Soluble sugar and organic acids are key determinants of fruit organoleptic quality and directly affect the commodity value and economic returns of fruit crops.We performed whole-genome sequencing of the apple varietie...Soluble sugar and organic acids are key determinants of fruit organoleptic quality and directly affect the commodity value and economic returns of fruit crops.We performed whole-genome sequencing of the apple varieties Gala and Xiahongrou,along with their F1 hybrids,to construct a high-density bin map.Our quantitative genetic analysis pinpointed 53 quantitative trait loci(QTLs)related to 11 sugar and acid traits.We identified a candidate gene,MdNADP-ME,responsible for malate degradation,in a stable QTL on linkage group 15.Sequence analysis revealed an A/C SNP in the promoter region(MEp-799)that influences binding of the MdMYB2 transcription factor,thereby affecting MdNADP-ME expression.In our study of various apple genotypes,this SNP has been demonstrated to be linked to malate and fructose levels.We also developed a dCAPS marker associated with fruit fructose content.These results substantiate the role of MdNADP-ME in maintaining the equilibrium between sugar and acid contents in apple fruits.展开更多
基金upported by the National Key Research and Development Program of China,“Physiological basis and regulation of fruit tree quality and high yield”(2019YFD1000103)a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD).
文摘Soluble sugar and organic acids are key determinants of fruit organoleptic quality and directly affect the commodity value and economic returns of fruit crops.We performed whole-genome sequencing of the apple varieties Gala and Xiahongrou,along with their F1 hybrids,to construct a high-density bin map.Our quantitative genetic analysis pinpointed 53 quantitative trait loci(QTLs)related to 11 sugar and acid traits.We identified a candidate gene,MdNADP-ME,responsible for malate degradation,in a stable QTL on linkage group 15.Sequence analysis revealed an A/C SNP in the promoter region(MEp-799)that influences binding of the MdMYB2 transcription factor,thereby affecting MdNADP-ME expression.In our study of various apple genotypes,this SNP has been demonstrated to be linked to malate and fructose levels.We also developed a dCAPS marker associated with fruit fructose content.These results substantiate the role of MdNADP-ME in maintaining the equilibrium between sugar and acid contents in apple fruits.
