Helicobacter pylori infection may result in poor gastric cleanliness in magnetically controlled capsule gastroscopy examination: A singlecenter retrospective study

Objective:Magnetically controlled capsule gastroscopy(MCCG)is an effective method for screening gastric diseases;however,its performance may be affected by gastric cleanliness.We aimed to explore the correlation between Helicobacter pylori infection and the degree of gastric cleanliness in the MCCG.Methods:This retrospective study enrolled 297 participants from October 2020 to April 2024 at Sir Run Run Shaw Hospital,Zhejiang University School of Medicine.Participant characteristics,MCCG examination results,and(13)C-urea breath test(C13-UBT)results were collected.The gastric cleanliness in MCCG examinations was assessed using a gastric cleanliness score.Binary logistic regression was used to analyze the relationships among participant characteristics,H.pylori infection,and gastric cleanliness.Chi-square tests and Fisher's exact tests were used to analyze the relationships among gastric lesions,H.pylori infection,and gastric cleanliness.Results:Among the participants,24.2%had H.pylori infection,and 17.5%had poor gastric cleanliness.Hypertension(odds ratio[OR]:2.63;95%confidence interval[CI]:1.36e5.09;p?0.004)was associated with a greater likelihood of H.pylori infection.H.pylori infection(OR:3.76;95%CI:1.99e7.09;p<0.001)was an independent risk factor for poor gastric cleanliness in the MCCG.A significant disparity was noted in the prevalence of focal erosions(p<0.001),gastric ulcers(p?0.001),and positive gastric lesions(p?0.027)between the 2 groups with and without H.pylori infection.The proportion of positive gastric lesions was not significantly different between the good gastric cleanliness group and the poor gastric cleanliness group(25.7%vs.21.2%;p?0.490).Conclusion:The findings of this study revealed that H.pylori infection was associated with hypertension.H.pylori infection may lead to poor gastric cleanliness.Institutions are advised to perform C13-UBT before MCCG,and participants should be informed of the risk of poor gastric cleanliness if the results are positive.The decision to perform H.pylori eradication before MCCG should take into account patient willingness and the benefit-to-risk ratio.

In situ atomic-scale tracking of unusual interface reaction circulation and phase reversibility in(de)potassiated alloy-typed anode

Alloy-typed anode materials,endowed innately with high theoretical specific capacity,hold great promise as an alternative to intercalation-typed counterparts for alkali-ion batteries.Despite tremendous efforts devoted to addressing drastic volume change and severe pulverization issues of such anodes,the underlying mechanisms involving dynamic phase evolutions and reaction kinetics have not yet been fully comprehended.Herein,taking antimony(Sb)anode as a representative paradigm,its microscopic operating mechanisms down to the atomic scale during live(de)potassiation cycling are systematically unraveled using in situ transmission electron microscopy.Highly reversible phase transformations at single-particle level,that are Sb←→KSb_(2)←→KSb←→K_5Sb_(4)←→K_(3)Sb,were revealed during cycling.Meanwhile,multiple phase interfaces associated with different reaction kinetics coexisted and this phenomenon was properly elucidated in the context of density functional theory calculations.Impressively,previously unexplored unidirectional circulation of reaction interfaces within individual Sb particle is confirmed for both potassiation and depotassiation.Based on the empirical results,the surface diffusion-mediated potassiation-depotassiation pathways at single-particle level are suggested.This work affords new insights into energy storage mechanisms of Sb anode and valuable guidance for targeted optimization of alloy-typed anodes(not limited to Sb)toward advanced potassium-ion batteries.

RcbHLH59-RcPRs module enhances salinity stress tolerance by balancing Na^(+)/K^(+) through callose deposition in rose (Rosa chinensis)

Basic helix–loop–helix(bHLH)proteins play pivotal roles in plant growth,development,and stress responses.However,the molecular and functional properties of bHLHs have not been fully characterized.In this study,a novel XI subgroup of the bHLH protein gene RcbHLH59 was isolated and identified in rose(Rosa sp.).This gene was induced by salinity stress in both rose leaves and roots,and functioned as a transactivator.Accordingly,silencing RcbHLH59 affected the antioxidant system,^(Na+/K+)balance,and photosynthetic system,thereby reducing salt tolerance,while the transient overexpression of RcbHLH59 improved salinity stress tolerance.Additionally,RcbLHLH59 was found to regulate the expression of sets of pathogenesis-related(PR)genes in RcbHLH59-silenced(TRV-RcbHLH59)and RcbHLH59-overexpressing(RcbHLH59-OE)rose plants.The RcPR4/1 and RcPR5/1 transcript levels showed opposite changes in the TRVRcbHLH59 and RcbHLH59-OE lines,suggesting that these two genes are regulated by RcbHLH59.Further analysis revealed that RcbHLH59 binds to the promoters of RcPR4/1 and RcPR5/1,and that the silencing of RcPR4/1 or RcPR5/1 led to decreased tolerance to salinity stress.Moreover,callose degradation-and deposition-related genes were impaired in RcPR4/1-or RcPR5/1-silenced plants,which displayed a salt tolerance phenotype by balancing the ^(Na+/K+)ratio through callose deposition.Collectively,our data highlight a new RcbLHLH59-RcPRs module that positively regulates salinity stress tolerance by balancing Na^(+)/K^(+)and through callose deposition in rose plants.

黑龙江省扶余市麦穗鱼舌状绦虫裂头蚴的分子鉴定和系统发育分析

为鉴定从黑龙江省扶余市麦穗鱼腹腔内收集的绦虫裂头蚴的种类,本试验采用PCR方法分别对其核糖体内转录间隔区(ITS)和线粒体细胞色素C氧化酶亚基Ⅰ(COⅠ)基因进行扩增和序列测定。运用DNASTAR软件的MegAlign程序分析核苷酸同源性,采用邻接法构建基于ITS和COⅠ基因序列的系统进化树,以此分析分离虫株种内与种间的系统发育关系。结果显示,各分离虫株的ITS1和ITS2基因与舌状绦虫(AY121751)的核苷酸序列同源性最高,分别为98.8%~100%和99.6%~99.7%;各分离虫株的COⅠ基因与肠舌状绦虫(EU241273)的核苷酸序列同源性最高,为91.2%~94.7%。基于ITS序列构建的系统进化树显示,舌状绦虫和双线绦虫的ITS序列具有高度的保守性,ITS区域可能不适宜作为区分舌状绦虫属内部种类的可靠分子标记。基于COⅠ基因构建的系统进化树显示,分离虫株与土耳其、俄罗斯和欧洲的肠舌状绦虫分离株进化关系最近。根据序列同源性和COⅠ基因进化树结果,初步将本试验所分离虫株鉴定为肠舌状绦虫。结果表明,COⅠ基因更适合于舌状绦虫属种类的鉴别。本试验为舌状绦虫的分子流行病学调查提供了参考。

基于CRISPR/Cas系统的生物传感器在动物疫病诊断中的应用

动物疫病不仅严重危害动物健康,阻碍养殖业良性发展,而且对人类健康构成潜在威胁。开发灵敏、特异、快速的诊断方法对于疫病防控尤为重要。PCR是目前检测病原体核酸的金标准,但是它存在检测周期较长,需要精密仪器和专业技术人员,无法应用于现场即时检测等局限性。成簇规律间隔短回文重复序列及其相关蛋白(clustered regularly interspaced short palindromic repeats/CRISPR-associated protein,CRISPR/Cas)具有敏感性高、特异性强和操作简便等优点,可为动物疫病诊断提供新的方法和契机。CRISPR-Cas9靶向核酸的特异性和CRISPR-Cas12/Cas13“附属切割”活性使其在核酸检测中显示出巨大的应用前景。本文简述了CRISPR/Cas系统的分类,对3种常用的CRISPR/Cas系统的核酸检测策略进行系统阐述,并对其在动物疫病诊断中应用的最新研究进展进行综述,最后讨论了它的优势、面临的挑战及未来发展方向,以期为动物疫病诊断新方法的开发提供参考。

Exogenous calcium enhances the physiological status and photosynthetic capacity of rose under drought stress

Drought(water shortage)can substantially limit the yield and economic value of rose plants(Rosa spp.).Here,we characterized the effect of exogenous calcium(Ca^(2+))on the antioxidant system and photosynthesis-related properties of rose under polyethylene glycol 6000(PEG6000)-induced drought stress.Chlorophyll levels,as well as leaf and root biomass,were significantly reduced by drought;drought also had a major effect on the enzymatic antioxidant system and increased concentrations of reactive oxygen species.Application of exogenous Ca^(2+)increased the net photosynthetic rate and stomatal conductance of leaves,enhanced water-use efficiency,and increased the length and width of stomata following exposure to drought.Organ-specific physiological responses were observed under different concentrations of Ca^(2+).Application of 5 mmol·L^(-1)Ca^(2+)promoted photosynthesis and antioxidant activity in the leaves,and application of 10 mmol·L^(-1)Ca^(2+)promoted antioxidant activity in the roots.Application of exogenous Ca^(2+)greatly enhanced the phenotype and photosynthetic capacity of potted rose plants following exposure to drought stress.Overall,our findings indicate that the application of exogenous Ca^(2+)enhances the drought resistance of roses by promoting physiological adaptation and that it could be used to aid the cultivation of rose plants.

Optimization of the OCO-2 Cloud Screening Algorithm and Evaluation against MODIS and TCCON Measurements over Land Surfaces in Europe and Japan

A method to tighten the cloud screening thresholds based on local conditions is used to provide more stringent schemes for Orbiting Carbon Observatory-2(OCO-2)cloud screening algorithms.Cloud screening strategies are essential to remove scenes with significant cloud and/or aerosol contamination from OCO-2 observations,which helps to save on the data processing cost and ensure high quality retrievals of the column-averaged CO2 dry air mole fraction(XCO2).Based on the radiance measurements in the 0.76μm O2A band,1.61μm(weak),and 2.06μm(strong)CO2 bands,the current combination of the A-Band Preprocessor(ABP)algorithm and Iterative Maximum A Posteriori(IMAP)Differential Optical Absorption Spectroscopy(DOAS)Preprocessor(IDP)algorithm passes around 20%-25%of all soundings,which means that some contaminated scenes also pass the screening process.In this work,three independent pairs of threshold parameters used in the ABP and IDP algorithms are sufficiently tuned until the overall pass rate is close to the monthly clear-sky fraction from the MODIS cloud mask.The tightened thresholds are applied to observations over land surfaces in Europe and Japan in 2016.The results show improvement of agreement and positive predictive value compared to the collocated MODIS cloud mask,especially in summer and fall.In addition,analysis indicates that XCO2 retrievals with more stringent thresholds are in closer agreement with measurements from collocated Total Carbon Column Observing Network(TCCON)sites.

生物钟相关基因在原发性肝细胞癌中的表达及临床意义——基于TCGA数据集分析

目的:探究生物钟相关基因在原发性肝细胞癌(简称肝癌)中的表达及意义。方法:从美国癌症和肿瘤基因图谱数据库(The Cancer Genome Atlas,TCGA)中下载肝癌数据424例,包括374例肝癌患者样本及50例正常对照样本。运用DECenter软件对51个生物钟相关基因进行差异表达分析,比较生物钟基因在肝癌患者及正常样本之间的表达情况。运用DAVID在线工具分析差异基因的GO及KEGG功能富集通路,并通过STRING数据库构建差异基因之间蛋白质相互作用网络。了解生物钟基因肝癌发生发展过程中的主要作用。结果:在374例肝癌患者样本的51个生物钟相关基因中,有21个基因在肝癌样本中表达上调(P<0.05),3个基因表达下调(P<0.05)。生物钟基因高表达样本主要富集于Wnt信号通路、Hedgehog信号通路及Hippo信号通路(P<0.001)。参与调节昼夜节律和细胞代谢的10个生物钟基因在蛋白质相互作用网络中相关性最为紧密(P<0.001)。GO分析差异基因集中作用于调节昼夜节律、细胞代谢过程、基因表达等通路(P<0.001)。结论:生物钟相关基因通过调节人体昼夜节律和细胞代谢参与原发性肝细胞癌的发生发展。

尿毒清颗粒联合甘精胰岛素治疗糖尿病肾病的效果及对氧化应激指标的影响

目的 探讨在糖尿病肾病的临床治疗中甘精胰岛素和尿毒清颗粒是否能够发挥药物协同作用。方法选取2021年6月-2022年12月福州经济技术开发区医院肾脏内分泌科收治的80例糖尿病肾病患者作为研究对象,随机分为观察组和对照组,每组40例。对照组采用甘精胰岛素联合前列地尔注射液治疗,观察组则采用甘精胰岛素联合尿毒清颗粒治疗,比较两组疗效等相关指标以及不良反应的发生率。结果 观察组总有效率为95.00%,高于对照组的77.50%,差异有统计学意义(P<0.05)。治疗后,观察组超氧化物歧化酶、谷胱甘肽过氧化物酶高于对照组,丙二醛低于对照组,差异有统计学意义(P<0.05)。治疗后,观察组空腹血糖、餐后2 h血糖均低于对照组,但差异无统计学意义(P>0.05)。治疗后,观察组肌酐、尿素氮及24 h尿蛋白均低于对照组,差异有统计学意义(P<0.05)。发生观察组不良反应发生率为12.50%,高于对照组的7.50%,但差异无统计学意义(P>0.05)。结论 在糖尿病肾病的治疗中甘精胰岛素是使用率比较高的一种药物,且效果良好,配合使用尿毒清颗粒能够发挥药物协同效果,取得更好的治疗效果。

肩峰下撞击综合征超声引导下介入治疗后复发危险因素

目的探讨肩峰下撞击综合征(SIS)2型超声介入治疗后复发的危险因素。方法回顾分析71例临床诊断SIS2型,根据治疗方案分为单纯注射组(超声引导下肩峰下滑囊注射,n=16)及联合治疗组(超声引导下肩袖肌腱松解术+肩峰下滑囊注射,n=55),采用Logistic多因素回归分析影响治疗后复发的因素。结果联合治疗组治疗后1、3、6个月复发率明显低于单纯注射组(P均<0.001)。多因素回归分析显示,年龄、性别、SIS2型病程均与复发无关,而治疗方法是治疗后复发的独立危险因素。结论采用超声引导下肩袖肌腱松解术+肩峰下滑囊注射治疗SIS2型患者可降低治疗后复发风险。

超声介入治疗肩峰下撞击综合征疗效对比分析

目的研究超声介入治疗肩峰下撞击综合征(SIS)患者的疗效及复发危险因素。方法回顾性分析92例SIS患者的临床资料,根据其超声表现进行分型,并分别采用超声引导肩峰下滑囊注射(单纯注射组)以及超声引导肩袖肌腱松解术联合肩峰下滑囊注射(联合治疗组),比较两组术后1周及1,3,6月的疗效差别。结果两组患者术后1周及1,3,6月的VAS,CMS和OSS评分较术前均有不同程度改善,但术后6月均有复发病例,单纯注射组及联合治疗组的晚期复发率分别为34.9%及10.2%,两组差别有统计学意义(P<0.05)。结论对于SIS的患者,采用超声引导下介入治疗有确切的临床疗效;但对于超声诊断为SIS 2型的患者,应采用超声引导肩袖肌腱松解术联合肩峰下滑囊注射,以降低晚期复发风险。

玉屏风散对变应性鼻炎模型大鼠TLR4/NF-κB信号通路的影响

目的观察玉屏风散对变应性鼻炎(AR)鼻黏膜上皮细胞TLR4/NF-κB信号通路的影响,探讨其作用机制。方法采用卵清白蛋白致敏建立AR大鼠模型。实验大鼠随机分为正常组、模型组和玉屏风散低、中、高剂量组。采用AR行为学指标进行评分,HE染色观察大鼠鼻黏膜形态;ELISA测定大鼠血清白细胞介素(IL)-4、IL-5、IL-10、免疫球蛋白E(IgE)和干扰素-γ(IFN-γ)含量,Western blot、RT-PCR检测大鼠鼻黏膜Toll样受体4(TLR4)和核因子(NF)-κBp65蛋白和基因的表达。结果与正常组比较,模型组大鼠血清IL-4、IL-5、IgE含量显著升高,IL-10和IFN-γ含量显著降低(P<0.01),鼻黏膜TLR4、NF-κBp65蛋白和基因表达显著升高(P<0.01);与模型组比较,玉屏风散中、高剂量组大鼠血清IL-4、IL-5、IgE含量显著降低,IL-10和IFN-γ含量显著升高(P<0.05,P<0.01),鼻黏膜TLR4、NF-κBp65蛋白和基因表达显著降低(P<0.05,P<0.01)。结论玉屏风散可能通过调节模型大鼠TLR4/NF-κB信号通路及相关炎性因子,而起到治疗AR的作用。

应用PCR-RFLP技术检测短喙矮小综合征鹅细小病毒

为准确诊断鸭短喙矮小综合征(SBDS)以及鉴别鹅细小病毒感染鸭群中新发短喙矮小综合征鹅细小病毒(SBDS-GPV),本研究通过对GenBank登录的水禽细小病毒基因组核苷酸序列的比对分析,根据该病毒5'非编码区基因序列特征设计一对特异性引物,建立了SBDS-GPV聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)检测方法。特异性试验和敏感性试验显示,水禽细小病毒特异性引物可以扩增得到约200 bp的目的片段,SBDS-GPV PCR产物能够被SspⅠ酶切为140 bp和60 bp两个片段,番鸭细小病毒与德兹西氏病小鹅瘟病毒则不能被酶切,检出SBDS-GPV DNA的最低浓度为2.28×10~7拷贝/μL。利用该方法对14份疑似SBDS临床样品检测,阳性样本采用SPF鸭胚进行病毒分离,结果显示SBDS-GPV PCR-RFLP检测的阳性样本经病毒分离均为SBDS-GPV。上述研究表明,该SBDS-GPV PCR-RFLP检测方法特异性强、敏感性高,为SBDS的防控提供了有效手段。

血清高病毒载量和乙型肝炎发作孕妇的抗病毒治疗

乙型肝炎病毒(hepatitis B virus,HBV)感染是中国重大公共卫生问题。2014年全国流行病学调查结果显示,0~4岁、4~15岁、15~29岁人群HBV感染率分别为0.32%、0.94%和4.38%,较2006年的调查结果明显下降,但育龄女性HBV感染率仍然在6%~8%[1]。另一方面,随着国家二胎生育政策的放开,如何正确处理HBV感染的育龄女性以及如何进一步减少HBV母婴垂直传播的问题仍是我们必须面对的重大课题。

胆管微错构瘤超声表现

胆管微错构瘤又称冯迈恩堡复合体(von Meyenburg complexe,VMC),临床少见,为肝内边缘胆管板畸形,最早由von Meyenbur于1918年报道。随着影像学技术的发展,对于该病有了较深入的认识[1]。本文报道4例VMC的超声表现。1资料与方法1.1研究对象回顾性分析2016年2月—2017年7月于福建医科大学附属第一医院经病理学诊断为VMC的4例患者,男2例,女2例;均无明显临床症状;分别来自2个家族,其中家族A的女性患者及家族B的男性患者体检时经超声发现肝实质弥漫性病变,随后排查2个家族其他成员共10名,确诊2例。

穴位贴敷联合西医常规治疗痰浊阻肺型慢性阻塞性肺疾病急性加重30例

目的:观察穴位贴敷联合西医常规治疗治疗痰浊阻肺型慢性阻塞性肺疾病急性加重(AECOPD)的临床效果。方法:选取2018年1月-2019年12月在笔者科室住院的60例痰浊阻肺型AECOPD患者,随机分为对照组30例和治疗组30例;对照组采用西医常规治疗,治疗组在西医常规治疗的基础上联合穴位贴敷,疗程为10 d,比较两组临床疗效、治疗前后第1秒用力呼气量(FEV1),观察穴位贴敷不良反应。结果:治疗组总有效率高于对照组,差异有统计学意义(P<0.05);治疗前两组FEV1比较差异无统计学意义(P>0.05),治疗后治疗组FEV1高于对照组,差异有统计学意义(P<0.05);治疗期间部分患者在贴敷后出现皮疹、红肿,伴瘙痒感、灼痛感,1周后自行痊愈。结论:穴位贴敷联合西医常规治疗可明显改善痰浊阻肺型AECOPD患者临床症状,提高整体疗效。

急救护理对急诊糖尿病酮症酸中毒患者的临床疗效及有效率分析

目的分析探究急诊糖尿病酮症酸中毒患者接受急救护理的临床疗效。方法2020年1月—2021年6月随机遴选该院急诊确诊的糖尿病酮症酸中毒患者62例展开对照研究,遵照随机数表法将所选患者划分为对照组和观察组。两组患者分别于治疗期间应用常规急救护理及综合急救护理,对比评估两组血糖水平、并发症发生概率、生活质量、护理满意度。结果干预前,对照组、观察组血糖水平对比,差异无统计学意义(P>0.05);干预后,观察组空腹血糖、餐后2 h血糖及糖化血红蛋白水平低于对照组,差异有统计学意义(P<0.05);参照对照组的25.81%,观察组并发症发生率3.23%较低,差异有统计学意义(P<0.05);相较于对照组,观察组各项生活质量、护理满意度均较高,差异有统计学意义(P<0.05)。结论急诊糖尿病酮症酸中毒患者接受综合急救护理对于降低并发症发生率具有积极意义,有利于加速患者康复进程,可以有效控制患者血糖水平。

福州市某猪场不同日龄猪伪狂犬病血清学检测与评价

[目的]调查福州市某规模化猪场经常出现伪狂犬病例的原因。[方法]采集血样133份,其中公猪8份、经产母猪25份、后备母猪20份、10~30日龄仔猪20份、40~60日龄小猪20份、80~100日龄肉猪20份、120日龄以上肉猪20份,进行伪狂犬病gB和gE抗体检测。[结果]7组猪伪狂犬病gB抗体阳性率分别为100%、100%、95%、100%、70%、90%和40%;伪狂犬病gE抗体阳性率分别为37.5%、56%、25%、45%、30%、5%和15%。[评价]该猪场种猪伪狂犬病疫苗免疫抗体正常,免疫程序合理;但肉猪的伪狂犬病疫苗免疫抗体不均匀,免疫程序不合理;同时,该猪场存在较严重的伪狂犬病野毒感染,需通过调整伪狂犬病疫苗免疫程序以及淘汰gE抗体阳性种猪来净化猪场的伪狂犬病。

福建省猪病例中土源性人畜共患寄生虫感染情况调查

为了解目前福建省猪场中土源性人畜共患寄生虫感染情况,对300头来自福建省各地的病死猪进行似蚓蛔虫、十二指肠钩口线虫、结肠小袋纤毛虫、隐孢子虫以及溶组织内阿米巴虫等寄生虫进行检查。结果显示:300头病死猪中检出92头有结肠小袋纤毛虫感染,检出率为30.7%;1头有隐孢子虫感染,检出率为0.3%;未检出似蚓蛔虫、十二指肠钩口线虫和溶组织内阿米巴虫。在生产实践中,要加强饲养管理和猪粪便的无害化处理措施,以便能更好地做好猪场土源性人畜共患寄生虫病的防控工作。

湿性老年性黄斑变性的抗血管内皮生长因子治疗探讨

目的探讨湿性老年性黄斑变性的抗血管内皮生长因子(VEGF)治疗效果。方法选取福建医科大学附属协和医院2019年1月至2020年12月收治的80例湿性老年性黄斑变性患者,根据随机数字表法分为两组。对照组40例采用雷珠单抗治疗,观察组40例采用康柏西普治疗,均治疗3个月。对比患者VEGF、C反应蛋白(CRP)、早期糖尿病视网膜病变治疗研究(ETDRS)视力表视力、眼压(IOP)、黄斑区厚度。结果治疗前两组VEGF、CRP水平比较,差异无统计学意义(P>0.05);治疗后两组患者CRP、VEGF水平均低于治疗前,观察组低于对照组,差异有统计学意义(P<0.05);治疗前两组IOP、ETDRS视力比较,差异无统计学意义(P>0.05);治疗后观察组患者ETDRS高于对照组、IOP低于对照组,差异有统计学意义(P<0.05);治疗前两组患者黄斑区厚度比较,差异无统计学意义(P>0.05);两组治疗后黄斑区厚度低于治疗前,且观察组患者黄斑区厚度低于对照组,差异有统计学意义(P<0.05)。结论康柏西普治疗湿性老年性黄斑变性可有效改善患者VEGF、CRP水平、黄斑区厚度,提高视力,值得临床推广。