Objective:To establish immunomodulatory potential of aqueous extract of Polygonum minus(P.minus)leaves,the haematological effects and lysozyme activity of aqueous extract of P.minus leaf on Clarias gariepinus was stud...Objective:To establish immunomodulatory potential of aqueous extract of Polygonum minus(P.minus)leaves,the haematological effects and lysozyme activity of aqueous extract of P.minus leaf on Clarias gariepinus was studied.Methods:The fish were grouped and administered with the P.minus aqueous leaf extract intraperitoneally.Blood and serum samples were collected from each group and examined for various blood parameters.The turbidimetric assay for lysozyme activity using M.luteus and the survival rate of fish against A.hydrophila was carried out.Results:There was no significant impact(P>0.05)on white blood cell count,red blood cell count,mean corpuscular volume,mean corpuscular hemoglobin,mean corpuscular hemoglobin concentration and lysozyme activity was significantly(P<0.05)higher than control.The serum glutamic oxaloacetic transaminase and serum glutamic pyruvic transaminase levels were significantly lower than control(P<0.05).The highest dose group showed longest survival.Conclusions:From this study,it is considered that the aqueous extract of P.minus can be used as an immunostimulant in African catfish,but continuous administration may require maintaining the protection.展开更多
文摘Objective:To establish immunomodulatory potential of aqueous extract of Polygonum minus(P.minus)leaves,the haematological effects and lysozyme activity of aqueous extract of P.minus leaf on Clarias gariepinus was studied.Methods:The fish were grouped and administered with the P.minus aqueous leaf extract intraperitoneally.Blood and serum samples were collected from each group and examined for various blood parameters.The turbidimetric assay for lysozyme activity using M.luteus and the survival rate of fish against A.hydrophila was carried out.Results:There was no significant impact(P>0.05)on white blood cell count,red blood cell count,mean corpuscular volume,mean corpuscular hemoglobin,mean corpuscular hemoglobin concentration and lysozyme activity was significantly(P<0.05)higher than control.The serum glutamic oxaloacetic transaminase and serum glutamic pyruvic transaminase levels were significantly lower than control(P<0.05).The highest dose group showed longest survival.Conclusions:From this study,it is considered that the aqueous extract of P.minus can be used as an immunostimulant in African catfish,but continuous administration may require maintaining the protection.
