超声联合血清标志物诊断慢性心力衰竭的价值

目的探讨心力衰竭超声指数联合血清半乳糖凝集素-3(Gal-3)、亲环素A 在慢性心力衰竭(CHF)诊断中的的价值。方法选取2015年1月—2018 年1月唐山市工人医院收治的312 例CHF 患者作为观察组。选取同期于该院体检的60例健康人群作为对照组。根据左室射血分数(LVEF)分为收缩性组和舒张性组。根据纽约心脏病学会心功能分级(NHYA)将患者分为NHYA Ⅰ、Ⅱ、Ⅲ及Ⅳ级组。检测不同组患者心力衰竭超声指数、血清Gal-3、亲环素A 水平,分析其与心功能的相关性。结果对照组和NYHA Ⅰ、Ⅱ、Ⅲ及Ⅳ级组患者的血清Gal-3、亲环素A、心力衰竭超声指数及LVEF 比较,差异有统计学意义(P <0.05),分级越高,Gal-3、亲环素A 和心力衰竭超声指数越高。收缩性心力衰竭患者血清Gal-3、亲环素A 和心力衰竭超声指数高于舒张性心力衰竭患者(P <0.05);血清Gal-3、亲环素A、心力衰竭超声指数与LVEF 呈负相关(r=-0.635、-0.622 和-0.628,均P <0.05),与心功能NHYA 分级呈正相关(r=0.745、0.834 和0.867,均P <0.05)。结论心力衰竭超声指数联合血清Gal-3、亲环素A 检测可进一步提高慢性心力衰竭的临床诊断水平,可作为评价患者心功能的重要指标.

Episodic crustal growth in the Tanzania Craton:evidence from Nd isotope compositions

The analysis of available Nd isotope data from the Tanzania Craton places important constraints on the crest-mantle separation ages,and events marking juvenile crustal addition and crustal recycling.Nd model ages date the oldest crust extraction to 3.16 Ga in the Tanzania Craton,although a rock record of such antiquity is yet to be found there.The most significant period of juvenile crustal addition as well as crustal recycling is 2.7-2.6 Ga.The Nd isotopes of marie samples show that chemical heterogeneity existed in the mantle beneath the Tanzania Craton,with some samples originating from significantly depleted mantle, and most samples originating from the mixture of primitive mantle and depleted mantle.The Nd isotope section reveals significant differences in Nd isotopes between the north eraton and central craton;compared to the north craton,the central craton yields a Nd model age that is approximately 100 Ma older, and its εNd(t)values are more negative,indicating that the two parts of the craton have different mantle source regions.Different types of granitoids are distributed in the Tanzania Craton,such as high-K and low-Al granite,ealc-alkaline granite,peraluminous granite and transitional types of tonalite-trondhjemite- granodiorites (TTGs).Most of the granitoids formed later than the marie rocks in syn-collision and postcollision events.

Short-lived AIM2 Inflammasome Activation Relates to Chronic MCMV Infection in BALB/c Mice

Absent in melanoma 2(AIM2)inflammasome is a crucial link bridging the innate host defense and the subsequent adaptive immunity when activated by exogenous double stranded DNA(dsDNA).Through establishing models of disseminated murine cytomegalovirus(MCMV)infection in BALB/c and C57BL/6 mice,we evaluated dynamic expression of AIM2 inflammasome components and its relationship with pathological damage and viral replication,trying tofigure out whether AIM2 inflammasome is related to the chronic mechanism of MCMV.BALB/c and C57BL/6 mice were sacrificed on day 0,1,3,7,14 and 28 post infection.Expression levels of AIM2,pro-caspase-1,caspase-1 p20,pro-IL1β and mature IL1β in primary peritoneal macrophages(PMs)and spleens were detected by Western blotting.Contents of IL18 in the serum were detected by ELISA.Pathological examinations of livers were performed,and mRNA levels of MCMV glycoprotein B(gB)in salivary glands also assessed.Results showed that expression levels of AIM2 in PMs and spleens of C57BL/6 mice increased on day 3,even continued to day 28;caspase-1 p20 and mature IL1β increased on day 7,14 and 28;the persistently high expression of IL1β in the serum started on day 1,showing a double peak curve.As for BALB/c mice,expression of AIM2 in PMs increased on day 1 and day 7,while contents of AIM2 in spleens increased on day 1 and day 3;caspase-1 p20 and mature ILip merely increased 7 days fter infection.Thereafter,expression levels of AIM2,caspase-1 p20,mature IL1β and IL18 were limited;the duration of AIM2 inflammasome activation in BALB/c mice was much shorter than that in C57BL/6 mice.The severer pathological damage and more viral replications in BALB/c mice further proved the deficient antiviral immunity to MCMV.In conclusion,the activation of AIM2 inflammasome in BALB/c mice was short-lived,which is quite possibly related to the chronicity of MCMV infection.

Maternal Murine Cytomegalovirus Infection during Pregnancy Up-regulates the Gene Expression of Toll-like Receptor 2 and 4 in Placenta

Increasing evidence has revealed that maternal cytomegalovirus （CMV） infection may be associated with neurodevelopmental disorders in offspring. Potential relevance between the placental inflammation and CMV-related autism has been reported by clinical observation. Meanwhile, abnormal expression of Toll-like receptor 2 （TLR2） and TLR4 in placenta of patients with chorioamnionitis was observed in multiple studies. IL-6 and IL- 10 are two important maternal inflammatory mediators involved in neurodevelopmental disorders. To investigate whether murine CMV （MCMV） infection causes alterations in placental IL-6/10 and TLR2/4 levels, we analyzed the dynamic changes in gene expression of TLR2/4 and IL-6/10 in placentas following acute MCMV infection. Mouse model of acute MCMV infection during pregnancy was created, and pre-pregnant MCMV infected, lipopolysaccharide （LPS）-treated and uninfected mice were used as controls. At E13.5, E 14.5 and E 18.5, placentas and fetal brains were harvested and mRNA expression levels of placental TLR2/4 and IL-6/10 were analyzed. The results showed that after acute MCMV infection, the expression levels of placental TLR2/4 and IL-6 were elevated at E13.5, accompanied by obvious placental inflammation and reduction of placenta and fetal brain weights. However, LPS 50 ktg/kg could decrease the IL-6 expression at E13.5 and E14.5. This suggests that acute MCMV infection during pregnancy could up-regulate the gene expression of TLR2/4 in placental trophoblasts and activate them to produce more pro- inflammatory cytokine IL-6. High dose of LPS stimulation （50 gg/kg） during pregnancy can lead to down-regulation of IL-6 levels in the late stage. Imbalance of IL-6 expression in placenta might be associated with the neurodevelopmental disorders in progeny.

前哨淋巴结阳性早期乳腺癌患者腋窝非前哨淋巴结转移的危险因素分析

目的通过分析前哨淋巴结(SLN)活检阳性的早期乳腺癌患者的临床资料与病理特征,探讨其腋窝非SLN转移的危险因素。方法选取2017年7月—2019年7月于锦州医科大学附属第一医院及鞍山市中心医院就诊的SLN活检阳性并行腋窝淋巴结清扫术的早期乳腺癌患者116例,单因素分析其临床和病理资料,多因素Logistic回归分析SLN阳性的早期乳腺癌患者腋窝非SLN转移的危险因素。结果单因素分析显示,两组患者肿瘤直径、组织学分级、脉管内癌栓、分子分型及SLN阳性数目比较,差异有统计学意义(P<0.05)。多因素Logistic回归分析显示,肿瘤直径[O^R=1.784(95%CI:1.091,2.916),P<0.05]、有脉管癌栓[O^R=0.301(95%CI:0.113,0.804),P<0.05]及SLN阳性数≥3枚[O^R=4.977(95%CI:1.114,22.239),P<0.05]是影响腋窝非SLN转移的危险因素。SLN阳性数目≥3枚患者腋窝非SLN转移风险是SLN阳性数目1枚患者的4.977倍。结论对于SLN活检阳性的早期乳腺癌患者,肿瘤直径越小、无脉管内癌栓及SLN阳性数目越少,腋窝非SLN转移可能性越低,对免行腋窝淋巴结清扫有指示意义。

急性进展性后部型早产儿视网膜病变的治疗研究进展

急性进展性后部型早产儿视网膜病变(aggressive posterior retinopathy of prematurity,AP-ROP)是一种少见且进展很快的严重性早产儿视网膜病变,可不遵循1~3期的经典病程,而迅速进展为5期病变,最终导致不可逆的视力丧失。目前常用的治疗方式有激光光凝、玻璃体腔内注射抗血管内皮生长因子(VEGF)、玻璃体切除术。本文将从以上三个方面入手,对AP-ROP的治疗方式进行综述。

Value of serum hk10 and DJ-1 levels in patients with ovarian cancer for evaluating the malignant biology of tumor

Objective:To discuss the value of serum hk10 and DJ-1 levels in patients with ovarian cancer for evaluating the malignant biology of tumor.Methods:A total of80 patients with ovarian cancer who underwent surgical treatment in our hospital between August 2015 and February 2018 were selected as ovarian cancer group, and 67 patients with ovarian cyst were selected as ovarian cyst group. The differences in the levels of hk10 and DJ-1 in preoperative serum as well as the expression of ovarian cancer-related proliferation genes and invasion genes in intraoperative lesion tissue were compared between the two groups. Correlation analysis was used to assess the inner link of serum hk10 and DJ-1 levels with tumor malignancy in patients with ovarian cancer.Results: Serum hk10 and DJ-1 levels of ovarian cancer group were higher than those of ovarian cyst group;proliferation genes Clusterin, FUNDC1, AKT, TNFAIP8 and HBXIP mRNA expression in lesion tissues were higher than those of ovarian cyst group;invasion genes Beclin1, BRMS1 and SIRT2 mRNA expression in lesion tissues were lower than those of ovarian cyst group whereas EFEMP1 mRNA expression was higher than that of ovarian cyst group. The Pearson test showed that serum hk10 and DJ-1 levels in ovarian cancer were directly correlated with the expression of proliferation genes and invasion genes. Conclusion: hk10 and DJ-1 are highly expressed in serum of patients with ovarian cancer, and their levels are directly correlated with the proliferation and invasion activity of cancer cells.

Evidence Based Medicine in Psychosomatics:Prospects and Challenges

Evidence-based Medicine(EBM)means"Medicine based on scientific Evidence"[1].Its core idea is that medical staff should use the latest and best scientific Evidence obtained in clinical research prudently,clearly and wisely.It also combine the professional skills and clinical experience of doctors,take into account individual values and expectations of patients and combine the three perfectly to formulate the best treatment measures for each patient[2].

Recent development of three-dimension printed graphene oxide and MXene-based energy storage devices

The research for three-dimension(3D)printing carbon and carbide energy storage devices has attracted widespread exploration interests.Being designable in structure and materials,graphene oxide(GO)and MXene accompanied with a direct ink writing exhibit a promising prospect for constructing high areal and volume energy density devices.This review not only summarizes the recent advances in 3D printing energy storage devices including printing methods,ink rheological properties,and different energy storage systems,but also discusses the printing methods related to energy storage.In addition,the binder or additive free of two-dimensional carbide materials is quite important for the present electrochemical energy storage devices,which also are presented.

植物响应逆境胁迫过程中的染色质3D结构重塑

Being sessile,plants evolve different acclimation strategies at both the transcriptional and post-translational levels to cope with adverse environmental conditions[1].Several recent studies[2–9]have shown that gene expression at transcriptional level under abiotic stress conditions is associated with highly dynamic threedimensional(3D)chromatin reorganization.

Active constituents and mechanisms of Respiratory Detox Shot, a traditional Chinese medicine prescription, for COVID-19 control and prevention: Network-molecular docking-LC–MS^E analysis

Objective: Lung-toxin Dispelling Formula No. 1, referred to as Respiratory Detox Shot(RDS), was developed based on a classical prescription of traditional Chinese medicine(TCM) and the theoretical understanding of herbal properties within TCM. Therapeutic benefits of using RDS for both disease control and prevention, in the effort to contain the coronavirus disease 2019(COVID-19), have been shown. However,the biochemically active constituents of RDS and their mechanisms of action are still unclear. The goal of the present study is to clarify the material foundation and action mechanism of RDS.Methods: To conduct an analysis of RDS, an integrative analytical platform was constructed, including target prediction, protein–protein interaction(PPI) network, and cluster analysis;further, the hub genes involved in the disease-related pathways were identified, and the their corresponding compounds were used for in vitro validation of molecular docking predictions. The presence of these validated compounds was also measured in samples of the RDS formula to quantify the abundance of the biochemically active constituents. In our network pharmacological study, a total of 26 bioinformatic programs and databases were used, and six networks, covering the entire Zang-fu viscera, were constructed to comprehensively analyze the intricate connections among the compounds-targets-disease pathways-meridians of RDS.Results: For all 1071 known chemical constituents of the nine ingredients in RDS, identified from established TCM databases, 157 passed drug-likeness screening and led to 339 predicted targets in the constituent–target network. Forty-two hub genes with core regulatory effects were extracted from the PPI network, and 134 compounds and 29 crucial disease pathways were implicated in the target–constitu ent–disease network. Twelve disease pathways attributed to the Lung–Large Intestine meridians, with six and five attributed to the Kidney–Urinary Bladder and Stomach–Spleen meridians, respectively. Onehundred and eighteen candidate constituents showed a high binding affinity with SARS-coronavirus-23-chymotrypsin-like protease(3 CLpro), as indicated by molecular docking using computational pattern recognition. The in vitro activity of 22 chemical constituents of RDS was validated using the 3 CLproinhibition assay. Finally, using liquid chromatography mass spectrometry in data-independent analysis mode,the presence of seven out of these 22 constituents was confirmed and validated in an aqueous decoction of RDS, using reference standards in both non-targeted and targeted approaches.Conclusion: RDS acts primarily in the Lung–Large Intestine, Kidney–Urinary Bladder and Stomach–Spleen meridians, with other Zang-fu viscera strategically covered by all nine ingredients. In the context of TCM meridian theory, the multiple components and targets of RDS contribute to RDS’s dual effects of healthstrengthening and pathogen-eliminating. This results in general therapeutic effects for early COVID-19 control and prevention.

The E3 ligase XBAT35 mediates thermoresponsive hypocotyl growth by targeting ELF3 for degradation in Arabidopsis

Plants are capable of coordination of their growth and development with ambient temperatures.EARLY FLOWERING3(ELF3), an essential component of the plant circadian clock, is also involved in ambient temperature sensing, as well as in inhibiting the expression and protein activity of the thermoresponsive regulator phytochrome interacting factor4(PIF4). The ELF3 activity is subjected to attenuation in response to warm temperature;however,how the protein level of ELF3 is regulated at warm temperature remains less understood. Here, we report that the E3 ligase XB3 ORTHOLOG 5 IN ARABIDOPSIS THALIANA, XBAT35, mediates ELF3 degradation. XBAT35 interacts with ELF3 and ubiquitinates ELF3. Loss-of-function mutation of XBAT35 increases the protein level of ELF3 and confers a short-hypocotyl phenotype under warm temperature conditions. Thus, our findings establish that XBAT35 mediates ELF3 degradation to lift the inhibition of ELF3 on PIF4 for promoting thermoresponsive hypocotyl growth in plants.

Weighted Co-Training for Cross-Domain Image SentimentClassification

Image sentiment classification, which aims to predict the polarities of sentiments conveyed by the images, has gained a lot of attention. Most existing methods address this problem by training a general classifier with certain visual features, ignoring the discrepancies across domains. In this paper, we propose a novel weighted co-training method for cross-domain image sentiment classification, which iteratively enlarges the labeled set by introducing new high-confidence classified samples to reduce the gap between the two domains. We train two sentiment classifiers with both the images and the corresponding textual comments separately, and set the similarity between the source domain and the target domain as the weight of a classifier. We perform extensive experiments on a real Flickr dataset to evaluate the proposed method, and the empirical study reveals that the weighted co-training method significantly outperforms some baseline solutions.

Automatic tracking of multi-fruit targets with oscillation under natural growth conditions

It is important for intelligent orchards to be able to achieve automatic monitoring of fruit growth information within a natural growing environment.The issue of how to track green and oscillating fruits under the influence of wind and farming operations is a key aspect of monitoring of the growth state of the fruit.In order to realize the accurate tracking of green fruit targets,a new method based on target tracking is proposed.First,an optical flow method is applied to realize the automatic detection of green fruit targets,and this lays the foundation for the accurate and automatic tracking of these targets.Then,Kalman and kernelized correlation filter(KCF)algorithms are applied to achieve multi-target tracking and prediction.In order to verify the performance of these different algorithms on various types of green fruit targets,experiments were carried out based on nine video sequences.The experimental results for the tracking of single,double and triple green fruit targets show that the average tracking success rates of the Kalman algorithm are 88.15%,82.30%and 53.10%,respectively,and those of the KCF algorithm are 94.07%,87.35%and 61.46%,respectively,meaning that the average tracking results from KCF are 5.92%,5.05%and 8.36%higher than those from the Kalman algorithm.The time consumed is also reduced by 35.40%,36.27%and 40.86%,respectively.The results show that it is feasible to apply the KCF algorithm to the tracking of green fruit targets.

The calcium-sensing receptor participates in testicular damage in streptozotocin-induced diabetic rats

Male infertility caused by testicular damage is one of the complications of diabetes mellitus. The calcium-sensing receptor （CaSR） is expressed in testicular tissues and plays a pivotal role in calcium homeostasis by activating cellular signaling pathways, but its role in testicular damage induced by diabetes remains unclear. A diabetic model was established by a single intraperitoneal injection of streptozotocin （STZ, 40 mg kg-1） in Wistar rats. Animals then received GdCl3 （an agonist of CaSR, 8.67 mg kg-1）, NPS-2390 （an antagonist of CaSR, 0.20 g kg-~）, or a combination of both 2 months after STZ injection. Diabetic rats had significantly lower testes weights and serum levels of testosterone compared to healthy rats, indicating testicular damage and dysfunction in STZ-induced diabetic rats. Compared with healthy controls, the testicular tissues of diabetic rats overexpressed the CaSR protein and had higher levels of malondialdehyde （MDA）, lower superoxide dismutase （SOD） and glutathione peroxidase （GSH-Px） activity, and higher numbers of apoptotic germ cells. The testicular tissues from diabetic rats also expressed lower levels of Bcl-2 and higher levels of Bax and cleaved caspase-3 in addition to higher phosphorylation rates of c-Jun NH2-terminal protein kinase （JNK）, p38, and extracellular signaling-regulated kinase （ERK） 1/2. The above parameters could be further increased or aggravated by the administration of GdCI3, but could be attenuated by injection of NPS-2390. In conclusion, the present results indicate that CaSR activation participates in diabetes-induced testicular damage, implying CaSR may be a potential target for protective strategies against diabetes-induced testicular damage and could help to prevent infertility in diabetic men.

Estimation of Nuclear DNA Content in Tannin-rich Medicinal Plant Cornus officinalis by Flow Cytometry

Objective The amount of nuclear DNA（C-value）is a key biodiversity character that provides strong unifying elements in revealing the phylogenetic regularity and relationship between genome size and functional traits for plant resource.The estimation of C-values could primarily extend our knowledge on the genetic background and genome diversity for medicinal plants,and thereby the variation of pharmacological constituents and phylogenetic mechanism of medicinal plant taxa will be revealed.However,a large number of medicinal plants（e.g.Cornus officinalis）typically contain a series of secondary metabolites,especially tannic acid,which would significantly affect the estimation of DNA content by flow cytometry（FCM）.Methodological discussions and improvement need to be made to solve this problem.Methods Two isolation buffers LB01 and Otto 1 were selected to prepare nuclear suspension with additional treatments of pre-soaking and centrifugation combination of gradient centrifugal force and duration.The best isolation and estimation methods were determined by FCM measurement in C.officinalis.Results The dry leaves were pre-soaked in Otto I buffer for 15 min and the Otto I nuclear suspension was centrifugated at 1.0×103 g for 2 min.The results showed that debris and nuclei were better separated and the scatterplots of good quality were obtained with low coefficient of variation（CV）.Contrarily,the nuclear DNA content of C.officinalis could not be accurately estimated for nuclei extracted by LB01 buffer.Finally,2C-value and genome size of C.officinalis were first estimated as 5.92 pg and 2893 Mbp,respectively.Conclusion The new methods proposed here are able to accurately estimate DNA content of C.officinalis,which provides valuable references for the estimation of genome size in other tannin-rich medicinal plants.