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1株野生蝉虫草的鉴定及其胞内和胞外多糖抗肝癌活性比较 被引量:5
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作者 刘城移 郝心怡 +4 位作者 林沛霖 黄海辰 刘昆 吴小平 傅俊生 《西北农林科技大学学报(自然科学版)》 CSCD 北大核心 2020年第12期117-126,共10页
【目的】对1株野外采集的蝉虫草进行分离和鉴定,分析比较其发酵菌丝体胞内和胞外多糖的抗肝癌细胞HepG-2活性及多糖的组成。【方法】通过形态学特征和ITS序列分析,对供试菌株进行鉴定;采用MTT法、细胞划痕试验,比较供试菌株菌丝体胞内... 【目的】对1株野外采集的蝉虫草进行分离和鉴定,分析比较其发酵菌丝体胞内和胞外多糖的抗肝癌细胞HepG-2活性及多糖的组成。【方法】通过形态学特征和ITS序列分析,对供试菌株进行鉴定;采用MTT法、细胞划痕试验,比较供试菌株菌丝体胞内和胞外多糖对人肝癌细胞HepG-2的抑制活性以及对人正常肝细胞LO2的毒性,并通过高效液相色谱法和凝胶渗透色谱法,比较分析2种多糖的成分及分子质量分布。【结果】根据菌株形态学特征和ITS序列分析,供试菌株为蝉虫草Cordyceps cicadae;其胞内和胞外多糖均可极显著抑制HepG-2细胞活性(P<0.01),半数抑制浓度(IC50)值分别为3.23和0.45 mg/mL,细胞迁移率分别为38.72%和24.79%,提示胞外多糖比胞内多糖具有更强的抗肝癌细胞HepG-2活性;且2种多糖对人LO2细胞均无明显毒性;进一步分析发现,胞外多糖中半乳糖醛酸的含量是胞内多糖的23.53倍,其分子质量的94.1%集中在19.1~85.0 ku,胞内多糖中核糖是其特征性单糖,高分子质量多糖(>250.0 ku)分布占比明显高于胞外多糖。【结论】野生蝉虫草菌株发酵所产胞外多糖的抗肝癌细胞HepG-2活性较胞内多糖强,二者在单糖组成和分子质量分布上存在明显差异。 展开更多
关键词 蝉虫草 分离与鉴定 胞内胞外多糖 抗肝癌活性
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观赏南瓜新品种‘南珠11号’的选育
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作者 陈继敏 杨镇明 +3 位作者 黄云鲜 林沛林 任淑梅 沈汉国 《中国瓜菜》 CAS 北大核心 2019年第3期31-33,I0001,共4页
‘南珠11号’观赏南瓜是从台湾农友种苗股份有限公司‘瓜皮南瓜’经6代自交分离选育的优良自交系小果型观赏南瓜新品种。该品种生长势及分枝性强,主侧蔓结果,平均第1雌花节位20.9节,单株结果可达20个以上。果实扁球形,果形端正美观,果... ‘南珠11号’观赏南瓜是从台湾农友种苗股份有限公司‘瓜皮南瓜’经6代自交分离选育的优良自交系小果型观赏南瓜新品种。该品种生长势及分枝性强,主侧蔓结果,平均第1雌花节位20.9节,单株结果可达20个以上。果实扁球形,果形端正美观,果皮浅黄色,有条斑和瘤状突起。果实平均纵径6.1 cm、横径8.4 cm,平均单瓜质量95.0 g。观赏周期145 d左右。设施栽培条件下病毒病等病害极少发生。2017年1月通过广东省农作物品种审定委员会审定,适合广东地区秋季设施栽培。 展开更多
关键词 观赏南瓜 新品种 '南珠11号’
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Coronary heart disease: incidence, risk factors and interventions in Jiaozhou of Shandong province 被引量:5
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作者 Yu Hua Li Dan +10 位作者 Chu Xianming An Yi Song Tongxun Feng Huixin lin peilin Wang Tao Jiang Shaoyan Guo linlin Xu Fengqiang Liu Zhengke Yang Bin 《Chinese Medical Journal》 SCIE CAS CSCD 2014年第12期2275-2278,共4页
Background Coronary heart disease (CHD) is the most common type of heart disease and cause of heart attacks.This study investigated the epidemiological characteristics of CHD and its risk factors in Jiaozhou,Shandon... Background Coronary heart disease (CHD) is the most common type of heart disease and cause of heart attacks.This study investigated the epidemiological characteristics of CHD and its risk factors in Jiaozhou,Shandong province,to ultimately find a way of reducing the prevalence of cardiovascular disease,and to provide a theoretical basis for establishing a cardiovascular disease management path under the regional medical collaborative mechanism.Methods A questionnaire survey was performed including 1 952 people aged 35 years or older who were questioned by means of stratified,cluster,proportional sampling to investigate the prevalence of CHD and its risk factors.The data were inputted into SPSS11.0 statistical software for processing and analysis.We advised the local medical institutions to establish health files for the residents with CHD and risk factors.They were followed up regularly.Their risk factors and life-style were monitored,and advice was given as to proper medications.Green channels were established,and the patients were transmitted in a timely manner to superior hospitals for better treatment if the necessary treatments were not available in the local hospitals.The control of risk factors was observed after the follow-up for half a year.Results In Jiaozhou,the rates of coronary artery disease,hypertension,diabetes,hyperlipidemia and overweight were 8.15%,28.54%,11.43%,35.46%,and 18.70% respectively.The rates of hypertension,diabetes,hyperlipidemia and overweight were higher than the data published in "The report of Chinese cardiovascular disease 2012"; which are 24%,9.7%,18.6%,and 9.7%,respectively.The control of risk factors improved significantly after the guidance of the residents lifestyle and medication for six months.Conclusions The high prevalence of coronary artery disease in Jiaozhou is closely related to age,gender,diet structure,family history of cardiovascular disease,hypertension,diabetes,hyperlipidemia,overweight,and unhealthy lifestyle.Under the regional medical coordination mechanism,the collaborative management of cardiovascular disease can provide new management concepts for the areas short of medical resources,so as to reduce the prevalence of cardiovascular disease. 展开更多
关键词 coronary heart disease risk factors PREVALENCE health file regional medical collaboration
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