Expression of ST13 in colorectal cancer and adjacent normal tissues

AIM: To investigate the in situ expression of suppressionof tumorigenecity 13 (ST13) mRNA in both colorectal cancer and adjacent normal tissues.METHODS: Colorectal cancer cell lines SW1116, SW620and CoLo205 were enrolled to confirm the feasibility of the in situ hybridization procedure. Seven colorectal cancer and adjacent normal tissues were included for RNA-RNA in situ hybridization.RESULTS: The expression of ST13 in the seven normal colon tissues was positive and the positive signals appeared in mucosal cells. Only three of the seven colorectal cancer tissues had positive hybridization signals that appeared in adenocarcinoma cells.CONCLUSION: The expression of ST13 decreases in colorectal cancer tissue compared with that in adjacent normal tissue. ST13 is mostly expressed in colorectal epithelia and adenocarcinoma cells.

Amino acid uptake in arterio-venous serum of normal and cancerous colon tissues

AIM:To investigate the difference of amino acid uptake between normal and cancerous colon tissues.METHODS:Sixteen patients with colon cancer were enrlled in our study.Blood samples were taken during operations,serum amion acid concentrations of blood from cancerous or normal colon were analyzed.Amino acid uptake rate was calculated by the A-V difference and evaluated statistically.RESULTS:Except for methionine,the uptake rate of amino acids in cancer was higher than that in normal colon (25.01% vs -2.29%,p<0.01).The amino acid uoptake rate did not correlate to the size of tumor mass (p>0.05).There was no staistical significance in the amino acid uptake rate according to the Dukes stage,though it was higher in patients with Dukes stage C or D than that with Dukes stage B(P>0.05).CONCLUSION:Abnormal synthetic metabolism of colon cancer may contribute to its higher amino caid uptake rate than that of normal colon.