In order to provide an objective and scientific theoretical basis for rational distribution of wheat growth in Yunnan Province,according to the relationship between Yunnan weather conditions and wheat growth adaptabil...In order to provide an objective and scientific theoretical basis for rational distribution of wheat growth in Yunnan Province,according to the relationship between Yunnan weather conditions and wheat growth adaptability,a study on eco-climate type regionalization of wheat growing areas in Yunnan was conducted using principal component analysis and GIS technology. The results show that Yunnan Province could be divided into four types,namely southern warm and humid wheat growing area,central semi-arid wheat growing area,central semi-humid wheat growing area and north-central cold wheat growing area.展开更多
High-order nonlinear multiphoton absorption is usually inefficient,but can be enhanced by designing resonant excitations between occupied and unoccupied energy levels.We conducted angle-resolved multi-photon photoemis...High-order nonlinear multiphoton absorption is usually inefficient,but can be enhanced by designing resonant excitations between occupied and unoccupied energy levels.We conducted angle-resolved multi-photon photoemission(mPPE)studies on the SnSe_(2)(001)surfaces excited by ultrashort laser pulses.By tuning photon energy and light polarization,we demonstrate the presence of a resonant four-photon photoemission(4PPE)process involving the occupied valence band(VB),the unoccupied second conduction band(CB2)and the unoccupied image-potential state(IPs)of SnSe_(2).In this 4PPE process,VB electrons of SnSe_(2) are resonantly excited into CB2 by adsorbing two photons,followed by the adsorption of another photon to populate the n=1 IPs before being emitted out to the vacuum by adsorbing one more photon.This results in a double-resonant 4PPE process,which exhibits approximately a 40 times enhancement in photoemission yields compared to cases where one of the resonant pathways,CB2→IPs,is inhibited by involving a virtual state instead of the IPs in the 4PPE.The double-resonant 4PPE process efficiently excite the bulk VB electrons outside the vacuum,like taking advantage of resonant“ladders”through two real empty electronic states of SnSe_(2).Our results highlight the important applications of mPPE in probing the band-structure,particularly the unoccupied states,of recently emerging main group dichalcogenide semiconductors.Furthermore,the discovered resonant mPPE process contributes to the exploration of their promising optoelectronic applications.展开更多
The quantum harmonic oscillator(QHO),one of the most important and ubiquitous model systems in quantum mechanics,features equally spaced energy levels or eigenstates.Here we present a new class of nearly ideal QHOs fo...The quantum harmonic oscillator(QHO),one of the most important and ubiquitous model systems in quantum mechanics,features equally spaced energy levels or eigenstates.Here we present a new class of nearly ideal QHOs formed by hydrogenic substitutional dopants in an AlGaAs/GaAs heterostructure.On the basis of model calculations,we demonstrate that,when aδ-doping Si donor substitutes the Ga/Al lattice site close to AlGaAs/GaAs heterointerface,a hydrogenic Si QHO,characterized by a restoring Coulomb force producing square law harmonic potential,is formed.This gives rise to QHO states with energy spacing of~8–9 meV.We experimentally confirm this proposal by utilizing gate tuning and measuring QHO states using an aluminum single-electron transistor(SET).A sharp and fast oscillation with period of~7–8 mV appears in addition to the regular Coulomb blockade(CB)oscillation with much larger period,for positive gate biases above 0.5 V.The observation of fast oscillation and its behavior is quantitatively consistent with our theoretical result,manifesting the harmonic motion of electrons from the QHO.Our results might establish a general principle to design,construct and manipulate QHOs in semiconductor heterostructures,opening future possibilities for their quantum applications.展开更多
Promoters are critical elements to control gene expression but could behave differently under various growth conditions. Here we report the construction of a genome-wide promoter library, in which each native promoter...Promoters are critical elements to control gene expression but could behave differently under various growth conditions. Here we report the construction of a genome-wide promoter library, in which each native promoter in Saccharomyces cerevisiae was cloned upstream of a yellow fluorescent protein （YFP） reporter gene. Nine libraries were arbitrarily defined and assembled in bacteria. The resulting pools of promoters could be prepared and transformed into a yeast strain either as centromeric plasmids or integrated into a genomic locus upon enzymatic treatment. Using fluorescence activated cell sorting, we classified the yeast strains based on YFP fluorescence intensity and arbitrarily divided the entire library into 12 bins, representing weak to strong promoters. Several strong promoters were identified from the most active bins and their activities were assayed under different growth conditions. Finally, these promoters were applied to drive the expression of genes in xylose utilization to improve fermentation efficiency. Together, this library could provide a quick solution to identify and utilize desired promoters under user-defined growth conditions.展开更多
基金Supported by the National Special Founds for the Construction of Modern Agricultural Industrial Technology System (MATS)(CARS-3-2-45)Founds for Selection and Promotion of New High-quality Beer-feed Barley in Yunnan
文摘In order to provide an objective and scientific theoretical basis for rational distribution of wheat growth in Yunnan Province,according to the relationship between Yunnan weather conditions and wheat growth adaptability,a study on eco-climate type regionalization of wheat growing areas in Yunnan was conducted using principal component analysis and GIS technology. The results show that Yunnan Province could be divided into four types,namely southern warm and humid wheat growing area,central semi-arid wheat growing area,central semi-humid wheat growing area and north-central cold wheat growing area.
基金the Strategic Priority Research Program of the Chinese Academy of Sciences(Grant No.XD30000000)the National Key R&D Program of China(Grant Nos.2018YFA0305802 and 2017YFA0303500)the National Natural Science Foundation of China(Grant No.11774267).Calculations were performed at the supercomputing center of WHU of China.
文摘High-order nonlinear multiphoton absorption is usually inefficient,but can be enhanced by designing resonant excitations between occupied and unoccupied energy levels.We conducted angle-resolved multi-photon photoemission(mPPE)studies on the SnSe_(2)(001)surfaces excited by ultrashort laser pulses.By tuning photon energy and light polarization,we demonstrate the presence of a resonant four-photon photoemission(4PPE)process involving the occupied valence band(VB),the unoccupied second conduction band(CB2)and the unoccupied image-potential state(IPs)of SnSe_(2).In this 4PPE process,VB electrons of SnSe_(2) are resonantly excited into CB2 by adsorbing two photons,followed by the adsorption of another photon to populate the n=1 IPs before being emitted out to the vacuum by adsorbing one more photon.This results in a double-resonant 4PPE process,which exhibits approximately a 40 times enhancement in photoemission yields compared to cases where one of the resonant pathways,CB2→IPs,is inhibited by involving a virtual state instead of the IPs in the 4PPE.The double-resonant 4PPE process efficiently excite the bulk VB electrons outside the vacuum,like taking advantage of resonant“ladders”through two real empty electronic states of SnSe_(2).Our results highlight the important applications of mPPE in probing the band-structure,particularly the unoccupied states,of recently emerging main group dichalcogenide semiconductors.Furthermore,the discovered resonant mPPE process contributes to the exploration of their promising optoelectronic applications.
基金Profs.Y.Zhang,J.Chen,J.Zhao,and C.Lin are greatly appreciated.M.Feng thanks financial support from the National Key R&D Program of China(Grant No.2018YFA0305802)the Strategic Priority Research Program of Chinese Academy of Sciences(Grant No.XD30000000)+1 种基金the National Natural Science Foundation of China(Grant Nos.11574364 and 11774267)L.Mao thanks financial support from the National Key R&D Program of China by the Ministry of Science and Technology of China(Grant No.2015C8932400).
文摘The quantum harmonic oscillator(QHO),one of the most important and ubiquitous model systems in quantum mechanics,features equally spaced energy levels or eigenstates.Here we present a new class of nearly ideal QHOs formed by hydrogenic substitutional dopants in an AlGaAs/GaAs heterostructure.On the basis of model calculations,we demonstrate that,when aδ-doping Si donor substitutes the Ga/Al lattice site close to AlGaAs/GaAs heterointerface,a hydrogenic Si QHO,characterized by a restoring Coulomb force producing square law harmonic potential,is formed.This gives rise to QHO states with energy spacing of~8–9 meV.We experimentally confirm this proposal by utilizing gate tuning and measuring QHO states using an aluminum single-electron transistor(SET).A sharp and fast oscillation with period of~7–8 mV appears in addition to the regular Coulomb blockade(CB)oscillation with much larger period,for positive gate biases above 0.5 V.The observation of fast oscillation and its behavior is quantitatively consistent with our theoretical result,manifesting the harmonic motion of electrons from the QHO.Our results might establish a general principle to design,construct and manipulate QHOs in semiconductor heterostructures,opening future possibilities for their quantum applications.
基金financial support from the Ministry of Science and Technology(MOST)of China(2018YFE0202700,2017YFA0302903,and 2019YFA0308603)the National Natural Science Foundation of China(11622437,61674171,61761166009,11574392,11974421,11974422,11774422,12174443,and 12134020)+1 种基金the Strategic Priority Research Program of Chinese Academy of Sciences(XDB30000000)the Fundamental Research Funds for the Central Universities,and the Research Funds of Renmin University of China(22XNKJ30(W.J.)and 21XNH090(D.P.G.))。
基金We thank Jianhuo Fang at DNA sequencing facility in Tsinghua University for providing the sequencing service. This work was supported by the National Natural Science Foundation of China （Grant No. 31471254）, Chinese Ministry of Science and Technology grant 2012CB725201 and Tsinghua University Initiative grant 20161080088.
文摘Promoters are critical elements to control gene expression but could behave differently under various growth conditions. Here we report the construction of a genome-wide promoter library, in which each native promoter in Saccharomyces cerevisiae was cloned upstream of a yellow fluorescent protein （YFP） reporter gene. Nine libraries were arbitrarily defined and assembled in bacteria. The resulting pools of promoters could be prepared and transformed into a yeast strain either as centromeric plasmids or integrated into a genomic locus upon enzymatic treatment. Using fluorescence activated cell sorting, we classified the yeast strains based on YFP fluorescence intensity and arbitrarily divided the entire library into 12 bins, representing weak to strong promoters. Several strong promoters were identified from the most active bins and their activities were assayed under different growth conditions. Finally, these promoters were applied to drive the expression of genes in xylose utilization to improve fermentation efficiency. Together, this library could provide a quick solution to identify and utilize desired promoters under user-defined growth conditions.
