Study on Quality Standards of Children Antipyretic Lotion

[Objectives]To establish suitable quality standards of children antipyretic lotion.[Methods]Qualitative identification was performed for Paederia scandens and Artemisia carvifolia in Children Antipyretic Lotion by thin-layer chromatography(TLC),and the content of paederosidic acid in children antipyretic lotion was determined by high performance liquid chromatography(HPLC).Besides,chromatographic column Feinigen XPeonyx C18(250 mm×4.6 mm,5μm),mobile phase was used,gradient elution was carried out with acetonitrile-0.1%phosphoric acid aqueous solution,the detection wavelength was 236 nm,the flow rate was 1.0 mL/min,the column temperature was 30℃,and the injection volume was 10μL.[Results]The thin-layer chromatography of P.scandens and A.carvifolia reference materials showed clear spots,good separation effect,strong specificity,and no interference in the limit control;paederosidic acid showed a good linear relationship in the range of 0.65-9.75μg(R2=0.9996),the average sample recovery rate was 99.72%(RSD=2.52%).[Conclusions]The qualitative and quantitative methods established in this experiment are simple,reproducible,and the results are accurate and reliable,and can be applied as a quality control method for children antipyretic lotions.

Study on the Quality Standard of Shema Mixture

[Objectives]To establish the quality standard of Shema mixture.[Methods]Thin-Layer Chromatography(TLC)was used to qualitatively identify Ephedra sinica Stapf,Belamcanda chinensis(L.)Redoutéand Citrus maxima(Burm.)Merr.cv.Tomentosa in the mixture,and High Performance Liquid Chromatography(HPLC)was established for the determination of the content of ephedrine hydrochloride,which was an effective component of principle drug E.sinica Stapf in the mixture.[Results]TLC was used to identify E.sinica Stapf,B.chinensis(L.)Redoutéand C.maxima(Burm.)Merr.cv.Tomentosa in Shema mixture,and the characteristic spots were stable and clear.Ephedrine hydrochloride had a good linear relationship in the range of 2.01-14.06μg,the average recovery of samples was 98.77%,RSD=1.58%.[Conclusions]TLC and HPLC,as the quality control methods of Shema mixture,have high accuracy and good repeatability,which laid a foundation for the quality control of Shema mixture.