Scar-free wound healing is a challenging process due to the excessive deposition of extracellular matrix and collagen.To overcome this issue,hydrogels with superior biochemical and mechanical properties have been used...Scar-free wound healing is a challenging process due to the excessive deposition of extracellular matrix and collagen.To overcome this issue,hydrogels with superior biochemical and mechanical properties have been used in combination with medicinal compounds as wound dressings.In this study,a novel composite hydrogel consisting of double-crosslinked photocurable hyaluronic acid methacrylate(HAMA)and Laponite(Lap)loaded with bioactive bone morphogenetic protein 4(BMP4)was developed and thoroughly characterized for its properties such as degradation,morphology,porosity,compression,skin adhesion and load release.The effect of the HAMA/Lap/BMP4 hydrogel was evaluated through both in vitro and in vivo experiments.In the in vivo rabbit ear-scar model,the HAMA/Lap/BMP4 hydrogel dressing was found to reduce scar-related expressions ofα-SAM and decrease the ratio of collagenΙ/III in wounded tissue.Additionally,histopathological examination indicated that the HAMA/Lap/BMP4 hydrogel-treated groups exhibited enhanced wound repair and increased levels of collagen maintenance compared to other standard groups,ultimately leading to scarless wound healing.Therefore,this sustained-release photocurable HAMA/Lap/BMP4 hydrogel offers a therapeutic approach for scar-free wound healing.展开更多
To regulate cell behaviors and promote nerve function recovery,three-dimensional(3D)conductive hydrogel can transmit intercellular electrical signals,and effectively provide the cell survival environment.However,produ...To regulate cell behaviors and promote nerve function recovery,three-dimensional(3D)conductive hydrogel can transmit intercellular electrical signals,and effectively provide the cell survival environment.However,produc-ing hydrogels with simultaneous high conductivity,favorable biocompatibility,and tissue-matching properties remains a challenge for spinal cord injury(SCI)treatment.Here,a conductive,multifunctional,and biocompati-ble VPImBF4 ionic liquid(IL)with photosensitive chitosan-based hydrogel(pCM@IL)is developed.The pCM@IL hydrogel exhibits a 3D microporous structure that could maintain cell viability and improve cell growth.Elas-tic modulus,conductivity,and biodegradability of the pCM@IL hydrogels are investigated with tissue-matching mechanical properties.The pCM@IL conductive hydrogels synergistically enhance neuronal cell proliferation and promote neuronal cells differentiation via upregulates synapse gene(Tubulin𝛽3,GAP43,Synaptophysin)expression.Furthermore,in vivo studies of the pCM@IL conductive hydrogels as implants demonstrate low-inflammation and neovascularize promotion and appropriate biodegradable properties.The developed pCM@IL conductive hydrogel is a promising therapeutic scaffold biomaterial for SCI repair.展开更多
基金supported by the Natural Science Foundation of Hubei Province[2022CFB386].
文摘Scar-free wound healing is a challenging process due to the excessive deposition of extracellular matrix and collagen.To overcome this issue,hydrogels with superior biochemical and mechanical properties have been used in combination with medicinal compounds as wound dressings.In this study,a novel composite hydrogel consisting of double-crosslinked photocurable hyaluronic acid methacrylate(HAMA)and Laponite(Lap)loaded with bioactive bone morphogenetic protein 4(BMP4)was developed and thoroughly characterized for its properties such as degradation,morphology,porosity,compression,skin adhesion and load release.The effect of the HAMA/Lap/BMP4 hydrogel was evaluated through both in vitro and in vivo experiments.In the in vivo rabbit ear-scar model,the HAMA/Lap/BMP4 hydrogel dressing was found to reduce scar-related expressions ofα-SAM and decrease the ratio of collagenΙ/III in wounded tissue.Additionally,histopathological examination indicated that the HAMA/Lap/BMP4 hydrogel-treated groups exhibited enhanced wound repair and increased levels of collagen maintenance compared to other standard groups,ultimately leading to scarless wound healing.Therefore,this sustained-release photocurable HAMA/Lap/BMP4 hydrogel offers a therapeutic approach for scar-free wound healing.
基金supported by National Key Research and Development Program of China(No.2016YFC1101302)self-determined and innovative research funds of WUT(S202010497265)+1 种基金A.K.Y.acknowledges the Royal Soci-ety Research Grant(RGS\R2\202305)G.Y.and N.J.acknowledge the Fundamental Research Funds for the Central Universities.
文摘To regulate cell behaviors and promote nerve function recovery,three-dimensional(3D)conductive hydrogel can transmit intercellular electrical signals,and effectively provide the cell survival environment.However,produc-ing hydrogels with simultaneous high conductivity,favorable biocompatibility,and tissue-matching properties remains a challenge for spinal cord injury(SCI)treatment.Here,a conductive,multifunctional,and biocompati-ble VPImBF4 ionic liquid(IL)with photosensitive chitosan-based hydrogel(pCM@IL)is developed.The pCM@IL hydrogel exhibits a 3D microporous structure that could maintain cell viability and improve cell growth.Elas-tic modulus,conductivity,and biodegradability of the pCM@IL hydrogels are investigated with tissue-matching mechanical properties.The pCM@IL conductive hydrogels synergistically enhance neuronal cell proliferation and promote neuronal cells differentiation via upregulates synapse gene(Tubulin𝛽3,GAP43,Synaptophysin)expression.Furthermore,in vivo studies of the pCM@IL conductive hydrogels as implants demonstrate low-inflammation and neovascularize promotion and appropriate biodegradable properties.The developed pCM@IL conductive hydrogel is a promising therapeutic scaffold biomaterial for SCI repair.
