CYP450 plays an essential role in the development and growth of the fruits of Siraitia grosvenorii. However, little is known about the SgCYP450-4 gene in S. grosvenorii. Here, based on transcriptome data, a full-lengt...CYP450 plays an essential role in the development and growth of the fruits of Siraitia grosvenorii. However, little is known about the SgCYP450-4 gene in S. grosvenorii. Here, based on transcriptome data, a full-length c DNA sequence of SgCYP450-4 was cloned by reverse transcriptasepolymerase chain reaction(RT-PCR) and rapid-amplification of c DNA ends(RACE) strategies. SgCYP450-4 is 1677 bp in length(Gen Bank accession No. AEM42985.1) and contains a complete open reading frame(ORF) of 1422 bp. The deduced protein was composed of 473 amino acids, the molecular weight is 54.01 kDa, the theoretical isoelectric point(PI) is 8.8, and the protein was predicted to possess cytochrome P450 domains. SgCYP450-4 gene was highly expressed in root, diploid fruit and fruit treated with hormone and pollination. At 10 days after treatment with pollination and hormones, the expression of SgCYP450-4had the highest level and then decreased over time, which was consistent with the development of fruits of S.Grosvenorii. Hormonal treatment could significantly induce the expression of SgCYP450-4. These results provide a reference for regulation of fruit development and the use of parthenocarpy to generate seedless fruit, and provide a scientific basis for the production of growth regulator application agents.展开更多
High-thermally conductive AlN-based microwave attenuating composite ceramics with spherical graphite(SG) as the attenuating agent were fabricated through hot-pressing sintering. The SG maintains its three-dimensional(...High-thermally conductive AlN-based microwave attenuating composite ceramics with spherical graphite(SG) as the attenuating agent were fabricated through hot-pressing sintering. The SG maintains its three-dimensional(3D) morphology within the sintered bodies, which considerably impedes the sintering of the composites to some extent but slightly influences on the growth of AlN grains. The addition of SG reduces the strength of the composites, but provides a moderate toughening effect at the optimal addition amount(3.8 MPa·m^(1/2) at 4 wt% SG). Benefiting from the low anisotropy, high thermal conductivity, and the 3D morphology of SG, the composites exhibit a relatively higher thermal conductivity(76.82 W·m^(–1)·K^(–1) at 10 wt% SG) compared with composites added with non-spherical attenuating agent. The dielectric constant and loss(8.2–12.4 GHz) increase remarkably as the amount of SG added increases up to 8 wt%, revealing that the incorporation of SG improves the dielectric property of the composite. The composite with 7 wt% SG exhibits the best absorption performance with a minimum reflection loss of –13.9 dB at 12.4 GHz and an effective absorbing bandwidth of 0.87 GHz. The excellent overall properties of the SG/AlN microwave attenuating composites render them as a promising material for various applications. Moreover, SG has a great potential as an attenuating agent for microwave attenuating composites due to its strong attenuation upon integration, high thermal conductivity, and low anisotropy.展开更多
基金supported by grants from the National Natural Science Foundation of China(Nos.81373914,81573521 and31400275)Program for Innovation Research Team(No.IT1306)of Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College
文摘CYP450 plays an essential role in the development and growth of the fruits of Siraitia grosvenorii. However, little is known about the SgCYP450-4 gene in S. grosvenorii. Here, based on transcriptome data, a full-length c DNA sequence of SgCYP450-4 was cloned by reverse transcriptasepolymerase chain reaction(RT-PCR) and rapid-amplification of c DNA ends(RACE) strategies. SgCYP450-4 is 1677 bp in length(Gen Bank accession No. AEM42985.1) and contains a complete open reading frame(ORF) of 1422 bp. The deduced protein was composed of 473 amino acids, the molecular weight is 54.01 kDa, the theoretical isoelectric point(PI) is 8.8, and the protein was predicted to possess cytochrome P450 domains. SgCYP450-4 gene was highly expressed in root, diploid fruit and fruit treated with hormone and pollination. At 10 days after treatment with pollination and hormones, the expression of SgCYP450-4had the highest level and then decreased over time, which was consistent with the development of fruits of S.Grosvenorii. Hormonal treatment could significantly induce the expression of SgCYP450-4. These results provide a reference for regulation of fruit development and the use of parthenocarpy to generate seedless fruit, and provide a scientific basis for the production of growth regulator application agents.
基金financially supported by the Priority Academic Program Development of Jiangsu Higher Education Institutionsthe Postgraduate Research & Practice Innovation Program of Jiangsu Province (KYCX200990)+2 种基金Qing Lan Projectthe Program for Changjiang Scholars, Innovative Research Team in University (IRT1146 and IRT15R35)the Top-notch Academic Programs Project of Jiangsu Higher Education Institutions (TAPP, PPZY2015B128)。
文摘High-thermally conductive AlN-based microwave attenuating composite ceramics with spherical graphite(SG) as the attenuating agent were fabricated through hot-pressing sintering. The SG maintains its three-dimensional(3D) morphology within the sintered bodies, which considerably impedes the sintering of the composites to some extent but slightly influences on the growth of AlN grains. The addition of SG reduces the strength of the composites, but provides a moderate toughening effect at the optimal addition amount(3.8 MPa·m^(1/2) at 4 wt% SG). Benefiting from the low anisotropy, high thermal conductivity, and the 3D morphology of SG, the composites exhibit a relatively higher thermal conductivity(76.82 W·m^(–1)·K^(–1) at 10 wt% SG) compared with composites added with non-spherical attenuating agent. The dielectric constant and loss(8.2–12.4 GHz) increase remarkably as the amount of SG added increases up to 8 wt%, revealing that the incorporation of SG improves the dielectric property of the composite. The composite with 7 wt% SG exhibits the best absorption performance with a minimum reflection loss of –13.9 dB at 12.4 GHz and an effective absorbing bandwidth of 0.87 GHz. The excellent overall properties of the SG/AlN microwave attenuating composites render them as a promising material for various applications. Moreover, SG has a great potential as an attenuating agent for microwave attenuating composites due to its strong attenuation upon integration, high thermal conductivity, and low anisotropy.