Lipocalin-2在肺癌患者血清中的表达及其临床意义

背景与目的肺癌是全球发病率最高的癌症类型,严重威胁着人类健康。肺癌的早期诊断、早期治疗对于肺癌患者的生存尤为重要。血清中的肿瘤标志物作为肿瘤早期诊断的一种重要方法已被广泛应用。然而,肺癌的早期诊断标志物还很少。本研究旨在探讨Lipocalin-2在肺癌患者血清中的表达水平及其临床意义。方法采用酶联免疫吸附法(enzyme linked immunosorbent assay,ELISA)检测Lipocalin-2在60例肺癌患者与63例健康人群外周血血清中的浓度,并分析Lipocalin-2表达水平与肺癌临床特征之间的关系。结果Lipocalin-2在肺癌患者外周血血清中的表达水平明显高于健康人群,差异具有明显统计学意义(P<0.001)。Lipocalin-2在肺癌患者中的表达与病理组织的分化、分期及淋巴结转移相关,差异具有明显统计学意义(P<0.05)。Lipocalin-2在病理分化差的肺癌患者血清中的表达高于分化良好患者;在发生淋巴结转移的肺癌患者血清中的表达高于没有发生淋巴结转移患者;在临床Ⅲ期+Ⅳ期肺癌患者中的表达水平显著高于临床Ⅰ期+Ⅱ期患者;差异均具有统计学意义(P<0.05)。结论Lipocalin-2在肺癌患者血清水平中高表达,与病理组织的分化、分期及淋巴结转移相关,有望成为一种潜在的用于临床诊断的新型肺癌肿瘤标志物。

Herbicidal Activities of Eupatorium adenophorum Extracts by 3 Extraction Methods

[ Objective ] The paper was to screen the better extraction method for herbicidal active substances of Eupatorium adenophorum. [ Method ] Three com- mon extraction methods, Soxhlet extraction, ultrasonic extraction and conventional extraction method, were used to prepare E. adenophorum extracts, and the herblcidal activity of E. adenophorum extracts extracted by 3 methods was studied by seed germination method. [ Result] The extraction rate of Soxhlet extraction was the highest of （22.54 ± 0.48 ）%, and the herbicidal activities of E. adenophorum extracted by Soxhlet extraction was relatively stronger. [ Conclusion] Soxhlet extraction was the better extraction method for herbicidal active substances of E. adenophorum.

Method of Obtaining Magnetic Object Location Based on Geomagnetic Field Intensity

It is very important to determine the magnetic object location using the magnetic anomaly. This paper presents a method for determined the magnetic object location using the geomagnetic field intensity. The magnetic object center position can be obtained by the amplitude of the analytic signal( AS) of geomagnetic total intensity. The vertical distance can be obtained by the value of magnetic anomaly on center position. But the vertical distance from measuring plane to magnetic object is not relatively precious because of not eliminating the geomagnetic normal field in the calculation process. The method of modeling geomagnetic normal field in small space is proposed based on Taylor polynomial. The impact of geomagnetic variation field for the precious of model is analyzed. Through the measured data and the model data,total intensity magnetic anomaly on the measuring plane is obtained which is used for calculating the vertical distance of magnetic object by calculating model. The experimental tests have been conducted on open field on the campus of Harbin Engineering University. The results show that the calculated results and real values match perfectly using this method.

Improvement of Teaching Experimental Design of Pesticide Bioassays

For mistakes taken in pesticide bioassays, teaching experimental design is improved in the paper, so as to let students explore and analyze in teaching experiments to get a deeper understanding of theoretical knowledge, thereby effectively avoiding frequently-taken mistakes in pesticide bioassays.

Preliminary Study on Rooting Promotion Activity of 33 Species of Plant Extracts

[ Objective ] The paper was to study the rooting promotion activity of plant extracts, so as to lay the foundation for development of natural source of roo- fing reagent with high efficiency, safety and environment friendship. [Method] Using mung bean rooting method, the rooting promotion effects of 33 plant extracts on mung bean section were studied. [Result] When the concentration was 1 mg/mL, Eupatorium adenophorum （stem and leaf）, Cryptomeriafortunei （stem and leaf）, Pinus yunnanensis （stem）, Pinus yunnanensis （leaf）, Rhododendron simsii （leaf）, Pinus armandii （leaf）, Litchi chinensis （pericarp）, P/nus armandiifruit （fruit） and Alnus cremastogyne （leaf） showed strong promotion effect on rooting of mung bean sections, and the rooting promotion rate reached over 100% ; the roo- ting promotion effect ofE. adenophorum （stem and leaf） was the strongest, reaching（1068.42 ＋7.87）%, which was extremely higher than the extracts of other plants. [Conclusion] E. adenophorum （stem and leaf）, C. fortunei （stem and leaf）, P. yunnanens/s （stem）, P. yurmanens/s （leaf）, R. simsii （leaf）, P. armandii （leaf）, L. chinensis （pericarp）, P. armandiifruit （fruit） and A. cremastogyne （leaf） all contained substances with st/ong rooting promotion effect, which could be further used for development of natural rooting reagent.

Tb(Ⅲ)functionalized MOF based self-calibrating sensor integrated with logic gate operation for efficient epinephrine detection in serum

By anchoring Tb^(3+)ions on its free carboxyl groups of the nanocaged NiMOF,a dual-emission self-calibrating sensor of Tb^(3+)@NiMOF was fabricated through coordination post-synthetic modification(PSM)strategy.With Tb^(3+)ions as the secondary fluorescent signal and sensing active sites,Tb^(3+)@NiMOF presents great potentials in visually and efficiently monitoring EPI in serum,with high sensitivity and selectivity,fast response,excellent recyclable,and the low detection limit(LOD,3.06 ng/mL).Furthermore,a tandem combinational logic gate based intelligent detection system was constructed to improve the practicability and convenience of epinephrine(EPI)detection in serum by comparing the light emitted colour with the series standard colour cards preset in the smartphone.This work provides a promising approach of developing metal-organic frameworks(MOFs)based self-calibrating sensors for intelligent detection of bioactive molecules.

黄曲霉毒素B1降解菌的筛选及在玉米贮藏中的应用

【目的】本研究分离筛选出一株对黄曲霉既有抑制作用又能降解其毒素的拮抗细菌菌株,并将其应用于玉米中的黄曲霉污染防治研究。【方法】试验通过平板筛选法结合玉米活体筛选法对黄曲霉毒素B1 (AFB1)的拮抗细菌进行初筛,以AFB1的降解率和抑制率为指标进行复筛。【结果】分离到的菌株对黄曲霉菌的抑制率为79.20%,对1μg/mL的黄曲霉毒素B1的降解率为68.39%。贮藏期玉米含水量在15%–30%时,该菌株对黄曲霉污染的抑制率与玉米含水量成反比,即玉米含水量在15%时其抑制率达92.46%,玉米含水量在30%时其抑制率为19.41%。玉米含水量在28%时,菌株对黄曲霉污染玉米的防治效果达到36.39%。【结论】筛选出的拮抗菌株为枯草芽孢杆菌,该菌株不仅对黄曲霉菌有抑制作用,而且能减少AFB1对玉米的污染。

Robust fault-tolerant attitude control for satellite with multiple uncertainties and actuator faults

In this paper,the satellite attitude control system subject to parametric perturbations,external disturbances,time-varying input delays,actuator faults and saturation is studied.In order to make the controller architecture simple and practical,the closed-loop system is transformed into a disturbance-free nominal system and an equivalent disturbance firstly.The equivalent disturbance represents all above uncertainties and actuator failures of the original system.Then a robust controller is proposed in a simple composition consisting of a nominal controller and a robust compensator.The nominal controller is designed for the transformed nominal system.The robust compensator is developed from a second-order filter to restrict the influence of the equivalent disturbance.Stability analysis indicates that both attitude tracking errors and compensator states can converge into the given neighborhood of the origin in finite time.To verify the effectiveness of the proposed control law,numerical simulations are carried out in different cases.Presented results demonstrate that the high-precision attitude tracking control can be achieved by the proposed fault-tolerant control law.Furthermore,multiple system performances including the control accuracy and energy consumption index are fully discussed under a series of compensator parameters.

Controllable design of high-efficiency triboelectric materials by functionalized metal–organic frameworks with a large electronwithdrawing functional group

Triboelectric nanogenerator(TENG)can directly convert mechanical energy into electric energy.However,the triboelectric materials are limited to the triboelectric series.Here,for the first time,we choose the isostructural UiO-66-X(X=H,NH_(2),NO_(2),and Br)family as triboelectric materials to investigate the underlying relationships between different functional groups and the triboelectric performance of TENG.Unlike traditional triboelectric material organic polymers,metal–organic frameworks(MOFs)can be oriented design synthesis and functionalized with various functional groups.The results demonstrate that the largest output voltage and current are from UiO-66-NO_(2) TENG,and are about 23.79 V and 0.29μA,which are 3.19 and 4.14 times over that of the UiO-66 TENG,respectively.The working mechanism of the MOF TENG was discussed in depth through experiments and theoretical calculations.This work proves a novel strategy to obtain high output properties by functionalized MOFs with large electron-withdrawing functional groups and promising guidance for the choice of high-efficiency triboelectric materials.