和水的存在降低了强度最大的电荷转移态激子的光学吸收.当吸附基底为还原的羟基化二氧化钛时,最低导带大大降低,导致亚带隙变为2.51 eV.四种界面结构的激子分布离域至几个原胞,特别是吸附基底为羟基化二氧化钛的界面结构.采用羟基化的二氧化钛基底时虽然会降低开路电压,但同时也增加了光生电子与空穴的分离,因此可通过控制缺陷浓度来提高光伏效率.

固态氧化物电解池中碳-分子电化学转化为可再生燃料

近年来,随着社会环保意识的迅速提高以及对可再生能源利用能力的大幅增强,以燃料电池和电解池为代表的电化学技术已经逐渐在能源的存储、转化和利用方面发挥着不可或缺的独特作用.其中,固态氧化物电解池经过多年的发展,在装置成本和工作效率上取得了长足的进步,在储能转化方面具有重要的潜力.与此同时,伴随着《巴黎协定》签订以来各国的“碳中和”路线图逐渐出台,利用相对廉价易得的可再生电能,将二氧化碳(CO_(2))和甲烷(CH_(4))等碳-(C1)分子电解转化为高附加值的可再生燃料(如水煤气、乙烯等),对于碳中和目标的实现具有重要的意义.因此,C1分子电化学转化的研究成为了当下重点关注的研究领域,许多重要的研究成果和技术进步在过去几年中不断涌现.固态氧化物电解池作为一种代表性的C1分子电解和转化平台,也日渐引起相关领域研究人员的关注和兴趣.与传统的C1分子催化转化方法相比,基于固态氧化物电解池的电解转化技术具有两个重要优点:高能量转换效率与体系抗中毒能力.这两个特性作为体系稳健性的基石,保障了C1分子转化为可再生燃料的反应过程的长期可持续性.本文首先简要回顾了固态氧化物电解池的前沿技术与发展,并从电解池系统分类、反应体系的特征和反应体系发展的前景与挑战这三个方面,简要介绍了近年来基于固态氧化物电解池体系的C1分子电化学转化的代表性工作.CO_(2)与CH_(4)作为廉价易得的C1分子的代表,其转化因其反应分子惰性及反应过程不可控性而广受研究者关注,本文重点关注了在固态氧化物电解池中CO_(2),CO_(2)/H_(2)O和CH_(4)三个体系的电化学反应过程和近期研究进展,希望可为相关研究人员未来设计更合适的催化剂和构建更优的电解池结构提供有益的参考.本文还针对目前固态氧化物电解池体系在C1分子转化领域所面临的挑战,提出了未来的一些可能的研究方向,以期助力研究者在不远的将来实现C1分子电解生产可再生燃料的实用化.

Investigation of anaplasmosis in Yiyuan County,Shandong Province,China

Objective:To investigate the situation of anaplasmosis in Yiyuan county.Shandong Province. Methods:A total of 26 blood samples from febrile patients suspected of anaplasmosis,48 blood samples from healthy farmers,8 from dogs,and 10 from goats and 170 ticks were collected in the same area during 2005-2007,and detected by serological and molecular methods.Results: Eight confirmed cases and 6 probable cases were determined using serologic and molecular methods.The seroprevalence of Anaplasma phagocytophilum(A.phagocytophilum) was 26.7% in healthy cases.Nine out of 10 sheep samples and 7 out of 8 dog samples reacted positively to the A.phagocytophilum antigen.PCR amplification and sequencing of the 16SrRNA of,4. phagocytophilum gene showed that some samples from patients,goats and ticks were 100% identical.The seroprevalence of Rickettsia typhi was 22.9%,Orientia tsutsugamushi 6.3%, Rickettsia sibirica 27.1%,Coxiella burnetii 18.8%,Bartonella henselae 31.3%,and Borrelia burgdorferi 41.6%.Conclusions:It is important to make differential diagnosis of febrile patients and to apply treatment with specific antibiotics.It is needed to enforce essential prevention and control measures including tick control and to improve sanitation conditions.