There are various clinical treatments for traumatic brain injury,including surgery,drug therapy,and rehabilitation therapy;howeve r,the therapeutic effects are limited.Scaffolds combined with exosomes represent a prom...There are various clinical treatments for traumatic brain injury,including surgery,drug therapy,and rehabilitation therapy;howeve r,the therapeutic effects are limited.Scaffolds combined with exosomes represent a promising but challenging method for improving the repair of traumatic brain injury.In this study,we determined the ability of a novel 3D-printed collagen/chitosan scaffold loaded with exosomes derived from neural stem cells pretreated with insulin-like growth factor-1(3D-CC-INEXOS) to improve traumatic brain injury repair and functional recove ry after traumatic brain injury in rats.Composite scaffolds comprising collagen,chitosan,and exosomes derived from neural stem cells pretreated with insulin-like growth fa ctor-1(INEXOS) continuously released exosomes for 2weeks.Transplantation of 3D-CC-INExos scaffolds significantly improved motor and cognitive functions in a rat traumatic brain injury model,as assessed by the Morris water maze test and modified neurological seve rity scores.In addition,immunofluorescence staining and transmission electron microscopy showed that3D-CC-INExos implantation significantly improved the recove ry of damaged nerve tissue in the injured area.In conclusion,this study suggests that transplanted3D-CC-INExos scaffolds might provide a potential strategy for the treatment of traumatic brain injury and lay a solid foundation for clinical translation.展开更多
The development of the 6-layered cerebral neocortex is one of the most important events during nervous system development,and disturbances could result in various malformations,causing clinically intractable diseases,...The development of the 6-layered cerebral neocortex is one of the most important events during nervous system development,and disturbances could result in various malformations,causing clinically intractable diseases,such as epilepsy and cerebral palsy.Pre-plate splitting is the first developmental step of the cortical plate formation.Without correct pre-plate splitting,normal cerebral cortex structures are disturbed.The Reelin-Dab1 molecular pathway plays a critical role during cerebral cortex development,and deficiencies in this pathway result in failed pre-plate splitting and an inverted cortical plate.This paper summarizes findings involving Reelin and pre-plate splitting and further explores the precise role of Reelin during pre-plate splitting.展开更多
We present validation between total ozone from satellite and ground-based observations of the Dobson and Brewer spectrometers and ozone radiosonde at Zhongshan and Syowa Antarctic research stations, for September 2004...We present validation between total ozone from satellite and ground-based observations of the Dobson and Brewer spectrometers and ozone radiosonde at Zhongshan and Syowa Antarctic research stations, for September 2004 to March 2009. Results show that mean bias error between Zhongshan (Syowa) and Ozone Monitor Instrument Total Ozone Mapping Spectrometer (OMI-TOMS) data are 0.06%±3.32% ( 0.44%±2.41%); between it and OMI Multi Axis Differential Optical Absorption Spectroscopy (OMI-DOAS) data, the error is 0.34%±4.99% ( 0.22%±4.85%). Mean absolute bias error values of OMI-TOMS data are less than those of OMI-DOAS. This means that total ozone of OMI-TOMS is closer to ground-based observation than that of OMI-DOAS. Comparison between direct observational total ozone of ground-based and integrated ozone from the ozone profile measured by ozone radiosonde shows that ozone amount calculated with the Solar Backscatter Ultraviolet (SBUV) method above balloon burst height is similar to corresponding Microwave Limb Sounder (MLS) data. Therefore, MLS data can be substituted with SBUV data to estimate ozone amount above that level. Mean bias error of the MLS ozone column is 2% compared with the ozonesonde column, with standard deviation within 9.5%. Comparison of different layers from ozone profiler and MLS data indicates that at the 215 hPa layer, the MLS ozone value is high, with relative deviation more than 20%. At the 100 hPa and 68 hPa layers, the MLS ozone value is also high. This deviation is mainly in spring, during Antarctic ozone hole appearance. In this period, at the height of severe ozone loss, relative deviation of MLS ozone values is especially large.展开更多
Objective:Hepatocellular carcinoma(HCC)has a high rate of postoperative recurrence and lacks an effective treatment to prevent recurrence.This study aims to investigate the efficacy and safety of anlotinib in postoper...Objective:Hepatocellular carcinoma(HCC)has a high rate of postoperative recurrence and lacks an effective treatment to prevent recurrence.This study aims to investigate the efficacy and safety of anlotinib in postoperative adjuvant therapy for HCC patients with high-risk recurrence factors.Methods:For this multicenter,retrospective study,we recruited 63 HCC patients who received either anlotinib(n=27)or transcatheter arterial chemoembolization(TACE)(n=36)from six research centers in China between March 2019 and October 2020.The primary endpoint was disease-free survival(DFS)and the secondary endpoints were overall survival(OS)and safety.Results:In this study,the median follow-up time was 25.9 and 26.8 months in the anlotinib and TACE groups,respectively.There was no significant difference in the median DFS between the anlotinib[26.8 months,95%confidence interval(95%CI):6.8-NE]and TACE groups(20.6 months,95%CI:8.4-NE).The 12-month OS rates in the anlotinib and TACE groups were 96.3%and 97.2%,respectively.In the anlotinib group,19 of 27patients(70.4%)experienced treatment-emergent adverse events,with the most common events(≥10%)being hypertension(22.2%)and decreased platelet count(22.2%).Conclusions:The results indicate that anlotinib,as a new,orally administered tyrosine kinase inhibitor,has the same efficacy as TACE,and side effects can be well controlled.展开更多
Ovarian cancer(OC)poses a significant challenge in modern gynecologic oncology,both diagnostically and therapeutically.According to the American Cancer Society,an estimated 21,000 new cases of OC were reported in the ...Ovarian cancer(OC)poses a significant challenge in modern gynecologic oncology,both diagnostically and therapeutically.According to the American Cancer Society,an estimated 21,000 new cases of OC were reported in the United States alone in 20211.The most prevalent subtype of OC87,highgrade serous(HGS),is characterized by heightened genomic instability and defects in DNA damage response(DDR)pathways,which contribute to disease development and progression2.Notably,approximately 50%of HGS ovarian cancer(HGSOC)patients exhibit homologous recombination repair(HRR)defects(HRDs)3.展开更多
基金supported by the National Major Scientific and Technological Special Project for Significant New Drugs Development,No.2019ZX09301-147 (to LXZ)。
文摘There are various clinical treatments for traumatic brain injury,including surgery,drug therapy,and rehabilitation therapy;howeve r,the therapeutic effects are limited.Scaffolds combined with exosomes represent a promising but challenging method for improving the repair of traumatic brain injury.In this study,we determined the ability of a novel 3D-printed collagen/chitosan scaffold loaded with exosomes derived from neural stem cells pretreated with insulin-like growth factor-1(3D-CC-INEXOS) to improve traumatic brain injury repair and functional recove ry after traumatic brain injury in rats.Composite scaffolds comprising collagen,chitosan,and exosomes derived from neural stem cells pretreated with insulin-like growth fa ctor-1(INEXOS) continuously released exosomes for 2weeks.Transplantation of 3D-CC-INExos scaffolds significantly improved motor and cognitive functions in a rat traumatic brain injury model,as assessed by the Morris water maze test and modified neurological seve rity scores.In addition,immunofluorescence staining and transmission electron microscopy showed that3D-CC-INExos implantation significantly improved the recove ry of damaged nerve tissue in the injured area.In conclusion,this study suggests that transplanted3D-CC-INExos scaffolds might provide a potential strategy for the treatment of traumatic brain injury and lay a solid foundation for clinical translation.
基金the Project of Abroad Researcher Foundation of Heilongjiang Province,No.LC07C17
文摘The development of the 6-layered cerebral neocortex is one of the most important events during nervous system development,and disturbances could result in various malformations,causing clinically intractable diseases,such as epilepsy and cerebral palsy.Pre-plate splitting is the first developmental step of the cortical plate formation.Without correct pre-plate splitting,normal cerebral cortex structures are disturbed.The Reelin-Dab1 molecular pathway plays a critical role during cerebral cortex development,and deficiencies in this pathway result in failed pre-plate splitting and an inverted cortical plate.This paper summarizes findings involving Reelin and pre-plate splitting and further explores the precise role of Reelin during pre-plate splitting.
基金supported by the Chinese Polar Environment Comprehensive Investigation and Assessment Programs (Grant no.JDZX20110019)
文摘We present validation between total ozone from satellite and ground-based observations of the Dobson and Brewer spectrometers and ozone radiosonde at Zhongshan and Syowa Antarctic research stations, for September 2004 to March 2009. Results show that mean bias error between Zhongshan (Syowa) and Ozone Monitor Instrument Total Ozone Mapping Spectrometer (OMI-TOMS) data are 0.06%±3.32% ( 0.44%±2.41%); between it and OMI Multi Axis Differential Optical Absorption Spectroscopy (OMI-DOAS) data, the error is 0.34%±4.99% ( 0.22%±4.85%). Mean absolute bias error values of OMI-TOMS data are less than those of OMI-DOAS. This means that total ozone of OMI-TOMS is closer to ground-based observation than that of OMI-DOAS. Comparison between direct observational total ozone of ground-based and integrated ozone from the ozone profile measured by ozone radiosonde shows that ozone amount calculated with the Solar Backscatter Ultraviolet (SBUV) method above balloon burst height is similar to corresponding Microwave Limb Sounder (MLS) data. Therefore, MLS data can be substituted with SBUV data to estimate ozone amount above that level. Mean bias error of the MLS ozone column is 2% compared with the ozonesonde column, with standard deviation within 9.5%. Comparison of different layers from ozone profiler and MLS data indicates that at the 215 hPa layer, the MLS ozone value is high, with relative deviation more than 20%. At the 100 hPa and 68 hPa layers, the MLS ozone value is also high. This deviation is mainly in spring, during Antarctic ozone hole appearance. In this period, at the height of severe ozone loss, relative deviation of MLS ozone values is especially large.
基金supported by Key Program,National Natural Science Foundation of China(No.81930016)Natural Science Foundation of Zhejiang Province(No.LY22H160046)Key Research&Development Plan of Zhejiang Province(No.2019C03050)。
文摘Objective:Hepatocellular carcinoma(HCC)has a high rate of postoperative recurrence and lacks an effective treatment to prevent recurrence.This study aims to investigate the efficacy and safety of anlotinib in postoperative adjuvant therapy for HCC patients with high-risk recurrence factors.Methods:For this multicenter,retrospective study,we recruited 63 HCC patients who received either anlotinib(n=27)or transcatheter arterial chemoembolization(TACE)(n=36)from six research centers in China between March 2019 and October 2020.The primary endpoint was disease-free survival(DFS)and the secondary endpoints were overall survival(OS)and safety.Results:In this study,the median follow-up time was 25.9 and 26.8 months in the anlotinib and TACE groups,respectively.There was no significant difference in the median DFS between the anlotinib[26.8 months,95%confidence interval(95%CI):6.8-NE]and TACE groups(20.6 months,95%CI:8.4-NE).The 12-month OS rates in the anlotinib and TACE groups were 96.3%and 97.2%,respectively.In the anlotinib group,19 of 27patients(70.4%)experienced treatment-emergent adverse events,with the most common events(≥10%)being hypertension(22.2%)and decreased platelet count(22.2%).Conclusions:The results indicate that anlotinib,as a new,orally administered tyrosine kinase inhibitor,has the same efficacy as TACE,and side effects can be well controlled.
基金funded by the National Natural Science Foundation of China(Grant No.82073129)the Ningxia Key Research and Development Program(Grant No.2023BEG02037)+3 种基金the Chongqing Science and Technology Bureau(Grant Nos.CSTB2023NSCQ-MSX1030,cstc-2021jxjl130032,and cstc2022jxjl20039)the Chongqing Health Commission(Grant Nos.2023ZDXM029 and 2023MSXM043)the Project for Enhancing Scientific Research Capabilities of Chongqing University Cancer Hospital(Grant No.2023nlts009)the Beijing Kangmeng Charity Foundation(Grant No.BJHA-CRP-089)。
文摘Ovarian cancer(OC)poses a significant challenge in modern gynecologic oncology,both diagnostically and therapeutically.According to the American Cancer Society,an estimated 21,000 new cases of OC were reported in the United States alone in 20211.The most prevalent subtype of OC87,highgrade serous(HGS),is characterized by heightened genomic instability and defects in DNA damage response(DDR)pathways,which contribute to disease development and progression2.Notably,approximately 50%of HGS ovarian cancer(HGSOC)patients exhibit homologous recombination repair(HRR)defects(HRDs)3.
